Restriction map for pF1KB6359
 (Circular) (Six-base) MAPSORT of: pF1KB6359.seq  Check: 5421  from: 1  to: 3875



pF1KB6359  3875 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:05  ..

AccI GT'mk_AC

Cuts at:    913     913 
   Size:       3875
 
Acc65I G'GTAC_C

Cuts at:    891     891 
   Size:       3875
 
AclI AA'CG_TT

Cuts at:   3715    3715 
   Size:       3875
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    142     262    1700    2132    2879    3147     142 
   Size:        120    1438     432     747     268     870

  Fragments arranged by size: 

                1438     870     747     432     268     120
 
AflIII A'CryG_T

Cuts at:   1227    2331    3308    1227 
   Size:       1104     977    1794

  Fragments arranged by size: 

                1794    1104     977
 
AgeI A'CCGG_T

Cuts at:   2263    2263 
   Size:       3875
 
AlwNI CAG_nnn'CTG

Cuts at:    307     450     534    1180    2747     307 
   Size:        143      84     646    1567    1435

  Fragments arranged by size: 

                1567    1435     646     143      84
 
ApaLI G'TGCA_C

Cuts at:   2645    2645 
   Size:       3875
 
ApoI r'AATT_y

Cuts at:    201     879    3228    3484     201 
   Size:        678    2349     256     592

  Fragments arranged by size: 

                2349     678     592     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       3875
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3875
 
AvaI C'yCGr_G

Cuts at:    895     895 
   Size:       3875
 
BamHI G'GATC_C

Cuts at:    900     900 
   Size:       3875
 
BanI G'GyrC_C

Cuts at:    891    1305    1553    1588     891 
   Size:        414     248      35    3178

  Fragments arranged by size: 

                3178     414     248      35
 
BanII G_rGCy'C

Cuts at:    889    1919     889 
   Size:       1030    2845
 
BbeI G_GCGC'C

Cuts at:   1309    1557    1309 
   Size:        248    3627
 
BbsI GAAGACnn'nnnn_

Cuts at:    194     194 
   Size:       3875
 
BbvCI CC'TCA_GC

Cuts at:    778     778 
   Size:       3875
 
BciVI GTATCCnnnnn_n'

Cuts at:   1773    2534    3875    1773 
   Size:        761    1341    1773

  Fragments arranged by size: 

                1773    1341     761
 
BclI T'GATC_A

Cuts at:   1395    1395 
   Size:       3875
 
BglII A'GATC_T

Cuts at:   1390    3427    1390 
   Size:       2037    1838
 
BlpI GC'TnA_GC

Cuts at:    987     987 
   Size:       3875
 
Bme1580I G_kGCm'C

Cuts at:   1500    1593    2649    1500 
   Size:         93    1056    2726

  Fragments arranged by size: 

                2726    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:    295    1087    1168    1503    3647     295 
   Size:        792      81     335    2144     523

  Fragments arranged by size: 

                2144     792     523     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2242    2242 
   Size:       3875
 
Bpu10I CC'TnA_GC

Cuts at:    778     778 
   Size:       3875
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    130    1051    1930    2422    2720     130 
   Size:        921     879     492     298    1285

  Fragments arranged by size: 

                1285     921     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   3579    3579 
   Size:       3875
 
BsaAI yAC'GTr

Cuts at:   1228    1228 
   Size:       3875
 
BsaBI GATnn'nnATC

Cuts at:      6    1389    3426       6 
   Size:       1383    2037     455

  Fragments arranged by size: 

                2037    1383     455
 
BsaHI Gr'CG_yC

Cuts at:   1306    1554    2256    3752    1306 
   Size:        248     702    1496    1429

  Fragments arranged by size: 

                1496    1429     702     248
 
BsaWI w'CCGG_w

Cuts at:   1064    1585    2263    2537    2684    3095    1064 
   Size:        521     678     274     147     411    1844

  Fragments arranged by size: 

                1844     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:   1300    2058    2635    3131    1300 
   Size:        758     577     496    2044

  Fragments arranged by size: 

                2044     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1417    1463    2671     104 
   Size:       1313      46    1208    1308

  Fragments arranged by size: 

                1313    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    889    1667    1857    2649     889 
   Size:        778     190     792    2115

  Fragments arranged by size: 

                2115     792     778     190
 
Bsp1286I G_dGCh'C

Cuts at:    889    1500    1593    1667    1857    1919    2649     889 
   Size:        611      93      74     190      62     730    2115

  Fragments arranged by size: 

                2115     730     611     190      93      74      62
 
BspEI T'CCGG_A

Cuts at:   3095    3095 
   Size:       3875
 
BspHI T'CATG_A

Cuts at:   3051    3051 
   Size:       3875
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    925    1139    1441    1822     925 
   Size:        214     302     381    2978

  Fragments arranged by size: 

                2978     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50     168    2167    2221    3705    3870      50 
   Size:        118    1999      54    1484     165      55

  Fragments arranged by size: 

                1999    1484     165     118      55      54
 
BsrDI GCAATG_nn'

Cuts at:    858    1787     858 
   Size:        929    2946
 
BsrFI r'CCGG_y

Cuts at:   1873    2054    2263    1873 
   Size:        181     209    3485

  Fragments arranged by size: 

                3485     209     181
 
BssSI C'ACGA_G

Cuts at:   2146    2504    2146 
   Size:        358    3517
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1252    1252 
   Size:       3875
 
BstBI TT'CG_AA

Cuts at:   2236    2236 
   Size:       3875
 
BstYI r'GATC_y

Cuts at:      1     278     482     900    1367    1390    1725    1971    2983 
   Size:        277     204     418     467      23     335     246    1012

Cuts at:   2983    3069    3081    3427       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1012     467     449     418     346     335     277     246
                 204      86      23      12
 
BtgI C'CryG_G

Cuts at:    107     107 
   Size:       3875
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   1825    2006    2169    1825 
   Size:        181     163    3531

  Fragments arranged by size: 

                3531     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1118    1118 
   Size:       3875
 
DraI TTT'AAA

Cuts at:    874     874 
   Size:       3875
 
DrdI GACnn_nn'nnGTC

Cuts at:    347    1581    2439     347 
   Size:       1234     858    1783

  Fragments arranged by size: 

                1783    1234     858
 
EaeI y'GGCC_r

Cuts at:   1460    1634    2025    2052    3397    1460 
   Size:        174     391      27    1345    1938

  Fragments arranged by size: 

                1938    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   1460    1460 
   Size:       3875
 
EarI CTCTTCn'nnn_

Cuts at:   1898    2108    1898 
   Size:        210    3665
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2083    2393    2539    3685    2083 
   Size:        310     146    1146    2273

  Fragments arranged by size: 

                2273    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    887     887 
   Size:       3875
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    142     262    1700    2132    2879    3147     142 
   Size:        120    1438     432     747     268     870

  Fragments arranged by size: 

                1438     870     747     432     268     120
 
EcoO109I rG'GnC_Cy

Cuts at:   1014    1014 
   Size:       3875
 
EcoRI G'AATT_C

Cuts at:    879     879 
   Size:       3875
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1894    1894 
   Size:       3875
 
FspI TGC'GCA

Cuts at:   1656    1656 
   Size:       3875
 
HaeII r_GCGC'y

Cuts at:   1309    1557    2579    3558    1309 
   Size:        248    1022     979    1626

  Fragments arranged by size: 

                1626    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1717    1747    1749    1779    1717 
   Size:         30       2      30    3813

  Fragments arranged by size: 

                3813      30      30       2
 
HincII GTy'rAC

Cuts at:    914     914 
   Size:       3875
 
KasI G'GCGC_C

Cuts at:   1305    1553    1305 
   Size:        248    3627
 
KpnI G_GTAC'C

Cuts at:    895     895 
   Size:       3875
 
MluI A'CGCG_T

Cuts at:   3308    3308 
   Size:       3875
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    353    2546    2730     353 
   Size:       2193     184    1498

  Fragments arranged by size: 

                2193    1498     184
 
MscI TGG'CCA

Cuts at:   1636    1636 
   Size:       3875
 
MslI CAynn'nnrTG

Cuts at:   1864    1991    1864 
   Size:        127    3748
 
MspA1I CmG'CkG

Cuts at:    450     986    1300    1660    2673    2918     450 
   Size:        536     314     360    1013     245    1407

  Fragments arranged by size: 

                1407    1013     536     360     314     245
 
NaeI GCC'GGC

Cuts at:   2056    2056 
   Size:       3875
 
NarI GG'CG_CC

Cuts at:   1306    1554    1306 
   Size:        248    3627
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       3875
 
NgoMIV G'CCGG_C

Cuts at:   2054    2054 
   Size:       3875
 
NspI r_CATG'y

Cuts at:    928    1428    2335     928 
   Size:        500     907    2468

  Fragments arranged by size: 

                2468     907     500
 
PciI A'CATG_T

Cuts at:   2331    2331 
   Size:       3875
 
PflMI CCAn_nnn'nTGG

Cuts at:    450     450 
   Size:       3875
 
PmeI GTTT'AAAC

Cuts at:    874     874 
   Size:       3875
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3014    3046    3014 
   Size:         32    3843
 
PsiI TTA'TAA

Cuts at:    359     359 
   Size:       3875
 
PstI C_TGCA'G

Cuts at:    922    1607     922 
   Size:        685    3190
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       3875
 
PvuII CAG'CTG

Cuts at:    450    1300    1660     450 
   Size:        850     360    2665

  Fragments arranged by size: 

                2665     850     360
 
RsrII CG'GwC_CG

Cuts at:   2070    2070 
   Size:       3875
 
SacI G_AGCT'C

Cuts at:    889     889 
   Size:       3875
 
SalI G'TCGA_C

Cuts at:    912     912 
   Size:       3875
 
SapI GCTCTTCn'nnn_

Cuts at:   1898    2108    1898 
   Size:        210    3665
 
SbfI CC_TGCA'GG

Cuts at:    922     922 
   Size:       3875
 
ScaI AGT'ACT

Cuts at:   3392    3392 
   Size:       3875
 
SfcI C'TryA_G

Cuts at:     33     918    1603    2596    2787      33 
   Size:        885     685     993     191    1121

  Fragments arranged by size: 

                1121     993     885     685     191
 
SfoI GGC'GCC

Cuts at:   1307    1555    1307 
   Size:        248    3627
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       3875
 
SmaI CCC'GGG

Cuts at:    897     897 
   Size:       3875
 
SmlI C'TyrA_G

Cuts at:    145    1030    1945    2437    2699     145 
   Size:        885     915     492     262    1321

  Fragments arranged by size: 

                1321     915     885     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       3875
 
SphI G_CATG'C

Cuts at:    928    1428     928 
   Size:        500    3375
 
StyI C'CwwG_G

Cuts at:    107     396     404    1009    1363     107 
   Size:        289       8     605     354    2619

  Fragments arranged by size: 

                2619     605     354     289       8
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1458    1591    2050    2259    1458 
   Size:        133     459     209    3074

  Fragments arranged by size: 

                3074     459     209     133
 
TatI w'GTAC_w

Cuts at:   3390    3390 
   Size:       3875
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    162     194     392     424    3392    3424     162 
   Size:         32     198      32    2968      32     613

  Fragments arranged by size: 

                2968     613     198      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1672    1672 
   Size:       3875
 
XbaI T'CTAG_A

Cuts at:    646     906     646 
   Size:        260    3615
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1370    1370 
   Size:       3875
 
XmaI C'CCGG_G

Cuts at:    895     895 
   Size:       3875
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII     BbeI
     BbsI    BbvCI    BciVI     BclI    BglII     BlpI Bme1580I     BmrI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseYI    BsiEI  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI
     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI  EcoICRI
  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I   HincII
     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PmeI     PpiI
     PsiI     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI
     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI
     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI
     ApaI     AscI    AvrII     BaeI     BcgI    BfrBI     BglI    BmgBI
     BmtI     BpmI    BsaXI    BseRI     BsgI    BsiWI     BsmI    BsmBI
    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI
   DraIII    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI
     NdeI     NheI     NotI     NruI     NsiI     PacI     PasI     PfoI
     PmlI    PpuMI    PshAI   PspOMI    PspXI     PsrI    SacII    SanDI
    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI
     XhoI     XmnI     ZraI
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