Restriction map for pF1KB6431
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AflIII      AgeI        AhdI        AlwI        AocI        
Aor51HI     ApoI        AscI        AseI        AsnI        
Asp700I     AspEI       AspI        AviII       BalI        
BamHI       BbrPI       BbsI        BcgI        BciVI       
BclI        BfrI        BglII       BpuAI       BsaAI       
BsaBI       BsgI        BsiWI       BsmBI       BsmI        
Bsp1407I    BspDI       BspHI       BspLU11I    BsrBI       
BsrFI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstXI       BstYI       BstZ17I     
Bsu36I      Cfr10I      ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    Eco47III    Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HindIII     HpaI        
MaeII       MamI        MfeI        MflI        MluI        
MluNI       MscI        MseI        MunI        NaeI        
NcoI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RcaI        RsrII       SacI        SalI        SapI        
SbfI        ScaI        SfiI        SfuI        SgrAI       
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        SwaI        TaqI        Tru9I       
Tsp509I     Tth111I     TthHB8I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        386  GGTACC               G^GTACC
AccIII        116  TCCGGA               T^CCGGA
AcyI          445  GGCGCC               G[GA]^CG[CT]C
ApaI          652  GGGCCC               GGGCC^C
Asp718I       386  GGTACC               G^GTACC
AvrII         811  CCTAGG               C^CTAGG
BbeI          445  GGCGCC               GGCGC^C
BbvCI         744  GCTGAGG              CCTCAGC(-5/-2)
BfaI          812  CTAG                 C^TAG
BglI          616  GCCTCTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI          811  CCTAGG               C^CTAGG
BlpI          235  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      235  GCTGAGC              GC^T[ACGT]AGC
BsaHI         445  GGCGCC               G[GA]^CG[CT]C
BsaWI         116  TCCGGA               [AT]^CCGG[AT]
BseAI         116  TCCGGA               T^CCGGA
BsiEI         920  CGGCCG               CG[GA][CT]^CG
BspEI         116  TCCGGA               T^CCGGA
BspMI         383  GCAGGT               ACCTGC(4/8)
BsrDI         705  CATTGC               GCAATG(2/0)
BssSI         826  CACGAG               CACGAG(-5/-1)
BstAPI        453  GCAGCGACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         235  GCTGAGC              GC^T[ACGT]AGC
DpnII         831  GATC                 ^GATC
EagI          920  CGGCCG               C^GGCCG
EarI          122  GAAGAG               CTCTTC(1/4)
EclXI         920  CGGCCG               C^GGCCG
Eco52I        920  CGGCCG               C^GGCCG
EcoT14I       811  CCTAGG               C^C[AT][AT]GG
HgaI          168  GCGTC                GACGC(5/10)
Hin1I         445  GGCGCC               G[GA]^CG[CT]C
HincII        289  GTCAAC               GT[CT]^[GA]AC
HindII        289  GTCAAC               GT[CT]^[GA]AC
KasI          445  GGCGCC               G^GCGCC
KpnI          386  GGTACC               GGTAC^C
Ksp632I       122  GAAGAG               CTCTTC(1/4)
MaeI          812  CTAG                 C^TAG
MboI          831  GATC                 ^GATC
MboII         122  GAAGA                GAAGA(8/7)
MroI          116  TCCGGA               T^CCGGA
NarI          445  GGCGCC               GG^CGCC
NdeII         831  GATC                 ^GATC
NspI          720  GCATGT               [GA]CATG^[CT]
PpuMI          69  GGGACCC              [GA]G^G[AT]CC[CT]
PvuII         283  CAGCTG               CAG^CTG
Sau3AI        831  GATC                 ^GATC
SfoI          445  GGCGCC               GGC^GCC
StyI          811  CCTAGG               C^C[AT][AT]GG
TfiI          731  GAATC                G^A[AT]TC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AfaI          379  GTAC                 GT^AC
              387  GTAC                
Alw44I        724  GTGCAC               G^TGCAC
              877  GTGCAC              
ApaLI         724  GTGCAC               G^TGCAC
              877  GTGCAC              
AvaI           73  CCCGGG               C^[CT]CG[GA]G
              106  CCCGGG              
BsaI          781  GAGACC               GGTCTC(1/5)
              931  GGTCTC              
BsoBI          73  CCCGGG               C^[CT]CG[GA]G
              106  CCCGGG              
DraII          69  GGGACCC              [GA]G^G[ACGT]CC[CT]
              470  GGGGCCT             
DsaI           85  CCGCGG               C^C[GA][CT]GG
              172  CCGCGG              
EcoO109I       69  GGGACCC              [GA]G^G[ACGT]CC[CT]
              470  GGGGCCT             
KspI           85  CCGCGG               CCGC^GG
              172  CCGCGG              
MaeIII        402  GTCAC                ^GT[ACGT]AC
              436  GTGAC               
RsaI          379  GTAC                 GT^AC
              387  GTAC                
SacII          85  CCGCGG               CCGC^GG
              172  CCGCGG              
SexAI         389  ACCAGGT              A^CC[AT]GGT
              431  ACCAGGT             
SmaI           73  CCCGGG               CCC^GGG
              106  CCCGGG              
SmlI           33  CTTGAG               C^T[CT][GA]AG
              244  CTCAAG              
Tsp45I        402  GTCAC                ^GT[GC]AC
              436  GTGAC               
XmaCI          73  CCCGGG               C^CCGGG
              106  CCCGGG              
XmaI           73  CCCGGG               C^CCGGG
              106  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (   61 enzymes) #
#########################################

AccII       AciI        AluI        AlwNI       AspHI       
AvaII       BanI        BanII       BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        EaeI        
EcoRII      FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NlaIII      NlaIV       PleI        PstI        
Sau96I      ScrFI       SfaNI       SfcI        TseI        
TspRI       
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