Restriction map for
pF1KB6342
(Linear) (Six-base) MAPSORT of: KIBB6342.seq Check: 9954 from: 1 to: 873
KIBB6342.orf
With 182 enzymes: SgfI *
April 24, 2009 13:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 690 873
Size: 690 183
AlwNI CAG_nnn'CTG
Cuts at: 0 639 873
Size: 639 234
AvaI C'yCGr_G
Cuts at: 0 111 873
Size: 111 762
BanI G'GyrC_C
Cuts at: 0 777 873
Size: 777 96
BanII G_rGCy'C
Cuts at: 0 845 873
Size: 845 28
BbeI G_GCGC'C
Cuts at: 0 781 873
Size: 781 92
BbsI GAAGACnn'nnnn_
Cuts at: 0 824 873
Size: 824 49
BbvCI CC'TCA_GC
Cuts at: 0 455 800 873
Size: 455 345 73
Fragments arranged by size:
455 345 73
BlpI GC'TnA_GC
Cuts at: 0 427 873
Size: 427 446
Bme1580I G_kGCm'C
Cuts at: 0 25 62 873
Size: 25 37 811
Fragments arranged by size:
811 37 25
BmgBI CAC'GTC
Cuts at: 0 870 873
Size: 870 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 873
Size: 736 137
Bpu10I CC'TnA_GC
Cuts at: 0 175 455 800 873
Size: 175 280 345 73
Fragments arranged by size:
345 280 175 73
BsaAI yAC'GTr
Cuts at: 0 750 873
Size: 750 123
BsaHI Gr'CG_yC
Cuts at: 0 313 778 873
Size: 313 465 95
Fragments arranged by size:
465 313 95
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 238 268 873
Size: 238 30 605
Fragments arranged by size:
605 238 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 243 472 733 769 838 841 844 847
Size: 243 229 261 36 69 3 3 3
Cuts at: 847 850 853 873
Size: 3 3 20
Fragments arranged by size:
261 243 229 69 36 20 3 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 41 355 873
Size: 41 314 518
Fragments arranged by size:
518 314 41
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 498 538 715 873
Size: 498 40 177 158
Fragments arranged by size:
498 177 158 40
BsiEI CG_ry'CG
Cuts at: 0 83 593 873
Size: 83 510 280
Fragments arranged by size:
510 280 83
BsiHKAI G_wGCw'C
Cuts at: 0 181 869 873
Size: 181 688 4
Fragments arranged by size:
688 181 4
BsmBI CGTCTCn'nnnn_
Cuts at: 0 517 873
Size: 517 356
Bsp1286I G_dGCh'C
Cuts at: 0 25 62 181 845 869 873
Size: 25 37 119 664 24 4
Fragments arranged by size:
664 119 37 25 24 4
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 690 873
Size: 690 183
BsrBI CCG'CTC
Cuts at: 0 6 116 431 873
Size: 6 110 315 442
Fragments arranged by size:
442 315 110 6
BsrDI GCAATG_nn'
Cuts at: 0 449 873
Size: 449 424
BsrFI r'CCGG_y
Cuts at: 0 82 330 873
Size: 82 248 543
Fragments arranged by size:
543 248 82
BssHII G'CGCG_C
Cuts at: 0 148 873
Size: 148 725
BstAPI GCAn_nnn'nTGC
Cuts at: 0 702 873
Size: 702 171
BstEII G'GTnAC_C
Cuts at: 0 333 657 873
Size: 333 324 216
Fragments arranged by size:
333 324 216
BtgI C'CryG_G
Cuts at: 0 167 473 596 873
Size: 167 306 123 277
Fragments arranged by size:
306 277 167 123
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 525 873
Size: 525 348
DrdI GACnn_nn'nnGTC
Cuts at: 0 531 873
Size: 531 342
EaeI y'GGCC_r
Cuts at: 0 80 84 536 590 731 873
Size: 80 4 452 54 141 142
Fragments arranged by size:
452 142 141 80 54 4
EagI C'GGCC_G
Cuts at: 0 80 590 873
Size: 80 510 283
Fragments arranged by size:
510 283 80
EarI CTCTTCn'nnn_
Cuts at: 0 4 415 680 873
Size: 4 411 265 193
Fragments arranged by size:
411 265 193 4
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 736 873
Size: 736 137
EcoNI CCTnn'n_nnAGG
Cuts at: 0 484 873
Size: 484 389
EcoO109I rG'GnC_Cy
Cuts at: 0 489 505 873
Size: 489 16 368
Fragments arranged by size:
489 368 16
FseI GG_CCGG'CC
Cuts at: 0 86 873
Size: 86 787
HaeII r_GCGC'y
Cuts at: 0 31 295 551 727 781 873
Size: 31 264 256 176 54 92
Fragments arranged by size:
264 256 176 92 54 31
KasI G'GCGC_C
Cuts at: 0 777 873
Size: 777 96
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 873
Size: 281 592
MslI CAynn'nnrTG
Cuts at: 0 92 873
Size: 92 781
MspA1I CmG'CkG
Cuts at: 0 144 783 873
Size: 144 639 90
Fragments arranged by size:
639 144 90
NaeI GCC'GGC
Cuts at: 0 84 873
Size: 84 789
NarI GG'CG_CC
Cuts at: 0 778 873
Size: 778 95
NcoI C'CATG_G
Cuts at: 0 167 473 873
Size: 167 306 400
Fragments arranged by size:
400 306 167
NgoMIV G'CCGG_C
Cuts at: 0 82 873
Size: 82 791
NspI r_CATG'y
Cuts at: 0 741 873
Size: 741 132
PmlI CAC'GTG
Cuts at: 0 750 873
Size: 750 123
PpuMI rG'GwC_Cy
Cuts at: 0 505 873
Size: 505 368
PstI C_TGCA'G
Cuts at: 0 497 873
Size: 497 376
PvuII CAG'CTG
Cuts at: 0 144 873
Size: 144 729
SapI GCTCTTCn'nnn_
Cuts at: 0 415 680 873
Size: 415 265 193
Fragments arranged by size:
415 265 193
SbfI CC_TGCA'GG
Cuts at: 0 497 873
Size: 497 376
SexAI A'CCwGG_T
Cuts at: 0 260 873
Size: 260 613
SfcI C'TryA_G
Cuts at: 0 105 493 873
Size: 105 388 380
Fragments arranged by size:
388 380 105
SfoI GGC'GCC
Cuts at: 0 779 873
Size: 779 94
StuI AGG'CCT
Cuts at: 0 793 873
Size: 793 80
StyI C'CwwG_G
Cuts at: 0 167 473 485 873
Size: 167 306 12 388
Fragments arranged by size:
388 306 167 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 676 873
Size: 676 197
Tth111I GACn'n_nGTC
Cuts at: 0 312 873
Size: 312 561
XmnI GAAnn'nnTTC
Cuts at: 0 124 442 873
Size: 124 318 431
Fragments arranged by size:
431 318 124
Enzymes that do cut:
AarI AlwNI AvaI BanI BanII BbeI BbsI BbvCI
BlpI Bme1580I BmgBI BpmI Bpu10I BsaAI BsaHI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssHII BstAPI BstEII BtgI BtgZI
DrdI EaeI EagI EarI Eco57MI EcoNI EcoO109I FseI
HaeII KasI MmeI MslI MspA1I NaeI NarI NcoI
NgoMIV NspI PmlI PpuMI PstI PvuII SapI SbfI
SexAI SfcI SfoI StuI StyI TaqII Tth111I XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BcgI BciVI
BclI BfrBI BglI BglII BmrI BmtI BplI BpuEI
BsaI BsaBI BsaWI BsiWI BsmI BspEI BspHI BsrGI
BssSI BstBI BstXI BstYI BstZ17I Bsu36I BtsI ClaI
CspCI DraI DraIII EciI EcoICRI EcoRI EcoRV FalI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MscI NdeI NheI NotI NruI NsiI PacI
PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI ScaI SfiI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI SspI SwaI TatI TstI XbaI
XcmI XhoI XmaI ZraI