Restriction map for pF1KF0040
##################################
# NO CUT ENZYMES (   87 enzymes) #
##################################

AatII       AccIII      AclI        AflIII      AgeI        
Alw44I      AocI        ApaLI       AscI        AseI        
AsnI        AspHI       AvaI        AviII       AvrII       
BamHI       BbrPI       BbvCI       BcgI        BclI        
BlnI        BseAI       BsiHKAI     BsiWI       BsoBI       
Bsp1407I    BspDI       BspEI       BspLU11I    BsrGI       
BssHII      Bst1107I    BstAPI      BstZ17I     Bsu36I      
ClaI        CpoI        DraIII      Eco81I      EcoRV       
EcoT22I     FbaI        FseI        FspI        HincII      
HindII      HpaI        KspI        MfeI        MluI        
MroI        MslI        MunI        NdeI        NheI        
NotI        NruI        NsiI        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacI        
SacII       SalI        SbfI        ScaI        SexAI       
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        SwaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

Acc65I       2685  GGTACC               G^GTACC
AccII        1563  CGCG                 CG^CG
AflII        1147  CTTAAG               C^TTAAG
AhdI         1654  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI         3093  GGGCCC               GGGCC^C
Asp718I      2685  GGTACC               G^GTACC
AspEI        1654  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII        3093  GGGCCC               G[GA]GC[CT]^C
BbeI         1456  GGCGCC               GGCGC^C
BciVI        1045  GGATAC               GTATCC(6/5)
BfaI         2044  CTAG                 C^TAG
BfrI         1147  CTTAAG               C^TTAAG
BlpI          814  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      814  GCTCAGC              GC^T[ACGT]AGC
BsaAI         679  TACGTG               [CT]AC^GT[GA]
BsaBI        2035  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI         651  TCATGA               T^CATGA
BsrFI        1966  GCCGGC               [GA]^CCGG[CT]
BstEII       1222  GGTAACC              G^GT[ACGT]ACC
BstPI        1222  GGTAACC              G^GT[ACGT]ACC
BstUI        1563  CGCG                 CG^CG
CelII         814  GCTCAGC              GC^T[ACGT]AGC
Cfr10I       1966  GCCGGC               [GA]^CCGG[CT]
DraI          673  TTTAAA               TTT^AAA
DrdI         2326  GACCAAGCCGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI         2262  CGGCCG               C^GGCCG
Eam1105I     1654  GACGTGGAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2262  CGGCCG               C^GGCCG
Eco52I       2262  CGGCCG               C^GGCCG
EcoNI        1859  CCTTCGAGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I      1222  GGTAACC              G^GT[ACGT]ACC
HindIII       847  AAGCTT               A^AGCTT
KasI         1456  GGCGCC               G^GCGCC
KpnI         2685  GGTACC               GGTAC^C
MaeI         2044  CTAG                 C^TAG
MamI         2035  GATGGAGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         1563  CGCG                 CG^CG
NaeI         1966  GCCGGC               GCC^GGC
NarI         1456  GGCGCC               GG^CGCC
NgoMIV       1966  GCCGGC               G^CCGGC
NspI         2244  GCATGT               [GA]CATG^[CT]
PflMI         342  CCATCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI         1753  CTGCAG               CTGCA^G
RcaI          651  TCATGA               T^CATGA
SfiI         3005  GGCCAGAGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI         1456  GGCGCC               GGC^GCC
StuI         3013  AGGCCT               AGG^CCT
Van91I        342  CCATCCTTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         2043  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   27 enzymes) #
###################################

AccI          144  GTCTAC               GT^[AC][GT]AC
             2638  GTAGAC              
AfaI          109  GTAC                 GT^AC
             2686  GTAC                
Aor51HI      1907  AGCGCT               AGC^GCT
             2535  AGCGCT              
AspI         1179  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2101  GACACTGTC           
BalI         2460  TGGCCA               TGG^CCA
             3004  TGGCCA              
BglII         741  AGATCT               A^GATCT
             2040  AGATCT              
BsaI          913  GAGACC               GGTCTC(1/5)
             1984  GAGACC              
BsaWI         771  ACCGGA               [AT]^CCGG[AT]
             2455  TCCGGT              
BsiEI        2262  CGGCCG               CG[GA][CT]^CG
             3080  CGGTCG              
BspMI        2954  ACCTGC               ACCTGC(4/8)
             2308  GCAGGT              
BsrBI        1495  CCGCTC               CCGCTC(-3/-3)
             2338  CCGCTC              
BstBI        1063  TTCGAA               TT^CGAA
             2394  TTCGAA              
BstXI          87  CCAAATCCATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             2826  CCACTCAGCTGG        
Eco47III     1907  AGCGCT               AGC^GCT
             2535  AGCGCT              
EcoRI        2125  GAATTC               G^AATTC
             2655  GAATTC              
MluNI        2460  TGGCCA               TGG^CCA
             3004  TGGCCA              
MscI         2460  TGGCCA               TGG^CCA
             3004  TGGCCA              
NspV         1063  TTCGAA               TT^CGAA
             2394  TTCGAA              
PflFI        1179  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2101  GACACTGTC           
PpuMI        2081  AGGACCT              [GA]G^G[AT]CC[CT]
             2417  AGGACCT             
PvuII         817  CAGCTG               CAG^CTG
             2831  CAGCTG              
RsaI          109  GTAC                 GT^AC
             2686  GTAC                
SapI          184  GAAGAGC              GCTCTTC(1/4)
              370  GAAGAGC             
SfuI         1063  TTCGAA               TT^CGAA
             2394  TTCGAA              
TfiI         1827  GATTC                G^A[AT]TC
             2392  GATTC               
Tth111I      1179  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2101  GACACTGTC           
XcmI          800  CCAAAAGAGAGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1451  CCAAGGGCGCCATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   95 enzymes) #
#########################################

AciI        AcsI        AcyI        AluI        AlwI        
AlwNI       ApoI        Asp700I     AvaII       BanI        
BbsI        BbvI        BcnI        BglI        BmyI        
BpmI        BpuAI       BsaHI       BsaJI       BseRI       
BsgI        BsiYI       BslI        BsmAI       BsmBI       
BsmFI       BsmI        Bsp1286I    BsrDI       BsrI        
BssKI       BssSI       BstF5I      BstNI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EarI        EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MspA1I      
MspI        MvaI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       PleI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
StyI        TaqI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       XmnI        
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