Restriction map for pF1KB6422
 (Linear) (Six-base) MAPSORT of: KIBB6422.seq  Check: 8916  from: 1  to: 1017



KIBB6422.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     436    1017 
   Size:        436     581
 
AccI GT'mk_AC

Cuts at:      0     662    1017 
   Size:        662     355
 
Acc65I G'GTAC_C

Cuts at:      0     490    1017 
   Size:        490     527
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     983     989    1017 
   Size:         23     960       6      28

  Fragments arranged by size: 

                 960      28      23       6
 
AlwNI CAG_nnn'CTG

Cuts at:      0     523     932    1017 
   Size:        523     409      85

  Fragments arranged by size: 

                 523     409      85
 
ApoI r'AATT_y

Cuts at:      0     218     271     382    1017 
   Size:        218      53     111     635

  Fragments arranged by size: 

                 635     218     111      53
 
AseI AT'TA_AT

Cuts at:      0     986    1017 
   Size:        986      31
 
AvaI C'yCGr_G

Cuts at:      0     575     696    1017 
   Size:        575     121     321

  Fragments arranged by size: 

                 575     321     121
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0      26      59    1017 
   Size:         26      33     958

  Fragments arranged by size: 

                 958      33      26
 
BanI G'GyrC_C

Cuts at:      0     490    1017 
   Size:        490     527
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     403     949    1017 
   Size:        403     546      68

  Fragments arranged by size: 

                 546     403      68
 
BbvCI CC'TCA_GC

Cuts at:      0     227    1017 
   Size:        227     790
 
BfrBI ATG'CAT

Cuts at:      0     883    1017 
   Size:        883     134
 
BglI GCCn_nnn'nGGC

Cuts at:      0     507    1017 
   Size:        507     510
 
BglII A'GATC_T

Cuts at:      0     404    1017 
   Size:        404     613
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     401    1017 
   Size:        401     616
 
BmtI G_CTAG'C

Cuts at:      0     421    1017 
   Size:        421     596
 
Bpu10I CC'TnA_GC

Cuts at:      0     227    1017 
   Size:        227     790
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     797    1017 
   Size:        797     220
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     541    1017 
   Size:        541     476
 
BseYI C'CCAG_C

Cuts at:      0     602    1017 
   Size:        602     415
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     436    1017 
   Size:        436     581
 
BsrDI GCAATG_nn'

Cuts at:      0      11    1017 
   Size:         11    1006
 
BsrGI T'GTAC_A

Cuts at:      0     473    1017 
   Size:        473     544
 
BstYI r'GATC_y

Cuts at:      0     358     404    1017 
   Size:        358      46     613

  Fragments arranged by size: 

                 613     358      46
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     844     858    1017 
   Size:        844      14     159

  Fragments arranged by size: 

                 844     159      14
 
BtsI GCAGTG_nn'

Cuts at:      0      94     260     524     552     649     791    1017 
   Size:         94     166     264      28      97     142     226

  Fragments arranged by size: 

                 264     226     166     142      97      94      28
 
EaeI y'GGCC_r

Cuts at:      0     734    1017 
   Size:        734     283
 
EarI CTCTTCn'nnn_

Cuts at:      0     909    1017 
   Size:        909     108
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      23     983     989    1017 
   Size:         23     960       6      28

  Fragments arranged by size: 

                 960      28      23       6
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     860    1017 
   Size:        860     157
 
EcoRI G'AATT_C

Cuts at:      0     271     382    1017 
   Size:        271     111     635

  Fragments arranged by size: 

                 635     271     111
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     343     375     519     551     854     886    1017 
   Size:        343      32     144      32     303      32     131

  Fragments arranged by size: 

                 343     303     144     131      32      32      32
 
HindIII A'AGCT_T

Cuts at:      0      54    1017 
   Size:         54     963
 
KpnI G_GTAC'C

Cuts at:      0     494    1017 
   Size:        494     523
 
MscI TGG'CCA

Cuts at:      0     736    1017 
   Size:        736     281
 
MslI CAynn'nnrTG

Cuts at:      0     370    1017 
   Size:        370     647
 
NdeI CA'TA_TG

Cuts at:      0     570    1017 
   Size:        570     447
 
NheI G'CTAG_C

Cuts at:      0     417    1017 
   Size:        417     600
 
NsiI A_TGCA'T

Cuts at:      0     885    1017 
   Size:        885     132
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     144    1017 
   Size:        144     873
 
PfoI T'CCnGG_A

Cuts at:      0     554    1017 
   Size:        554     463
 
PspXI vC'TCGA_Gb

Cuts at:      0     575    1017 
   Size:        575     442
 
SmlI C'TyrA_G

Cuts at:      0     575     812    1017 
   Size:        575     237     205

  Fragments arranged by size: 

                 575     237     205
 
SspI AAT'ATT

Cuts at:      0     186    1017 
   Size:        186     831
 
TatI w'GTAC_w

Cuts at:      0     473    1017 
   Size:        473     544
 
XhoI C'TCGA_G

Cuts at:      0     575    1017 
   Size:        575     442
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI    AlwNI     ApoI     AseI     AvaI
     BaeI     BanI     BbsI    BbvCI    BfrBI     BglI    BglII     BmrI
     BmtI   Bpu10I    BpuEI     BsaI    BseYI    BspMI    BsrDI    BsrGI
    BstYI    BtgZI     BtsI     EaeI     EarI  Eco57MI    EcoNI    EcoRI
    Hin4I  HindIII     KpnI     MscI     MslI     NdeI     NheI     NsiI
    PflMI     PfoI    PspXI     SmlI     SspI     TatI     XhoI

 Enzymes that do not cut: 

    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI    AsiSI    AvrII    BamHI
    BanII     BbeI     BcgI    BciVI     BclI     BlpI Bme1580I    BmgBI
     BplI     BpmI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI
    BspHI    BsrBI    BsrFI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI  BstZ17I   Bsu36I     BtgI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EciI  EcoICRI EcoO109I    EcoRV     FalI     FseI
     FspI    FspAI    HaeII   HincII     HpaI     KasI     MfeI     MluI
     MmeI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NotI     NruI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     StuI     StyI     SwaI    TaqII     TstI  Tth111I
     XbaI     XcmI     XmaI     XmnI     ZraI
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