Restriction map for pF1KB6896
 (Circular) (Six-base) MAPSORT of: pF1KB6896.seq  Check: 7942  from: 1  to: 3719



pF1KB6896  3719 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    148     148 
   Size:       3719
 
AccI GT'mk_AC

Cuts at:    757     757 
   Size:       3719
 
Acc65I G'GTAC_C

Cuts at:    735     735 
   Size:       3719
 
AclI AA'CG_TT

Cuts at:   3559    3559 
   Size:       3719
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    376     643    1544    1976    2723    2991     376 
   Size:        267     901     432     747     268    1104

  Fragments arranged by size: 

                1104     901     747     432     268     267
 
AflIII A'CryG_T

Cuts at:    169     583    1071    2175    3152     169 
   Size:        414     488    1104     977     736

  Fragments arranged by size: 

                1104     977     736     488     414
 
AgeI A'CCGG_T

Cuts at:   2107    2107 
   Size:       3719
 
AlwNI CAG_nnn'CTG

Cuts at:   1024    2591    1024 
   Size:       1567    2152
 
ApaLI G'TGCA_C

Cuts at:   2489    2489 
   Size:       3719
 
ApoI r'AATT_y

Cuts at:    723    3072    3328     723 
   Size:       2349     256    1114

  Fragments arranged by size: 

                2349    1114     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3719
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3719
 
AvaI C'yCGr_G

Cuts at:    739     739 
   Size:       3719
 
BamHI G'GATC_C

Cuts at:    744     744 
   Size:       3719
 
BanI G'GyrC_C

Cuts at:    735    1149    1397    1432     735 
   Size:        414     248      35    3022

  Fragments arranged by size: 

                3022     414     248      35
 
BanII G_rGCy'C

Cuts at:    704     733    1763     704 
   Size:         29    1030    2660

  Fragments arranged by size: 

                2660    1030      29
 
BbeI G_GCGC'C

Cuts at:   1153    1401    1153 
   Size:        248    3471
 
BbsI GAAGACnn'nnnn_

Cuts at:    364     586     364 
   Size:        222    3497
 
BbvCI CC'TCA_GC

Cuts at:    660     660 
   Size:       3719
 
BciVI GTATCCnnnnn_n'

Cuts at:   1617    2378    3719    1617 
   Size:        761    1341    1617

  Fragments arranged by size: 

                1617    1341     761
 
BclI T'GATC_A

Cuts at:   1239    1239 
   Size:       3719
 
BglI GCCn_nnn'nGGC

Cuts at:    445     445 
   Size:       3719
 
BglII A'GATC_T

Cuts at:    455    1234    3271     455 
   Size:        779    2037     903

  Fragments arranged by size: 

                2037     903     779
 
BlpI GC'TnA_GC

Cuts at:    316     831     316 
   Size:        515    3204
 
Bme1580I G_kGCm'C

Cuts at:    450    1344    1437    2493     450 
   Size:        894      93    1056    1676

  Fragments arranged by size: 

                1676    1056     894      93
 
BmgBI CAC'GTC

Cuts at:    172     172 
   Size:       3719
 
BmrI ACTGGGnnnn_n'

Cuts at:    224     931    1012    1347    3491     224 
   Size:        707      81     335    2144     452

  Fragments arranged by size: 

                2144     707     452     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    621    2086     621 
   Size:       1465    2254
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    166     403     426     166 
   Size:        237      23    3459

  Fragments arranged by size: 

                3459     237      23
 
Bpu10I CC'TnA_GC

Cuts at:    572     660     572 
   Size:         88    3631
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    895    1774    2266    2564     895 
   Size:        879     492     298    2050

  Fragments arranged by size: 

                2050     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    505    3423     505 
   Size:       2918     801
 
BsaAI yAC'GTr

Cuts at:    586    1072     586 
   Size:        486    3233
 
BsaBI GATnn'nnATC

Cuts at:      6    1233    3270       6 
   Size:       1227    2037     455

  Fragments arranged by size: 

                2037    1227     455
 
BsaHI Gr'CG_yC

Cuts at:   1150    1398    2100    3596    1150 
   Size:        248     702    1496    1273

  Fragments arranged by size: 

                1496    1273     702     248
 
BsaWI w'CCGG_w

Cuts at:    908    1429    2107    2381    2528    2939     908 
   Size:        521     678     274     147     411    1688

  Fragments arranged by size: 

                1688     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    388     418     478     388 
   Size:         30      60    3629

  Fragments arranged by size: 

                3629      60      30
 
BseYI C'CCAG_C

Cuts at:    201     285    1144    1902    2479    2975     201 
   Size:         84     859     758     577     496     945

  Fragments arranged by size: 

                 945     859     758     577     496      84
 
BsiEI CG_ry'CG

Cuts at:    104    1261    1307    2515     104 
   Size:       1157      46    1208    1308

  Fragments arranged by size: 

                1308    1208    1157      46
 
BsiHKAI G_wGCw'C

Cuts at:    733    1511    1701    2493     733 
   Size:        778     190     792    1959

  Fragments arranged by size: 

                1959     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    450     704     733    1344    1437    1511    1701    1763    2493 
   Size:        254      29     611      93      74     190      62     730

Cuts at:   2493     450 
   Size:       1676

  Fragments arranged by size: 

                1676     730     611     254     190      93      74      62
                  29
 
BspEI T'CCGG_A

Cuts at:   2939    2939 
   Size:       3719
 
BspHI T'CATG_A

Cuts at:   2895    2895 
   Size:       3719
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    148     317     545     769     983    1285    1666     148 
   Size:        169     228     224     214     302     381    2201

  Fragments arranged by size: 

                2201     381     302     228     224     214     169
 
BsrBI CCG'CTC

Cuts at:     50    2011    2065    3549    3714      50 
   Size:       1961      54    1484     165      55

  Fragments arranged by size: 

                1961    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1631    1631 
   Size:       3719
 
BsrFI r'CCGG_y

Cuts at:   1717    1898    2107    1717 
   Size:        181     209    3329

  Fragments arranged by size: 

                3329     209     181
 
BssSI C'ACGA_G

Cuts at:   1990    2348    1990 
   Size:        358    3361
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1096    1096 
   Size:       3719
 
BstBI TT'CG_AA

Cuts at:   2080    2080 
   Size:       3719
 
BstXI CCAn_nnnn'nTGG

Cuts at:    144     144 
   Size:       3719
 
BstYI r'GATC_y

Cuts at:      1     179     416     455     744    1211    1234    1569    1815 
   Size:        178     237      39     289     467      23     335     246

Cuts at:   1815    2827    2913    2925    3271       1 
   Size:       1012      86      12     346     449

  Fragments arranged by size: 

                1012     467     449     346     335     289     246     237
                 178      86      39      23      12
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1669    1850    2013    1669 
   Size:        181     163    3375

  Fragments arranged by size: 

                3375     181     163
 
BtsI GCAGTG_nn'

Cuts at:    483     962     483 
   Size:        479    3240
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    543     578     543 
   Size:         35    3684
 
DraI TTT'AAA

Cuts at:    718     718 
   Size:       3719
 
DrdI GACnn_nn'nnGTC

Cuts at:   1425    2283    1425 
   Size:        858    2861
 
EaeI y'GGCC_r

Cuts at:    122    1304    1478    1869    1896    3241     122 
   Size:       1182     174     391      27    1345     600

  Fragments arranged by size: 

                1345    1182     600     391     174      27
 
EagI C'GGCC_G

Cuts at:   1304    1304 
   Size:       3719
 
EarI CTCTTCn'nnn_

Cuts at:   1742    1952    1742 
   Size:        210    3509
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1927    2237    2383    3529    1927 
   Size:        310     146    1146    2117

  Fragments arranged by size: 

                2117    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    731     731 
   Size:       3719
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    166     376     403     426     643    1544    1976    2723    2991 
   Size:        210      27      23     217     901     432     747     268

Cuts at:   2991     166 
   Size:        894

  Fragments arranged by size: 

                 901     894     747     432     268     217     210      27
                  23
 
EcoO109I rG'GnC_Cy

Cuts at:    483     642     858     483 
   Size:        159     216    3344

  Fragments arranged by size: 

                3344     216     159
 
EcoRI G'AATT_C

Cuts at:    723     723 
   Size:       3719
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1738    1738 
   Size:       3719
 
FspI TGC'GCA

Cuts at:   1500    1500 
   Size:       3719
 
HaeII r_GCGC'y

Cuts at:   1153    1401    2423    3402    1153 
   Size:        248    1022     979    1470

  Fragments arranged by size: 

                1470    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    688     720    1561    1591    1593    1623     688 
   Size:         32     841      30       2      30    2784

  Fragments arranged by size: 

                2784     841      32      30      30       2
 
HincII GTy'rAC

Cuts at:    498     758     498 
   Size:        260    3459
 
KasI G'GCGC_C

Cuts at:   1149    1397    1149 
   Size:        248    3471
 
KpnI G_GTAC'C

Cuts at:    739     739 
   Size:       3719
 
MluI A'CGCG_T

Cuts at:   3152    3152 
   Size:       3719
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2390    2574    2390 
   Size:        184    3535
 
MscI TGG'CCA

Cuts at:   1480    1480 
   Size:       3719
 
MslI CAynn'nnrTG

Cuts at:   1708    1835    1708 
   Size:        127    3592
 
MspA1I CmG'CkG

Cuts at:    686     830    1144    1504    2517    2762     686 
   Size:        144     314     360    1013     245    1643

  Fragments arranged by size: 

                1643    1013     360     314     245     144
 
NaeI GCC'GGC

Cuts at:   1900    1900 
   Size:       3719
 
NarI GG'CG_CC

Cuts at:   1150    1398    1150 
   Size:        248    3471
 
NgoMIV G'CCGG_C

Cuts at:   1898    1898 
   Size:       3719
 
NspI r_CATG'y

Cuts at:    560     772    1272    2179     560 
   Size:        212     500     907    2100

  Fragments arranged by size: 

                2100     907     500     212
 
PciI A'CATG_T

Cuts at:   2175    2175 
   Size:       3719
 
PmeI GTTT'AAAC

Cuts at:    718     718 
   Size:       3719
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2858    2890    2858 
   Size:         32    3687
 
PpuMI rG'GwC_Cy

Cuts at:    642     642 
   Size:       3719
 
PstI C_TGCA'G

Cuts at:    542     691     766    1451     542 
   Size:        149      75     685    2810

  Fragments arranged by size: 

                2810     685     149      75
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3719
 
PvuII CAG'CTG

Cuts at:    686    1144    1504     686 
   Size:        458     360    2901

  Fragments arranged by size: 

                2901     458     360
 
RsrII CG'GwC_CG

Cuts at:   1914    1914 
   Size:       3719
 
SacI G_AGCT'C

Cuts at:    733     733 
   Size:       3719
 
SalI G'TCGA_C

Cuts at:    756     756 
   Size:       3719
 
SapI GCTCTTCn'nnn_

Cuts at:   1742    1952    1742 
   Size:        210    3509
 
SbfI CC_TGCA'GG

Cuts at:    766     766 
   Size:       3719
 
ScaI AGT'ACT

Cuts at:    222    3236     222 
   Size:       3014     705
 
SfcI C'TryA_G

Cuts at:     33     538     687     762    1447    2440    2631      33 
   Size:        505     149      75     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     505     191     149      75
 
SfoI GGC'GCC

Cuts at:   1151    1399    1151 
   Size:        248    3471
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3719
 
SmaI CCC'GGG

Cuts at:    741     741 
   Size:       3719
 
SmlI C'TyrA_G

Cuts at:    874    1789    2281    2543     874 
   Size:        915     492     262    2050

  Fragments arranged by size: 

                2050     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3719
 
SphI G_CATG'C

Cuts at:    772    1272     772 
   Size:        500    3219
 
StyI C'CwwG_G

Cuts at:    853    1207     853 
   Size:        354    3365
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1302    1435    1894    2103    1302 
   Size:        133     459     209    2918

  Fragments arranged by size: 

                2918     459     209     133
 
TatI w'GTAC_w

Cuts at:    220    3234     220 
   Size:       3014     705
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3236    3268    3236 
   Size:         32    3687
 
Tth111I GACn'n_nGTC

Cuts at:   1516    1516 
   Size:       3719
 
XbaI T'CTAG_A

Cuts at:    750     750 
   Size:       3719
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    141     690    1214     141 
   Size:        549     524    2646

  Fragments arranged by size: 

                2646     549     524
 
XmaI C'CCGG_G

Cuts at:    739     739 
   Size:       3719
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI    BciVI     BclI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI
   BstAPI    BstBI    BstXI    BstYI    BtgZI     BtsI    CspCI     DraI
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I
    EcoRI     FalI     FspI    HaeII    Hin4I   HincII     KasI     KpnI
     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI   NgoMIV
     NspI     PciI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     StyI    TaqII     TatI
     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI     BcgI    BfrBI     BmtI    BsaXI     BsgI
    BsiWI     BsmI    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I
     BtgI     ClaI   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII
     HpaI     MfeI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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