Restriction map for
pF1KB8232
(Circular) (Six-base) MAPSORT of: pF1KB8232.seq Check: 716 from: 1 to: 4061
pF1KB8232 4061 bp
With 182 enzymes: SgfI *
March 2, 2010 14:28 ..
AccI GT'mk_AC
Cuts at: 115 349 612 1099 115
Size: 234 263 487 3077
Fragments arranged by size:
3077 487 263 234
Acc65I G'GTAC_C
Cuts at: 1077 1077
Size: 4061
AclI AA'CG_TT
Cuts at: 3901 3901
Size: 4061
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 1886 2318 3065 3333 580
Size: 1306 432 747 268 1308
Fragments arranged by size:
1308 1306 747 432 268
AfeI AGC'GCT
Cuts at: 710 710
Size: 4061
AflIII A'CryG_T
Cuts at: 148 1413 2517 3494 148
Size: 1265 1104 977 715
Fragments arranged by size:
1265 1104 977 715
AgeI A'CCGG_T
Cuts at: 2449 2449
Size: 4061
AhdI GACnn_n'nnGTC
Cuts at: 750 750
Size: 4061
AleI CACnn'nnGTG
Cuts at: 1033 1033
Size: 4061
AlwNI CAG_nnn'CTG
Cuts at: 376 761 1366 2933 376
Size: 385 605 1567 1504
Fragments arranged by size:
1567 1504 605 385
ApaLI G'TGCA_C
Cuts at: 2831 2831
Size: 4061
ApoI r'AATT_y
Cuts at: 391 662 1021 1065 3414 3670 391
Size: 271 359 44 2349 256 782
Fragments arranged by size:
2349 782 359 271 256 44
AseI AT'TA_AT
Cuts at: 20 20
Size: 4061
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
AvaI C'yCGr_G
Cuts at: 302 989 1081 302
Size: 687 92 3282
Fragments arranged by size:
3282 687 92
AvrII C'CTAG_G
Cuts at: 628 628
Size: 4061
BamHI G'GATC_C
Cuts at: 1086 1086
Size: 4061
BanI G'GyrC_C
Cuts at: 1077 1491 1739 1774 1077
Size: 414 248 35 3364
Fragments arranged by size:
3364 414 248 35
BanII G_rGCy'C
Cuts at: 856 996 1075 2105 856
Size: 140 79 1030 2812
Fragments arranged by size:
2812 1030 140 79
BbeI G_GCGC'C
Cuts at: 1495 1743 1495
Size: 248 3813
BbsI GAAGACnn'nnnn_
Cuts at: 769 769
Size: 4061
BciVI GTATCCnnnnn_n'
Cuts at: 1959 2720 4061 1959
Size: 761 1341 1959
Fragments arranged by size:
1959 1341 761
BclI T'GATC_A
Cuts at: 1581 1581
Size: 4061
BfrBI ATG'CAT
Cuts at: 457 457
Size: 4061
BglI GCCn_nnn'nGGC
Cuts at: 895 895
Size: 4061
BglII A'GATC_T
Cuts at: 999 1576 3613 999
Size: 577 2037 1447
Fragments arranged by size:
2037 1447 577
BlpI GC'TnA_GC
Cuts at: 1173 1173
Size: 4061
Bme1580I G_kGCm'C
Cuts at: 370 1686 1779 2835 370
Size: 1316 93 1056 1596
Fragments arranged by size:
1596 1316 1056 93
BmrI ACTGGGnnnn_n'
Cuts at: 749 1027 1273 1354 1689 3833 749
Size: 278 246 81 335 2144 977
Fragments arranged by size:
2144 977 335 278 246 81
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2428 2428
Size: 4061
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1237 2116 2608 2906 1237
Size: 879 492 298 2392
Fragments arranged by size:
2392 879 492 298
BsaI GGTCTCn'nnnn_
Cuts at: 3765 3765
Size: 4061
BsaAI yAC'GTr
Cuts at: 1414 1414
Size: 4061
BsaBI GATnn'nnATC
Cuts at: 6 1575 3612 6
Size: 1569 2037 455
Fragments arranged by size:
2037 1569 455
BsaHI Gr'CG_yC
Cuts at: 112 1492 1740 2442 3938 112
Size: 1380 248 702 1496 235
Fragments arranged by size:
1496 1380 702 248 235
BsaWI w'CCGG_w
Cuts at: 1250 1771 2449 2723 2870 3281 1250
Size: 521 678 274 147 411 2030
Fragments arranged by size:
2030 678 521 411 274 147
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 864 864
Size: 4061
BseYI C'CCAG_C
Cuts at: 1486 2244 2821 3317 1486
Size: 758 577 496 2230
Fragments arranged by size:
2230 758 577 496
BsiEI CG_ry'CG
Cuts at: 104 1603 1649 2857 104
Size: 1499 46 1208 1308
Fragments arranged by size:
1499 1308 1208 46
BsiHKAI G_wGCw'C
Cuts at: 1075 1853 2043 2835 1075
Size: 778 190 792 2301
Fragments arranged by size:
2301 792 778 190
BsmI GAATG_Cn'
Cuts at: 457 459 457
Size: 2 4059
Bsp1286I G_dGCh'C
Cuts at: 370 856 996 1075 1686 1779 1853 2043 2105
Size: 486 140 79 611 93 74 190 62
Cuts at: 2105 2835 370
Size: 730 1596
Fragments arranged by size:
1596 730 611 486 190 140 93 79
74 62
BspEI T'CCGG_A
Cuts at: 3281 3281
Size: 4061
BspHI T'CATG_A
Cuts at: 3237 3237
Size: 4061
BspMI ACCTGCnnnn'nnnn_
Cuts at: 1111 1325 1627 2008 1111
Size: 214 302 381 3164
Fragments arranged by size:
3164 381 302 214
BsrBI CCG'CTC
Cuts at: 50 2353 2407 3891 4056 50
Size: 2303 54 1484 165 55
Fragments arranged by size:
2303 1484 165 55 54
BsrDI GCAATG_nn'
Cuts at: 282 452 1973 282
Size: 170 1521 2370
Fragments arranged by size:
2370 1521 170
BsrFI r'CCGG_y
Cuts at: 193 2059 2240 2449 193
Size: 1866 181 209 1805
Fragments arranged by size:
1866 1805 209 181
BssSI C'ACGA_G
Cuts at: 2332 2690 2332
Size: 358 3703
BstAPI GCAn_nnn'nTGC
Cuts at: 1438 1438
Size: 4061
BstBI TT'CG_AA
Cuts at: 2422 2422
Size: 4061
BstEII G'GTnAC_C
Cuts at: 232 232
Size: 4061
BstXI CCAn_nnnn'nTGG
Cuts at: 126 864 126
Size: 738 3323
BstYI r'GATC_y
Cuts at: 1 253 294 999 1086 1553 1576 1911 2157
Size: 252 41 705 87 467 23 335 246
Cuts at: 2157 3169 3255 3267 3613 1
Size: 1012 86 12 346 449
Fragments arranged by size:
1012 705 467 449 346 335 252 246
87 86 41 23 12
BtgI C'CryG_G
Cuts at: 107 476 107
Size: 369 3692
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 2011 2192 2355 2011
Size: 181 163 3717
Fragments arranged by size:
3717 181 163
BtsI GCAGTG_nn'
Cuts at: 1304 1304
Size: 4061
DraI TTT'AAA
Cuts at: 639 693 1060 639
Size: 54 367 3640
Fragments arranged by size:
3640 367 54
DrdI GACnn_nn'nnGTC
Cuts at: 1767 2625 1767
Size: 858 3203
EaeI y'GGCC_r
Cuts at: 1646 1820 2211 2238 3583 1646
Size: 174 391 27 1345 2124
Fragments arranged by size:
2124 1345 391 174 27
EagI C'GGCC_G
Cuts at: 1646 1646
Size: 4061
EarI CTCTTCn'nnn_
Cuts at: 140 332 547 561 2084 2294 140
Size: 192 215 14 1523 210 1907
Fragments arranged by size:
1907 1523 215 210 192 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 2269 2579 2725 3871 2269
Size: 310 146 1146 2459
Fragments arranged by size:
2459 1146 310 146
EcoICRI GAG'CTC
Cuts at: 1073 1073
Size: 4061
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 580 1886 2318 3065 3333 580
Size: 1306 432 747 268 1308
Fragments arranged by size:
1308 1306 747 432 268
EcoO109I rG'GnC_Cy
Cuts at: 914 1200 914
Size: 286 3775
EcoRI G'AATT_C
Cuts at: 1065 1065
Size: 4061
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2080 2080
Size: 4061
FspI TGC'GCA
Cuts at: 1842 1842
Size: 4061
HaeII r_GCGC'y
Cuts at: 712 1495 1743 2765 3744 712
Size: 783 248 1022 979 1029
Fragments arranged by size:
1029 1022 979 783 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 238 270 736 768 972 1004 1903 1933 1935
Size: 32 466 32 204 32 899 30 2
Cuts at: 1935 1965 238
Size: 30 2334
Fragments arranged by size:
2334 899 466 204 32 32 32 30
30 2
HincII GTy'rAC
Cuts at: 1100 1100
Size: 4061
KasI G'GCGC_C
Cuts at: 1491 1739 1491
Size: 248 3813
KpnI G_GTAC'C
Cuts at: 1081 1081
Size: 4061
MluI A'CGCG_T
Cuts at: 3494 3494
Size: 4061
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 288 791 2732 2916 288
Size: 503 1941 184 1433
Fragments arranged by size:
1941 1433 503 184
MscI TGG'CCA
Cuts at: 1822 1822
Size: 4061
MslI CAynn'nnrTG
Cuts at: 164 475 862 1033 2050 2177 164
Size: 311 387 171 1017 127 2048
Fragments arranged by size:
2048 1017 387 311 171 127
MspA1I CmG'CkG
Cuts at: 871 904 1172 1486 1846 2859 3104 871
Size: 33 268 314 360 1013 245 1828
Fragments arranged by size:
1828 1013 360 314 268 245 33
NaeI GCC'GGC
Cuts at: 2242 2242
Size: 4061
NarI GG'CG_CC
Cuts at: 1492 1740 1492
Size: 248 3813
NcoI C'CATG_G
Cuts at: 107 476 107
Size: 369 3692
NgoMIV G'CCGG_C
Cuts at: 2240 2240
Size: 4061
NsiI A_TGCA'T
Cuts at: 459 459
Size: 4061
NspI r_CATG'y
Cuts at: 152 1114 1614 2521 152
Size: 962 500 907 1692
Fragments arranged by size:
1692 962 907 500
PciI A'CATG_T
Cuts at: 148 2517 148
Size: 2369 1692
PmeI GTTT'AAAC
Cuts at: 1060 1060
Size: 4061
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3200 3232 3200
Size: 32 4029
PpuMI rG'GwC_Cy
Cuts at: 914 914
Size: 4061
PspXI vC'TCGA_Gb
Cuts at: 989 989
Size: 4061
PstI C_TGCA'G
Cuts at: 1108 1793 1108
Size: 685 3376
PvuI CG_AT'CG
Cuts at: 104 104
Size: 4061
PvuII CAG'CTG
Cuts at: 904 1486 1846 904
Size: 582 360 3119
Fragments arranged by size:
3119 582 360
RsrII CG'GwC_CG
Cuts at: 2256 2256
Size: 4061
SacI G_AGCT'C
Cuts at: 1075 1075
Size: 4061
SalI G'TCGA_C
Cuts at: 1098 1098
Size: 4061
SapI GCTCTTCn'nnn_
Cuts at: 140 547 561 2084 2294 140
Size: 407 14 1523 210 1907
Fragments arranged by size:
1907 1523 407 210 14
SbfI CC_TGCA'GG
Cuts at: 1108 1108
Size: 4061
ScaI AGT'ACT
Cuts at: 3578 3578
Size: 4061
SfcI C'TryA_G
Cuts at: 33 186 581 609 972 1010 1104 1789 2782
Size: 153 395 28 363 38 94 685 993
Cuts at: 2782 2973 33
Size: 191 1121
Fragments arranged by size:
1121 993 685 395 363 191 153 94
38 28
SfoI GGC'GCC
Cuts at: 1493 1741 1493
Size: 248 3813
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 4061
SmaI CCC'GGG
Cuts at: 1083 1083
Size: 4061
SmlI C'TyrA_G
Cuts at: 302 989 1216 2131 2623 2885 302
Size: 687 227 915 492 262 1478
Fragments arranged by size:
1478 915 687 492 262 227
SpeI A'CTAG_T
Cuts at: 67 67
Size: 4061
SphI G_CATG'C
Cuts at: 1114 1614 1114
Size: 500 3561
StyI C'CwwG_G
Cuts at: 107 476 628 1195 1549 107
Size: 369 152 567 354 2619
Fragments arranged by size:
2619 567 369 354 152
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 1644 1777 2236 2445 1644
Size: 133 459 209 3260
Fragments arranged by size:
3260 459 209 133
TatI w'GTAC_w
Cuts at: 789 3576 789
Size: 2787 1274
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3578 3610 3578
Size: 32 4029
Tth111I GACn'n_nGTC
Cuts at: 1858 1858
Size: 4061
XbaI T'CTAG_A
Cuts at: 1092 1092
Size: 4061
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 925 1556 925
Size: 631 3430
XhoI C'TCGA_G
Cuts at: 302 989 302
Size: 687 3374
XmaI C'CCGG_G
Cuts at: 1081 1081
Size: 4061
XmnI GAAnn'nnTTC
Cuts at: 457 623 457
Size: 166 3895
Enzymes that do cut:
AccI Acc65I AclI AcuI AfeI AflIII AgeI AhdI
AleI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmrI BplI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI
BtsI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NsiI NspI PciI PmeI PpiI
PpuMI PspXI PstI PvuI PvuII RsrII SacI SalI
SapI SbfI ScaI SfcI SfoI SgfI SmaI SmlI
SpeI SphI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AflII AlfI AloI ApaI AscI BaeI
BbvCI BcgI BmgBI BmtI BpmI Bpu10I BsaXI BsgI
BsiWI BsmBI BsrGI BssHII BstZ17I Bsu36I ClaI CspCI
DraIII EcoNI EcoRV FseI FspAI HindIII HpaI MfeI
NdeI NheI NotI NruI PacI PasI PflMI PfoI
PmlI PshAI PsiI PspOMI PsrI SacII SanDI SexAI
SfiI SgrAI SnaBI SrfI SspI StuI SwaI ZraI