Restriction map for pF1KB6565
 (Linear) (Six-base) MAPSORT of: KIBB6565.seq  Check: 5898  from: 1  to: 1122



KIBB6565.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:26  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     690    1122 
   Size:        690     432
 
Acc65I G'GTAC_C

Cuts at:      0     389    1122 
   Size:        389     733
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     499     619     781    1122 
   Size:        499     120     162     341

  Fragments arranged by size: 

                 499     341     162     120
 
AfeI AGC'GCT

Cuts at:      0     817    1122 
   Size:        817     305
 
ApaI G_GGCC'C

Cuts at:      0     791    1122 
   Size:        791     331
 
AvaI C'yCGr_G

Cuts at:      0     132     878    1122 
   Size:        132     746     244

  Fragments arranged by size: 

                 746     244     132
 
BamHI G'GATC_C

Cuts at:      0      81     323    1122 
   Size:         81     242     799

  Fragments arranged by size: 

                 799     242      81
 
BanI G'GyrC_C

Cuts at:      0      73     389     735    1122 
   Size:         73     316     346     387

  Fragments arranged by size: 

                 387     346     316      73
 
BanII G_rGCy'C

Cuts at:      0     377     512     791    1122 
   Size:        377     135     279     331

  Fragments arranged by size: 

                 377     331     279     135
 
BbeI G_GCGC'C

Cuts at:      0      77    1122 
   Size:         77    1045
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     105     787     882    1122 
   Size:        105     682      95     240

  Fragments arranged by size: 

                 682     240     105      95
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     960     994    1122 
   Size:        960      34     128

  Fragments arranged by size: 

                 960     128      34
 
BglII A'GATC_T

Cuts at:      0     729    1122 
   Size:        729     393
 
Bme1580I G_kGCm'C

Cuts at:      0     791    1122 
   Size:        791     331
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      99    1122 
   Size:         99    1023
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     396    1122 
   Size:        396     726
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     732    1122 
   Size:         49     683     390

  Fragments arranged by size: 

                 683     390      49
 
Bpu10I CC'TnA_GC

Cuts at:      0     124    1122 
   Size:        124     998
 
BsaBI GATnn'nnATC

Cuts at:      0     526    1122 
   Size:        526     596
 
BsaHI Gr'CG_yC

Cuts at:      0      74     110     188     236     869    1122 
   Size:         74      36      78      48     633     253

  Fragments arranged by size: 

                 633     253      78      74      48      36
 
BsaWI w'CCGG_w

Cuts at:      0     143     809    1122 
   Size:        143     666     313

  Fragments arranged by size: 

                 666     313     143
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      79     109    1122 
   Size:         79      30    1013

  Fragments arranged by size: 

                1013      79      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     211    1122 
   Size:        211     911
 
BsiEI CG_ry'CG

Cuts at:      0      46     993    1122 
   Size:         46     947     129

  Fragments arranged by size: 

                 947     129      46
 
BsiHKAI G_wGCw'C

Cuts at:      0     477     536    1122 
   Size:        477      59     586

  Fragments arranged by size: 

                 586     477      59
 
BsmI GAATG_Cn'

Cuts at:      0     706    1122 
   Size:        706     416
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1009    1122 
   Size:       1009     113
 
Bsp1286I G_dGCh'C

Cuts at:      0     377     477     512     536     791    1122 
   Size:        377     100      35      24     255     331

  Fragments arranged by size: 

                 377     331     255     100      35      24
 
BspEI T'CCGG_A

Cuts at:      0     809    1122 
   Size:        809     313
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     576     690     704    1122 
   Size:        576     114      14     418

  Fragments arranged by size: 

                 576     418     114      14
 
BsrBI CCG'CTC

Cuts at:      0      12    1122 
   Size:         12    1110
 
BsrFI r'CCGG_y

Cuts at:      0     281     555    1122 
   Size:        281     274     567

  Fragments arranged by size: 

                 567     281     274
 
BssHII G'CGCG_C

Cuts at:      0      55    1122 
   Size:         55    1067
 
BstEII G'GTnAC_C

Cuts at:      0     588    1122 
   Size:        588     534
 
BstYI r'GATC_y

Cuts at:      0      81     323     729    1122 
   Size:         81     242     406     393

  Fragments arranged by size: 

                 406     393     242      81
 
BtgI C'CryG_G

Cuts at:      0     225     576     827    1122 
   Size:        225     351     251     295

  Fragments arranged by size: 

                 351     295     251     225
 
BtsI GCAGTG_nn'

Cuts at:      0     325    1100    1122 
   Size:        325     775      22

  Fragments arranged by size: 

                 775     325      22
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     891     926    1122 
   Size:        891      35     196

  Fragments arranged by size: 

                 891     196      35
 
EaeI y'GGCC_r

Cuts at:      0       2      43     417     557     567     961    1122 
   Size:          2      41     374     140      10     394     161

  Fragments arranged by size: 

                 394     374     161     140      41      10       2
 
EagI C'GGCC_G

Cuts at:      0      43    1122 
   Size:         43    1079
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     169    1122 
   Size:        169     953
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      49     499     619     732     781    1122 
   Size:         49     450     120     113      49     341

  Fragments arranged by size: 

                 450     341     120     113      49      49
 
EcoO109I rG'GnC_Cy

Cuts at:      0     787    1122 
   Size:        787     335
 
HaeII r_GCGC'y

Cuts at:      0      77     819    1122 
   Size:         77     742     303

  Fragments arranged by size: 

                 742     303      77
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      79     111     872     904    1006    1038    1122 
   Size:         79      32     761      32     102      32      84

  Fragments arranged by size: 

                 761     102      84      79      32      32      32
 
KasI G'GCGC_C

Cuts at:      0      73    1122 
   Size:         73    1049
 
KpnI G_GTAC'C

Cuts at:      0     393    1122 
   Size:        393     729
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     320    1049    1122 
   Size:        320     729      73

  Fragments arranged by size: 

                 729     320      73
 
MscI TGG'CCA

Cuts at:      0       4     963    1122 
   Size:          4     959     159

  Fragments arranged by size: 

                 959     159       4
 
MspA1I CmG'CkG

Cuts at:      0      42    1122 
   Size:         42    1080
 
NaeI GCC'GGC

Cuts at:      0     557    1122 
   Size:        557     565
 
NarI GG'CG_CC

Cuts at:      0      74    1122 
   Size:         74    1048
 
NcoI C'CATG_G

Cuts at:      0     827    1122 
   Size:        827     295
 
NgoMIV G'CCGG_C

Cuts at:      0     555    1122 
   Size:        555     567
 
PasI CC'CwG_GG

Cuts at:      0     712     948    1122 
   Size:        712     236     174

  Fragments arranged by size: 

                 712     236     174
 
PfoI T'CCnGG_A

Cuts at:      0      26     748    1122 
   Size:         26     722     374

  Fragments arranged by size: 

                 722     374      26
 
PspOMI G'GGCC_C

Cuts at:      0     787    1122 
   Size:        787     335
 
PstI C_TGCA'G

Cuts at:      0      41     587     701    1122 
   Size:         41     546     114     421

  Fragments arranged by size: 

                 546     421     114      41
 
SbfI CC_TGCA'GG

Cuts at:      0     587     701    1122 
   Size:        587     114     421

  Fragments arranged by size: 

                 587     421     114
 
SfcI C'TryA_G

Cuts at:      0      37     583     697    1122 
   Size:         37     546     114     425

  Fragments arranged by size: 

                 546     425     114      37
 
SfoI GGC'GCC

Cuts at:      0      75    1122 
   Size:         75    1047
 
StyI C'CwwG_G

Cuts at:      0       5     503     512     827    1122 
   Size:          5     498       9     315     295

  Fragments arranged by size: 

                 498     315     295       9       5
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     961    1029    1122 
   Size:        961      68      93

  Fragments arranged by size: 

                 961      93      68
 
TatI w'GTAC_w

Cuts at:      0     848    1122 
   Size:        848     274
 
Tth111I GACn'n_nGTC

Cuts at:      0    1020    1122 
   Size:       1020     102
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     964    1122 
   Size:        964     158
 
XmnI GAAnn'nnTTC

Cuts at:      0     484    1122 
   Size:        484     638
 

 Enzymes that do cut:

     AarI   Acc65I     AcuI     AfeI     ApaI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI     BcgI    BglII Bme1580I     BmrI     BplI
     BpmI   Bpu10I    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BsiEI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI    BsrFI
   BssHII   BstEII    BstYI     BtgI     BtsI    CspCI     EaeI     EagI
     EciI  Eco57MI EcoO109I    HaeII    Hin4I     KasI     KpnI     MmeI
     MscI   MspA1I     NaeI     NarI     NcoI   NgoMIV     PasI     PfoI
   PspOMI     PstI     SbfI     SfcI     SfoI     StyI    TaqII     TatI
  Tth111I     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI     AclI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI    AlwNI    ApaLI     ApoI     AscI     AseI    AsiSI
    AvrII     BaeI    BbvCI    BciVI     BclI    BfrBI     BglI     BlpI
    BmgBI     BmtI    BpuEI     BsaI    BsaAI    BseYI     BsgI    BsiWI
    BspHI    BsrDI    BsrGI    BssSI   BstAPI    BstBI    BstXI  BstZ17I
   Bsu36I    BtgZI     ClaI     DraI   DraIII     DrdI     EarI  EcoICRI
    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI   HincII
  HindIII     HpaI     MfeI     MluI     MslI     NdeI     NheI     NotI
     NruI     NsiI     NspI     PacI     PciI    PflMI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI    PspXI     PsrI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     ScaI    SexAI
     SfiI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TstI     XbaI     XhoI     XmaI
     ZraI
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