Restriction map for pF1KB6342
##################################
# NO CUT ENZYMES (  129 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AocI        Aor51HI     
ApaI        ApaLI       ApoI        AscI        AseI        
AsnI        Asp718I     AspEI       AviII       AvrII       
BalI        BamHI       BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlnI        BsaBI       
BsaI        BsaWI       BseAI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrGI       BsrI        BssSI       Bst1107I    BstBI       
BstXI       BstYI       BstZ17I     Bsu36I      ClaI        
CpoI        DpnII       DraI        DraIII      Eam1105I    
Eco47III    Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FspI        HincII      HindII      HindIII     
HpaI        KpnI        KspI        MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MseI        MunI        NdeI        NdeII       
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsaI        RsrII       SacI        SacII       SalI        
Sau3AI      ScaI        SfiI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
SspBI       SspI        SwaI        TfiI        Tru9I       
Tsp509I     TspRI       Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AlwNI         634  CAGCCCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AspI          309  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          111  CCCGAG               C^[CT]CG[GA]G
BanI          777  GGCGCC               G^G[CT][GA]CC
BanII         841  GAGCCC               G[GA]GC[CT]^C
BbeI          777  GGCGCC               GGCGC^C
BbrPI         748  CACGTG               CAC^GTG
BbsI          817  GAAGAC               GAAGAC(2/6)
BfaI          560  CTAG                 C^TAG
BlpI          426  GCTGAGC              GC^T[ACGT]AGC
BpmI          715  CTGGAG               CTGGAG(16/14)
Bpu1102I      426  GCTGAGC              GC^T[ACGT]AGC
BpuAI         817  GAAGAC               GAAGAC(2/6)
BsaAI         748  CACGTG               [CT]AC^GT[GA]
BsmAI         523  GAGAC                GTCTC(1/5)
BsmBI         523  GAGACG               CGTCTC(1/5)
BsoBI         111  CCCGAG               C^[CT]CG[GA]G
BspMI         699  GCAGGT               ACCTGC(4/8)
BsrDI         450  CATTGC               GCAATG(2/0)
BssHII        148  GCGCGC               G^CGCGC
BstAPI        696  GCAGCAGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII         426  GCTGAGC              GC^T[ACGT]AGC
DrdI          525  GACGCTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI         480  CCTCCCCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI           81  GGCCGGCC             GGCCGG^CC
HinfI         250  GACTC                G^A[ACGT]TC
KasI          777  GGCGCC               G^GCGCC
MaeI          560  CTAG                 C^TAG
MslI           88  CACAGGGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI           82  GCCGGC               GCC^GGC
NarI          777  GGCGCC               GG^CGCC
NgoMIV         82  GCCGGC               G^CCGGC
NspI          737  ACATGC               [GA]CATG^[CT]
PflFI         309  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PleI          250  GACTC                GAGTC(4/5)
PmaCI         748  CACGTG               CAC^GTG
PmlI          748  CACGTG               CAC^GTG
PpuMI         504  GGGACCT              [GA]G^G[AT]CC[CT]
PstI          493  CTGCAG               CTGCA^G
PvuII         142  CAGCTG               CAG^CTG
SbfI          492  CCTGCAGG             CCTGCA^GG
SexAI         260  ACCAGGT              A^CC[AT]GGT
SfaNI         204  GATGC                GCATC(5/9)
SfoI          777  GGCGCC               GGC^GCC
Sse8387I      492  CCTGCAGG             CCTGCA^GG
StuI          791  AGGCCT               AGG^CCT
Tth111I       309  GACGGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcyI          312  GGCGTC               G[GA]^CG[CT]C
              777  GGCGCC              
Asp700I       120  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              438  GAACGCCTTC          
AspHI         177  GAGCAC               G[AT]GC[AT]^C
              865  GAGCAC              
BbvCI         454  GCTGAGG              CCTCAGC(-5/-2)
              799  GCTGAGG             
BsaHI         312  GGCGTC               G[GA]^CG[CT]C
              777  GGCGCC              
BsiEI          80  CGGCCG               CG[GA][CT]^CG
              590  CGGCCG              
BsiHKAI       177  GAGCAC               G[AT]GC[AT]^C
              865  GAGCAC              
BsmFI         504  GGGAC                GGGAC(10/14)
              579  GGGAC               
BsrFI          82  GCCGGC               [GA]^CCGG[CT]
              330  GCCGGT              
BstEII        333  GGTGACC              G^GT[ACGT]ACC
              657  GGTGACC             
BstF5I         93  GGATG                GGATG(2/0)
              519  GGATG               
BstPI         333  GGTGACC              G^GT[ACGT]ACC
              657  GGTGACC             
Cfr10I         82  GCCGGC               [GA]^CCGG[CT]
              330  GCCGGT              
DraII         488  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              504  GGGACCT             
EagI           80  CGGCCG               C^GGCCG
              590  CGGCCG              
EclXI          80  CGGCCG               C^GGCCG
              590  CGGCCG              
Eco52I         80  CGGCCG               C^GGCCG
              590  CGGCCG              
EcoO109I      488  AGGGCCT              [GA]G^G[ACGT]CC[CT]
              504  GGGACCT             
EcoO65I       333  GGTGACC              G^GT[ACGT]ACC
              657  GGTGACC             
FokI           93  GGATG                GGATG(9/13)
              519  GGATG               
HgaI          525  GACGC                GACGC(5/10)
              313  GCGTC               
Hin1I         312  GGCGTC               G[GA]^CG[CT]C
              777  GGCGCC              
HphI          333  GGTGA                GGTGA(8/7)
              657  GGTGA               
MaeII         749  ACGT                 A^CGT
              869  ACGT                
MspA1I        142  CAGCTG               C[AC]G^C[GT]G
              781  CCGCTG              
NcoI          167  CCATGG               C^CATGG
              473  CCATGG              
SapI          685  GAAGAGC              GCTCTTC(1/4)
              408  GCTCTTC             
SfcI          105  CTATAG               C^T[GA][CT]AG
              493  CTGCAG              
TaqI          185  TCGA                 T^CGA
              446  TCGA                
TthHB8I       185  TCGA                 T^CGA
              446  TCGA                
XmnI          120  GAAGAAGTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              438  GAACGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   51 enzymes) #
#########################################

AccII       AciI        AluI        AvaII       BbvI        
BcnI        BmyI        BsaJI       BseRI       BsgI        
BsiYI       BslI        Bsp1286I    BsrBI       BssKI       
BstNI       BstUI       Cac8I       CfoI        Cfr13I      
DdeI        DsaI        EaeI        EarI        EcoRII      
EcoT14I     FauI        Fnu4HI      HaeII       HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
Ksp632I     MaeIII      MboII       MnlI        MspI        
MvaI        MvnI        MwoI        NciI        NlaIII      
NlaIV       Sau96I      ScrFI       StyI        TseI        
Tsp45I      
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