Restriction map for pF1KE0990
 (Circular) (Six-base) MAPSORT of: pF1KE0990.seq  Check: 4268  from: 1  to: 4988



pF1KE0990  4988 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1235    1235 
   Size:       4988
 
AatII G_ACGT'C

Cuts at:    779    1240     779 
   Size:        461    4527
 
AccI GT'mk_AC

Cuts at:    779    2026     779 
   Size:       1247    3741
 
Acc65I G'GTAC_C

Cuts at:   2004    2004 
   Size:       4988
 
AclI AA'CG_TT

Cuts at:   4828    4828 
   Size:       4988
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    599     624    1287    1399    2813    3245    3992    4260     599 
   Size:         25     663     112    1414     432     747     268    1327

  Fragments arranged by size: 

                1414    1327     747     663     432     268     112      25
            
 
AflIII A'CryG_T

Cuts at:   2340    3444    4421    2340 
   Size:       1104     977    2907

  Fragments arranged by size: 

                2907    1104     977
 
AgeI A'CCGG_T

Cuts at:   3376    3376 
   Size:       4988
 
AlwNI CAG_nnn'CTG

Cuts at:    463    1052    1759    2293    3860     463 
   Size:        589     707     534    1567    1591

  Fragments arranged by size: 

                1591    1567     707     589     534
 
ApaLI G'TGCA_C

Cuts at:   3758    3758 
   Size:       4988
 
ApoI r'AATT_y

Cuts at:   1400    1992    4341    4597    1400 
   Size:        592    2349     256    1791

  Fragments arranged by size: 

                2349    1791     592     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4988
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4988
 
AvaI C'yCGr_G

Cuts at:   2008    2008 
   Size:       4988
 
BamHI G'GATC_C

Cuts at:   2013    2013 
   Size:       4988
 
BanI G'GyrC_C

Cuts at:   1743    2004    2418    2666    2701    1743 
   Size:        261     414     248      35    4030

  Fragments arranged by size: 

                4030     414     261     248      35
 
BanII G_rGCy'C

Cuts at:    356     552    1123    1973    2002    3032     356 
   Size:        196     571     850      29    1030    2312

  Fragments arranged by size: 

                2312    1030     850     571     196      29
 
BbeI G_GCGC'C

Cuts at:   2422    2670    2422 
   Size:        248    4740
 
BbsI GAAGACnn'nnnn_

Cuts at:    804    1225    1441     804 
   Size:        421     216    4351

  Fragments arranged by size: 

                4351     421     216
 
BbvCI CC'TCA_GC

Cuts at:    476     476 
   Size:       4988
 
BciVI GTATCCnnnnn_n'

Cuts at:   2886    3647    4988    2886 
   Size:        761    1341    2886

  Fragments arranged by size: 

                2886    1341     761
 
BclI T'GATC_A

Cuts at:    212    2508     212 
   Size:       2296    2692
 
BglII A'GATC_T

Cuts at:   1466    2503    4540    1466 
   Size:       1037    2037    1914

  Fragments arranged by size: 

                2037    1914    1037
 
BlpI GC'TnA_GC

Cuts at:    175    2100     175 
   Size:       1925    3063
 
Bme1580I G_kGCm'C

Cuts at:    791    2613    2706    3762     791 
   Size:       1822      93    1056    2017

  Fragments arranged by size: 

                2017    1822    1056      93
 
BmgBI CAC'GTC

Cuts at:    714     714 
   Size:       4988
 
BmrI ACTGGGnnnn_n'

Cuts at:   2200    2281    2616    4760    2200 
   Size:         81     335    2144    2428

  Fragments arranged by size: 

                2428    2144     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:    219    1845    3355     219 
   Size:       1626    1510    1852

  Fragments arranged by size: 

                1852    1626    1510
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    370     524     553     704     720    1114    1413    1504    1876 
   Size:        154      29     151      16     394     299      91     372

Cuts at:   1876     370 
   Size:       3482

  Fragments arranged by size: 

                3482     394     372     299     154     151      91      29
                  16
 
Bpu10I CC'TnA_GC

Cuts at:    476     656    1056     476 
   Size:        180     400    4408

  Fragments arranged by size: 

                4408     400     180
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    621    2164    3043    3535    3833     621 
   Size:       1543     879     492     298    1776

  Fragments arranged by size: 

                1776    1543     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4692    4692 
   Size:       4988
 
BsaAI yAC'GTr

Cuts at:    210    2341     210 
   Size:       2131    2857
 
BsaBI GATnn'nnATC

Cuts at:      6    2502    4539       6 
   Size:       2496    2037     455

  Fragments arranged by size: 

                2496    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    776    1237    2419    2667    3369    4865     776 
   Size:        461    1182     248     702    1496     899

  Fragments arranged by size: 

                1496    1182     899     702     461     248
 
BsaWI w'CCGG_w

Cuts at:   1232    1506    2177    2698    3376    3650    3797    4208    1232 
   Size:        274     671     521     678     274     147     411    2012

  Fragments arranged by size: 

                2012     678     671     521     411     274     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    274     304     274 
   Size:         30    4958
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1381    1716    1719    1821    1824    1381 
   Size:        335       3     102       3    4545

  Fragments arranged by size: 

                4545     335     102       3       3
 
BseYI C'CCAG_C

Cuts at:    227     924    1037    2413    3171    3748    4244     227 
   Size:        697     113    1376     758     577     496     971

  Fragments arranged by size: 

                1376     971     758     697     577     496     113
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    280     280 
   Size:       4988
 
BsiEI CG_ry'CG

Cuts at:    104    2530    2576    3784     104 
   Size:       2426      46    1208    1308

  Fragments arranged by size: 

                2426    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    356    1123    1973    2002    2780    2970    3762     356 
   Size:        767     850      29     778     190     792    1582

  Fragments arranged by size: 

                1582     850     792     778     767     190      29
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1621    1621 
   Size:       4988
 
Bsp1286I G_dGCh'C

Cuts at:    356     552     791    1123    1973    2002    2613    2706    2780 
   Size:        196     239     332     850      29     611      93      74

Cuts at:   2780    2970    3032    3762     356 
   Size:        190      62     730    1582

  Fragments arranged by size: 

                1582     850     730     611     332     239     196     190
                  93      74      62      29
 
BspEI T'CCGG_A

Cuts at:   1232    1506    4208    1232 
   Size:        274    2702    2012

  Fragments arranged by size: 

                2702    2012     274
 
BspHI T'CATG_A

Cuts at:   4164    4164 
   Size:       4988
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1091    1235    2038    2252    2554    2935    1091 
   Size:        144     803     214     302     381    3144

  Fragments arranged by size: 

                3144     803     381     302     214     144
 
BsrBI CCG'CTC

Cuts at:     50     255    1611    3280    3334    4818    4983      50 
   Size:        205    1356    1669      54    1484     165      55

  Fragments arranged by size: 

                1669    1484    1356     205     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1355    2900    1355 
   Size:       1545    3443
 
BsrFI r'CCGG_y

Cuts at:    390     558    2986    3167    3376     390 
   Size:        168    2428     181     209    2002

  Fragments arranged by size: 

                2428    2002     209     181     168
 
BssSI C'ACGA_G

Cuts at:   3259    3617    3259 
   Size:        358    4630
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1759    2365    1759 
   Size:        606    4382
 
BstBI TT'CG_AA

Cuts at:   3349    3349 
   Size:       4988
 
BstXI CCAn_nnnn'nTGG

Cuts at:   1740    1740 
   Size:       4988
 
BstYI r'GATC_y

Cuts at:      1    1466    2013    2480    2503    2838    3084    4096    4182 
   Size:       1465     547     467      23     335     246    1012      86

Cuts at:   4182    4194    4540       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1465    1012     547     467     449     346     335     246
                  86      23      12
 
BtgI C'CryG_G

Cuts at:    107     248    1532     107 
   Size:        141    1284    3563

  Fragments arranged by size: 

                3563    1284     141
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2938    3119    3282    2938 
   Size:        181     163    4644

  Fragments arranged by size: 

                4644     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2231    2231 
   Size:       4988
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:   1274    1309    1274 
   Size:         35    4953
 
DraI TTT'AAA

Cuts at:   1987    1987 
   Size:       4988
 
DrdI GACnn_nn'nnGTC

Cuts at:   1004    2694    3552    1004 
   Size:       1690     858    2440

  Fragments arranged by size: 

                2440    1690     858
 
EaeI y'GGCC_r

Cuts at:    512    1322    1529    2573    2747    3138    3165    4510     512 
   Size:        810     207    1044     174     391      27    1345     990

  Fragments arranged by size: 

                1345    1044     990     810     391     207     174      27
            
 
EagI C'GGCC_G

Cuts at:   2573    2573 
   Size:       4988
 
EarI CTCTTCn'nnn_

Cuts at:    485    1601    1674    3011    3221     485 
   Size:       1116      73    1337     210    2252

  Fragments arranged by size: 

                2252    1337    1116     210      73
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3196    3506    3652    4798    3196 
   Size:        310     146    1146    3386

  Fragments arranged by size: 

                3386    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    354    1121    1971    2000     354 
   Size:        767     850      29    3342

  Fragments arranged by size: 

                3342     850     767      29
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    370     524     553     599     624     704     720    1114    1287 
   Size:        154      29      46      25      80      16     394     173

Cuts at:   1287    1399    1413    1504    1876    2813    3245    3992    4260 
   Size:        112      14      91     372     937     432     747     268

Cuts at:   4260     370 
   Size:       1098

  Fragments arranged by size: 

                1098     937     747     432     394     372     268     173
                 154     112      91      80      46      29      25      16
                  14
 
EcoNI CCTnn'n_nnAGG

Cuts at:   1486    1576    1486 
   Size:         90    4898
 
EcoO109I rG'GnC_Cy

Cuts at:    533     764    1329    1524    2127     533 
   Size:        231     565     195     603    3394

  Fragments arranged by size: 

                3394     603     565     231     195
 
EcoRI G'AATT_C

Cuts at:   1992    1992 
   Size:       4988
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3007    3007 
   Size:       4988
 
FspI TGC'GCA

Cuts at:   2769    2769 
   Size:       4988
 
HaeII r_GCGC'y

Cuts at:   2422    2670    3692    4671    2422 
   Size:        248    1022     979    2739

  Fragments arranged by size: 

                2739    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1206    1238    1283    1315    1711    1743    1819    1851    2830 
   Size:         32      45      32     396      32      76      32     979

Cuts at:   2830    2860    2862    2892    1206 
   Size:         30       2      30    3302

  Fragments arranged by size: 

                3302     979     396      76      45      32      32      32
                  32      30      30       2
 
HincII GTy'rAC

Cuts at:   1191    1704    2027    1191 
   Size:        513     323    4152

  Fragments arranged by size: 

                4152     513     323
 
KasI G'GCGC_C

Cuts at:   2418    2666    2418 
   Size:        248    4740
 
KpnI G_GTAC'C

Cuts at:   2008    2008 
   Size:       4988
 
MluI A'CGCG_T

Cuts at:   4421    4421 
   Size:       4988
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1700    3659    3843    1700 
   Size:       1959     184    2845

  Fragments arranged by size: 

                2845    1959     184
 
MscI TGG'CCA

Cuts at:    514    1531    2749     514 
   Size:       1017    1218    2753

  Fragments arranged by size: 

                2753    1218    1017
 
MslI CAynn'nnrTG

Cuts at:    908    1213    1270    1738    2977    3104     908 
   Size:        305      57     468    1239     127    2792

  Fragments arranged by size: 

                2792    1239     468     305     127      57
 
MspA1I CmG'CkG

Cuts at:    129     231    1281    1307    2099    2413    2773    3786    4031 
   Size:        102    1050      26     792     314     360    1013     245

Cuts at:   4031     129 
   Size:       1086

  Fragments arranged by size: 

                1086    1050    1013     792     360     314     245     102
                  26
 
NaeI GCC'GGC

Cuts at:    560    3169     560 
   Size:       2609    2379
 
NarI GG'CG_CC

Cuts at:   2419    2667    2419 
   Size:        248    4740
 
NcoI C'CATG_G

Cuts at:    107     248     107 
   Size:        141    4847
 
NgoMIV G'CCGG_C

Cuts at:    558    3167     558 
   Size:       2609    2379
 
NspI r_CATG'y

Cuts at:    306    2041    2541    3448     306 
   Size:       1735     500     907    1846

  Fragments arranged by size: 

                1846    1735     907     500
 
PasI CC'CwG_GG

Cuts at:    529    1748     529 
   Size:       1219    3769
 
PciI A'CATG_T

Cuts at:   3444    3444 
   Size:       4988
 
PfoI T'CCnGG_A

Cuts at:    846    1429     846 
   Size:        583    4405
 
PmeI GTTT'AAAC

Cuts at:   1987    1987 
   Size:       4988
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4127    4159    4127 
   Size:         32    4956
 
PpuMI rG'GwC_Cy

Cuts at:    533     764    1329     533 
   Size:        231     565    4192

  Fragments arranged by size: 

                4192     565     231
 
PstI C_TGCA'G

Cuts at:   1004    1577    2035    2720    1004 
   Size:        573     458     685    3272

  Fragments arranged by size: 

                3272     685     573     458
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4988
 
PvuII CAG'CTG

Cuts at:    129     231    1281    1307    2413    2773     129 
   Size:        102    1050      26    1106     360    2344

  Fragments arranged by size: 

                2344    1106    1050     360     102      26
 
RsrII CG'GwC_CG

Cuts at:   3183    3183 
   Size:       4988
 
SacI G_AGCT'C

Cuts at:    356    1123    1973    2002     356 
   Size:        767     850      29    3342

  Fragments arranged by size: 

                3342     850     767      29
 
SalI G'TCGA_C

Cuts at:   2025    2025 
   Size:       4988
 
SapI GCTCTTCn'nnn_

Cuts at:   1601    1674    3011    3221    1601 
   Size:         73    1337     210    3368

  Fragments arranged by size: 

                3368    1337     210      73
 
SbfI CC_TGCA'GG

Cuts at:   1577    2035    1577 
   Size:        458    4530
 
ScaI AGT'ACT

Cuts at:   4505    4505 
   Size:       4988
 
SfcI C'TryA_G

Cuts at:     33     780     988    1000    1573    2031    2716    3709    3900 
   Size:        747     208      12     573     458     685     993     191

Cuts at:   3900      33 
   Size:       1121

  Fragments arranged by size: 

                1121     993     747     685     573     458     208     191
                  12
 
SfoI GGC'GCC

Cuts at:   2420    2668    2420 
   Size:        248    4740
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4988
 
SmaI CCC'GGG

Cuts at:   2010    2010 
   Size:       4988
 
SmlI C'TyrA_G

Cuts at:    600    2143    3058    3550    3812     600 
   Size:       1543     915     492     262    1776

  Fragments arranged by size: 

                1776    1543     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4988
 
SphI G_CATG'C

Cuts at:    306    2041    2541     306 
   Size:       1735     500    2753

  Fragments arranged by size: 

                2753    1735     500
 
SspI AAT'ATT

Cuts at:   1932    1932 
   Size:       4988
 
StuI AGG'CCT

Cuts at:   1843    1843 
   Size:       4988
 
StyI C'CwwG_G

Cuts at:    107     248    1639    2122    2476     107 
   Size:        141    1391     483     354    2619

  Fragments arranged by size: 

                2619    1391     483     354     141
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    129    1181    2571    2704    3163    3372     129 
   Size:       1052    1390     133     459     209    1745

  Fragments arranged by size: 

                1745    1390    1052     459     209     133
 
TatI w'GTAC_w

Cuts at:   4503    4503 
   Size:       4988
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    139     171     274     306     325     357     636     668    4505 
   Size:         32     103      32      19      32     279      32    3837

Cuts at:   4505    4537     139 
   Size:         32     590

  Fragments arranged by size: 

                3837     590     279     103      32      32      32      32
                  32      19
 
Tth111I GACn'n_nGTC

Cuts at:    715    2785     715 
   Size:       2070    2918
 
XbaI T'CTAG_A

Cuts at:   2019    2019 
   Size:       4988
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2483    2483 
   Size:       4988
 
XmaI C'CCGG_G

Cuts at:   2008    2008 
   Size:       4988
 
XmnI GAAnn'nnTTC

Cuts at:    805     805 
   Size:       4988
 
ZraI GAC'GTC

Cuts at:    777    1238     777 
   Size:        461    4527
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI    BciVI     BclI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstXI    BstYI     BtgI
    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI     FspI
    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI
     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PasI
     PciI     PfoI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII
    RsrII     SacI     SalI     SapI     SbfI     ScaI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI     AscI
    AvrII     BaeI     BcgI    BfrBI     BglI     BmtI    BsiWI     BsmI
    BsrGI   BssHII   BstEII  BstZ17I   Bsu36I     ClaI   DraIII    EcoRV
     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI
     NruI     NsiI     PacI    PflMI     PmlI    PshAI     PsiI   PspOMI
    PspXI     PsrI    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI
     SrfI     SwaI     XhoI
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