Restriction map for pF1KB6565
##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       AccI        AclI        AcsI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
AocI        ApaLI       ApoI        AscI        AseI        
AsnI        AspEI       AviII       AvrII       BbrPI       
BbvCI       BciVI       BclI        BfaI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaAI       
BsaI        BsgI        BsiWI       Bsp1407I    BspDI       
BspHI       BspLU11I    BsrDI       BsrGI       BssSI       
Bst1107I    BstAPI      BstBI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        Eam1105I    EarI        Eco81I      
EcoNI       EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HincII      HindII      HindIII     
HpaI        Ksp632I     KspI        MaeI        MaeII       
MfeI        MluI        MseI        MslI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        ScaI        SexAI       SfiI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        SspBI       SspI        StuI        SwaI        
Tru9I       Tsp509I     Van91I      XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I        389  GGTACC               G^GTACC
AccIII        809  TCCGGA               T^CCGGA
Aor51HI       815  AGCGCT               AGC^GCT
ApaI          787  GGGCCC               GGGCC^C
Asp700I       480  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I       389  GGTACC               G^GTACC
AspI         1017  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaII          24  GGTCC                G^G[AT]CC
BbeI           73  GGCGCC               GGCGC^C
BcgI          971  GCATCACCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII         729  AGATCT               A^GATCT
BsaBI         522  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI         809  TCCGGA               T^CCGGA
BseRI         196  GAGGAG               GAGGAG(10/8)
BsmAI        1015  GAGAC                GTCTC(1/5)
BsmBI        1015  GAGACG               CGTCTC(1/5)
BsmFI         422  GGGAC                GGGAC(10/14)
BsmI          706  GCATTC               GAATGC(1/-1)
BspEI         809  TCCGGA               T^CCGGA
BsrBI          10  GAGCGG               CCGCTC(-3/-3)
BssHII         55  GCGCGC               G^CGCGC
BstEII        588  GGTCACC              G^GT[ACGT]ACC
BstF5I        831  GGATG                GGATG(2/0)
BstPI         588  GGTCACC              G^GT[ACGT]ACC
DdeI          124  CTGAG                C^T[ACGT]AG
DraII         786  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EagI           43  CGGCCG               C^GGCCG
EclXI          43  CGGCCG               C^GGCCG
Eco47III      815  AGCGCT               AGC^GCT
Eco52I         43  CGGCCG               C^GGCCG
EcoO109I      786  GGGGCCC              [GA]G^G[ACGT]CC[CT]
EcoO65I       588  GGTCACC              G^GT[ACGT]ACC
FauI          936  CCCGC                CCCGC(4/6)
FokI          831  GGATG                GGATG(9/13)
KasI           73  GGCGCC               G^GCGCC
KpnI          389  GGTACC               GGTAC^C
MamI          522  GATGAAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI          809  TCCGGA               T^CCGGA
MspA1I         40  CAGCGG               C[AC]G^C[GT]G
NaeI          555  GCCGGC               GCC^GGC
NarI           73  GGCGCC               GG^CGCC
NcoI          827  CCATGG               C^CATGG
NgoMIV        555  GCCGGC               G^CCGGC
PflFI        1017  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SfoI           73  GGCGCC               GGC^GCC
TfiI          137  GAATC                G^A[AT]TC
Tth111I      1017  GACGGAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          957  CCACTGGCCACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          480  GAAGTATTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AspHI         473  GTGCTC               G[AT]GC[AT]^C
              532  GAGCAC              
AvaI          132  CTCGGG               C^[CT]CG[GA]G
              878  CCCGAG              
BalI            2  TGGCCA               TGG^CCA
              961  TGGCCA              
BamHI          81  GGATCC               G^GATCC
              323  GGATCC              
BpmI          747  CTCCAG               CTGGAG(16/14)
               28  CTGGAG              
BsaWI         143  ACCGGA               [AT]^CCGG[AT]
              809  TCCGGA              
BsiEI          43  CGGCCG               CG[GA][CT]^CG
              990  CGACCG              
BsiHKAI       473  GTGCTC               G[AT]GC[AT]^C
              532  GAGCAC              
BsoBI         132  CTCGGG               C^[CT]CG[GA]G
              878  CCCGAG              
BsrFI         281  ACCGGC               [GA]^CCGG[CT]
              555  GCCGGC              
Cfr10I        281  ACCGGC               [GA]^CCGG[CT]
              555  GCCGGC              
HaeII          73  GGCGCC               [GA]GCGC^[CT]
              815  AGCGCT              
MluNI           2  TGGCCA               TGG^CCA
              961  TGGCCA              
MscI            2  TGGCCA               TGG^CCA
              961  TGGCCA              
PleI          256  GAGTC                GAGTC(4/5)
             1021  GAGTC               
SbfI          582  CCTGCAGG             CCTGCA^GG
              696  CCTGCAGG            
SfaNI         499  GATGC                GCATC(5/9)
              971  GCATC               
Sse8387I      582  CCTGCAGG             CCTGCA^GG
              696  CCTGCAGG            
TaqI          312  TCGA                 T^CGA
              530  TCGA                
Tsp45I        589  GTCAC                ^GT[GC]AC
              982  GTCAC               
TthHB8I       312  TCGA                 T^CGA
              530  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   66 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        BanI        BanII       BbsI        BbvI        
BcnI        BmyI        BpuAI       BsaHI       BsaJI       
BsiYI       BslI        Bsp1286I    BspMI       BsrI        
BssKI       BstNI       BstUI       BstYI       Cac8I       
CfoI        Cfr13I      DpnII       DsaI        EaeI        
EcoRII      EcoT14I     Fnu4HI      HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeIII      MboI        
MboII       MflI        MnlI        MspI        MvaI        
MvnI        MwoI        NciI        NdeII       NlaIII      
NlaIV       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        StyI        TseI        TspRI       
XhoII       
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