Restriction map for pF1KB6373
 (Circular) (Six-base) MAPSORT of: pF1KB6373.seq  Check: 6439  from: 1  to: 3923



pF1KB6373  3923 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:    961     961 
   Size:       3923
 
Acc65I G'GTAC_C

Cuts at:    939     939 
   Size:       3923
 
AclI AA'CG_TT

Cuts at:   3763    3763 
   Size:       3923
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1748    2180    2927    3195    1748 
   Size:        432     747     268    2476

  Fragments arranged by size: 

                2476     747     432     268
 
AflIII A'CryG_T

Cuts at:   1275    2379    3356    1275 
   Size:       1104     977    1842

  Fragments arranged by size: 

                1842    1104     977
 
AgeI A'CCGG_T

Cuts at:   2311    2311 
   Size:       3923
 
AleI CACnn'nnGTG

Cuts at:    892     892 
   Size:       3923
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    850     850 
   Size:       3923
 
AlwNI CAG_nnn'CTG

Cuts at:    438     750    1228    2795     438 
   Size:        312     478    1567    1566

  Fragments arranged by size: 

                1567    1566     478     312
 
ApaI G_GGCC'C

Cuts at:    210     252     210 
   Size:         42    3881
 
ApaLI G'TGCA_C

Cuts at:   2693    2693 
   Size:       3923
 
ApoI r'AATT_y

Cuts at:    927    3276    3532     927 
   Size:       2349     256    1318

  Fragments arranged by size: 

                2349    1318     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3923
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3923
 
AvaI C'yCGr_G

Cuts at:    209     943     209 
   Size:        734    3189
 
AvrII C'CTAG_G

Cuts at:    712     712 
   Size:       3923
 
BamHI G'GATC_C

Cuts at:    948     948 
   Size:       3923
 
BanI G'GyrC_C

Cuts at:    939    1353    1601    1636     939 
   Size:        414     248      35    3226

  Fragments arranged by size: 

                3226     414     248      35
 
BanII G_rGCy'C

Cuts at:    210     252     266     937    1967     210 
   Size:         42      14     671    1030    2166

  Fragments arranged by size: 

                2166    1030     671      42      14
 
BbeI G_GCGC'C

Cuts at:   1357    1605    1357 
   Size:        248    3675
 
BbsI GAAGACnn'nnnn_

Cuts at:    322     322 
   Size:       3923
 
BbvCI CC'TCA_GC

Cuts at:    773     773 
   Size:       3923
 
BciVI GTATCCnnnnn_n'

Cuts at:   1821    2582    3923    1821 
   Size:        761    1341    1821

  Fragments arranged by size: 

                1821    1341     761
 
BclI T'GATC_A

Cuts at:   1443    1443 
   Size:       3923
 
BglI GCCn_nnn'nGGC

Cuts at:    347     761     347 
   Size:        414    3509
 
BglII A'GATC_T

Cuts at:   1438    3475    1438 
   Size:       2037    1886
 
BlpI GC'TnA_GC

Cuts at:   1035    1035 
   Size:       3923
 
Bme1580I G_kGCm'C

Cuts at:    210     252    1548    1641    2697     210 
   Size:         42    1296      93    1056    1436

  Fragments arranged by size: 

                1436    1296    1056      93      42
 
BmrI ACTGGGnnnn_n'

Cuts at:    463     760    1135    1216    1551    3695     463 
   Size:        297     375      81     335    2144     691

  Fragments arranged by size: 

                2144     691     375     335     297      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    429    2290     429 
   Size:       1861    2062
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    274     290     460     510     274 
   Size:         16     170      50    3687

  Fragments arranged by size: 

                3687     170      50      16
 
Bpu10I CC'TnA_GC

Cuts at:    773     773 
   Size:       3923
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1099    1978    2470    2768    1099 
   Size:        879     492     298    2254

  Fragments arranged by size: 

                2254     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    325     403    3627     325 
   Size:         78    3224     621

  Fragments arranged by size: 

                3224     621      78
 
BsaAI yAC'GTr

Cuts at:   1276    1276 
   Size:       3923
 
BsaBI GATnn'nnATC

Cuts at:      6    1437    3474       6 
   Size:       1431    2037     455

  Fragments arranged by size: 

                2037    1431     455
 
BsaHI Gr'CG_yC

Cuts at:    899    1354    1602    2304    3800     899 
   Size:        455     248     702    1496    1022

  Fragments arranged by size: 

                1496    1022     702     455     248
 
BsaWI w'CCGG_w

Cuts at:   1112    1633    2311    2585    2732    3143    1112 
   Size:        521     678     274     147     411    1892

  Fragments arranged by size: 

                1892     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    637     667     637 
   Size:         30    3893
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    421     700     421 
   Size:        279    3644
 
BseYI C'CCAG_C

Cuts at:    175     743     858    1348    2106    2683    3179     175 
   Size:        568     115     490     758     577     496     919

  Fragments arranged by size: 

                 919     758     577     568     496     490     115
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    804     872     804 
   Size:         68    3855
 
BsiEI CG_ry'CG

Cuts at:    104    1465    1511    2719     104 
   Size:       1361      46    1208    1308

  Fragments arranged by size: 

                1361    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    266     889     937    1715    1905    2697     266 
   Size:        623      48     778     190     792    1492

  Fragments arranged by size: 

                1492     792     778     623     190      48
 
Bsp1286I G_dGCh'C

Cuts at:    210     252     266     889     937    1548    1641    1715    1905 
   Size:         42      14     623      48     611      93      74     190

Cuts at:   1905    1967    2697     210 
   Size:         62     730    1436

  Fragments arranged by size: 

                1436     730     623     611     190      93      74      62
                  48      42      14
 
BspEI T'CCGG_A

Cuts at:   3143    3143 
   Size:       3923
 
BspHI T'CATG_A

Cuts at:   3099    3099 
   Size:       3923
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    500     973    1187    1489    1870     500 
   Size:        473     214     302     381    2553

  Fragments arranged by size: 

                2553     473     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2215    2269    3753    3918      50 
   Size:       2165      54    1484     165      55

  Fragments arranged by size: 

                2165    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1835    1835 
   Size:       3923
 
BsrFI r'CCGG_y

Cuts at:   1921    2102    2311    1921 
   Size:        181     209    3533

  Fragments arranged by size: 

                3533     209     181
 
BssHII G'CGCG_C

Cuts at:    186     186 
   Size:       3923
 
BssSI C'ACGA_G

Cuts at:    838    2194    2552     838 
   Size:       1356     358    2209

  Fragments arranged by size: 

                2209    1356     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1300    1300 
   Size:       3923
 
BstBI TT'CG_AA

Cuts at:   2284    2284 
   Size:       3923
 
BstXI CCAn_nnnn'nTGG

Cuts at:    534     609     534 
   Size:         75    3848
 
BstYI r'GATC_y

Cuts at:      1     948    1415    1438    1773    2019    3031    3117    3129 
   Size:        947     467      23     335     246    1012      86      12

Cuts at:   3129    3475       1 
   Size:        346     449

  Fragments arranged by size: 

                1012     947     467     449     346     335     246      86
                  23      12
 
Bsu36I CC'TnA_GG

Cuts at:    404     404 
   Size:       3923
 
BtgI C'CryG_G

Cuts at:    107     893     107 
   Size:        786    3137
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1873    2054    2217    1873 
   Size:        181     163    3579

  Fragments arranged by size: 

                3579     181     163
 
BtsI GCAGTG_nn'

Cuts at:    797    1166     797 
   Size:        369    3554
 
DraI TTT'AAA

Cuts at:    922     922 
   Size:       3923
 
DrdI GACnn_nn'nnGTC

Cuts at:   1629    2487    1629 
   Size:        858    3065
 
EaeI y'GGCC_r

Cuts at:    348     378     546     572    1508    1682    2073    2100    3445 
   Size:         30     168      26     936     174     391      27    1345

Cuts at:   3445     348 
   Size:        826

  Fragments arranged by size: 

                1345     936     826     391     174     168      30      27
                  26
 
EagI C'GGCC_G

Cuts at:   1508    1508 
   Size:       3923
 
EarI CTCTTCn'nnn_

Cuts at:    493    1946    2156     493 
   Size:       1453     210    2260

  Fragments arranged by size: 

                2260    1453     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    328     418    2131    2441    2587    3733     328 
   Size:         90    1713     310     146    1146     518

  Fragments arranged by size: 

                1713    1146     518     310     146      90
 
EcoICRI GAG'CTC

Cuts at:    264     935     264 
   Size:        671    3252
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    274     290     460     510    1748    2180    2927    3195     274 
   Size:         16     170      50    1238     432     747     268    1002

  Fragments arranged by size: 

                1238    1002     747     432     268     170      50      16
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    256     256 
   Size:       3923
 
EcoO109I rG'GnC_Cy

Cuts at:    206     249    1062     206 
   Size:         43     813    3067

  Fragments arranged by size: 

                3067     813      43
 
EcoRI G'AATT_C

Cuts at:    927     927 
   Size:       3923
 
EcoRV GAT'ATC

Cuts at:    909     909 
   Size:       3923
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1942    1942 
   Size:       3923
 
FspI TGC'GCA

Cuts at:   1704    1704 
   Size:       3923
 
HaeII r_GCGC'y

Cuts at:   1357    1605    2627    3606    1357 
   Size:        248    1022     979    1674

  Fragments arranged by size: 

                1674    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    164     196     203     235    1765    1795    1797    1827     164 
   Size:         32       7      32    1530      30       2      30    2260

  Fragments arranged by size: 

                2260    1530      32      32      30      30       7       2
            
 
HincII GTy'rAC

Cuts at:    369     645     962     369 
   Size:        276     317    3330

  Fragments arranged by size: 

                3330     317     276
 
HindIII A'AGCT_T

Cuts at:    605     605 
   Size:       3923
 
KasI G'GCGC_C

Cuts at:   1353    1601    1353 
   Size:        248    3675
 
KpnI G_GTAC'C

Cuts at:    943     943 
   Size:       3923
 
MluI A'CGCG_T

Cuts at:   3356    3356 
   Size:       3923
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    862    2594    2778     862 
   Size:       1732     184    2007

  Fragments arranged by size: 

                2007    1732     184
 
MscI TGG'CCA

Cuts at:    380     574    1684     380 
   Size:        194    1110    2619

  Fragments arranged by size: 

                2619    1110     194
 
MslI CAynn'nnrTG

Cuts at:    892    1912    2039     892 
   Size:       1020     127    2776

  Fragments arranged by size: 

                2776    1020     127
 
MspA1I CmG'CkG

Cuts at:    203     435     678     772    1034    1348    1708    2721    2966 
   Size:        232     243      94     262     314     360    1013     245

Cuts at:   2966     203 
   Size:       1160

  Fragments arranged by size: 

                1160    1013     360     314     262     245     243     232
                  94
 
NaeI GCC'GGC

Cuts at:   2104    2104 
   Size:       3923
 
NarI GG'CG_CC

Cuts at:   1354    1602    1354 
   Size:        248    3675
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3923
 
NdeI CA'TA_TG

Cuts at:    630     630 
   Size:       3923
 
NgoMIV G'CCGG_C

Cuts at:   2102    2102 
   Size:       3923
 
NspI r_CATG'y

Cuts at:    738     976    1476    2383     738 
   Size:        238     500     907    2278

  Fragments arranged by size: 

                2278     907     500     238
 
PasI CC'CwG_GG

Cuts at:    420     420 
   Size:       3923
 
PciI A'CATG_T

Cuts at:   2379    2379 
   Size:       3923
 
PflMI CCAn_nnn'nTGG

Cuts at:    438     750     438 
   Size:        312    3611
 
PmeI GTTT'AAAC

Cuts at:    922     922 
   Size:       3923
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3062    3094    3062 
   Size:         32    3891
 
PspOMI G'GGCC_C

Cuts at:    206     248     206 
   Size:         42    3881
 
PstI C_TGCA'G

Cuts at:    970    1655     970 
   Size:        685    3238
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3923
 
PvuII CAG'CTG

Cuts at:    435    1348    1708     435 
   Size:        913     360    2650

  Fragments arranged by size: 

                2650     913     360
 
RsrII CG'GwC_CG

Cuts at:   2118    2118 
   Size:       3923
 
SacI G_AGCT'C

Cuts at:    266     937     266 
   Size:        671    3252
 
SalI G'TCGA_C

Cuts at:    960     960 
   Size:       3923
 
SapI GCTCTTCn'nnn_

Cuts at:   1946    2156    1946 
   Size:        210    3713
 
SbfI CC_TGCA'GG

Cuts at:    970     970 
   Size:       3923
 
ScaI AGT'ACT

Cuts at:   3440    3440 
   Size:       3923
 
SfcI C'TryA_G

Cuts at:     33     966    1651    2644    2835      33 
   Size:        933     685     993     191    1121

  Fragments arranged by size: 

                1121     993     933     685     191
 
SfiI GGCCn_nnn'nGGCC

Cuts at:    761     761 
   Size:       3923
 
SfoI GGC'GCC

Cuts at:   1355    1603    1355 
   Size:        248    3675
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3923
 
SmaI CCC'GGG

Cuts at:    945     945 
   Size:       3923
 
SmlI C'TyrA_G

Cuts at:   1078    1993    2485    2747    1078 
   Size:        915     492     262    2254

  Fragments arranged by size: 

                2254     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3923
 
SphI G_CATG'C

Cuts at:    738     976    1476     738 
   Size:        238     500    3185

  Fragments arranged by size: 

                3185     500     238
 
StuI AGG'CCT

Cuts at:    402     402 
   Size:       3923
 
StyI C'CwwG_G

Cuts at:    107     578     712    1057    1411     107 
   Size:        471     134     345     354    2619

  Fragments arranged by size: 

                2619     471     354     345     134
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    401    1506    1639    2098    2307     401 
   Size:       1105     133     459     209    2017

  Fragments arranged by size: 

                2017    1105     459     209     133
 
TatI w'GTAC_w

Cuts at:   3438    3438 
   Size:       3923
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3440    3472    3440 
   Size:         32    3891
 
Tth111I GACn'n_nGTC

Cuts at:   1720    1720 
   Size:       3923
 
XbaI T'CTAG_A

Cuts at:    954     954 
   Size:       3923
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    665    1418     665 
   Size:        753    3170
 
XmaI C'CCGG_G

Cuts at:    943     943 
   Size:       3923
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AleI     AlfI
    AlwNI     ApaI    ApaLI     ApoI     AseI    AsiSI     AvaI    AvrII
    BamHI     BanI    BanII     BbeI     BbsI    BbvCI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI   Bsu36I     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FspI    HaeII    Hin4I   HincII  HindIII     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NspI     PasI     PciI    PflMI     PmeI
     PpiI   PspOMI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfiI     SfoI     SgfI     SmaI
     SmlI     SpeI     SphI     StuI     StyI    TaqII     TatI     TstI
  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AloI     AscI     BaeI
     BcgI    BfrBI    BmgBI     BmtI    BsiWI     BsmI    BsmBI    BsrGI
   BstEII  BstZ17I     ClaI    CspCI   DraIII     FseI    FspAI     HpaI
     MfeI     NheI     NotI     NruI     NsiI     PacI     PfoI     PmlI
    PpuMI    PshAI     PsiI    PspXI     PsrI    SacII    SanDI    SexAI
    SgrAI    SnaBI     SrfI     SspI     SwaI     XhoI     XmnI     ZraI
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