Restriction map for pF1KB6749
##################################
# NO CUT ENZYMES (  178 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AflII       AflIII      AgeI        AhdI        AluI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanII       
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaI        BseAI       BsgI        BsiEI       BsiWI       
BsiYI       BslI        BsmBI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrGI       BsrI        
BssHII      Bst1107I    BstAPI      BstBI       BstEII      
BstNI       BstPI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr13I      ClaI        CpoI        DdeI        
DraI        DraII       DraIII      DrdI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT22I     FbaI        FseI        
FspI        HincII      HindII      HindIII     HpaI        
KpnI        Ksp632I     KspI        MamI        MboII       
MfeI        MflI        MluI        MluNI       MroI        
MscI        MspA1I      MunI        MvaI        NaeI        
NdeI        NgoMIV      NheI        NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfuI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tth111I     TthHB8I     
Van91I      XbaI        XcmI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AcsI          137  GAATTT               [GA]^AATT[CT]
AfaI          294  GTAC                 GT^AC
AlwI          198  GATCC                GGATC(4/5)
ApoI          137  GAATTT               [GA]^AATT[CT]
AspHI         223  GAGCAC               G[AT]GC[AT]^C
BbeI          107  GGCGCC               GGCGC^C
BbvI           66  GCTGC                GCAGC(8/12)
BcnI          201  CCGGG                CC^[GC]GG
BfaI          116  CTAG                 C^TAG
BmyI          223  GAGCAC               G[AGT]GC[ACT]^C
BpmI          187  CTGGAG               CTGGAG(16/14)
BsaWI         142  TCCGGT               [AT]^CCGG[AT]
BseRI         157  GAGGAG               GAGGAG(10/8)
BsiHKAI       223  GAGCAC               G[AT]GC[AT]^C
BsmAI         160  GAGAC                GTCTC(1/5)
BsmFI         203  GGGAC                GGGAC(10/14)
Bsp1286I      223  GAGCAC               G[AGT]GC[ACT]^C
BsrFI          96  ACCGGC               [GA]^CCGG[CT]
BssKI         201  CCGGG                ^CC[ACGT]GG
BssSI         164  CTCGTG               CACGAG(-5/-1)
BstXI         251  CCATGGATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I         225  GCACGC               GC[ACGT]^[ACGT]GC
Cfr10I         96  ACCGGC               [GA]^CCGG[CT]
DpnII         198  GATC                 ^GATC
DsaI          251  CCATGG               C^C[GA][CT]GG
EaeI           98  CGGCCA               [CT]^GGCC[GA]
FauI           69  GCGGG                CCCGC(4/6)
HaeII         107  GGCGCC               [GA]GCGC^[CT]
HaeIII         99  GGCC                 GG^CC
HinfI         162  GACTC                G^A[ACGT]TC
KasI          107  GGCGCC               G^GCGCC
MaeI          116  CTAG                 C^TAG
MaeII         194  ACGT                 A^CGT
MboI          198  GATC                 ^GATC
MseI          131  TTAA                 T^TAA
NarI          107  GGCGCC               GG^CGCC
NciI          201  CCGGG                CC^[GC]GG
NcoI          251  CCATGG               C^CATGG
NdeII         198  GATC                 ^GATC
NlaIII        252  CATG                 CATG^
PleI          162  GACTC                GAGTC(4/5)
RsaI          294  GTAC                 GT^AC
Sau3AI        198  GATC                 ^GATC
ScrFI         201  CCGGG                CC^[ACGT]GG
SfaNI          86  GCATC                GCATC(5/9)
SfoI          107  GGCGCC               GGC^GCC
Tru9I         131  TTAA                 T^TAA
TseI           66  GCTGC                G^C[AT]GC
Tsp509I       138  AATT                 ^AATT
TspRI         246  CACTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AccII          10  CGCG                 CG^CG
              267  CGCG                
AcyI          107  GGCGCC               G[GA]^CG[CT]C
              205  GACGCC              
BanI           43  GGTGCC               G^G[CT][GA]CC
              107  GGCGCC              
BsaHI         107  GGCGCC               G[GA]^CG[CT]C
              205  GACGCC              
BsaJI          81  CCAAGG               C^C[ACGT][ACGT]GG
              251  CCATGG              
BstF5I         78  CATCC                GGATG(2/0)
              255  GGATG               
BstUI          10  CGCG                 CG^CG
              267  CGCG                
EcoT14I        81  CCAAGG               C^C[AT][AT]GG
              251  CCATGG              
Fnu4HI         11  GCGGC                GC^[ACGT]GC
               66  GCTGC               
FokI           78  CATCC                GGATG(9/13)
              255  GGATG               
Hin1I         107  GGCGCC               G[GA]^CG[CT]C
              205  GACGCC              
HphI           54  TCACC                GGTGA(8/7)
               89  TCACC               
ItaI           11  GCGGC                GC^[ACGT]GC
               66  GCTGC               
MnlI           41  GAGG                 CCTC(7/6)
              157  GAGG                
MslI          246  CACTGCCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              252  CATGGATGTG          
MvnI           10  CGCG                 CG^CG
              267  CGCG                
MwoI           11  GCGGCAAAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              225  GCACGCCAAGC         
NlaIV          43  GGTGCC               GG[ACGT]^[ACGT]CC
              107  GGCGCC              
SmlI          175  CTCAAG               C^T[CT][GA]AG
              271  CTCAAG              
StyI           81  CCAAGG               C^C[AT][AT]GG
              251  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   10 enzymes) #
#########################################

AciI        CfoI        HapII       HgaI        HhaI        
HinP1I      HpaII       MaeIII      MspI        Tsp45I      
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