Restriction map for pF1KE0990
##################################
# NO CUT ENZYMES (  116 enzymes) #
##################################

Acc65I      AclI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AvaI        AviII       AvrII       BamHI       
BbeI        BbrPI       BcgI        BciVI       BfaI        
BfrI        BglI        BlnI        BsaBI       BsaI        
BsiEI       BsiWI       BsmI        BsoBI       Bsp1407I    
BspDI       BspHI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstZ17I     Bsu36I      ClaI        CpoI        DraI        
DraIII      EagI        Eam1105I    EclXI       Eco47III    
Eco52I      Eco81I      EcoO65I     EcoRI       EcoRV       
EcoT22I     FseI        FspI        HaeII       HindIII     
HpaI        KasI        KpnI        KspI        MaeI        
MamI        MfeI        MluI        MseI        MunI        
NarI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PflMI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsrII       
SacII       SalI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SspBI       SwaI        Tru9I       Tsp45I      
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   39 enzymes) #
###################################

AccI          670  GTCTAC               GT^[AC][GT]AC
AcsI         1292  AAATTC               [GA]^AATT[CT]
AlwI          742  GGATC                GGATC(4/5)
ApoI         1292  AAATTC               [GA]^AATT[CT]
Asp700I       693  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          604  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BanI         1635  GGCACC               G^G[CT][GA]CC
BbvCI         367  GCTGAGG              CCTCAGC(-5/-2)
BclI          104  TGATCA               T^GATCA
BglII        1358  AGATCT               A^GATCT
BlpI           66  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I       66  GCTCAGC              GC^T[ACGT]AGC
BsaAI         100  TACGTG               [CT]AC^GT[GA]
BsgI          151  GTGCAG               GTGCAG(16/14)
BsmBI        1519  GAGACG               CGTCTC(1/5)
BsrDI        1240  GCAATG               GCAATG(2/0)
BstAPI       1645  GCAGGGGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1625  CCATGAGTGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI        1358  AGATCT               [GA]^GATC[CT]
CelII          66  GCTCAGC              GC^T[ACGT]AGC
DrdI          890  GACTGCAGGGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI          104  TGATCA               T^GATCA
HgaI           61  GACGC                GACGC(5/10)
MflI         1358  AGATCT               [GA]^GATC[CT]
NaeI          450  GCCGGC               GCC^GGC
NcoI          140  CCATGG               C^CATGG
NgoMIV        450  GCCGGC               G^CCGGC
NspI          194  GCATGC               [GA]CATG^[CT]
PflFI         604  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SbfI         1464  CCTGCAGG             CCTGCA^GG
SmlI          492  CTTGAG               C^T[CT][GA]AG
SphI          194  GCATGC               GCATG^C
Sse8387I     1464  CCTGCAGG             CCTGCA^GG
SspI         1822  AATATT               AAT^ATT
StuI         1733  AGGCCT               AGG^CCT
TspRI        1287  CAGTG                CA[GC]TG[ACGT][ACGT]^
Tth111I       604  GACGTGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoII        1358  AGATCT               [GA]^GATC[CT]
XmnI          693  GAAGCCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AatII         667  GACGTC               GACGT^C
             1128  GACGTC              
AccIII       1124  TCCGGA               T^CCGGA
             1398  TCCGGA              
AcyI          667  GACGTC               G[GA]^CG[CT]C
             1128  GACGTC              
BalI          404  TGGCCA               TGG^CCA
             1421  TGGCCA              
BsaHI         667  GACGTC               G[GA]^CG[CT]C
             1128  GACGTC              
BsaWI        1124  TCCGGA               [AT]^CCGG[AT]
             1398  TCCGGA              
BseAI        1124  TCCGGA               T^CCGGA
             1398  TCCGGA              
BspEI        1124  TCCGGA               T^CCGGA
             1398  TCCGGA              
BspMI         974  ACCTGC               ACCTGC(4/8)
             1118  ACCTGC              
BsrBI         145  GAGCGG               CCGCTC(-3/-3)
             1501  GAGCGG              
BsrFI         282  ACCGGC               [GA]^CCGG[CT]
              450  GCCGGC              
BsrI           80  ACTGG                ACTGG(1/-1)
              275  ACTGG               
Cfr10I        282  ACCGGC               [GA]^CCGG[CT]
              450  GCCGGC              
DsaI          140  CCATGG               C^C[GA][CT]GG
             1424  CCACGG              
EcoNI        1374  CCTGGAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
             1464  CCTGCAGGAGG         
EcoT14I       140  CCATGG               C^C[AT][AT]GG
             1531  CCTTGG              
Hin1I         667  GACGTC               G[GA]^CG[CT]C
             1128  GACGTC              
HincII       1081  GTCAAC               GT[CT]^[GA]AC
             1594  GTTGAC              
HindII       1081  GTCAAC               GT[CT]^[GA]AC
             1594  GTTGAC              
MaeIII       1454  GTAAC                ^GT[ACGT]AC
             1765  GTTAC               
MluNI         404  TGGCCA               TGG^CCA
             1421  TGGCCA              
MroI         1124  TCCGGA               T^CCGGA
             1398  TCCGGA              
MscI          404  TGGCCA               TGG^CCA
             1421  TGGCCA              
PstI          892  CTGCAG               CTGCA^G
             1465  CTGCAG              
SapI         1498  GAAGAGC              GCTCTTC(1/4)
             1559  GCTCTTC             
StyI          140  CCATGG               C^C[AT][AT]GG
             1531  CCTTGG              
TaqI          749  TCGA                 T^CGA
              968  TCGA                
TfiI          520  GATTC                G^A[AT]TC
              755  GATTC               
TthHB8I       749  TCGA                 T^CGA
              968  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwNI       
AspHI       AvaII       BanII       BbsI        BbvI        
BcnI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BssKI       BstF5I      BstNI       
BstUI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       EaeI        EarI        EcoO109I    
EcoRII      FauI        Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeII       MboI        
MboII       MnlI        MslI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        PpuMI       PvuII       
RsaI        SacI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        TseI        Tsp509I     
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