Restriction map for pF1KE0990
 (Linear) (Six-base) MAPSORT of: KIEE0990.seq  Check: 2404  from: 1  to: 1875



KIEE0990.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:31  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1127    1875 
   Size:       1127     748
 
AatII G_ACGT'C

Cuts at:      0     671    1132    1875 
   Size:        671     461     743

  Fragments arranged by size: 

                 743     671     461
 
AccI GT'mk_AC

Cuts at:      0     671    1875 
   Size:        671    1204
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     491     516    1179    1291    1875 
   Size:        491      25     663     112     584

  Fragments arranged by size: 

                 663     584     491     112      25
 
AlwNI CAG_nnn'CTG

Cuts at:      0     355     944    1651    1875 
   Size:        355     589     707     224

  Fragments arranged by size: 

                 707     589     355     224
 
ApoI r'AATT_y

Cuts at:      0    1292    1875 
   Size:       1292     583
 
BanI G'GyrC_C

Cuts at:      0    1635    1875 
   Size:       1635     240
 
BanII G_rGCy'C

Cuts at:      0     248     444    1015    1865    1875 
   Size:        248     196     571     850      10

  Fragments arranged by size: 

                 850     571     248     196      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     696    1117    1333    1875 
   Size:        696     421     216     542

  Fragments arranged by size: 

                 696     542     421     216
 
BbvCI CC'TCA_GC

Cuts at:      0     368    1875 
   Size:        368    1507
 
BclI T'GATC_A

Cuts at:      0     104    1875 
   Size:        104    1771
 
BglII A'GATC_T

Cuts at:      0    1358    1875 
   Size:       1358     517
 
BlpI GC'TnA_GC

Cuts at:      0      67    1875 
   Size:         67    1808
 
Bme1580I G_kGCm'C

Cuts at:      0     683    1875 
   Size:        683    1192
 
BmgBI CAC'GTC

Cuts at:      0     606    1875 
   Size:        606    1269
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     111    1737    1875 
   Size:        111    1626     138

  Fragments arranged by size: 

                1626     138     111
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     416     445     596     612    1006    1305    1396 
   Size:        262     154      29     151      16     394     299      91

Cuts at:   1396    1768    1875 
   Size:        372     107

  Fragments arranged by size: 

                 394     372     299     262     154     151     107      91
                  29      16
 
Bpu10I CC'TnA_GC

Cuts at:      0     368     548     948    1875 
   Size:        368     180     400     927

  Fragments arranged by size: 

                 927     400     368     180
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     513    1875 
   Size:        513    1362
 
BsaAI yAC'GTr

Cuts at:      0     102    1875 
   Size:        102    1773
 
BsaHI Gr'CG_yC

Cuts at:      0     668    1129    1875 
   Size:        668     461     746

  Fragments arranged by size: 

                 746     668     461
 
BsaWI w'CCGG_w

Cuts at:      0    1124    1398    1875 
   Size:       1124     274     477

  Fragments arranged by size: 

                1124     477     274
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     166     196    1875 
   Size:        166      30    1679

  Fragments arranged by size: 

                1679     166      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1273    1608    1611    1713    1716    1875 
   Size:       1273     335       3     102       3     159

  Fragments arranged by size: 

                1273     335     159     102       3       3
 
BseYI C'CCAG_C

Cuts at:      0     119     816     929    1875 
   Size:        119     697     113     946

  Fragments arranged by size: 

                 946     697     119     113
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172    1875 
   Size:        172    1703
 
BsiHKAI G_wGCw'C

Cuts at:      0     248    1015    1865    1875 
   Size:        248     767     850      10

  Fragments arranged by size: 

                 850     767     248      10
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1513    1875 
   Size:       1513     362
 
Bsp1286I G_dGCh'C

Cuts at:      0     248     444     683    1015    1865    1875 
   Size:        248     196     239     332     850      10

  Fragments arranged by size: 

                 850     332     248     239     196      10
 
BspEI T'CCGG_A

Cuts at:      0    1124    1398    1875 
   Size:       1124     274     477

  Fragments arranged by size: 

                1124     477     274
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     983    1127    1875 
   Size:        983     144     748

  Fragments arranged by size: 

                 983     748     144
 
BsrBI CCG'CTC

Cuts at:      0     147    1503    1875 
   Size:        147    1356     372

  Fragments arranged by size: 

                1356     372     147
 
BsrDI GCAATG_nn'

Cuts at:      0    1247    1875 
   Size:       1247     628
 
BsrFI r'CCGG_y

Cuts at:      0     282     450    1875 
   Size:        282     168    1425

  Fragments arranged by size: 

                1425     282     168
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1651    1875 
   Size:       1651     224
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1632    1875 
   Size:       1632     243
 
BstYI r'GATC_y

Cuts at:      0    1358    1875 
   Size:       1358     517
 
BtgI C'CryG_G

Cuts at:      0     140    1424    1875 
   Size:        140    1284     451

  Fragments arranged by size: 

                1284     451     140
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1166    1201    1875 
   Size:       1166      35     674

  Fragments arranged by size: 

                1166     674      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     896    1875 
   Size:        896     979
 
EaeI y'GGCC_r

Cuts at:      0     404    1214    1421    1875 
   Size:        404     810     207     454

  Fragments arranged by size: 

                 810     454     404     207
 
EarI CTCTTCn'nnn_

Cuts at:      0     377    1493    1566    1875 
   Size:        377    1116      73     309

  Fragments arranged by size: 

                1116     377     309      73
 
EcoICRI GAG'CTC

Cuts at:      0     246    1013    1863    1875 
   Size:        246     767     850      12

  Fragments arranged by size: 

                 850     767     246      12
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     262     416     445     491     516     596     612    1006 
   Size:        262     154      29      46      25      80      16     394

Cuts at:   1006    1179    1291    1305    1396    1768    1875 
   Size:        173     112      14      91     372     107

  Fragments arranged by size: 

                 394     372     262     173     154     112     107      91
                  80      46      29      25      16      14
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1378    1468    1875 
   Size:       1378      90     407

  Fragments arranged by size: 

                1378     407      90
 
EcoO109I rG'GnC_Cy

Cuts at:      0     425     656    1221    1416    1875 
   Size:        425     231     565     195     459

  Fragments arranged by size: 

                 565     459     425     231     195
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1098    1130    1175    1207    1603    1635    1711    1743 
   Size:       1098      32      45      32     396      32      76      32

Cuts at:   1743    1875 
   Size:        132

  Fragments arranged by size: 

                1098     396     132      76      45      32      32      32
                  32
 
HincII GTy'rAC

Cuts at:      0    1083    1596    1875 
   Size:       1083     513     279

  Fragments arranged by size: 

                1083     513     279
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1592    1875 
   Size:       1592     283
 
MscI TGG'CCA

Cuts at:      0     406    1423    1875 
   Size:        406    1017     452

  Fragments arranged by size: 

                1017     452     406
 
MslI CAynn'nnrTG

Cuts at:      0     800    1105    1162    1630    1875 
   Size:        800     305      57     468     245

  Fragments arranged by size: 

                 800     468     305     245      57
 
MspA1I CmG'CkG

Cuts at:      0      21     123    1173    1199    1875 
   Size:         21     102    1050      26     676

  Fragments arranged by size: 

                1050     676     102      26      21
 
NaeI GCC'GGC

Cuts at:      0     452    1875 
   Size:        452    1423
 
NcoI C'CATG_G

Cuts at:      0     140    1875 
   Size:        140    1735
 
NgoMIV G'CCGG_C

Cuts at:      0     450    1875 
   Size:        450    1425
 
NspI r_CATG'y

Cuts at:      0     198    1875 
   Size:        198    1677
 
PasI CC'CwG_GG

Cuts at:      0     421    1640    1875 
   Size:        421    1219     235

  Fragments arranged by size: 

                1219     421     235
 
PfoI T'CCnGG_A

Cuts at:      0     738    1321    1875 
   Size:        738     583     554

  Fragments arranged by size: 

                 738     583     554
 
PpuMI rG'GwC_Cy

Cuts at:      0     425     656    1221    1875 
   Size:        425     231     565     654

  Fragments arranged by size: 

                 654     565     425     231
 
PstI C_TGCA'G

Cuts at:      0     896    1469    1875 
   Size:        896     573     406

  Fragments arranged by size: 

                 896     573     406
 
PvuII CAG'CTG

Cuts at:      0      21     123    1173    1199    1875 
   Size:         21     102    1050      26     676

  Fragments arranged by size: 

                1050     676     102      26      21
 
SacI G_AGCT'C

Cuts at:      0     248    1015    1865    1875 
   Size:        248     767     850      10

  Fragments arranged by size: 

                 850     767     248      10
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1493    1566    1875 
   Size:       1493      73     309

  Fragments arranged by size: 

                1493     309      73
 
SbfI CC_TGCA'GG

Cuts at:      0    1469    1875 
   Size:       1469     406
 
SfcI C'TryA_G

Cuts at:      0     672     880     892    1465    1875 
   Size:        672     208      12     573     410

  Fragments arranged by size: 

                 672     573     410     208      12
 
SmlI C'TyrA_G

Cuts at:      0     492    1875 
   Size:        492    1383
 
SphI G_CATG'C

Cuts at:      0     198    1875 
   Size:        198    1677
 
SspI AAT'ATT

Cuts at:      0    1824    1875 
   Size:       1824      51
 
StuI AGG'CCT

Cuts at:      0    1735    1875 
   Size:       1735     140
 
StyI C'CwwG_G

Cuts at:      0     140    1531    1875 
   Size:        140    1391     344

  Fragments arranged by size: 

                1391     344     140
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      21    1073    1875 
   Size:         21    1052     802

  Fragments arranged by size: 

                1052     802      21
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      31      63     166     198     217     249     528     560 
   Size:         31      32     103      32      19      32     279      32

Cuts at:    560    1875 
   Size:       1315

  Fragments arranged by size: 

                1315     279     103      32      32      32      32      31
                  19
 
Tth111I GACn'n_nGTC

Cuts at:      0     607    1875 
   Size:        607    1268
 
XmnI GAAnn'nnTTC

Cuts at:      0     697    1875 
   Size:        697    1178
 
ZraI GAC'GTC

Cuts at:      0     669    1130    1875 
   Size:        669     461     745

  Fragments arranged by size: 

                 745     669     461
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI    AlwNI     ApoI     BanI    BanII
     BbsI    BbvCI     BclI    BglII     BlpI Bme1580I    BmgBI     BplI
     BpmI   Bpu10I    BpuEI    BsaAI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspEI    BspMI    BsrBI
    BsrDI    BsrFI   BstAPI    BstXI    BstYI     BtgI    CspCI     DrdI
     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I    Hin4I   HincII
     MmeI     MscI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NspI
     PasI     PfoI    PpuMI     PstI    PvuII     SacI     SapI     SbfI
     SfcI     SmlI     SphI     SspI     StuI     StyI    TaqII     TstI
  Tth111I     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI     AleI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BcgI    BciVI    BfrBI     BglI
     BmrI     BmtI     BsaI    BsaBI    BsiEI    BsiWI     BsmI    BspHI
    BsrGI   BssHII    BssSI    BstBI   BstEII  BstZ17I   Bsu36I    BtgZI
     BtsI     ClaI     DraI   DraIII     EagI     EciI    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     NarI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PciI    PflMI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
    SnaBI     SpeI     SrfI     SwaI     TatI     XbaI     XcmI     XhoI
     XmaI
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