Restriction map for pF1KB6368
 (Linear) (Six-base) MAPSORT of: KIBB6368.seq  Check: 9844  from: 1  to: 894



KIBB6368.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     189     894 
   Size:        189     705
 
AccI GT'mk_AC

Cuts at:      0     479     894 
   Size:        479     415
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     894 
   Size:        239     655
 
AleI CACnn'nnGTG

Cuts at:      0     388     894 
   Size:        388     506
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      76     550     894 
   Size:         76     474     344

  Fragments arranged by size: 

                 474     344      76
 
ApoI r'AATT_y

Cuts at:      0     658     894 
   Size:        658     236
 
BanII G_rGCy'C

Cuts at:      0     524     704     894 
   Size:        524     180     190

  Fragments arranged by size: 

                 524     190     180
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     191     283     383     894 
   Size:        191      92     100     511

  Fragments arranged by size: 

                 511     191     100      92
 
Bme1580I G_kGCm'C

Cuts at:      0     566     852     894 
   Size:        566     286      42

  Fragments arranged by size: 

                 566     286      42
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     531     894 
   Size:        531     363
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     287     453     894 
   Size:        287     166     441

  Fragments arranged by size: 

                 441     287     166
 
Bpu10I CC'TnA_GC

Cuts at:      0      97     894 
   Size:         97     797
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     838     894 
   Size:        838      56
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     329     359     894 
   Size:        329      30     535

  Fragments arranged by size: 

                 535     329      30
 
BseYI C'CCAG_C

Cuts at:      0     122     135     333     765     794     894 
   Size:        122      13     198     432      29     100

  Fragments arranged by size: 

                 432     198     122     100      29      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      22     894 
   Size:         22     872
 
BsiEI CG_ry'CG

Cuts at:      0     781     894 
   Size:        781     113
 
BsiHKAI G_wGCw'C

Cuts at:      0     103     524     704     893     894 
   Size:        103     421     180     189       1

  Fragments arranged by size: 

                 421     189     180     103       1
 
Bsp1286I G_dGCh'C

Cuts at:      0     103     524     566     704     852     893     894 
   Size:        103     421      42     138     148      41       1

  Fragments arranged by size: 

                 421     148     138     103      42      41       1
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     189     894 
   Size:        189     705
 
BsrBI CCG'CTC

Cuts at:      0     863     894 
   Size:        863      31
 
BsrFI r'CCGG_y

Cuts at:      0      41     780     894 
   Size:         41     739     114

  Fragments arranged by size: 

                 739     114      41
 
BssHII G'CGCG_C

Cuts at:      0     798     894 
   Size:        798      96
 
BssSI C'ACGA_G

Cuts at:      0     574     894 
   Size:        574     320
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      88     194     390     894 
   Size:         88     106     196     504

  Fragments arranged by size: 

                 504     196     106      88
 
BstYI r'GATC_y

Cuts at:      0     261     894 
   Size:        261     633
 
BstZ17I GTA'TAC

Cuts at:      0     480     894 
   Size:        480     414
 
BtgI C'CryG_G

Cuts at:      0      73     894 
   Size:         73     821
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     471     894 
   Size:        471     423
 
BtsI GCAGTG_nn'

Cuts at:      0      58     468     894 
   Size:         58     410     426

  Fragments arranged by size: 

                 426     410      58
 
DraIII CAC_nnn'GTG

Cuts at:      0     693     894 
   Size:        693     201
 
EaeI y'GGCC_r

Cuts at:      0      90     287     695     752     778     782     884     894 
   Size:         90     197     408      57      26       4     102      10

  Fragments arranged by size: 

                 408     197     102      90      57      26      10       4
            
 
EagI C'GGCC_G

Cuts at:      0     778     894 
   Size:        778     116
 
EarI CTCTTCn'nnn_

Cuts at:      0     617     709     894 
   Size:        617      92     185

  Fragments arranged by size: 

                 617     185      92
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     663     894 
   Size:        663     231
 
EcoICRI GAG'CTC

Cuts at:      0     522     702     894 
   Size:        522     180     192

  Fragments arranged by size: 

                 522     192     180
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     239     287     453     894 
   Size:        239      48     166     441

  Fragments arranged by size: 

                 441     239     166      48
 
EcoO109I rG'GnC_Cy

Cuts at:      0     318     356     894 
   Size:        318      38     538

  Fragments arranged by size: 

                 538     318      38
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     300     609     894 
   Size:        300     309     285

  Fragments arranged by size: 

                 309     300     285
 
FseI GG_CCGG'CC

Cuts at:      0     784     894 
   Size:        784     110
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     181     213     894 
   Size:        181      32     681

  Fragments arranged by size: 

                 681     181      32
 
MscI TGG'CCA

Cuts at:      0     289     894 
   Size:        289     605
 
MslI CAynn'nnrTG

Cuts at:      0     388     574     894 
   Size:        388     186     320

  Fragments arranged by size: 

                 388     320     186
 
MspA1I CmG'CkG

Cuts at:      0     333     534     602     894 
   Size:        333     201      68     292

  Fragments arranged by size: 

                 333     292     201      68
 
NaeI GCC'GGC

Cuts at:      0     782     894 
   Size:        782     112
 
NgoMIV G'CCGG_C

Cuts at:      0     780     894 
   Size:        780     114
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     882     894 
   Size:        882      12
 
PfoI T'CCnGG_A

Cuts at:      0     264     894 
   Size:        264     630
 
PpuMI rG'GwC_Cy

Cuts at:      0     318     356     894 
   Size:        318      38     538

  Fragments arranged by size: 

                 538     318      38
 
PstI C_TGCA'G

Cuts at:      0     455     686     894 
   Size:        455     231     208

  Fragments arranged by size: 

                 455     231     208
 
PvuII CAG'CTG

Cuts at:      0     333     534     894 
   Size:        333     201     360

  Fragments arranged by size: 

                 360     333     201
 
SacI G_AGCT'C

Cuts at:      0     524     704     894 
   Size:        524     180     190

  Fragments arranged by size: 

                 524     190     180
 
SapI GCTCTTCn'nnn_

Cuts at:      0     709     894 
   Size:        709     185
 
SbfI CC_TGCA'GG

Cuts at:      0     455     894 
   Size:        455     439
 
SfcI C'TryA_G

Cuts at:      0     451     682     894 
   Size:        451     231     212

  Fragments arranged by size: 

                 451     231     212
 
SgrAI Cr'CCGG_yG

Cuts at:      0      41     894 
   Size:         41     853
 
SmlI C'TyrA_G

Cuts at:      0     853     894 
   Size:        853      41
 
StyI C'CwwG_G

Cuts at:      0     755     894 
   Size:        755     139
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     181     509     894 
   Size:        181     328     385

  Fragments arranged by size: 

                 385     328     181
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     573     894 
   Size:        573     321
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AleI     AlfI     ApoI    BanII     BbsI
 Bme1580I     BplI     BpmI   Bpu10I    BpuEI    BsaXI    BseYI     BsgI
    BsiEI  BsiHKAI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII    BssSI
    BstXI    BstYI  BstZ17I     BtgI    BtgZI     BtsI   DraIII     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI     FseI
    Hin4I     MscI     MslI   MspA1I     NaeI   NgoMIV    PflMI     PfoI
    PpuMI     PstI    PvuII     SacI     SapI     SbfI     SfcI    SgrAI
     SmlI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AloI    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI    BglII     BlpI    BmgBI     BmrI     BmtI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BsrDI    BsrGI   BstAPI    BstBI   BstEII
   Bsu36I     ClaI    CspCI     DraI     DrdI    EcoNI    EcoRI    EcoRV
     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     NarI     NcoI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI     PmlI
     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
    SacII     SalI    SanDI     ScaI    SexAI     SfiI     SfoI     SgfI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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