Restriction map for
pF1KB6539
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# NO CUT ENZYMES ( 141 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BclI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstZ17I
CelII Cfr10I ClaI CpoI DraI
DraIII DsaI EagI Eam1105I EclXI
Eco47III Eco52I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI HaeII HindIII
HpaI KasI KpnI KspI MfeI
MluI MluNI MroI MscI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcyI 190 GACGCC G[GA]^CG[CT]C
AlwNI 771 CAGTGACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 294 CCTCAGG CC^T[ACGT]AGG
AviII 263 TGCGCA TGC^GCA
BciVI 162 GGATAC GTATCC(6/5)
BcnI 542 CCGGG CC^[GC]GG
BfaI 760 CTAG C^TAG
BpmI 393 CTGGAG CTGGAG(16/14)
BsaAI 891 TACGTG [CT]AC^GT[GA]
BsaBI 279 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 190 GACGCC G[GA]^CG[CT]C
BsaWI 377 ACCGGA [AT]^CCGG[AT]
BspHI 303 TCATGA T^CATGA
BspMI 226 ACCTGC ACCTGC(4/8)
BssSI 427 CACGAG CACGAG(-5/-1)
BstEII 993 GGTGACC G^GT[ACGT]ACC
BstPI 993 GGTGACC G^GT[ACGT]ACC
BstXI 959 CCAACATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 278 AGATCC [GA]^GATC[CT]
Bsu36I 294 CCTCAGG CC^T[ACGT]AGG
EaeI 735 CGGCCA [CT]^GGCC[GA]
Eco81I 294 CCTCAGG CC^T[ACGT]AGG
EcoNI 999 CCTGTGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 993 GGTGACC G^GT[ACGT]ACC
FspI 263 TGCGCA TGC^GCA
HgaI 190 GACGC GACGC(5/10)
Hin1I 190 GACGCC G[GA]^CG[CT]C
HincII 1045 GTCAAC GT[CT]^[GA]AC
HindII 1045 GTCAAC GT[CT]^[GA]AC
MaeI 760 CTAG C^TAG
MaeII 892 ACGT A^CGT
MamI 279 GATCCTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 278 AGATCC [GA]^GATC[CT]
MseI 152 TTAA T^TAA
MslI 427 CACGAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 542 CCGGG CC^[GC]GG
PpuMI 309 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 303 TCATGA T^CATGA
SacI 507 GAGCTC GAGCT^C
SapI 420 GAAGAGC GCTCTTC(1/4)
SfcI 102 CTACAG C^T[GA][CT]AG
SmlI 882 CTCAAG C^T[CT][GA]AG
SphI 432 GCATGC GCATG^C
TaqI 783 TCGA T^CGA
TfiI 183 GATTC G^A[AT]TC
Tru9I 152 TTAA T^TAA
TthHB8I 783 TCGA T^CGA
XhoII 278 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 216 CCGC CCGC(-3/-1)
436 GCGG
AlwI 279 GATCC GGATC(4/5)
492 GATCC
AspHI 366 GTGCTC G[AT]GC[AT]^C
507 GAGCTC
BanI 211 GGCACC G^G[CT][GA]CC
409 GGCACC
BsiHKAI 366 GTGCTC G[AT]GC[AT]^C
507 GAGCTC
BsmAI 983 GAGAC GTCTC(1/5)
973 GTCTC
BsmFI 438 GGGAC GGGAC(10/14)
897 GTCCC
BsrDI 266 GCAATG GCAATG(2/0)
596 GCAATG
BsrI 776 ACTGG ACTGG(1/-1)
738 CCAGT
CfoI 264 GCGC GCG^C
766 GCGC
DdeI 295 CTCAG C^T[ACGT]AG
450 CTCAG
DrdI 238 GACAGGTCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
376 GACCGGAAAGTC
EarI 56 CTCTTC CTCTTC(1/4)
420 GAAGAG
FauI 215 CCCGC CCCGC(4/6)
436 GCGGG
HapII 378 CCGG C^CGG
542 CCGG
HhaI 264 GCGC GCG^C
766 GCGC
HinP1I 264 GCGC G^CGC
766 GCGC
HpaII 378 CCGG C^CGG
542 CCGG
HphI 993 GGTGA GGTGA(8/7)
336 TCACC
Ksp632I 56 CTCTTC CTCTTC(1/4)
420 GAAGAG
MspA1I 606 CAGCTG C[AC]G^C[GT]G
834 CAGCTG
MspI 378 CCGG C^CGG
542 CCGG
MwoI 759 GCTAGAAGCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1092 GCAGAACGAGC
NspI 432 GCATGC [GA]CATG^[CT]
936 GCATGT
PleI 445 GACTC GAGTC(4/5)
1009 GAGTC
PvuII 606 CAGCTG CAG^CTG
834 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AfaI AluI ApaI AvaII BanII
BbvI BmyI BsaJI BseRI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I Cfr13I DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
ItaI MaeIII MboI MboII MnlI
MvaI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI TseI
Tsp45I Tsp509I TspRI