Restriction map for pF1KB8500
##################################
# NO CUT ENZYMES (  104 enzymes) #
##################################

Acc65I      AccII       AccIII      AclI        AgeI        
AhdI        AocI        Aor51HI     ApaI        AscI        
Asp718I     AspEI       AspI        AviII       AvrII       
BalI        BamHI       BbeI        BbrPI       BcnI        
BglI        BlnI        BsaAI       BsaBI       BsaI        
BseAI       BseRI       BsiEI       BsiWI       BsmBI       
Bsp1407I    BspEI       BsrDI       BsrGI       BssHII      
Bst1107I    BstEII      BstPI       BstUI       BstXI       
BstZ17I     Bsu36I      CpoI        DraII       EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoO109I    EcoO65I     EcoRI       FseI        FspI        
HpaI        KasI        KpnI        KspI        MamI        
MfeI        MluI        MluNI       MroI        MscI        
MunI        MvnI        NarI        NciI        NdeI        
NheI        NotI        NruI        PacI        PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       Psp1406I    PvuI        RsrII       SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
Sse8387I    SspBI       SwaI        Tth111I     Van91I      
XbaI        XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   46 enzymes) #
###################################

AatII        2344  GACGTC               GACGT^C
AccI          925  GTCTAC               GT^[AC][GT]AC
AflII         172  CTTAAG               C^TTAAG
Alw44I        344  GTGCAC               G^TGCAC
ApaLI         344  GTGCAC               G^TGCAC
AseI         1831  ATTAAT               AT^TAAT
AsnI         1831  ATTAAT               AT^TAAT
BbsI         1362  GTCTTC               GAAGAC(2/6)
BbvCI        1696  CCTCAGC              CCTCAGC(-5/-2)
BcgI         2265  CGATGAAAATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI         951  GTATCC               GTATCC(6/5)
BfrI          172  CTTAAG               C^TTAAG
BglII        1405  AGATCT               A^GATCT
BlpI         2367  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I     2367  GCTGAGC              GC^T[ACGT]AGC
BpuAI        1362  GTCTTC               GAAGAC(2/6)
BsaWI        1672  ACCGGA               [AT]^CCGG[AT]
BsgI          475  GTGCAG               GTGCAG(16/14)
BsmAI        2550  GAGAC                GTCTC(1/5)
BsmI          988  GAATGC               GAATGC(1/-1)
BspDI        2263  ATCGAT               AT^CGAT
BspHI        1899  TCATGA               T^CATGA
BsrBI        1460  GAGCGG               CCGCTC(-3/-3)
BssSI         913  CTCGTG               CACGAG(-5/-1)
BstBI         238  TTCGAA               TT^CGAA
CelII        2367  GCTGAGC              GC^T[ACGT]AGC
ClaI         2263  ATCGAT               AT^CGAT
DrdI         1390  GACAAAGGCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI         1559  CGGCCA               [CT]^GGCC[GA]
EcoNI         291  CCTAACTCAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI          449  CCCGC                CCCGC(4/6)
HaeII        1614  AGCGCC               [GA]GCGC^[CT]
HgaI         1397  GCGTC                GACGC(5/10)
HincII       1099  GTCAAC               GT[CT]^[GA]AC
HindII       1099  GTCAAC               GT[CT]^[GA]AC
NaeI         2393  GCCGGC               GCC^GGC
NgoMIV       2393  GCCGGC               G^CCGGC
NspV          238  TTCGAA               TT^CGAA
PaeR7I         18  CTCGAG               C^TCGAG
PshBI        1831  ATTAAT               AT^TAAT
PvuII         730  CAGCTG               CAG^CTG
RcaI         1899  TCATGA               T^CATGA
SfuI          238  TTCGAA               TT^CGAA
SpeI         2314  ACTAGT               A^CTAGT
StuI         1265  AGGCCT               AGG^CCT
XhoI           18  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   33 enzymes) #
###################################

AcyI         1396  GGCGTC               G[GA]^CG[CT]C
             2344  GACGTC              
AlwI          189  GGATC                GGATC(4/5)
             1200  GGATC               
BanI          148  GGTGCC               G^G[CT][GA]CC
             1378  GGTGCC              
BclI          411  TGATCA               T^GATCA
             2489  TGATCA              
BsaHI        1396  GGCGTC               G[GA]^CG[CT]C
             2344  GACGTC              
BsrFI         878  ACCGGC               [GA]^CCGG[CT]
             2393  GCCGGC              
BstYI         189  GGATCT               [GA]^GATC[CT]
             1405  AGATCT              
CfoI          504  GCGC                 GCG^C
             1615  GCGC                
Cfr10I        878  ACCGGC               [GA]^CCGG[CT]
             2393  GCCGGC              
DraI         1062  TTTAAA               TTT^AAA
             1735  TTTAAA              
DraIII        338  CACTTTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2143  CACACCGTG           
EarI         1274  CTCTTC               CTCTTC(1/4)
              757  GAAGAG              
EcoRV         997  GATATC               GAT^ATC
             2080  GATATC              
EcoT14I      1184  CCATGG               C^C[AT][AT]GG
             2532  CCATGG              
EcoT22I       566  ATGCAT               ATGCA^T
             1159  ATGCAT              
FbaI          411  TGATCA               T^GATCA
             2489  TGATCA              
HhaI          504  GCGC                 GCG^C
             1615  GCGC                
Hin1I        1396  GGCGTC               G[GA]^CG[CT]C
             2344  GACGTC              
HinP1I        504  GCGC                 G^CGC
             1615  GCGC                
HindIII       169  AAGCTT               A^AGCTT
             1045  AAGCTT              
Ksp632I      1274  CTCTTC               CTCTTC(1/4)
              757  GAAGAG              
MaeII        1241  ACGT                 A^CGT
             2345  ACGT                
MflI          189  GGATCT               [GA]^GATC[CT]
             1405  AGATCT              
MslI         1494  CACTTCCGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2493  CACCCACATG          
MspA1I        730  CAGCTG               C[AC]G^C[GT]G
             1166  CAGCGG              
NcoI         1184  CCATGG               C^CATGG
             2532  CCATGG              
NsiI          566  ATGCAT               ATGCA^T
             1159  ATGCAT              
PshAI         462  GACCACAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             1765  GACATTTGTC          
SacI           15  GAGCTC               GAGCT^C
              333  GAGCTC              
SmlI           18  CTCGAG               C^T[CT][GA]AG
              172  CTTAAG              
SphI         1161  GCATGC               GCATG^C
             2100  GCATGC              
StyI         1184  CCATGG               C^C[AT][AT]GG
             2532  CCATGG              
XhoII         189  GGATCT               [GA]^GATC[CT]
             1405  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   75 enzymes) #
#########################################

AciI        AcsI        AfaI        AflIII      AluI        
AlwNI       ApoI        Asp700I     AspHI       AvaI        
AvaII       BanII       BbvI        BfaI        BmyI        
BpmI        BsaJI       BsiHKAI     BsiYI       BslI        
BsmFI       BsoBI       Bsp1286I    BspLU11I    BspMI       
BsrI        BssKI       BstAPI      BstF5I      BstNI       
Cac8I       Cfr13I      DdeI        DpnII       DsaI        
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HinfI       HpaII       HphI        ItaI        MaeI        
MaeIII      MboI        MboII       MnlI        MseI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       NspI        PleI        PstI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SspI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     XmnI        
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