Restriction map for pF1KB6749
 (Circular) (Six-base) MAPSORT of: pF1KB6749.seq  Check: 9543  from: 1  to: 3422



pF1KB6749  3422 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AccI GT'mk_AC

Cuts at:    460     460 
   Size:       3422
 
Acc65I G'GTAC_C

Cuts at:    438     438 
   Size:       3422
 
AclI AA'CG_TT

Cuts at:   3262    3262 
   Size:       3422
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1247    1679    2426    2694    1247 
   Size:        432     747     268    1975

  Fragments arranged by size: 

                1975     747     432     268
 
AflIII A'CryG_T

Cuts at:    774    1878    2855     774 
   Size:       1104     977    1341

  Fragments arranged by size: 

                1341    1104     977
 
AgeI A'CCGG_T

Cuts at:   1810    1810 
   Size:       3422
 
AlwNI CAG_nnn'CTG

Cuts at:    727    2294     727 
   Size:       1567    1855
 
ApaLI G'TGCA_C

Cuts at:   2192    2192 
   Size:       3422
 
ApoI r'AATT_y

Cuts at:    245     426    2775    3031     245 
   Size:        181    2349     256     636

  Fragments arranged by size: 

                2349     636     256     181
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3422
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3422
 
AvaI C'yCGr_G

Cuts at:    442     442 
   Size:       3422
 
BamHI G'GATC_C

Cuts at:    447     447 
   Size:       3422
 
BanI G'GyrC_C

Cuts at:    151     215     438     852    1100    1135     151 
   Size:         64     223     414     248      35    2438

  Fragments arranged by size: 

                2438     414     248     223      64      35
 
BanII G_rGCy'C

Cuts at:    436    1466     436 
   Size:       1030    2392
 
BbeI G_GCGC'C

Cuts at:    219     856    1104     219 
   Size:        637     248    2537

  Fragments arranged by size: 

                2537     637     248
 
BciVI GTATCCnnnnn_n'

Cuts at:   1320    2081    3422    1320 
   Size:        761    1341    1320

  Fragments arranged by size: 

                1341    1320     761
 
BclI T'GATC_A

Cuts at:    942     942 
   Size:       3422
 
BglII A'GATC_T

Cuts at:    937    2974     937 
   Size:       2037    1385
 
BlpI GC'TnA_GC

Cuts at:    534     534 
   Size:       3422
 
Bme1580I G_kGCm'C

Cuts at:   1047    1140    2196    1047 
   Size:         93    1056    2273

  Fragments arranged by size: 

                2273    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    634     715    1050    3194     634 
   Size:         81     335    2144     862

  Fragments arranged by size: 

                2144     862     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1789    1789 
   Size:       3422
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    316     316 
   Size:       3422
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    268     364     598    1477    1969    2267     268 
   Size:         96     234     879     492     298    1423

  Fragments arranged by size: 

                1423     879     492     298     234      96
 
BsaI GGTCTCn'nnnn_

Cuts at:   3126    3126 
   Size:       3422
 
BsaAI yAC'GTr

Cuts at:    775     775 
   Size:       3422
 
BsaBI GATnn'nnATC

Cuts at:      6     936    2973       6 
   Size:        930    2037     455

  Fragments arranged by size: 

                2037     930     455
 
BsaHI Gr'CG_yC

Cuts at:    216     314     853    1101    1803    3299     216 
   Size:         98     539     248     702    1496     339

  Fragments arranged by size: 

                1496     702     539     339     248      98
 
BsaWI w'CCGG_w

Cuts at:    250     611    1132    1810    2084    2231    2642     250 
   Size:        361     521     678     274     147     411    1030

  Fragments arranged by size: 

                1030     678     521     411     361     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    280     280 
   Size:       3422
 
BseYI C'CCAG_C

Cuts at:    847    1605    2182    2678     847 
   Size:        758     577     496    1591

  Fragments arranged by size: 

                1591     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104     964    1010    2218     104 
   Size:        860      46    1208    1308

  Fragments arranged by size: 

                1308    1208     860      46
 
BsiHKAI G_wGCw'C

Cuts at:    335     436    1214    1404    2196     335 
   Size:        101     778     190     792    1561

  Fragments arranged by size: 

                1561     792     778     190     101
 
Bsp1286I G_dGCh'C

Cuts at:    335     436    1047    1140    1214    1404    1466    2196     335 
   Size:        101     611      93      74     190      62     730    1561

  Fragments arranged by size: 

                1561     730     611     190     101      93      74      62
            
 
BspEI T'CCGG_A

Cuts at:   2642    2642 
   Size:       3422
 
BspHI T'CATG_A

Cuts at:   2598    2598 
   Size:       3422
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    472     686     988    1369     472 
   Size:        214     302     381    2525

  Fragments arranged by size: 

                2525     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1714    1768    3252    3417      50 
   Size:       1664      54    1484     165      55

  Fragments arranged by size: 

                1664    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1334    1334 
   Size:       3422
 
BsrFI r'CCGG_y

Cuts at:    204    1420    1601    1810     204 
   Size:       1216     181     209    1816

  Fragments arranged by size: 

                1816    1216     209     181
 
BssSI C'ACGA_G

Cuts at:    272    1693    2051     272 
   Size:       1421     358    1643

  Fragments arranged by size: 

                1643    1421     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    799     799 
   Size:       3422
 
BstBI TT'CG_AA

Cuts at:   1783    1783 
   Size:       3422
 
BstXI CCAn_nnnn'nTGG

Cuts at:    366     366 
   Size:       3422
 
BstYI r'GATC_y

Cuts at:      1     447     914     937    1272    1518    2530    2616    2628 
   Size:        446     467      23     335     246    1012      86      12

Cuts at:   2628    2974       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     467     449     446     346     335     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    359     359 
   Size:       3422
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1372    1553    1716    1372 
   Size:        181     163    3078

  Fragments arranged by size: 

                3078     181     163
 
BtsI GCAGTG_nn'

Cuts at:    353     665     353 
   Size:        312    3110
 
DraI TTT'AAA

Cuts at:    421     421 
   Size:       3422
 
DrdI GACnn_nn'nnGTC

Cuts at:   1128    1986    1128 
   Size:        858    2564
 
EaeI y'GGCC_r

Cuts at:    206    1007    1181    1572    1599    2944     206 
   Size:        801     174     391      27    1345     684

  Fragments arranged by size: 

                1345     801     684     391     174      27
 
EagI C'GGCC_G

Cuts at:   1007    1007 
   Size:       3422
 
EarI CTCTTCn'nnn_

Cuts at:   1445    1655    1445 
   Size:        210    3212
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1630    1940    2086    3232    1630 
   Size:        310     146    1146    1820

  Fragments arranged by size: 

                1820    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    434     434 
   Size:       3422
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    316    1247    1679    2426    2694     316 
   Size:        931     432     747     268    1044

  Fragments arranged by size: 

                1044     931     747     432     268
 
EcoO109I rG'GnC_Cy

Cuts at:    561     561 
   Size:       3422
 
EcoRI G'AATT_C

Cuts at:    426     426 
   Size:       3422
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1441    1441 
   Size:       3422
 
FspI TGC'GCA

Cuts at:   1203    1203 
   Size:       3422
 
HaeII r_GCGC'y

Cuts at:    219     856    1104    2126    3105     219 
   Size:        637     248    1022     979     536

  Fragments arranged by size: 

                1022     979     637     536     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1264    1294    1296    1326    1264 
   Size:         30       2      30    3360

  Fragments arranged by size: 

                3360      30      30       2
 
HincII GTy'rAC

Cuts at:    461     461 
   Size:       3422
 
KasI G'GCGC_C

Cuts at:    215     852    1100     215 
   Size:        637     248    2537

  Fragments arranged by size: 

                2537     637     248
 
KpnI G_GTAC'C

Cuts at:    442     442 
   Size:       3422
 
MluI A'CGCG_T

Cuts at:   2855    2855 
   Size:       3422
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   2093    2277    2093 
   Size:        184    3238
 
MscI TGG'CCA

Cuts at:   1183    1183 
   Size:       3422
 
MslI CAynn'nnrTG

Cuts at:    358     364    1411    1538     358 
   Size:          6    1047     127    2242

  Fragments arranged by size: 

                2242    1047     127       6
 
MspA1I CmG'CkG

Cuts at:    533     847    1207    2220    2465     533 
   Size:        314     360    1013     245    1490

  Fragments arranged by size: 

                1490    1013     360     314     245
 
NaeI GCC'GGC

Cuts at:   1603    1603 
   Size:       3422
 
NarI GG'CG_CC

Cuts at:    216     853    1101     216 
   Size:        637     248    2537

  Fragments arranged by size: 

                2537     637     248
 
NcoI C'CATG_G

Cuts at:    359     359 
   Size:       3422
 
NgoMIV G'CCGG_C

Cuts at:   1601    1601 
   Size:       3422
 
NspI r_CATG'y

Cuts at:    475     975    1882     475 
   Size:        500     907    2015

  Fragments arranged by size: 

                2015     907     500
 
PciI A'CATG_T

Cuts at:   1878    1878 
   Size:       3422
 
PfoI T'CCnGG_A

Cuts at:    308     308 
   Size:       3422
 
PmeI GTTT'AAAC

Cuts at:    421     421 
   Size:       3422
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2561    2593    2561 
   Size:         32    3390
 
PstI C_TGCA'G

Cuts at:    469    1154     469 
   Size:        685    2737
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3422
 
PvuII CAG'CTG

Cuts at:    847    1207     847 
   Size:        360    3062
 
RsrII CG'GwC_CG

Cuts at:   1617    1617 
   Size:       3422
 
SacI G_AGCT'C

Cuts at:    436     436 
   Size:       3422
 
SalI G'TCGA_C

Cuts at:    459     459 
   Size:       3422
 
SapI GCTCTTCn'nnn_

Cuts at:   1445    1655    1445 
   Size:        210    3212
 
SbfI CC_TGCA'GG

Cuts at:    469     469 
   Size:       3422
 
ScaI AGT'ACT

Cuts at:   2939    2939 
   Size:       3422
 
SfcI C'TryA_G

Cuts at:     33     465    1150    2143    2334      33 
   Size:        432     685     993     191    1121

  Fragments arranged by size: 

                1121     993     685     432     191
 
SfoI GGC'GCC

Cuts at:    217     854    1102     217 
   Size:        637     248    2537

  Fragments arranged by size: 

                2537     637     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3422
 
SmaI CCC'GGG

Cuts at:    444     444 
   Size:       3422
 
SmlI C'TyrA_G

Cuts at:    283     379     577    1492    1984    2246     283 
   Size:         96     198     915     492     262    1459

  Fragments arranged by size: 

                1459     915     492     262     198      96
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3422
 
SphI G_CATG'C

Cuts at:    475     975     475 
   Size:        500    2922
 
StyI C'CwwG_G

Cuts at:    189     359     556     910     189 
   Size:        170     197     354    2701

  Fragments arranged by size: 

                2701     354     197     170
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1005    1138    1597    1806    1005 
   Size:        133     459     209    2621

  Fragments arranged by size: 

                2621     459     209     133
 
TatI w'GTAC_w

Cuts at:   2937    2937 
   Size:       3422
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   2939    2971    2939 
   Size:         32    3390
 
Tth111I GACn'n_nGTC

Cuts at:   1219    1219 
   Size:       3422
 
XbaI T'CTAG_A

Cuts at:    453     453 
   Size:       3422
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    917     917 
   Size:       3422
 
XmaI C'CCGG_G

Cuts at:    442     442 
   Size:       3422
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
    BciVI     BclI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI
    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI     PfoI     PmeI     PpiI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BbsI    BbvCI     BcgI    BfrBI
     BglI    BmgBI     BmtI   Bpu10I    BsaXI     BsgI    BsiWI     BsmI
    BsmBI    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI
     PmlI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI
     SwaI     XhoI     XmnI     ZraI
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