Restriction map for pF1KB8500
 (Linear) (Six-base) MAPSORT of: KIBB8500.seq  Check: 605  from: 1  to: 2586



KIBB8500.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:28  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      78    2586 
   Size:         78    2508
 
AatII G_ACGT'C

Cuts at:      0    2348    2586 
   Size:       2348     238
 
AccI GT'mk_AC

Cuts at:      0     926    2586 
   Size:        926    1660
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1358    2586 
   Size:       1358    1228
 
AflII C'TTAA_G

Cuts at:      0     172    2586 
   Size:        172    2414
 
AflIII A'CryG_T

Cuts at:      0     932    1241    1593    1796    2586 
   Size:        932     309     352     203     790

  Fragments arranged by size: 

                 932     790     352     309     203
 
AleI CACnn'nnGTG

Cuts at:      0    1498    2586 
   Size:       1498    1088
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     921    2435    2586 
   Size:        921    1514     151

  Fragments arranged by size: 

                1514     921     151
 
AlwNI CAG_nnn'CTG

Cuts at:      0      31    1128    1541    2284    2432    2586 
   Size:         31    1097     413     743     148     154

  Fragments arranged by size: 

                1097     743     413     154     148      31
 
ApaLI G'TGCA_C

Cuts at:      0     344    2586 
   Size:        344    2242
 
ApoI r'AATT_y

Cuts at:      0     514    1025    1681    1748    2586 
   Size:        514     511     656      67     838

  Fragments arranged by size: 

                 838     656     514     511      67
 
AseI AT'TA_AT

Cuts at:      0    1832    2586 
   Size:       1832     754
 
AvaI C'yCGr_G

Cuts at:      0      18    1479    2402    2586 
   Size:         18    1461     923     184

  Fragments arranged by size: 

                1461     923     184      18
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0     934     967    2586 
   Size:        934      33    1619

  Fragments arranged by size: 

                1619     934      33
 
BanI G'GyrC_C

Cuts at:      0     148    1378    2586 
   Size:        148    1230    1208

  Fragments arranged by size: 

                1230    1208     148
 
BanII G_rGCy'C

Cuts at:      0      19     337    1336    1585    2586 
   Size:         19     318     999     249    1001

  Fragments arranged by size: 

                1001     999     318     249      19
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1355    2586 
   Size:       1355    1231
 
BbvCI CC'TCA_GC

Cuts at:      0    1697    2586 
   Size:       1697     889
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    2254    2288    2586 
   Size:       2254      34     298

  Fragments arranged by size: 

                2254     298      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     962    2586 
   Size:        962    1624
 
BclI T'GATC_A

Cuts at:      0     411    2489    2586 
   Size:        411    2078      97

  Fragments arranged by size: 

                2078     411      97
 
BfrBI ATG'CAT

Cuts at:      0     568    1161    2586 
   Size:        568     593    1425

  Fragments arranged by size: 

                1425     593     568
 
BglII A'GATC_T

Cuts at:      0    1405    2586 
   Size:       1405    1181
 
BlpI GC'TnA_GC

Cuts at:      0    2368    2586 
   Size:       2368     218
 
Bme1580I G_kGCm'C

Cuts at:      0     348    1487    1850    2586 
   Size:        348    1139     363     736

  Fragments arranged by size: 

                1139     736     363     348
 
BmgBI CAC'GTC

Cuts at:      0    1242    2586 
   Size:       1242    1344
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1436    2586 
   Size:       1436    1150
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1494    2586 
   Size:       1494    1092
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     995    1244    1301    1982    2137    2261    2409    2586 
   Size:        995     249      57     681     155     124     148     177

  Fragments arranged by size: 

                 995     681     249     177     155     148     124      57
            
 
Bpu10I CC'TnA_GC

Cuts at:      0      35    1697    2586 
   Size:         35    1662     889

  Fragments arranged by size: 

                1662     889      35
 
BsaHI Gr'CG_yC

Cuts at:      0    1397    2345    2586 
   Size:       1397     948     241

  Fragments arranged by size: 

                1397     948     241
 
BsaWI w'CCGG_w

Cuts at:      0    1672    2586 
   Size:       1672     914
 
BseYI C'CCAG_C

Cuts at:      0     661     894    2586 
   Size:        661     233    1692

  Fragments arranged by size: 

                1692     661     233
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     496    2586 
   Size:        496    2090
 
BsiHKAI G_wGCw'C

Cuts at:      0      19     337     348    2153    2586 
   Size:         19     318      11    1805     433

  Fragments arranged by size: 

                1805     433     318      19      11
 
BsmI GAATG_Cn'

Cuts at:      0     994    2586 
   Size:        994    1592
 
Bsp1286I G_dGCh'C

Cuts at:      0      19     337     348    1336    1487    1585    1850    2153 
   Size:         19     318      11     988     151      98     265     303

Cuts at:   2153    2586 
   Size:        433

  Fragments arranged by size: 

                 988     433     318     303     265     151      98      19
                  11
 
BspHI T'CATG_A

Cuts at:      0    1899    2586 
   Size:       1899     687
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      39      78     379    2586 
   Size:         39      39     301    2207

  Fragments arranged by size: 

                2207     301      39      39
 
BsrBI CCG'CTC

Cuts at:      0    1462    2586 
   Size:       1462    1124
 
BsrFI r'CCGG_y

Cuts at:      0     878    2393    2586 
   Size:        878    1515     193

  Fragments arranged by size: 

                1515     878     193
 
BssSI C'ACGA_G

Cuts at:      0     913    2586 
   Size:        913    1673
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0    1029    1128    1541    2148    2586 
   Size:       1029      99     413     607     438

  Fragments arranged by size: 

                1029     607     438     413      99
 
BstBI TT'CG_AA

Cuts at:      0     239    2586 
   Size:        239    2347
 
BstYI r'GATC_y

Cuts at:      0     189    1405    2586 
   Size:        189    1216    1181

  Fragments arranged by size: 

                1216    1181     189
 
BtgI C'CryG_G

Cuts at:      0    1184    1499    2105    2532    2586 
   Size:       1184     315     606     427      54

  Fragments arranged by size: 

                1184     606     427     315      54
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     368    2541    2586 
   Size:        368    2173      45

  Fragments arranged by size: 

                2173     368      45
 
BtsI GCAGTG_nn'

Cuts at:      0     107    1126    1427    1539    2586 
   Size:        107    1019     301     112    1047

  Fragments arranged by size: 

                1047    1019     301     112     107
 
ClaI AT'CG_AT

Cuts at:      0    2264    2586 
   Size:       2264     322
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     141     176     198     233    2586 
   Size:        141      35      22      35    2353

  Fragments arranged by size: 

                2353     141      35      35      22
 
DraI TTT'AAA

Cuts at:      0    1064    1737    2586 
   Size:       1064     673     849

  Fragments arranged by size: 

                1064     849     673
 
DraIII CAC_nnn'GTG

Cuts at:      0     343    2148    2586 
   Size:        343    1805     438

  Fragments arranged by size: 

                1805     438     343
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    1396    2586 
   Size:       1396    1190
 
EaeI y'GGCC_r

Cuts at:      0    1559    2586 
   Size:       1559    1027
 
EarI CTCTTCn'nnn_

Cuts at:      0     752    1280    2586 
   Size:        752     528    1306

  Fragments arranged by size: 

                1306     752     528
 
EcoICRI GAG'CTC

Cuts at:      0      17     335    2586 
   Size:         17     318    2251

  Fragments arranged by size: 

                2251     318      17
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     995    1244    1301    1358    1982    2137    2261    2409 
   Size:        995     249      57      57     624     155     124     148

Cuts at:   2409    2586 
   Size:        177

  Fragments arranged by size: 

                 995     624     249     177     155     148     124      57
                  57
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     295    2586 
   Size:        295    2291
 
EcoRV GAT'ATC

Cuts at:      0     999    2082    2586 
   Size:        999    1083     504

  Fragments arranged by size: 

                1083     999     504
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1768    2139    2265    2586 
   Size:       1768     371     126     321

  Fragments arranged by size: 

                1768     371     321     126
 
HaeII r_GCGC'y

Cuts at:      0    1618    2586 
   Size:       1618     968
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      46      78     396     428    1416    1448    1567    1599 
   Size:         46      32     318      32     988      32     119      32

Cuts at:   1599    2208    2240    2586 
   Size:        609      32     346

  Fragments arranged by size: 

                 988     609     346     318     119      46      32      32
                  32      32      32
 
HincII GTy'rAC

Cuts at:      0    1101    2586 
   Size:       1101    1485
 
HindIII A'AGCT_T

Cuts at:      0     169    1045    2586 
   Size:        169     876    1541

  Fragments arranged by size: 

                1541     876     169
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     372     819    2586 
   Size:        372     447    1767

  Fragments arranged by size: 

                1767     447     372
 
MslI CAynn'nnrTG

Cuts at:      0    1498    2497    2586 
   Size:       1498     999      89

  Fragments arranged by size: 

                1498     999      89
 
MspA1I CmG'CkG

Cuts at:      0     732    1168    2586 
   Size:        732     436    1418

  Fragments arranged by size: 

                1418     732     436
 
NaeI GCC'GGC

Cuts at:      0    2395    2586 
   Size:       2395     191
 
NcoI C'CATG_G

Cuts at:      0    1184    2532    2586 
   Size:       1184    1348      54

  Fragments arranged by size: 

                1348    1184      54
 
NgoMIV G'CCGG_C

Cuts at:      0    2393    2586 
   Size:       2393     193
 
NsiI A_TGCA'T

Cuts at:      0     570    1163    2586 
   Size:        570     593    1423

  Fragments arranged by size: 

                1423     593     570
 
NspI r_CATG'y

Cuts at:      0     568     936    1165    1597    1800    2104    2586 
   Size:        568     368     229     432     203     304     482

  Fragments arranged by size: 

                 568     482     432     368     304     229     203
 
PasI CC'CwG_GG

Cuts at:      0    1570    2380    2586 
   Size:       1570     810     206

  Fragments arranged by size: 

                1570     810     206
 
PciI A'CATG_T

Cuts at:      0     932    1593    1796    2586 
   Size:        932     661     203     790

  Fragments arranged by size: 

                 932     790     661     203
 
PfoI T'CCnGG_A

Cuts at:      0    1959    2586 
   Size:       1959     627
 
PshAI GACnn'nnGTC

Cuts at:      0     466    1769    2586 
   Size:        466    1303     817

  Fragments arranged by size: 

                1303     817     466
 
PsiI TTA'TAA

Cuts at:      0    2059    2586 
   Size:       2059     527
 
PspXI vC'TCGA_Gb

Cuts at:      0      18    2586 
   Size:         18    2568
 
PstI C_TGCA'G

Cuts at:      0     910    1546    2138    2586 
   Size:        910     636     592     448

  Fragments arranged by size: 

                 910     636     592     448
 
PvuII CAG'CTG

Cuts at:      0     732    2586 
   Size:        732    1854
 
SacI G_AGCT'C

Cuts at:      0      19     337    2586 
   Size:         19     318    2249

  Fragments arranged by size: 

                2249     318      19
 
SfcI C'TryA_G

Cuts at:      0     906    1542    2134    2586 
   Size:        906     636     592     452

  Fragments arranged by size: 

                 906     636     592     452
 
SmlI C'TyrA_G

Cuts at:      0      18     172    2586 
   Size:         18     154    2414

  Fragments arranged by size: 

                2414     154      18
 
SpeI A'CTAG_T

Cuts at:      0    2314    2586 
   Size:       2314     272
 
SphI G_CATG'C

Cuts at:      0    1165    2104    2586 
   Size:       1165     939     482

  Fragments arranged by size: 

                1165     939     482
 
SspI AAT'ATT

Cuts at:      0     159     528    2234    2586 
   Size:        159     369    1706     352

  Fragments arranged by size: 

                1706     369     352     159
 
StuI AGG'CCT

Cuts at:      0    1267    2586 
   Size:       1267    1319
 
StyI C'CwwG_G

Cuts at:      0    1184    2532    2586 
   Size:       1184    1348      54

  Fragments arranged by size: 

                1348    1184      54
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    2509    2557    2586 
   Size:       2509      48      29

  Fragments arranged by size: 

                2509      48      29
 
TatI w'GTAC_w

Cuts at:      0      22    2586 
   Size:         22    2564
 
XhoI C'TCGA_G

Cuts at:      0      18    2586 
   Size:         18    2568
 
XmnI GAAnn'nnTTC

Cuts at:      0     846    1748    2210    2586 
   Size:        846     902     462     376

  Fragments arranged by size: 

                 902     846     462     376
 
ZraI GAC'GTC

Cuts at:      0    2346    2586 
   Size:       2346     240
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI    AflII   AflIII     AleI     AlfI
    AlwNI    ApaLI     ApoI     AseI     AvaI     BaeI     BanI    BanII
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BplI     BpmI   Bpu10I    BsaHI    BsaWI
    BseYI     BsgI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI    BsrBI
    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EarI  EcoICRI
  Eco57MI    EcoNI    EcoRV     FalI    HaeII    Hin4I   HincII  HindIII
     MmeI     MslI   MspA1I     NaeI     NcoI   NgoMIV     NsiI     NspI
     PasI     PciI     PfoI    PshAI     PsiI    PspXI     PstI    PvuII
     SacI     SfcI     SmlI     SpeI     SphI     SspI     StuI     StyI
    TaqII     TatI     XhoI     XmnI     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI     AgeI     AhdI     AloI     ApaI     AscI
    AsiSI    AvrII    BamHI     BbeI     BglI     BmtI    BpuEI     BsaI
    BsaAI    BsaBI    BsaXI    BseRI    BsiEI    BsiWI    BsmBI    BspEI
    BsrDI    BsrGI   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     EagI
     EciI EcoO109I    EcoRI     FseI     FspI    FspAI     HpaI     KasI
     KpnI     MfeI     MluI     MscI     NarI     NdeI     NheI     NotI
     NruI     PacI    PflMI     PmeI     PmlI     PpiI    PpuMI   PspOMI
     PsrI     PvuI    RsrII    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SrfI     SwaI     TstI  Tth111I     XbaI     XcmI     XmaI
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