Restriction map for pF1KF0040
 (Linear) (Six-base) MAPSORT of: FLJ00040.seq  Check: 4592  from: 1  to: 3150



FLJ00040.orf

 With 182 enzymes: SgfI * 

                        March 2, 2010 14:32  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    2299    3150 
   Size:       2299     851
 
AccI GT'mk_AC

Cuts at:      0     145    2639    3150 
   Size:        145    2494     511

  Fragments arranged by size: 

                2494     511     145
 
Acc65I G'GTAC_C

Cuts at:      0    2685    3150 
   Size:       2685     465
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     730     996    1810    2311    2662    3150 
   Size:        730     266     814     501     351     488

  Fragments arranged by size: 

                 814     730     501     488     351     266
 
AfeI AGC'GCT

Cuts at:      0    1909    2537    3150 
   Size:       1909     628     613

  Fragments arranged by size: 

                1909     628     613
 
AflII C'TTAA_G

Cuts at:      0    1147    3150 
   Size:       1147    2003
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1659    3150 
   Size:       1659    1491
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1750    1782    2588    2620    3150 
   Size:       1750      32     806      32     530

  Fragments arranged by size: 

                1750     806     530      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     567    2032    2613    3002    3150 
   Size:        567    1465     581     389     148

  Fragments arranged by size: 

                1465     581     567     389     148
 
ApaI G_GGCC'C

Cuts at:      0    3097    3150 
   Size:       3097      53
 
ApoI r'AATT_y

Cuts at:      0     656     990    2125    2655    3150 
   Size:        656     334    1135     530     495

  Fragments arranged by size: 

                1135     656     530     495     334
 
BanI G'GyrC_C

Cuts at:      0     381    1456    2311    2685    3150 
   Size:        381    1075     855     374     465

  Fragments arranged by size: 

                1075     855     465     381     374
 
BanII G_rGCy'C

Cuts at:      0    3097    3150 
   Size:       3097      53
 
BbeI G_GCGC'C

Cuts at:      0    1460    3150 
   Size:       1460    1690
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      23     256     444    2559    3150 
   Size:         23     233     188    2115     591

  Fragments arranged by size: 

                2115     591     233     188      23
 
BciVI GTATCCnnnnn_n'

Cuts at:      0    1039    3150 
   Size:       1039    2111
 
BglI GCCn_nnn'nGGC

Cuts at:      0    2228    2360    2925    3012    3150 
   Size:       2228     132     565      87     138

  Fragments arranged by size: 

                2228     565     138     132      87
 
BglII A'GATC_T

Cuts at:      0     741    2040    3150 
   Size:        741    1299    1110

  Fragments arranged by size: 

                1299    1110     741
 
BlpI GC'TnA_GC

Cuts at:      0     815    3150 
   Size:        815    2335
 
Bme1580I G_kGCm'C

Cuts at:      0     278    1391    2152    2163    3097    3150 
   Size:        278    1113     761      11     934      53

  Fragments arranged by size: 

                1113     934     761     278      53      11
 
BmgBI CAC'GTC

Cuts at:      0    1656    2565    3150 
   Size:       1656     909     585

  Fragments arranged by size: 

                1656     909     585
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1051    3150 
   Size:       1051    2099
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     593    2990    3150 
   Size:        593    2397     160

  Fragments arranged by size: 

                2397     593     160
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     931    1356    2107    3150 
   Size:        541     390     425     751    1043

  Fragments arranged by size: 

                1043     751     541     425     390
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1789    1956    3150 
   Size:       1789     167    1194

  Fragments arranged by size: 

                1789    1194     167
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     907    1978    3150 
   Size:        907    1071    1172

  Fragments arranged by size: 

                1172    1071     907
 
BsaAI yAC'GTr

Cuts at:      0     681    3150 
   Size:        681    2469
 
BsaBI GATnn'nnATC

Cuts at:      0    2039    3150 
   Size:       2039    1111
 
BsaHI Gr'CG_yC

Cuts at:      0    1457    1736    1798    3150 
   Size:       1457     279      62    1352

  Fragments arranged by size: 

                1457    1352     279      62
 
BsaWI w'CCGG_w

Cuts at:      0     771    2455    3150 
   Size:        771    1684     695

  Fragments arranged by size: 

                1684     771     695
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     873     903     904     934    1170    1200    2218    2248 
   Size:        873      30       1      30     236      30    1018      30

Cuts at:   2248    2437    2467    2485    2515    2588    2618    2916    2946 
   Size:        189      30      18      30      73      30     298      30

Cuts at:   2946    3150 
   Size:        204

  Fragments arranged by size: 

                1018     873     298     236     204     189      73      30
                  30      30      30      30      30      30      30      18
                   1
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1881    1944    1996    2092    2223    3150 
   Size:       1881      63      52      96     131     927

  Fragments arranged by size: 

                1881     927     131      96      63      52
 
BseYI C'CCAG_C

Cuts at:      0    1721    2163    3150 
   Size:       1721     442     987

  Fragments arranged by size: 

                1721     987     442
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      93     892    1531    1591    1593    1686    1915    2132 
   Size:         93     799     639      60       2      93     229     217

Cuts at:   2132    2242    2524    3150 
   Size:        110     282     626

  Fragments arranged by size: 

                 799     639     626     282     229     217     110      93
                  93      60       2
 
BsiEI CG_ry'CG

Cuts at:      0    2265    3083    3150 
   Size:       2265     818      67

  Fragments arranged by size: 

                2265     818      67
 
BsmI GAATG_Cn'

Cuts at:      0     499     540    2403    3150 
   Size:        499      41    1863     747

  Fragments arranged by size: 

                1863     747     499      41
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0    1190    1335    1370    1789    3150 
   Size:       1190     145      35     419    1361

  Fragments arranged by size: 

                1361    1190     419     145      35
 
Bsp1286I G_dGCh'C

Cuts at:      0     278    1391    2152    2163    3097    3150 
   Size:        278    1113     761      11     934      53

  Fragments arranged by size: 

                1113     934     761     278      53      11
 
BspHI T'CATG_A

Cuts at:      0     651    3150 
   Size:        651    2499
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    2299    2963    3150 
   Size:       2299     664     187

  Fragments arranged by size: 

                2299     664     187
 
BsrBI CCG'CTC

Cuts at:      0    1497    2340    3150 
   Size:       1497     843     810

  Fragments arranged by size: 

                1497     843     810
 
BsrDI GCAATG_nn'

Cuts at:      0    1211    1689    1709    1747    3150 
   Size:       1211     478      20      38    1403

  Fragments arranged by size: 

                1403    1211     478      38      20
 
BsrFI r'CCGG_y

Cuts at:      0    1966    3150 
   Size:       1966    1184
 
BssSI C'ACGA_G

Cuts at:      0     794    1621    2467    2542    3150 
   Size:        794     827     846      75     608

  Fragments arranged by size: 

                 846     827     794     608      75
 
BstBI TT'CG_AA

Cuts at:      0    1064    2395    3150 
   Size:       1064    1331     755

  Fragments arranged by size: 

                1331    1064     755
 
BstEII G'GTnAC_C

Cuts at:      0    1222    3150 
   Size:       1222    1928
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0      94    2833    3150 
   Size:         94    2739     317

  Fragments arranged by size: 

                2739     317      94
 
BstYI r'GATC_y

Cuts at:      0     390     741     971    1775    2040    2971    3150 
   Size:        390     351     230     804     265     931     179

  Fragments arranged by size: 

                 931     804     390     351     265     230     179
 
BtgI C'CryG_G

Cuts at:      0      93     689    1460    1482    1913    2592    3150 
   Size:         93     596     771      22     431     679     558

  Fragments arranged by size: 

                 771     679     596     558     431      93      22
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     450    1325    3150 
   Size:        450     875    1825

  Fragments arranged by size: 

                1825     875     450
 
BtsI GCAGTG_nn'

Cuts at:      0     638    2479    3150 
   Size:        638    1841     671

  Fragments arranged by size: 

                1841     671     638
 
DraI TTT'AAA

Cuts at:      0     675    3150 
   Size:        675    2475
 
DrdI GACnn_nn'nnGTC

Cuts at:      0    2332    3150 
   Size:       2332     818
 
EaeI y'GGCC_r

Cuts at:      0    1617    2262    2460    3004    3150 
   Size:       1617     645     198     544     146

  Fragments arranged by size: 

                1617     645     544     198     146
 
EagI C'GGCC_G

Cuts at:      0    2262    3150 
   Size:       2262     888
 
EarI CTCTTCn'nnn_

Cuts at:      0     179     308     365    2167    3150 
   Size:        179     129      57    1802     983

  Fragments arranged by size: 

                1802     983     179     129      57
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    2269    2284    3150 
   Size:       2269      15     866

  Fragments arranged by size: 

                2269     866      15
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     541     730     931     996    1356    1810    2107    2311 
   Size:        541     189     201      65     360     454     297     204

Cuts at:   2311    2662    3150 
   Size:        351     488

  Fragments arranged by size: 

                 541     488     454     360     351     297     204     201
                 189      65
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1863    3150 
   Size:       1863    1287
 
EcoO109I rG'GnC_Cy

Cuts at:      0    1887    2082    2418    2498    3093    3150 
   Size:       1887     195     336      80     595      57

  Fragments arranged by size: 

                1887     595     336     195      80      57
 
EcoRI G'AATT_C

Cuts at:      0    2125    2655    3150 
   Size:       2125     530     495

  Fragments arranged by size: 

                2125     530     495
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     234    2583    3150 
   Size:        234    2349     567

  Fragments arranged by size: 

                2349     567     234
 
HaeII r_GCGC'y

Cuts at:      0    1460    1911    2539    3150 
   Size:       1460     451     628     611

  Fragments arranged by size: 

                1460     628     611     451
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     498     530     922     954    1170    1202    1645    1677 
   Size:        498      32     392      32     216      32     443      32

Cuts at:   1677    2218    2250    3150 
   Size:        541      32     900

  Fragments arranged by size: 

                 900     541     498     443     392     216      32      32
                  32      32      32
 
HindIII A'AGCT_T

Cuts at:      0     847    3150 
   Size:        847    2303
 
KasI G'GCGC_C

Cuts at:      0    1456    3150 
   Size:       1456    1694
 
KpnI G_GTAC'C

Cuts at:      0    2689    3150 
   Size:       2689     461
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    2045    3150 
   Size:       2045    1105
 
MscI TGG'CCA

Cuts at:      0    2462    3006    3150 
   Size:       2462     544     144

  Fragments arranged by size: 

                2462     544     144
 
MspA1I CmG'CkG

Cuts at:      0     819    1721    2241    2833    3150 
   Size:        819     902     520     592     317

  Fragments arranged by size: 

                 902     819     592     520     317
 
NaeI GCC'GGC

Cuts at:      0    1968    3150 
   Size:       1968    1182
 
NarI GG'CG_CC

Cuts at:      0    1457    3150 
   Size:       1457    1693
 
NcoI C'CATG_G

Cuts at:      0      93     689    1460    1482    3150 
   Size:         93     596     771      22    1668

  Fragments arranged by size: 

                1668     771     596      93      22
 
NgoMIV G'CCGG_C

Cuts at:      0    1966    3150 
   Size:       1966    1184
 
NspI r_CATG'y

Cuts at:      0    2248    3150 
   Size:       2248     902
 
PasI CC'CwG_GG

Cuts at:      0    3090    3150 
   Size:       3090      60
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     348    3150 
   Size:        348    2802
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     348     380     454     486    2588    2620    3150 
   Size:        348      32      74      32    2102      32     530

  Fragments arranged by size: 

                2102     530     348      74      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0    2082    2418    3150 
   Size:       2082     336     732

  Fragments arranged by size: 

                2082     732     336
 
PspOMI G'GGCC_C

Cuts at:      0    3093    3150 
   Size:       3093      57
 
PstI C_TGCA'G

Cuts at:      0    1757    3150 
   Size:       1757    1393
 
PvuII CAG'CTG

Cuts at:      0     819    2833    3150 
   Size:        819    2014     317

  Fragments arranged by size: 

                2014     819     317
 
SapI GCTCTTCn'nnn_

Cuts at:      0     179     365    3150 
   Size:        179     186    2785

  Fragments arranged by size: 

                2785     186     179
 
SfcI C'TryA_G

Cuts at:      0    1753    1998    2488    2636    3150 
   Size:       1753     245     490     148     514

  Fragments arranged by size: 

                1753     514     490     245     148
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    3012    3150 
   Size:       3012     138
 
SfoI GGC'GCC

Cuts at:      0    1458    3150 
   Size:       1458    1692
 
SmlI C'TyrA_G

Cuts at:      0    1147    1804    1971    3150 
   Size:       1147     657     167    1179

  Fragments arranged by size: 

                1179    1147     657     167
 
StuI AGG'CCT

Cuts at:      0    3015    3150 
   Size:       3015     135
 
StyI C'CwwG_G

Cuts at:      0      93     689    1451    1460    1482    1731    3150 
   Size:         93     596     762       9      22     249    1419

  Fragments arranged by size: 

                1419     762     596     249      93      22       9
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     904     936    2485    2517    2916    2948    3150 
   Size:        904      32    1549      32     399      32     202

  Fragments arranged by size: 

                1549     904     399     202      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:      0    1182    2104    3150 
   Size:       1182     922    1046

  Fragments arranged by size: 

                1182    1046     922
 
XbaI T'CTAG_A

Cuts at:      0    2043    3150 
   Size:       2043    1107
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     807    1458    3150 
   Size:        807     651    1692

  Fragments arranged by size: 

                1692     807     651
 
XmnI GAAnn'nnTTC

Cuts at:      0    1082    2675    2853    3150 
   Size:       1082    1593     178     297

  Fragments arranged by size: 

                1593    1082     297     178
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AcuI     AfeI    AflII     AhdI     AloI
    AlwNI     ApaI     ApoI     BanI    BanII     BbeI     BbsI    BciVI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI     BsmI    BsmBI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI    BstBI   BstEII    BstXI    BstYI
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII    Hin4I
  HindIII     KasI     KpnI     MmeI     MscI   MspA1I     NaeI     NarI
     NcoI   NgoMIV     NspI     PasI    PflMI     PpiI    PpuMI   PspOMI
     PstI    PvuII     SapI     SfcI     SfiI     SfoI     SmlI     StuI
     StyI     TstI  Tth111I     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AclI   AflIII     AgeI     AleI     AlfI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI    BbvCI     BcgI
     BclI    BfrBI     BmtI   Bpu10I  BsiHKAI    BsiWI    BspEI    BsrGI
   BssHII   BstAPI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII  EcoICRI
    EcoRV     FseI     FspI    FspAI   HincII     HpaI     MfeI     MluI
     MslI     NdeI     NheI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PmlI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII     SacI    SacII     SalI    SanDI     SbfI     ScaI    SexAI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI    TaqII     TatI     XhoI     XmaI     ZraI
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