Restriction map for pF1KB4203
 (Linear) (Six-base) MAPSORT of: KIBB4203.seq  Check: 8684  from: 1  to: 954



KIBB4203.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:03  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     741     954 
   Size:        741     213
 
AatII G_ACGT'C

Cuts at:      0     665     954 
   Size:        665     289
 
AccI GT'mk_AC

Cuts at:      0     266     954 
   Size:        266     688
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     766     954 
   Size:        766     188
 
AflIII A'CryG_T

Cuts at:      0     317     954 
   Size:        317     637
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      31      63     954 
   Size:         31      32     891

  Fragments arranged by size: 

                 891      32      31
 
AlwNI CAG_nnn'CTG

Cuts at:      0     744     828     954 
   Size:        744      84     126

  Fragments arranged by size: 

                 744     126      84
 
ApaI G_GGCC'C

Cuts at:      0     441     954 
   Size:        441     513
 
AvaI C'yCGr_G

Cuts at:      0     799     954 
   Size:        799     155
 
BamHI G'GATC_C

Cuts at:      0     194     954 
   Size:        194     760
 
BanI G'GyrC_C

Cuts at:      0     172     954 
   Size:        172     782
 
BanII G_rGCy'C

Cuts at:      0     338     441     954 
   Size:        338     103     513

  Fragments arranged by size: 

                 513     338     103
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     913     953     954 
   Size:        913      40       1

  Fragments arranged by size: 

                 913      40       1
 
Bme1580I G_kGCm'C

Cuts at:      0     300     441     954 
   Size:        300     141     513

  Fragments arranged by size: 

                 513     300     141
 
BmgBI CAC'GTC

Cuts at:      0     318     954 
   Size:        318     636
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     130     954 
   Size:        130     824
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     687     883     954 
   Size:        463     224     196      71

  Fragments arranged by size: 

                 463     224     196      71
 
BsaBI GATnn'nnATC

Cuts at:      0     697     954 
   Size:        697     257
 
BsaHI Gr'CG_yC

Cuts at:      0     662     954 
   Size:        662     292
 
BsaWI w'CCGG_w

Cuts at:      0     365     954 
   Size:        365     589
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      31      61     751     778     781     808     954 
   Size:         31      30     690      27       3      27     146

  Fragments arranged by size: 

                 690     146      31      30      27      27       3
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     775     954 
   Size:        775     179
 
BseYI C'CCAG_C

Cuts at:      0     773     780     954 
   Size:        773       7     174

  Fragments arranged by size: 

                 773     174       7
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     954 
   Size:        436     518
 
BsiEI CG_ry'CG

Cuts at:      0     266     789     954 
   Size:        266     523     165

  Fragments arranged by size: 

                 523     266     165
 
BsiHKAI G_wGCw'C

Cuts at:      0     338     878     954 
   Size:        338     540      76

  Fragments arranged by size: 

                 540     338      76
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     308     954 
   Size:        308     646
 
Bsp1286I G_dGCh'C

Cuts at:      0     300     338     441     878     954 
   Size:        300      38     103     437      76

  Fragments arranged by size: 

                 437     300     103      76      38
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     741     954 
   Size:        741     213
 
BsrBI CCG'CTC

Cuts at:      0     170     954 
   Size:        170     784
 
BsrDI GCAATG_nn'

Cuts at:      0     914     954 
   Size:        914      40
 
BsrFI r'CCGG_y

Cuts at:      0     586     954 
   Size:        586     368
 
BstEII G'GTnAC_C

Cuts at:      0     105     954 
   Size:        105     849
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     579     954 
   Size:        579     375
 
BstYI r'GATC_y

Cuts at:      0     194     323     935     954 
   Size:        194     129     612      19

  Fragments arranged by size: 

                 612     194     129      19
 
Bsu36I CC'TnA_GG

Cuts at:      0     829     954 
   Size:        829     125
 
BtgI C'CryG_G

Cuts at:      0     126     954 
   Size:        126     828
 
BtsI GCAGTG_nn'

Cuts at:      0     815     954 
   Size:        815     139
 
EaeI y'GGCC_r

Cuts at:      0     182     569     695     954 
   Size:        182     387     126     259

  Fragments arranged by size: 

                 387     259     182     126
 
EcoICRI GAG'CTC

Cuts at:      0     336     954 
   Size:        336     618
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     463     687     766     883     954 
   Size:        463     224      79     117      71

  Fragments arranged by size: 

                 463     224     117      79      71
 
EcoO109I rG'GnC_Cy

Cuts at:      0     438     728     954 
   Size:        438     290     226

  Fragments arranged by size: 

                 438     290     226
 
FspI TGC'GCA

Cuts at:      0     259     954 
   Size:        259     695
 
FspAI rTGC'GCAy

Cuts at:      0     259     954 
   Size:        259     695
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     751     778     783     810     954 
   Size:        751      27       5      27     144

  Fragments arranged by size: 

                 751     144      27      27       5
 
HincII GTy'rAC

Cuts at:      0     267     954 
   Size:        267     687
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     683     954 
   Size:        683     271
 
MscI TGG'CCA

Cuts at:      0     571     697     954 
   Size:        571     126     257

  Fragments arranged by size: 

                 571     257     126
 
MslI CAynn'nnrTG

Cuts at:      0     178     184     253     479     682     954 
   Size:        178       6      69     226     203     272

  Fragments arranged by size: 

                 272     226     203     178      69       6
 
MspA1I CmG'CkG

Cuts at:      0     744     954 
   Size:        744     210
 
NaeI GCC'GGC

Cuts at:      0     588     954 
   Size:        588     366
 
NgoMIV G'CCGG_C

Cuts at:      0     586     954 
   Size:        586     368
 
NspI r_CATG'y

Cuts at:      0     258     954 
   Size:        258     696
 
PfoI T'CCnGG_A

Cuts at:      0      49     954 
   Size:         49     905
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      31      63     954 
   Size:         31      32     891

  Fragments arranged by size: 

                 891      32      31
 
PpuMI rG'GwC_Cy

Cuts at:      0     728     954 
   Size:        728     226
 
PspOMI G'GGCC_C

Cuts at:      0     437     954 
   Size:        437     517
 
PvuII CAG'CTG

Cuts at:      0     744     954 
   Size:        744     210
 
SacI G_AGCT'C

Cuts at:      0     338     954 
   Size:        338     616
 
SalI G'TCGA_C

Cuts at:      0     265     954 
   Size:        265     689
 
SanDI GG'GwC_CC

Cuts at:      0     728     954 
   Size:        728     226
 
StyI C'CwwG_G

Cuts at:      0     350     408     954 
   Size:        350      58     546

  Fragments arranged by size: 

                 546     350      58
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      33     954 
   Size:         33     921
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     117     690     954 
   Size:        117     573     264

  Fragments arranged by size: 

                 573     264     117
 
ZraI GAC'GTC

Cuts at:      0     663     954 
   Size:        663     291
 

 Enzymes that do cut:

     AarI    AatII     AccI     AcuI   AflIII     AloI    AlwNI     ApaI
     AvaI    BamHI     BanI    BanII     BbsI Bme1580I    BmgBI     BmrI
     BpmI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BsrFI
   BstEII    BstXI    BstYI   Bsu36I     BtgI     BtsI     EaeI  EcoICRI
  Eco57MI EcoO109I     FspI    FspAI    Hin4I   HincII     MmeI     MscI
     MslI   MspA1I     NaeI   NgoMIV     NspI     PfoI     PpiI    PpuMI
   PspOMI    PvuII     SacI     SalI    SanDI     StyI    TaqII     XcmI
     ZraI

 Enzymes that do not cut: 

   Acc65I     AclI     AfeI    AflII     AgeI     AhdI     AleI     AlfI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsiWI     BsmI
    BspEI    BspHI    BsrGI   BssHII    BssSI   BstAPI    BstBI  BstZ17I
    BtgZI     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI
     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI    HaeII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     NarI     NcoI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI
     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PstI     PvuI
    RsrII    SacII     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI
     XhoI     XmaI     XmnI
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