Restriction map for pF1KB5019
##################################
# NO CUT ENZYMES (  146 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        ApaI        
ApaLI       AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspI        AviII       BalI        
BamHI       BanI        BbeI        BbrPI       BbvCI       
BcgI        BciVI       BclI        BfrI        BglI        
BglII       BsaAI       BsaHI       BsaI        BsaWI       
BseAI       BsgI        BsiEI       BsiWI       BsmBI       
BsmI        Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstPI       BstXI       BstZ17I     Bsu36I      Cfr10I      
ClaI        CpoI        DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
KasI        KpnI        MaeIII      MfeI        MluI        
MluNI       MroI        MscI        MunI        NaeI        
NarI        NcoI        NgoMIV      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SalI        SapI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
SphI        SspBI       SspI        StuI        SwaI        
Tsp45I      Tth111I     XbaI        XcmI        XhoI        
XmnI        


###################################
# ONE CUT ENZYMES (   49 enzymes) #
###################################

AlwI         1509  GGATC                GGATC(4/5)
Aor51HI        18  AGCGCT               AGC^GCT
AspHI         951  GAGCAC               G[AT]GC[AT]^C
AvaI           27  CCCGGG               C^[CT]CG[GA]G
AvrII         352  CCTAGG               C^CTAGG
BbsI          745  GAAGAC               GAAGAC(2/6)
BlnI          352  CCTAGG               C^CTAGG
BlpI         1266  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I     1266  GCTCAGC              GC^T[ACGT]AGC
BpuAI         745  GAAGAC               GAAGAC(2/6)
BsaBI        1072  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI        1018  GAGGAG               GAGGAG(10/8)
BsiHKAI       951  GAGCAC               G[AT]GC[AT]^C
BsmFI         232  GTCCC                GGGAC(10/14)
BsoBI          27  CCCGGG               C^[CT]CG[GA]G
BspMI         334  GCAGGT               ACCTGC(4/8)
BsrDI        1118  GCAATG               GCAATG(2/0)
BstYI        1509  GGATCT               [GA]^GATC[CT]
CelII        1266  GCTCAGC              GC^T[ACGT]AGC
DpnII        1510  GATC                 ^GATC
DsaI           50  CCGCGG               C^C[GA][CT]GG
EaeI         1407  TGGCCG               [CT]^GGCC[GA]
EarI         1083  GAAGAG               CTCTTC(1/4)
Eco47III       18  AGCGCT               AGC^GCT
EcoRV         296  GATATC               GAT^ATC
HapII          28  CCGG                 C^CGG
HpaII          28  CCGG                 C^CGG
Ksp632I      1083  GAAGAG               CTCTTC(1/4)
KspI           50  CCGCGG               CCGC^GG
MaeII          77  ACGT                 A^CGT
MamI         1072  GATGGCAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI         1510  GATC                 ^GATC
MflI         1509  GGATCT               [GA]^GATC[CT]
MslI          556  CATACGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           28  CCGG                 C^CGG
NdeI         1331  CATATG               CA^TATG
NdeII        1510  GATC                 ^GATC
NheI          904  GCTAGC               G^CTAGC
PflMI         827  CCAACTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII          50  CCGCGG               CCGC^GG
Sau3AI       1510  GATC                 ^GATC
SbfI         1056  CCTGCAGG             CCTGCA^GG
SmaI           27  CCCGGG               CCC^GGG
SmlI          531  CTTGAG               C^T[CT][GA]AG
Sse8387I     1056  CCTGCAGG             CCTGCA^GG
Van91I        827  CCAACTACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1509  GGATCT               [GA]^GATC[CT]
XmaCI          27  CCCGGG               C^CCGGG
XmaI           27  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AccII          51  CGCG                 CG^CG
             1028  CGCG                
AvaII         349  GGACC                G^G[AT]CC
              397  GGACC               
BanII         126  GGGCTC               G[GA]GC[CT]^C
             1237  GAGCCC              
BcnI           27  CCCGG                CC^[GC]GG
               28  CCGGG               
BsmAI         183  GAGAC                GTCTC(1/5)
              570  GTCTC               
BsrI          833  ACTGG                ACTGG(1/-1)
              683  CCAGT               
BstF5I        873  GGATG                GGATG(2/0)
             1302  GGATG               
BstUI          51  CGCG                 CG^CG
             1028  CGCG                
EcoT14I       352  CCTAGG               C^C[AT][AT]GG
             1210  CCTTGG              
FauI           56  CCCGC                CCCGC(4/6)
              133  GCGGG               
FokI          873  GGATG                GGATG(9/13)
             1302  GGATG               
HaeII          18  AGCGCT               [GA]GCGC^[CT]
               36  GGCGCT              
HphI          325  GGTGA                GGTGA(8/7)
              576  GGTGA               
MspA1I         50  CCGCGG               C[AC]G^C[GT]G
               65  CCGCTG              
MvnI           51  CGCG                 CG^CG
             1028  CGCG                
NciI           27  CCCGG                CC^[GC]GG
               28  CCGGG               
PstI          206  CTGCAG               CTGCA^G
             1057  CTGCAG              
StyI          352  CCTAGG               C^C[AT][AT]GG
             1210  CCTTGG              
TfiI          484  GATTC                G^A[AT]TC
              966  GATTC               


#########################################
# 3 OR MORE CUT ENZYMES (   44 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BbvI        
BfaI        BmyI        BpmI        BsaJI       BsiYI       
BslI        Bsp1286I    BssKI       BstNI       Cac8I       
CfoI        Cfr13I      DdeI        EcoRII      Fnu4HI      
HaeIII      HhaI        HinP1I      HinfI       ItaI        
MaeI        MboII       MnlI        MseI        MvaI        
MwoI        NlaIII      NlaIV       PleI        Sau96I      
ScrFI       SfaNI       SfcI        TaqI        Tru9I       
TseI        Tsp509I     TspRI       TthHB8I     
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