Restriction map for pF1KB4832
##################################
# NO CUT ENZYMES (  130 enzymes) #
##################################

Acc65I      AccIII      AclI        AflII       AflIII      
AgeI        AhdI        Alw44I      AocI        Aor51HI     
ApaI        ApaLI       AscI        Asp718I     AspEI       
AvaII       AviII       AvrII       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BclI        BfrI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaI        BsaWI       BseAI       BsgI        BsiWI       
BsmFI       Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstXI       BstZ17I     
Bsu36I      CelII       ClaI        CpoI        DraI        
DraIII      DrdI        DsaI        Eam1105I    EarI        
Eco47III    Eco81I      EcoRI       EcoRV       EcoT22I     
FbaI        FseI        FspI        HaeII       HindIII     
HinfI       HpaI        KasI        KpnI        Ksp632I     
KspI        MamI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SapI        SbfI        
ScaI        SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SwaI        TfiI        TspRI       
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AccI          172  GTCGAC               GT^[AC][GT]AC
AccII         381  CGCG                 CG^CG
AcsI          598  AAATTC               [GA]^AATT[CT]
ApoI          598  AAATTC               [GA]^AATT[CT]
AseI          769  ATTAAT               AT^TAAT
AsnI          769  ATTAAT               AT^TAAT
Asp700I       364  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI          268  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI         1186  CCCGAG               C^[CT]CG[GA]G
BalI          473  TGGCCA               TGG^CCA
BamHI         950  GGATCC               G^GATCC
BfaI         1069  CTAG                 C^TAG
BglI          659  GCCACTTTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI         447  CGGCCG               CG[GA][CT]^CG
BsmBI         905  GAGACG               CGTCTC(1/5)
BsmI          368  GCATTC               GAATGC(1/-1)
BsoBI        1186  CCCGAG               C^[CT]CG[GA]G
BsrDI         617  GCAATG               GCAATG(2/0)
BsrI          699  ACTGG                ACTGG(1/-1)
BstAPI       1167  GCAGGAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI         381  CGCG                 CG^CG
CfoI          380  GCGC                 GCG^C
DraII        1064  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EagI          447  CGGCCG               C^GGCCG
EclXI         447  CGGCCG               C^GGCCG
Eco52I        447  CGGCCG               C^GGCCG
EcoNI         543  CCTGAAGGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I     1064  GGGGCCT              [GA]G^G[ACGT]CC[CT]
EcoT14I       389  CCAAGG               C^C[AT][AT]GG
FauI           10  GCGGG                CCCGC(4/6)
HgaI          907  GACGC                GACGC(5/10)
HhaI          380  GCGC                 GCG^C
HinP1I        380  GCGC                 G^CGC
HincII        172  GTCGAC               GT[CT]^[GA]AC
HindII        172  GTCGAC               GT[CT]^[GA]AC
MaeI         1069  CTAG                 C^TAG
MluNI         473  TGGCCA               TGG^CCA
MscI          473  TGGCCA               TGG^CCA
MseI          770  TTAA                 T^TAA
MslI          930  CACCCTGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI          381  CGCG                 CG^CG
NlaIII        656  CATG                 CATG^
PflFI         268  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshBI         769  ATTAAT               AT^TAAT
PvuII         425  CAGCTG               CAG^CTG
SalI          172  GTCGAC               G^TCGAC
SspI          772  AATATT               AAT^ATT
StuI          110  AGGCCT               AGG^CCT
StyI          389  CCAAGG               C^C[AT][AT]GG
Tru9I         770  TTAA                 T^TAA
Tth111I       268  GACTACGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          689  CCATCTTCCGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          364  GAAGGCATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   20 enzymes) #
###################################

AatII         169  GACGTC               GACGT^C
              628  GACGTC              
AcyI          169  GACGTC               G[GA]^CG[CT]C
              628  GACGTC              
AspHI         384  GTGCTC               G[AT]GC[AT]^C
             1260  GAGCAC              
BanI          481  GGCACC               G^G[CT][GA]CC
              503  GGTGCC              
BglII         491  AGATCT               A^GATCT
              640  AGATCT              
BsaHI         169  GACGTC               G[GA]^CG[CT]C
              628  GACGTC              
BseRI         867  CTCCTC               GAGGAG(10/8)
              532  GAGGAG              
BsiHKAI       384  GTGCTC               G[AT]GC[AT]^C
             1260  GAGCAC              
BsrFI          98  ACCGGC               [GA]^CCGG[CT]
              445  ACCGGC              
BstEII        160  GGTTACC              G^GT[ACGT]ACC
              309  GGTCACC             
BstPI         160  GGTTACC              G^GT[ACGT]ACC
              309  GGTCACC             
Cfr10I         98  ACCGGC               [GA]^CCGG[CT]
              445  ACCGGC              
EaeI          447  CGGCCG               [CT]^GGCC[GA]
              473  TGGCCA              
EcoO65I       160  GGTTACC              G^GT[ACGT]ACC
              309  GGTCACC             
Hin1I         169  GACGTC               G[GA]^CG[CT]C
              628  GACGTC              
SexAI         128  ACCTGGT              A^CC[AT]GGT
              998  ACCTGGT             
SfaNI        1155  GATGC                GCATC(5/9)
             1102  GCATC               
TaqI           41  TCGA                 T^CGA
              173  TCGA                
Tsp509I       599  AATT                 ^AATT
              768  AATT                
TthHB8I        41  TCGA                 T^CGA
              173  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   55 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
BanII       BbsI        BbvI        BcnI        BmyI        
BpmI        BpuAI       BsaJI       BsiYI       BslI        
BsmAI       Bsp1286I    BssKI       BstF5I      BstNI       
BstYI       Cac8I       Cfr13I      DdeI        DpnII       
EcoRII      Fnu4HI      FokI        HaeIII      HapII       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIV       PstI        RsaI        Sau3AI      Sau96I      
ScrFI       SfcI        TseI        Tsp45I      XhoII       
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