Restriction map for pF1KB8232
 (Linear) (Six-base) MAPSORT of: KIBB8232.seq  Check: 257  from: 1  to: 948



KIBB8232.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AccI GT'mk_AC

Cuts at:      0       7     241     504     948 
   Size:          7     234     263     444

  Fragments arranged by size: 

                 444     263     234       7
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     948 
   Size:        472     476
 
AfeI AGC'GCT

Cuts at:      0     602     948 
   Size:        602     346
 
AflIII A'CryG_T

Cuts at:      0      40     948 
   Size:         40     908
 
AhdI GACnn_n'nnGTC

Cuts at:      0     642     948 
   Size:        642     306
 
AleI CACnn'nnGTG

Cuts at:      0     925     948 
   Size:        925      23
 
AlwNI CAG_nnn'CTG

Cuts at:      0     268     653     948 
   Size:        268     385     295

  Fragments arranged by size: 

                 385     295     268
 
ApoI r'AATT_y

Cuts at:      0     283     554     913     948 
   Size:        283     271     359      35

  Fragments arranged by size: 

                 359     283     271      35
 
AvaI C'yCGr_G

Cuts at:      0     194     881     948 
   Size:        194     687      67

  Fragments arranged by size: 

                 687     194      67
 
AvrII C'CTAG_G

Cuts at:      0     520     948 
   Size:        520     428
 
BanII G_rGCy'C

Cuts at:      0     748     888     948 
   Size:        748     140      60

  Fragments arranged by size: 

                 748     140      60
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     661     948 
   Size:        661     287
 
BfrBI ATG'CAT

Cuts at:      0     349     948 
   Size:        349     599
 
BglI GCCn_nnn'nGGC

Cuts at:      0     787     948 
   Size:        787     161
 
BglII A'GATC_T

Cuts at:      0     891     948 
   Size:        891      57
 
Bme1580I G_kGCm'C

Cuts at:      0     262     948 
   Size:        262     686
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     641     919     948 
   Size:        641     278      29

  Fragments arranged by size: 

                 641     278      29
 
BsaHI Gr'CG_yC

Cuts at:      0       4     948 
   Size:          4     944
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     756     948 
   Size:        756     192
 
BsmI GAATG_Cn'

Cuts at:      0     349     351     948 
   Size:        349       2     597

  Fragments arranged by size: 

                 597     349       2
 
Bsp1286I G_dGCh'C

Cuts at:      0     262     748     888     948 
   Size:        262     486     140      60

  Fragments arranged by size: 

                 486     262     140      60
 
BsrDI GCAATG_nn'

Cuts at:      0     174     344     948 
   Size:        174     170     604

  Fragments arranged by size: 

                 604     174     170
 
BsrFI r'CCGG_y

Cuts at:      0      85     948 
   Size:         85     863
 
BstEII G'GTnAC_C

Cuts at:      0     124     948 
   Size:        124     824
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      18     756     948 
   Size:         18     738     192

  Fragments arranged by size: 

                 738     192      18
 
BstYI r'GATC_y

Cuts at:      0     145     186     891     948 
   Size:        145      41     705      57

  Fragments arranged by size: 

                 705     145      57      41
 
BtgI C'CryG_G

Cuts at:      0     368     948 
   Size:        368     580
 
DraI TTT'AAA

Cuts at:      0     531     585     948 
   Size:        531      54     363

  Fragments arranged by size: 

                 531     363      54
 
EarI CTCTTCn'nnn_

Cuts at:      0      32     224     439     453     948 
   Size:         32     192     215      14     495

  Fragments arranged by size: 

                 495     215     192      32      14
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     472     948 
   Size:        472     476
 
EcoO109I rG'GnC_Cy

Cuts at:      0     806     948 
   Size:        806     142
 
HaeII r_GCGC'y

Cuts at:      0     604     948 
   Size:        604     344
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     130     162     628     660     864     896     948 
   Size:        130      32     466      32     204      32      52

  Fragments arranged by size: 

                 466     204     130      52      32      32      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     180     683     948 
   Size:        180     503     265

  Fragments arranged by size: 

                 503     265     180
 
MslI CAynn'nnrTG

Cuts at:      0      56     367     754     925     948 
   Size:         56     311     387     171      23

  Fragments arranged by size: 

                 387     311     171      56      23
 
MspA1I CmG'CkG

Cuts at:      0     763     796     948 
   Size:        763      33     152

  Fragments arranged by size: 

                 763     152      33
 
NcoI C'CATG_G

Cuts at:      0     368     948 
   Size:        368     580
 
NsiI A_TGCA'T

Cuts at:      0     351     948 
   Size:        351     597
 
NspI r_CATG'y

Cuts at:      0      44     948 
   Size:         44     904
 
PciI A'CATG_T

Cuts at:      0      40     948 
   Size:         40     908
 
PpuMI rG'GwC_Cy

Cuts at:      0     806     948 
   Size:        806     142
 
PspXI vC'TCGA_Gb

Cuts at:      0     881     948 
   Size:        881      67
 
PvuII CAG'CTG

Cuts at:      0     796     948 
   Size:        796     152
 
SapI GCTCTTCn'nnn_

Cuts at:      0      32     439     453     948 
   Size:         32     407      14     495

  Fragments arranged by size: 

                 495     407      32      14
 
SfcI C'TryA_G

Cuts at:      0      78     473     501     864     902     948 
   Size:         78     395      28     363      38      46

  Fragments arranged by size: 

                 395     363      78      46      38      28
 
SmlI C'TyrA_G

Cuts at:      0     194     881     948 
   Size:        194     687      67

  Fragments arranged by size: 

                 687     194      67
 
StyI C'CwwG_G

Cuts at:      0     368     520     948 
   Size:        368     152     428

  Fragments arranged by size: 

                 428     368     152
 
TatI w'GTAC_w

Cuts at:      0     681     948 
   Size:        681     267
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     817     948 
   Size:        817     131
 
XhoI C'TCGA_G

Cuts at:      0     194     881     948 
   Size:        194     687      67

  Fragments arranged by size: 

                 687     194      67
 
XmnI GAAnn'nnTTC

Cuts at:      0     349     515     948 
   Size:        349     166     433

  Fragments arranged by size: 

                 433     349     166
 

 Enzymes that do cut:

     AccI     AcuI     AfeI   AflIII     AhdI     AleI    AlwNI     ApoI
     AvaI    AvrII    BanII     BbsI    BfrBI     BglI    BglII Bme1580I
     BmrI    BsaHI    BseRI     BsmI Bsp1286I    BsrDI    BsrFI   BstEII
    BstXI    BstYI     BtgI     DraI     EarI  Eco57MI EcoO109I    HaeII
    Hin4I     MmeI     MslI   MspA1I     NcoI     NsiI     NspI     PciI
    PpuMI    PspXI    PvuII     SapI     SfcI     SmlI     StyI     TatI
     XcmI     XhoI     XmnI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI    AflII     AgeI     AlfI     AloI
     ApaI    ApaLI     AscI     AseI    AsiSI     BaeI    BamHI     BanI
     BbeI    BbvCI     BcgI    BciVI     BclI     BlpI    BmgBI     BmtI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrGI   BssHII    BssSI   BstAPI    BstBI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NdeI   NgoMIV     NheI
     NotI     NruI     PacI     PasI    PflMI     PfoI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XmaI
     ZraI
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