Restriction map for pF1KB6905
##################################
# NO CUT ENZYMES (  171 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AflIII      AgeI        AhdI        Alw44I      
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BglI        BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiHKAI     BsiWI       BsmAI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrGI       BssHII      Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstXI       BstZ17I     
Bsu36I      CelII       Cfr13I      ClaI        CpoI        
DraI        DraII       DraIII      DrdI        Eam1105I    
Eco47III    Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FbaI        
FseI        FspI        HgaI        Hin1I       HincII      
HindII      HpaI        KasI        KpnI        MaeII       
MfeI        MluI        MluNI       MroI        MscI        
MslI        MunI        NarI        NcoI        NdeI        
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SalI        SapI        Sau96I      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        Tth111I     TthHB8I     Van91I      
XbaI        XcmI        XhoI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AccII           6  CGCG                 CG^CG
AcsI          529  AAATTT               [GA]^AATT[CT]
AfaI           42  GTAC                 GT^AC
AflII         445  CTTAAG               C^TTAAG
ApoI          529  AAATTT               [GA]^AATT[CT]
BciVI         201  GGATAC               GTATCC(6/5)
BfaI          190  CTAG                 C^TAG
BfrI          445  CTTAAG               C^TTAAG
BglII         387  AGATCT               A^GATCT
BsaJI           5  CCGCGG               C^C[ACGT][ACGT]GG
BsiEI           8  CGGCCG               CG[GA][CT]^CG
BsmFI          20  GGGAC                GGGAC(10/14)
BssSI          49  CTCGTG               CACGAG(-5/-1)
BstNI          33  CCAGG                CC^[AT]GG
BstUI           6  CGCG                 CG^CG
BstYI         387  AGATCT               [GA]^GATC[CT]
CfoI           85  GCGC                 GCG^C
DdeI          334  CTCAG                C^T[ACGT]AG
DsaI            5  CCGCGG               C^C[GA][CT]GG
EagI            8  CGGCCG               C^GGCCG
EarI          217  GAAGAG               CTCTTC(1/4)
EclXI           8  CGGCCG               C^GGCCG
Eco52I          8  CGGCCG               C^GGCCG
EcoRII         33  CCAGG                ^CC[AT]GG
HaeII          84  GGCGCT               [GA]GCGC^[CT]
HhaI           85  GCGC                 GCG^C
HinP1I         85  GCGC                 G^CGC
HindIII       497  AAGCTT               A^AGCTT
Ksp632I       217  GAAGAG               CTCTTC(1/4)
KspI            5  CCGCGG               CCGC^GG
MaeI          190  CTAG                 C^TAG
MflI          387  AGATCT               [GA]^GATC[CT]
MspA1I          5  CCGCGG               C[AC]G^C[GT]G
MvaI           33  CCAGG                CC^[AT]GG
MvnI            6  CGCG                 CG^CG
NaeI           10  GCCGGC               GCC^GGC
NgoMIV         10  GCCGGC               G^CCGGC
RsaI           42  GTAC                 GT^AC
SacII           5  CCGCGG               CCGC^GG
SfaNI         466  GCATC                GCATC(5/9)
SmlI          445  CTTAAG               C^T[CT][GA]AG
Tsp45I        283  GTCAC                ^GT[GC]AC
TspRI         160  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         387  AGATCT               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AluI          443  AGCT                 AG^CT
              498  AGCT                
BcnI          185  CCCGG                CC^[GC]GG
              516  CCGGG               
BsaBI         124  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              388  GATCTGGATC          
BsiYI          11  CCGGCTGGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               45  CCGGCTCGTGG         
BslI           11  CCGGCTGGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               45  CCGGCTCGTGG         
BsrFI          10  GCCGGC               [GA]^CCGG[CT]
               44  ACCGGC              
Cac8I          10  GCCGGC               GC[ACGT]^[ACGT]GC
               14  GCTGGC              
Cfr10I         10  GCCGGC               [GA]^CCGG[CT]
               44  ACCGGC              
FauI           18  GCGGG                CCCGC(4/6)
               62  GCGGG               
HinfI         145  GATTC                G^A[ACGT]TC
              330  GATTC               
HphI           89  TCACC                GGTGA(8/7)
              556  TCACC               
MamI          124  GATTATGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
              388  GATCTGGATC          
MseI          370  TTAA                 T^TAA
              446  TTAA                
MwoI           62  GCGGGGGCGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               68  GCGGCGTGGGC         
NciI          185  CCCGG                CC^[GC]GG
              516  CCGGG               
NlaIV          25  GGCTCC               GG[ACGT]^[ACGT]CC
              226  GGAGCC              
TfiI          145  GATTC                G^A[AT]TC
              330  GATTC               
Tru9I         370  TTAA                 T^TAA
              446  TTAA                
Tsp509I       372  AATT                 ^AATT
              530  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (   24 enzymes) #
#########################################

AciI        AlwI        BbvI        BsrI        BssKI       
BstF5I      DpnII       EaeI        Fnu4HI      FokI        
HaeIII      HapII       HpaII       ItaI        MaeIII      
MboI        MboII       MnlI        MspI        NdeII       
NlaIII      Sau3AI      ScrFI       TseI        
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