Restriction map for pF1KB9948
##################################
# NO CUT ENZYMES (   99 enzymes) #
##################################

Acc65I      AccI        AccIII      AclI        AflII       
AgeI        AhdI        ApaI        AscI        AseI        
AsnI        Asp718I     AspEI       AspI        AviII       
AvrII       BamHI       BbrPI       BbvCI       BciVI       
BclI        BfaI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BsaBI       BseAI       BsiWI       
BsmBI       BsmI        Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrGI       
BssHII      BssSI       Bst1107I    BstEII      BstPI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraIII      Eam1105I    EcoO65I     FbaI        FseI        
FspI        HgaI        KpnI        MaeI        MamI        
MfeI        MluI        MroI        MunI        NaeI        
NdeI        NgoMIV      NheI        NruI        PacI        
PaeR7I      PflFI       PinAI       PmaCI       PmeI        
PmlI        PshBI       Psp1406I    PvuI        RcaI        
RsrII       SalI        SapI        SbfI        SexAI       
SfiI        SgrAI       SmaI        SmlI        SpeI        
Sse8387I    SspBI       StuI        SwaI        Tth111I     
XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   62 enzymes) #
###################################

AatII         511  GACGTC               GACGT^C
AccII         475  CGCG                 CG^CG
AflIII        320  ACACGT               A^C[GA][CT]GT
AlwNI         150  CAGCCACTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI           75  CCTCAGG              CC^T[ACGT]AGG
Aor51HI       422  AGCGCT               AGC^GCT
Asp700I       756  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI         1528  CCCGAG               C^[CT]CG[GA]G
BalI         1706  TGGCCA               TGG^CCA
BbeI           70  GGCGCC               GGCGC^C
BcgI         1530  CGAGATAATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI          446  GCCTGTGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaI         1001  GAGACC               GGTCTC(1/5)
BseRI          40  GAGGAG               GAGGAG(10/8)
BsgI          156  CTGCAC               GTGCAG(16/14)
BsoBI        1528  CCCGAG               C^[CT]CG[GA]G
BspMI         400  ACCTGC               ACCTGC(4/8)
BsrFI        1307  GCCGGT               [GA]^CCGG[CT]
BstBI        1966  TTCGAA               TT^CGAA
BstUI         475  CGCG                 CG^CG
Bsu36I         75  CCTCAGG              CC^T[ACGT]AGG
Cfr10I       1307  GCCGGT               [GA]^CCGG[CT]
DraI         1998  TTTAAA               TTT^AAA
DraII         944  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EarI         1645  CTCTTC               CTCTTC(1/4)
Eco47III      422  AGCGCT               AGC^GCT
Eco81I         75  CCTCAGG              CC^T[ACGT]AGG
EcoNI        1116  CCTGAAGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I      944  AGGTCCC              [GA]G^G[ACGT]CC[CT]
EcoRI        1963  GAATTC               G^AATTC
EcoRV        1777  GATATC               GAT^ATC
EcoT22I      1790  ATGCAT               ATGCA^T
HincII       1984  GTTAAC               GT[CT]^[GA]AC
HindII       1984  GTTAAC               GT[CT]^[GA]AC
HindIII      1859  AAGCTT               A^AGCTT
HpaI         1984  GTTAAC               GTT^AAC
KasI           70  GGCGCC               G^GCGCC
Ksp632I      1645  CTCTTC               CTCTTC(1/4)
KspI          474  CCGCGG               CCGC^GG
MluNI        1706  TGGCCA               TGG^CCA
MscI         1706  TGGCCA               TGG^CCA
MvnI          475  CGCG                 CG^CG
NarI           70  GGCGCC               GG^CGCC
NcoI         1235  CCATGG               C^CATGG
NotI          476  GCGGCCGC             GC^GGCCGC
NsiI         1790  ATGCAT               ATGCA^T
NspV         1966  TTCGAA               TT^CGAA
PflMI        1230  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI         944  AGGTCCC              [GA]G^G[AT]CC[CT]
PshAI         268  GACAAGGGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         688  CAGCTG               CAG^CTG
SacI         1096  GAGCTC               GAGCT^C
SacII         474  CCGCGG               CCGC^GG
ScaI          851  AGTACT               AGT^ACT
SfoI           70  GGCGCC               GGC^GCC
SfuI         1966  TTCGAA               TT^CGAA
SnaBI         583  TACGTA               TAC^GTA
SphI         1788  GCATGC               GCATG^C
SspI         1948  AATATT               AAT^ATT
Van91I       1230  CCAGACCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI         1694  CCAAGGAAGCTGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          756  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AcyI           70  GGCGCC               G[GA]^CG[CT]C
              511  GACGTC              
BanII        1096  GAGCTC               G[GA]GC[CT]^C
             1318  GAGCCC              
BbsI          630  GAAGAC               GAAGAC(2/6)
              713  GAAGAC              
BcnI            9  CCCGG                CC^[GC]GG
              948  CCCGG               
BpmI          327  CTCCAG               CTGGAG(16/14)
             1511  CTCCAG              
BpuAI         630  GAAGAC               GAAGAC(2/6)
              713  GAAGAC              
BsaHI          70  GGCGCC               G[GA]^CG[CT]C
              511  GACGTC              
BsaWI        1004  ACCGGA               [AT]^CCGG[AT]
             1799  TCCGGT              
BstAPI        149  GCAGCCACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1342  GCAGAAATTGC         
DrdI          505  GACAGGGACGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1930  GACCAGGAAGTC        
EagI          477  CGGCCG               C^GGCCG
             1305  CGGCCG              
EclXI         477  CGGCCG               C^GGCCG
             1305  CGGCCG              
Eco52I        477  CGGCCG               C^GGCCG
             1305  CGGCCG              
Hin1I          70  GGCGCC               G[GA]^CG[CT]C
              511  GACGTC              
MslI          399  CACCTGCATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             1158  CACTATGGTG          
MspA1I        474  CCGCGG               C[AC]G^C[GT]G
              688  CAGCTG              
NciI            9  CCCGG                CC^[GC]GG
              948  CCCGG               
NspI         1457  GCATGT               [GA]CATG^[CT]
             1788  GCATGC              
PleI          371  GACTC                GAGTC(4/5)
             1583  GAGTC               
PstI          147  CTGCAG               CTGCA^G
             1340  CTGCAG              
SfcI          147  CTGCAG               C^T[GA][CT]AG
             1340  CTGCAG              


#########################################
# 3 OR MORE CUT ENZYMES (   76 enzymes) #
#########################################

AciI        AcsI        AfaI        AluI        Alw44I      
AlwI        ApaLI       ApoI        AspHI       AvaII       
BanI        BbvI        BmyI        BsaAI       BsaJI       
BsiEI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       BstYI       Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DsaI        EaeI        EcoRII      
EcoT14I     FauI        Fnu4HI      FokI        HaeII       
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspI        MvaI        MwoI        NdeII       NlaIII      
NlaIV       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       StyI        TaqI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       TthHB8I     
XhoII       
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