Restriction map for pF1KB3434
 (Linear) (Six-base) MAPSORT of: KIBB3434.seq  Check: 5379  from: 1  to: 969



KIBB3434.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

Acc65I G'GTAC_C

Cuts at:      0     674     969 
   Size:        674     295
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     553     969 
   Size:        163     390     416

  Fragments arranged by size: 

                 416     390     163
 
AflIII A'CryG_T

Cuts at:      0     389     969 
   Size:        389     580
 
AleI CACnn'nnGTG

Cuts at:      0     403     969 
   Size:        403     566
 
AlwNI CAG_nnn'CTG

Cuts at:      0     426     969 
   Size:        426     543
 
ApaLI G'TGCA_C

Cuts at:      0     609     724     969 
   Size:        609     115     245

  Fragments arranged by size: 

                 609     245     115
 
AscI GG'CGCG_CC

Cuts at:      0     735     969 
   Size:        735     234
 
AvaI C'yCGr_G

Cuts at:      0      65     808     877     969 
   Size:         65     743      69      92

  Fragments arranged by size: 

                 743      92      69      65
 
AvrII C'CTAG_G

Cuts at:      0     162     969 
   Size:        162     807
 
BanI G'GyrC_C

Cuts at:      0     103     674     817     969 
   Size:        103     571     143     152

  Fragments arranged by size: 

                 571     152     143     103
 
BanII G_rGCy'C

Cuts at:      0      66     815     969 
   Size:         66     749     154

  Fragments arranged by size: 

                 749     154      66
 
BbeI G_GCGC'C

Cuts at:      0     821     969 
   Size:        821     148
 
BglI GCCn_nnn'nGGC

Cuts at:      0     347     714     969 
   Size:        347     367     255

  Fragments arranged by size: 

                 367     347     255
 
BlpI GC'TnA_GC

Cuts at:      0     847     969 
   Size:        847     122
 
Bme1580I G_kGCm'C

Cuts at:      0     108     613     728     969 
   Size:        108     505     115     241

  Fragments arranged by size: 

                 505     241     115     108
 
BmgBI CAC'GTC

Cuts at:      0     378     969 
   Size:        378     591
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     234     969 
   Size:        234     735
 
Bpu10I CC'TnA_GC

Cuts at:      0     587     969 
   Size:        587     382
 
BsaBI GATnn'nnATC

Cuts at:      0     191     969 
   Size:        191     778
 
BsaHI Gr'CG_yC

Cuts at:      0     818     939     969 
   Size:        818     121      30

  Fragments arranged by size: 

                 818     121      30
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     895     925     969 
   Size:        895      30      44

  Fragments arranged by size: 

                 895      44      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     127     130     250     271     274     969 
   Size:        127       3     120      21       3     695

  Fragments arranged by size: 

                 695     127     120      21       3       3
 
BseYI C'CCAG_C

Cuts at:      0      38      95     175     292     717     821     826     969 
   Size:         38      57      80     117     425     104       5     143

  Fragments arranged by size: 

                 425     143     117     104      80      57      38       5
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     358     370     969 
   Size:        349       9      12     599

  Fragments arranged by size: 

                 599     349      12       9
 
BsiEI CG_ry'CG

Cuts at:      0     516     732     969 
   Size:        516     216     237

  Fragments arranged by size: 

                 516     237     216
 
BsiHKAI G_wGCw'C

Cuts at:      0     512     613     728     815     969 
   Size:        512     101     115      87     154

  Fragments arranged by size: 

                 512     154     115     101      87
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     946     969 
   Size:        946      23
 
Bsp1286I G_dGCh'C

Cuts at:      0      66     108     512     613     728     815     969 
   Size:         66      42     404     101     115      87     154

  Fragments arranged by size: 

                 404     154     115     101      87      66      42
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     686     969 
   Size:        686     283
 
BsrBI CCG'CTC

Cuts at:      0     120     846     969 
   Size:        120     726     123

  Fragments arranged by size: 

                 726     123     120
 
BsrFI r'CCGG_y

Cuts at:      0     346     639     969 
   Size:        346     293     330

  Fragments arranged by size: 

                 346     330     293
 
BssHII G'CGCG_C

Cuts at:      0     681     735     969 
   Size:        681      54     234

  Fragments arranged by size: 

                 681     234      54
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     372     477     969 
   Size:        372     105     492

  Fragments arranged by size: 

                 492     372     105
 
BstBI TT'CG_AA

Cuts at:      0     581     758     969 
   Size:        581     177     211

  Fragments arranged by size: 

                 581     211     177
 
Bsu36I CC'TnA_GG

Cuts at:      0     311     969 
   Size:        311     658
 
BtgI C'CryG_G

Cuts at:      0      57     969 
   Size:         57     912
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     476     778     969 
   Size:        476     302     191

  Fragments arranged by size: 

                 476     302     191
 
EaeI y'GGCC_r

Cuts at:      0      54     344     513     765     969 
   Size:         54     290     169     252     204

  Fragments arranged by size: 

                 290     252     204     169      54
 
EagI C'GGCC_G

Cuts at:      0     513     969 
   Size:        513     456
 
EarI CTCTTCn'nnn_

Cuts at:      0     248     760     969 
   Size:        248     512     209

  Fragments arranged by size: 

                 512     248     209
 
EcoICRI GAG'CTC

Cuts at:      0     813     969 
   Size:        813     156
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     163     553     969 
   Size:        163     390     416

  Fragments arranged by size: 

                 416     390     163
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     481     969 
   Size:        481     488
 
EcoO109I rG'GnC_Cy

Cuts at:      0     132     744     969 
   Size:        132     612     225

  Fragments arranged by size: 

                 612     225     132
 
FseI GG_CCGG'CC

Cuts at:      0     350     969 
   Size:        350     619
 
HaeII r_GCGC'y

Cuts at:      0     667     691     821     833     969 
   Size:        667      24     130      12     136

  Fragments arranged by size: 

                 667     136     130      24      12
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     895     927     969 
   Size:        895      32      42

  Fragments arranged by size: 

                 895      42      32
 
KasI G'GCGC_C

Cuts at:      0     817     969 
   Size:        817     152
 
KpnI G_GTAC'C

Cuts at:      0     678     969 
   Size:        678     291
 
MluI A'CGCG_T

Cuts at:      0     389     969 
   Size:        389     580
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     374     969 
   Size:        374     595
 
MscI TGG'CCA

Cuts at:      0      56     969 
   Size:         56     913
 
MslI CAynn'nnrTG

Cuts at:      0     403     969 
   Size:        403     566
 
MspA1I CmG'CkG

Cuts at:      0     372     435     936     969 
   Size:        372      63     501      33

  Fragments arranged by size: 

                 501     372      63      33
 
NaeI GCC'GGC

Cuts at:      0     348     641     969 
   Size:        348     293     328

  Fragments arranged by size: 

                 348     328     293
 
NarI GG'CG_CC

Cuts at:      0     818     969 
   Size:        818     151
 
NcoI C'CATG_G

Cuts at:      0      57     969 
   Size:         57     912
 
NgoMIV G'CCGG_C

Cuts at:      0     346     639     969 
   Size:        346     293     330

  Fragments arranged by size: 

                 346     330     293
 
PasI CC'CwG_GG

Cuts at:      0     420     969 
   Size:        420     549
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      45     657     969 
   Size:         45     612     312

  Fragments arranged by size: 

                 612     312      45
 
PstI C_TGCA'G

Cuts at:      0     155     482     969 
   Size:        155     327     487

  Fragments arranged by size: 

                 487     327     155
 
PvuI CG_AT'CG

Cuts at:      0     732     969 
   Size:        732     237
 
PvuII CAG'CTG

Cuts at:      0     372     435     969 
   Size:        372      63     534

  Fragments arranged by size: 

                 534     372      63
 
SacI G_AGCT'C

Cuts at:      0     815     969 
   Size:        815     154
 
SbfI CC_TGCA'GG

Cuts at:      0     482     969 
   Size:        482     487
 
SfcI C'TryA_G

Cuts at:      0     151     478     969 
   Size:        151     327     491

  Fragments arranged by size: 

                 491     327     151
 
SfoI GGC'GCC

Cuts at:      0     819     969 
   Size:        819     150
 
SmlI C'TyrA_G

Cuts at:      0     877     969 
   Size:        877      92
 
StuI AGG'CCT

Cuts at:      0     362     898     969 
   Size:        362     536      71

  Fragments arranged by size: 

                 536     362      71
 
StyI C'CwwG_G

Cuts at:      0      57     162     969 
   Size:         57     105     807

  Fragments arranged by size: 

                 807     105      57
 
Tth111I GACn'n_nGTC

Cuts at:      0     319     969 
   Size:        319     650
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     865     969 
   Size:        865     104
 
XhoI C'TCGA_G

Cuts at:      0     877     969 
   Size:        877      92
 

 Enzymes that do cut:

   Acc65I     AcuI   AflIII     AleI    AlwNI    ApaLI     AscI     AvaI
    AvrII     BanI    BanII     BbeI     BglI     BlpI Bme1580I    BmgBI
     BmrI   Bpu10I    BsaBI    BsaHI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI   BssHII
   BstAPI    BstBI   Bsu36I     BtgI    BtgZI     EaeI     EagI     EarI
  EcoICRI  Eco57MI    EcoNI EcoO109I     FseI    HaeII    Hin4I     KasI
     KpnI     MluI     MmeI     MscI     MslI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     PasI    PflMI     PstI     PvuI    PvuII     SacI
     SbfI     SfcI     SfoI     SmlI     StuI     StyI  Tth111I     XcmI
     XhoI

 Enzymes that do not cut: 

     AarI    AatII     AccI     AclI     AfeI    AflII     AgeI     AhdI
     AlfI     AloI     ApaI     ApoI     AseI    AsiSI     BaeI    BamHI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BmtI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaWI    BsiWI     BsmI
    BspEI    BspHI    BsrDI    BsrGI    BssSI   BstEII    BstXI    BstYI
  BstZ17I     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EciI
    EcoRI    EcoRV     FalI     FspI    FspAI   HincII  HindIII     HpaI
     MfeI     NdeI     NheI     NotI     NruI     NsiI     NspI     PacI
     PciI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI    RsrII    SacII     SalI    SanDI     SapI
     ScaI    SexAI     SfiI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TatI     TstI     XbaI
     XmaI     XmnI     ZraI
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