Restriction map for pF1KB3466
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp718I     AspEI       
AspHI       AviII       AvrII       BanI        BbeI        
BbrPI       BbvCI       BciVI       BclI        BfrI        
BlnI        BsaAI       BsaBI       BsaHI       BsaWI       
BseAI       BsiEI       BsiHKAI     BsiWI       BsmBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrDI       BsrFI       BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Cfr10I      ClaI        
CpoI        DraI        DraIII      DrdI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      EcoNI       
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        Hin1I       HincII      HindII      
HindIII     HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacI        
SacII       SalI        SapI        SbfI        ScaI        
SfoI        SfuI        SgrAI       SnaBI       SpeI        
Sse8387I    SspBI       SspI        SwaI        Van91I      
XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AocI          256  CCTGAGG              CC^T[ACGT]AGG
AspI          796  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BamHI         512  GGATCC               G^GATCC
BanII        1112  GGGCTC               G[GA]GC[CT]^C
BcgI           11  CGATCAGTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI         1469  CTAG                 C^TAG
BglI         1195  GCCAAGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         588  AGATCT               A^GATCT
BsmFI        1223  GGGAC                GGGAC(10/14)
BsmI          326  GCATTC               GAATGC(1/-1)
BsrBI         508  GAGCGG               CCGCTC(-3/-3)
BstAPI       1417  GCAGAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I        256  CCTGAGG              CC^T[ACGT]AGG
DraII        1568  AGGCCCT              [GA]G^G[ACGT]CC[CT]
EarI         1189  GAAGAG               CTCTTC(1/4)
Eco81I        256  CCTGAGG              CC^T[ACGT]AGG
EcoO109I     1568  AGGCCCT              [GA]G^G[ACGT]CC[CT]
HaeII         296  AGCGCC               [GA]GCGC^[CT]
HgaI         1176  GCGTC                GACGC(5/10)
Ksp632I      1189  GAAGAG               CTCTTC(1/4)
MaeI         1469  CTAG                 C^TAG
MaeII        1521  ACGT                 A^CGT
MslI          897  CATTGAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI           32  CCATGG               C^CATGG
PflFI         796  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SexAI          92  ACCAGGT              A^CC[AT]GGT
SfiI         1194  GGCCAAGGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI          542  GCATGC               GCATG^C
Tth111I       796  GACTTCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          390  CCAGTCTAAGTATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   31 enzymes) #
###################################

AcsI          760  AAATTT               [GA]^AATT[CT]
             1717  GAATTT              
AlwNI         295  CAGCGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1418  CAGAGCCTG           
ApoI          760  AAATTT               [GA]^AATT[CT]
             1717  GAATTT              
Asp700I       274  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              609  GAACTACTTC          
AvaI          926  CCCGGG               C^[CT]CG[GA]G
             1220  CCCGGG              
BlpI         1081  GCTCAGC              GC^T[ACGT]AGC
             1381  GCTGAGC             
BmyI         1112  GGGCTC               G[AGT]GC[ACT]^C
             1142  GTGCCC              
Bpu1102I     1081  GCTCAGC              GC^T[ACGT]AGC
             1381  GCTGAGC             
BsaI          358  GAGACC               GGTCTC(1/5)
              670  GGTCTC              
BsgI          904  GTGCAG               GTGCAG(16/14)
             1552  GTGCAG              
BsoBI         926  CCCGGG               C^[CT]CG[GA]G
             1220  CCCGGG              
Bsp1286I     1112  GGGCTC               G[AGT]GC[ACT]^C
             1142  GTGCCC              
CelII        1081  GCTCAGC              GC^T[ACGT]AGC
             1381  GCTGAGC             
DsaI           32  CCATGG               C^C[GA][CT]GG
              537  CCGTGG              
FauI          351  CCCGC                CCCGC(4/6)
              459  CCCGC               
HphI           99  GGTGA                GGTGA(8/7)
              192  GGTGA               
MaeIII         25  GTGAC                ^GT[ACGT]AC
              193  GTGAC               
MseI          233  TTAA                 T^TAA
              827  TTAA                
PstI          139  CTGCAG               CTGCA^G
             1210  CTGCAG              
PvuII        1246  CAGCTG               CAG^CTG
             1288  CAGCTG              
SfcI          139  CTGCAG               C^T[GA][CT]AG
             1210  CTGCAG              
SmaI          926  CCCGGG               CCC^GGG
             1220  CCCGGG              
StuI         1202  AGGCCT               AGG^CCT
             1214  AGGCCT              
TaqI         1061  TCGA                 T^CGA
             1278  TCGA                
TfiI          279  GATTC                G^A[AT]TC
             1280  GAATC               
Tru9I         233  TTAA                 T^TAA
              827  TTAA                
Tsp45I         25  GTGAC                ^GT[GC]AC
              193  GTGAC               
TthHB8I      1061  TCGA                 T^CGA
             1278  TCGA                
XmaCI         926  CCCGGG               C^CCGGG
             1220  CCCGGG              
XmaI          926  CCCGGG               C^CCGGG
             1220  CCCGGG              
XmnI          274  GAATTGATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              609  GAACTACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   65 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AvaII       
BalI        BbsI        BbvI        BcnI        BpmI        
BpuAI       BsaJI       BseRI       BsiYI       BslI        
BsmAI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       EaeI        EcoRII      EcoT14I     Fnu4HI      
FokI        HaeIII      HapII       HhaI        HinP1I      
HinfI       HpaII       ItaI        MboI        MboII       
MflI        MluNI       MnlI        MscI        MspA1I      
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       NspI        PleI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SmlI        
StyI        TseI        Tsp509I     TspRI       XhoII       
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