Restriction map for pF1KB8500
 (Circular) (Six-base) MAPSORT of: pF1KB8500.seq  Check: 4456  from: 1  to: 5699



pF1KB8500  5699 bp

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    186     186 
   Size:       5699
 
AatII G_ACGT'C

Cuts at:   2456    2456 
   Size:       5699
 
AccI GT'mk_AC

Cuts at:   1034    2737    1034 
   Size:       1703    3996
 
Acc65I G'GTAC_C

Cuts at:   2715    2715 
   Size:       5699
 
AclI AA'CG_TT

Cuts at:   2693    5539    2693 
   Size:       2846    2853
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1466    3524    3956    4703    4971    1466 
   Size:       2058     432     747     268    2194

  Fragments arranged by size: 

                2194    2058     747     432     268
 
AflII C'TTAA_G

Cuts at:    280     280 
   Size:       5699
 
AflIII A'CryG_T

Cuts at:   1040    1349    1701    1904    3051    4155    5132    1040 
   Size:        309     352     203    1147    1104     977    1607

  Fragments arranged by size: 

                1607    1147    1104     977     352     309     203
 
AgeI A'CCGG_T

Cuts at:   4087    4087 
   Size:       5699
 
AleI CACnn'nnGTG

Cuts at:   1606    1606 
   Size:       5699
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1029    2543    1029 
   Size:       1514    4185
 
AlwNI CAG_nnn'CTG

Cuts at:    139    1236    1649    2392    2540    3004    4571     139 
   Size:       1097     413     743     148     464    1567    1267

  Fragments arranged by size: 

                1567    1267    1097     743     464     413     148
 
ApaLI G'TGCA_C

Cuts at:    452    4469     452 
   Size:       4017    1682
 
ApoI r'AATT_y

Cuts at:    622    1133    1789    1856    2703    5052    5308     622 
   Size:        511     656      67     847    2349     256    1013

  Fragments arranged by size: 

                2349    1013     847     656     511     256      67
 
AseI AT'TA_AT

Cuts at:     20    1940      20 
   Size:       1920    3779
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5699
 
AvaI C'yCGr_G

Cuts at:    126    1587    2510    2719     126 
   Size:       1461     923     209    3106

  Fragments arranged by size: 

                3106    1461     923     209
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   1042    1075    1042 
   Size:         33    5666
 
BamHI G'GATC_C

Cuts at:   2724    2724 
   Size:       5699
 
BanI G'GyrC_C

Cuts at:    256    1486    2715    3129    3377    3412     256 
   Size:       1230    1229     414     248      35    2543

  Fragments arranged by size: 

                2543    1230    1229     414     248      35
 
BanII G_rGCy'C

Cuts at:    127     445    1444    1693    2713    3743     127 
   Size:        318     999     249    1020    1030    2083

  Fragments arranged by size: 

                2083    1030    1020     999     318     249
 
BbeI G_GCGC'C

Cuts at:   3133    3381    3133 
   Size:        248    5451
 
BbsI GAAGACnn'nnnn_

Cuts at:   1463    1463 
   Size:       5699
 
BbvCI CC'TCA_GC

Cuts at:   1805    1805 
   Size:       5699
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   2362    2396    2362 
   Size:         34    5665
 
BciVI GTATCCnnnnn_n'

Cuts at:   1070    3597    4358    5699    1070 
   Size:       2527     761    1341    1070

  Fragments arranged by size: 

                2527    1341    1070     761
 
BclI T'GATC_A

Cuts at:    519    2597    3219     519 
   Size:       2078     622    2999

  Fragments arranged by size: 

                2999    2078     622
 
BfrBI ATG'CAT

Cuts at:    676    1269     676 
   Size:        593    5106
 
BglII A'GATC_T

Cuts at:   1513    3214    5251    1513 
   Size:       1701    2037    1961

  Fragments arranged by size: 

                2037    1961    1701
 
BlpI GC'TnA_GC

Cuts at:   2476    2811    2476 
   Size:        335    5364
 
Bme1580I G_kGCm'C

Cuts at:    456    1595    1958    3324    3417    4473     456 
   Size:       1139     363    1366      93    1056    1682

  Fragments arranged by size: 

                1682    1366    1139    1056     363      93
 
BmgBI CAC'GTC

Cuts at:   1350    1350 
   Size:       5699
 
BmrI ACTGGGnnnn_n'

Cuts at:   1544    2911    2992    3327    5471    1544 
   Size:       1367      81     335    2144    1772

  Fragments arranged by size: 

                2144    1772    1367     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1602    4066    1602 
   Size:       2464    3235
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1103    1352    1409    2090    2245    2369    2517    1103 
   Size:        249      57     681     155     124     148    4285

  Fragments arranged by size: 

                4285     681     249     155     148     124      57
 
Bpu10I CC'TnA_GC

Cuts at:    143    1805     143 
   Size:       1662    4037
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2875    3754    4246    4544    2875 
   Size:        879     492     298    4030

  Fragments arranged by size: 

                4030     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   5403    5403 
   Size:       5699
 
BsaAI yAC'GTr

Cuts at:   3052    3052 
   Size:       5699
 
BsaBI GATnn'nnATC

Cuts at:      6    3213    5250       6 
   Size:       3207    2037     455

  Fragments arranged by size: 

                3207    2037     455
 
BsaHI Gr'CG_yC

Cuts at:   1505    2453    3130    3378    4080    5576    1505 
   Size:        948     677     248     702    1496    1628

  Fragments arranged by size: 

                1628    1496     948     702     677     248
 
BsaWI w'CCGG_w

Cuts at:   1780    2888    3409    4087    4361    4508    4919    1780 
   Size:       1108     521     678     274     147     411    2560

  Fragments arranged by size: 

                2560    1108     678     521     411     274     147
 
BseYI C'CCAG_C

Cuts at:    769    1002    3124    3882    4459    4955     769 
   Size:        233    2122     758     577     496    1513

  Fragments arranged by size: 

                2122    1513     758     577     496     233
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    604     604 
   Size:       5699
 
BsiEI CG_ry'CG

Cuts at:    104    3241    3287    4495     104 
   Size:       3137      46    1208    1308

  Fragments arranged by size: 

                3137    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    127     445     456    2261    2713    3491    3681    4473     127 
   Size:        318      11    1805     452     778     190     792    1353

  Fragments arranged by size: 

                1805    1353     792     778     452     318     190      11
            
 
BsmI GAATG_Cn'

Cuts at:   1102    1102 
   Size:       5699
 
Bsp1286I G_dGCh'C

Cuts at:    127     445     456    1444    1595    1693    1958    2261    2713 
   Size:        318      11     988     151      98     265     303     452

Cuts at:   2713    3324    3417    3491    3681    3743    4473     127 
   Size:        611      93      74     190      62     730    1353

  Fragments arranged by size: 

                1353     988     730     611     452     318     303     265
                 190     151      98      93      74      62      11
 
BspEI T'CCGG_A

Cuts at:   4919    4919 
   Size:       5699
 
BspHI T'CATG_A

Cuts at:   2007    4875    2007 
   Size:       2868    2831
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    147     186     487    2749    2963    3265    3646     147 
   Size:         39     301    2262     214     302     381    2200

  Fragments arranged by size: 

                2262    2200     381     302     301     214      39
 
BsrBI CCG'CTC

Cuts at:     50    1570    3991    4045    5529    5694      50 
   Size:       1520    2421      54    1484     165      55

  Fragments arranged by size: 

                2421    1520    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   3611    3611 
   Size:       5699
 
BsrFI r'CCGG_y

Cuts at:    986    2501    3697    3878    4087     986 
   Size:       1515    1196     181     209    2598

  Fragments arranged by size: 

                2598    1515    1196     209     181
 
BssSI C'ACGA_G

Cuts at:   1021    3970    4328    1021 
   Size:       2949     358    2392

  Fragments arranged by size: 

                2949    2392     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1137    1236    1649    2256    3076    1137 
   Size:         99     413     607     820    3760

  Fragments arranged by size: 

                3760     820     607     413      99
 
BstBI TT'CG_AA

Cuts at:    347    4060     347 
   Size:       3713    1986
 
BstYI r'GATC_y

Cuts at:      1     297    1513    2724    3191    3214    3549    3795    4807 
   Size:        296    1216    1211     467      23     335     246    1012

Cuts at:   4807    4893    4905    5251       1 
   Size:         86      12     346     449

  Fragments arranged by size: 

                1216    1211    1012     467     449     346     335     296
                 246      86      23      12
 
BtgI C'CryG_G

Cuts at:    107    1292    1607    2213    2640     107 
   Size:       1185     315     606     427    3166

  Fragments arranged by size: 

                3166    1185     606     427     315
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    476    2649    3649    3830    3993     476 
   Size:       2173    1000     181     163    2182

  Fragments arranged by size: 

                2182    2173    1000     181     163
 
BtsI GCAGTG_nn'

Cuts at:    215    1234    1535    1647    2942     215 
   Size:       1019     301     112    1295    2972

  Fragments arranged by size: 

                2972    1295    1019     301     112
 
ClaI AT'CG_AT

Cuts at:   2372    2372 
   Size:       5699
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    249     284     306     341     249 
   Size:         35      22      35    5607

  Fragments arranged by size: 

                5607      35      35      22
 
DraI TTT'AAA

Cuts at:   1172    1845    2698    1172 
   Size:        673     853    4173

  Fragments arranged by size: 

                4173     853     673
 
DraIII CAC_nnn'GTG

Cuts at:    451    2256     451 
   Size:       1805    3894
 
DrdI GACnn_nn'nnGTC

Cuts at:   1504    3405    4263    1504 
   Size:       1901     858    2940

  Fragments arranged by size: 

                2940    1901     858
 
EaeI y'GGCC_r

Cuts at:   1667    3284    3458    3849    3876    5221    1667 
   Size:       1617     174     391      27    1345    2145

  Fragments arranged by size: 

                2145    1617    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   3284    3284 
   Size:       5699
 
EarI CTCTTCn'nnn_

Cuts at:    860    1388    3722    3932     860 
   Size:        528    2334     210    2627

  Fragments arranged by size: 

                2627    2334     528     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   3907    4217    4363    5509    3907 
   Size:        310     146    1146    4097

  Fragments arranged by size: 

                4097    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    125     443    2711     125 
   Size:        318    2268    3113

  Fragments arranged by size: 

                3113    2268     318
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1103    1352    1409    1466    2090    2245    2369    2517    3524 
   Size:        249      57      57     624     155     124     148    1007

Cuts at:   3524    3956    4703    4971    1103 
   Size:        432     747     268    1831

  Fragments arranged by size: 

                1831    1007     747     624     432     268     249     155
                 148     124      57      57
 
EcoNI CCTnn'n_nnAGG

Cuts at:    403     403 
   Size:       5699
 
EcoO109I rG'GnC_Cy

Cuts at:   2838    2838 
   Size:       5699
 
EcoRI G'AATT_C

Cuts at:   2703    2703 
   Size:       5699
 
EcoRV GAT'ATC

Cuts at:   1107    2190    1107 
   Size:       1083    4616
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   1876    2247    2373    3718    1876 
   Size:        371     126    1345    3857

  Fragments arranged by size: 

                3857    1345     371     126
 
FspI TGC'GCA

Cuts at:   3480    3480 
   Size:       5699
 
HaeII r_GCGC'y

Cuts at:   1726    3133    3381    4403    5382    1726 
   Size:       1407     248    1022     979    2043

  Fragments arranged by size: 

                2043    1407    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    154     186     504     536    1524    1556    1675    1707    2316 
   Size:         32     318      32     988      32     119      32     609

Cuts at:   2316    2348    3541    3571    3573    3603     154 
   Size:         32    1193      30       2      30    2250

  Fragments arranged by size: 

                2250    1193     988     609     318     119      32      32
                  32      32      32      30      30       2
 
HincII GTy'rAC

Cuts at:   1209    2738    1209 
   Size:       1529    4170
 
HindIII A'AGCT_T

Cuts at:    277    1153     277 
   Size:        876    4823
 
KasI G'GCGC_C

Cuts at:   3129    3377    3129 
   Size:        248    5451
 
KpnI G_GTAC'C

Cuts at:   2719    2719 
   Size:       5699
 
MluI A'CGCG_T

Cuts at:   5132    5132 
   Size:       5699
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    480     927    4370    4554     480 
   Size:        447    3443     184    1625

  Fragments arranged by size: 

                3443    1625     447     184
 
MscI TGG'CCA

Cuts at:   3460    3460 
   Size:       5699
 
MslI CAynn'nnrTG

Cuts at:   1606    2605    3688    3815    1606 
   Size:        999    1083     127    3490

  Fragments arranged by size: 

                3490    1083     999     127
 
MspA1I CmG'CkG

Cuts at:    840    1276    2810    3124    3484    4497    4742     840 
   Size:        436    1534     314     360    1013     245    1797

  Fragments arranged by size: 

                1797    1534    1013     436     360     314     245
 
NaeI GCC'GGC

Cuts at:   2503    3880    2503 
   Size:       1377    4322
 
NarI GG'CG_CC

Cuts at:   3130    3378    3130 
   Size:        248    5451
 
NcoI C'CATG_G

Cuts at:    107    1292    2640     107 
   Size:       1185    1348    3166

  Fragments arranged by size: 

                3166    1348    1185
 
NgoMIV G'CCGG_C

Cuts at:   2501    3878    2501 
   Size:       1377    4322
 
NsiI A_TGCA'T

Cuts at:    678    1271     678 
   Size:        593    5106
 
NspI r_CATG'y

Cuts at:    676    1044    1273    1705    1908    2212    2752    3252    4159 
   Size:        368     229     432     203     304     540     500     907

Cuts at:   4159     676 
   Size:       2216

  Fragments arranged by size: 

                2216     907     540     500     432     368     304     229
                 203
 
PasI CC'CwG_GG

Cuts at:   1678    2488    1678 
   Size:        810    4889
 
PciI A'CATG_T

Cuts at:   1040    1701    1904    4155    1040 
   Size:        661     203    2251    2584

  Fragments arranged by size: 

                2584    2251     661     203
 
PfoI T'CCnGG_A

Cuts at:   2067    2067 
   Size:       5699
 
PmeI GTTT'AAAC

Cuts at:   2698    2698 
   Size:       5699
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   4838    4870    4838 
   Size:         32    5667
 
PshAI GACnn'nnGTC

Cuts at:    574    1877     574 
   Size:       1303    4396
 
PsiI TTA'TAA

Cuts at:   2167    2167 
   Size:       5699
 
PspXI vC'TCGA_Gb

Cuts at:    126     126 
   Size:       5699
 
PstI C_TGCA'G

Cuts at:   1018    1654    2246    2746    3431    1018 
   Size:        636     592     500     685    3286

  Fragments arranged by size: 

                3286     685     636     592     500
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       5699
 
PvuII CAG'CTG

Cuts at:    840    3124    3484     840 
   Size:       2284     360    3055

  Fragments arranged by size: 

                3055    2284     360
 
RsrII CG'GwC_CG

Cuts at:   3894    3894 
   Size:       5699
 
SacI G_AGCT'C

Cuts at:    127     445    2713     127 
   Size:        318    2268    3113

  Fragments arranged by size: 

                3113    2268     318
 
SalI G'TCGA_C

Cuts at:   2736    2736 
   Size:       5699
 
SapI GCTCTTCn'nnn_

Cuts at:   3722    3932    3722 
   Size:        210    5489
 
SbfI CC_TGCA'GG

Cuts at:   2746    2746 
   Size:       5699
 
ScaI AGT'ACT

Cuts at:   5216    5216 
   Size:       5699
 
SfcI C'TryA_G

Cuts at:     33    1014    1650    2242    2742    3427    4420    4611      33 
   Size:        981     636     592     500     685     993     191    1121

  Fragments arranged by size: 

                1121     993     981     685     636     592     500     191
            
 
SfoI GGC'GCC

Cuts at:   3131    3379    3131 
   Size:        248    5451
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       5699
 
SmaI CCC'GGG

Cuts at:   2721    2721 
   Size:       5699
 
SmlI C'TyrA_G

Cuts at:    126     280    2854    3769    4261    4523     126 
   Size:        154    2574     915     492     262    1302

  Fragments arranged by size: 

                2574    1302     915     492     262     154
 
SpeI A'CTAG_T

Cuts at:     67    2422      67 
   Size:       2355    3344
 
SphI G_CATG'C

Cuts at:   1273    2212    2752    3252    1273 
   Size:        939     540     500    3720

  Fragments arranged by size: 

                3720     939     540     500
 
SspI AAT'ATT

Cuts at:    267     636    2342     267 
   Size:        369    1706    3624

  Fragments arranged by size: 

                3624    1706     369
 
StuI AGG'CCT

Cuts at:   1375    1375 
   Size:       5699
 
StyI C'CwwG_G

Cuts at:    107    1292    2640    2833    3187     107 
   Size:       1185    1348     193     354    2619

  Fragments arranged by size: 

                2619    1348    1185     354     193
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   2617    2665    3282    3415    3874    4083    2617 
   Size:         48     617     133     459     209    4233

  Fragments arranged by size: 

                4233     617     459     209     133      48
 
TatI w'GTAC_w

Cuts at:    130    5214     130 
   Size:       5084     615
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   5216    5248    5216 
   Size:         32    5667
 
Tth111I GACn'n_nGTC

Cuts at:   3496    3496 
   Size:       5699
 
XbaI T'CTAG_A

Cuts at:   2730    2730 
   Size:       5699
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   3194    3194 
   Size:       5699
 
XhoI C'TCGA_G

Cuts at:    126     126 
   Size:       5699
 
XmaI C'CCGG_G

Cuts at:   2719    2719 
   Size:       5699
 
XmnI GAAnn'nnTTC

Cuts at:    954    1856    2318     954 
   Size:        902     462    4335

  Fragments arranged by size: 

                4335     902     462
 
ZraI GAC'GTC

Cuts at:   2454    2454 
   Size:       5699
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BssSI   BstAPI
    BstBI    BstYI     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FspI    HaeII    Hin4I
   HincII  HindIII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NsiI     NspI     PasI
     PciI     PfoI     PmeI     PpiI    PshAI     PsiI    PspXI     PstI
     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI     ScaI
     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AfeI     AhdI     AloI     ApaI     AscI    AvrII     BglI     BmtI
    BsaXI    BseRI    BsiWI    BsmBI    BsrGI   BssHII   BstEII    BstXI
  BstZ17I   Bsu36I     FseI    FspAI     HpaI     MfeI     NdeI     NheI
     NotI     NruI     PacI    PflMI     PmlI    PpuMI   PspOMI     PsrI
    SacII    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SwaI
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