Restriction map for pF1KB9104
 (Linear) (Six-base) MAPSORT of: KIBB9104.seq  Check: 5152  from: 1  to: 1623



KIBB9104.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AatII G_ACGT'C

Cuts at:      0    1199    1623 
   Size:       1199     424
 
AclI AA'CG_TT

Cuts at:      0     427    1623 
   Size:        427    1196
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1084    1623 
   Size:       1084     539
 
AflIII A'CryG_T

Cuts at:      0    1252    1623 
   Size:       1252     371
 
AleI CACnn'nnGTG

Cuts at:      0    1108    1623 
   Size:       1108     515
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1126    1623 
   Size:       1126     497
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     331     363    1623 
   Size:        331      32    1260

  Fragments arranged by size: 

                1260     331      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     828    1623 
   Size:        828     795
 
AvaI C'yCGr_G

Cuts at:      0     683    1623 
   Size:        683     940
 
BamHI G'GATC_C

Cuts at:      0      25    1086    1623 
   Size:         25    1061     537

  Fragments arranged by size: 

                1061     537      25
 
BanI G'GyrC_C

Cuts at:      0    1102    1110    1602    1623 
   Size:       1102       8     492      21

  Fragments arranged by size: 

                1102     492      21       8
 
BanII G_rGCy'C

Cuts at:      0     260     575     632     812    1041    1562    1595    1623 
   Size:        260     315      57     180     229     521      33      28

  Fragments arranged by size: 

                 521     315     260     229     180      57      33      28
            
 
BglI GCCn_nnn'nGGC

Cuts at:      0      46     238    1623 
   Size:         46     192    1385

  Fragments arranged by size: 

                1385     192      46
 
BlpI GC'TnA_GC

Cuts at:      0     212     488    1623 
   Size:        212     276    1135

  Fragments arranged by size: 

                1135     276     212
 
Bme1580I G_kGCm'C

Cuts at:      0    1115    1508    1623 
   Size:       1115     393     115

  Fragments arranged by size: 

                1115     393     115
 
BmrI ACTGGGnnnn_n'

Cuts at:      0      29    1590    1623 
   Size:         29    1561      33

  Fragments arranged by size: 

                1561      33      29
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     709    1623 
   Size:        709     914
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     166     190     235     298     727     850     886 
   Size:        151      15      24      45      63     429     123      36

Cuts at:    886    1237    1438    1546    1561    1623 
   Size:        351     201     108      15      62

  Fragments arranged by size: 

                 429     351     201     151     123     108      63      62
                  45      36      24      15      15
 
Bpu10I CC'TnA_GC

Cuts at:      0    1040    1623 
   Size:       1040     583
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     589    1623 
   Size:        589    1034
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1381    1623 
   Size:       1381     242
 
BsaAI yAC'GTr

Cuts at:      0    1255    1359    1623 
   Size:       1255     104     264

  Fragments arranged by size: 

                1255     264     104
 
BsaBI GATnn'nnATC

Cuts at:      0    1091    1623 
   Size:       1091     532
 
BsaHI Gr'CG_yC

Cuts at:      0    1196    1623 
   Size:       1196     427
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     148     847    1000    1489    1623 
   Size:        148     699     153     489     134

  Fragments arranged by size: 

                 699     489     153     148     134
 
BseYI C'CCAG_C

Cuts at:      0     325     616     805     817    1204    1285    1613    1623 
   Size:        325     291     189      12     387      81     328      10

  Fragments arranged by size: 

                 387     328     325     291     189      81      12      10
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     199    1623 
   Size:        199    1424
 
BsiEI CG_ry'CG

Cuts at:      0    1132    1343    1623 
   Size:       1132     211     280

  Fragments arranged by size: 

                1132     280     211
 
BsiHKAI G_wGCw'C

Cuts at:      0     575    1562    1623 
   Size:        575     987      61

  Fragments arranged by size: 

                 987     575      61
 
BsmI GAATG_Cn'

Cuts at:      0     464     954    1623 
   Size:        464     490     669

  Fragments arranged by size: 

                 669     490     464
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     130     274    1455    1623 
   Size:        130     144    1181     168

  Fragments arranged by size: 

                1181     168     144     130
 
Bsp1286I G_dGCh'C

Cuts at:      0     260     575     632     812    1041    1115    1508    1562 
   Size:        260     315      57     180     229      74     393      54

Cuts at:   1562    1595    1623 
   Size:         33      28

  Fragments arranged by size: 

                 393     315     260     229     180      74      57      54
                  33      28
 
BspHI T'CATG_A

Cuts at:      0     966    1623 
   Size:        966     657
 
BsrBI CCG'CTC

Cuts at:      0    1146    1623 
   Size:       1146     477
 
BsrDI GCAATG_nn'

Cuts at:      0     588    1623 
   Size:        588    1035
 
BssHII G'CGCG_C

Cuts at:      0     460    1623 
   Size:        460    1163
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     498    1623 
   Size:        498    1125
 
BstYI r'GATC_y

Cuts at:      0      25     103     185     608    1086    1623 
   Size:         25      78      82     423     478     537

  Fragments arranged by size: 

                 537     478     423      82      78      25
 
BtgI C'CryG_G

Cuts at:      0      41    1106    1176    1571    1623 
   Size:         41    1065      70     395      52

  Fragments arranged by size: 

                1065     395      70      52      41
 
BtsI GCAGTG_nn'

Cuts at:      0     555     562     600     807    1623 
   Size:        555       7      38     207     816

  Fragments arranged by size: 

                 816     555     207      38       7
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     570     605    1623 
   Size:        570      35    1018

  Fragments arranged by size: 

                1018     570      35
 
EaeI y'GGCC_r

Cuts at:      0     110    1129    1340    1623 
   Size:        110    1019     211     283

  Fragments arranged by size: 

                1019     283     211     110
 
EagI C'GGCC_G

Cuts at:      0    1129    1340    1623 
   Size:       1129     211     283

  Fragments arranged by size: 

                1129     283     211
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0    1000    1468    1623 
   Size:       1000     468     155

  Fragments arranged by size: 

                1000     468     155
 
EcoICRI GAG'CTC

Cuts at:      0     573    1560    1623 
   Size:        573     987      63

  Fragments arranged by size: 

                 987     573      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     151     166     190     235     298     727     850     886 
   Size:        151      15      24      45      63     429     123      36

Cuts at:    886    1084    1237    1438    1546    1561    1623 
   Size:        198     153     201     108      15      62

  Fragments arranged by size: 

                 429     201     198     153     151     123     108      63
                  62      45      36      24      15      15
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     112    1623 
   Size:        112    1511
 
EcoO109I rG'GnC_Cy

Cuts at:      0     195     861     887    1374    1547    1623 
   Size:        195     666      26     487     173      76

  Fragments arranged by size: 

                 666     487     195     173      76      26
 
FspI TGC'GCA

Cuts at:      0     382     580    1623 
   Size:        382     198    1043

  Fragments arranged by size: 

                1043     382     198
 
FspAI rTGC'GCAy

Cuts at:      0     382    1623 
   Size:        382    1241
 
HaeII r_GCGC'y

Cuts at:      0    1212    1623 
   Size:       1212     411
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1023    1055    1623 
   Size:       1023      32     568

  Fragments arranged by size: 

                1023     568      32
 
HincII GTy'rAC

Cuts at:      0    1311    1623 
   Size:       1311     312
 
MscI TGG'CCA

Cuts at:      0     112    1623 
   Size:        112    1511
 
MslI CAynn'nnrTG

Cuts at:      0     419     509    1108    1623 
   Size:        419      90     599     515

  Fragments arranged by size: 

                 599     515     419      90
 
MspA1I CmG'CkG

Cuts at:      0     207     825    1494    1613    1623 
   Size:        207     618     669     119      10

  Fragments arranged by size: 

                 669     618     207     119      10
 
NcoI C'CATG_G

Cuts at:      0    1571    1623 
   Size:       1571      52
 
NspI r_CATG'y

Cuts at:      0     508     785    1002    1623 
   Size:        508     277     217     621

  Fragments arranged by size: 

                 621     508     277     217
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     773     828    1623 
   Size:        773      55     795

  Fragments arranged by size: 

                 795     773      55
 
PfoI T'CCnGG_A

Cuts at:      0      11     251     275    1623 
   Size:         11     240      24    1348

  Fragments arranged by size: 

                1348     240      24      11
 
PpuMI rG'GwC_Cy

Cuts at:      0     195     861     887    1547    1623 
   Size:        195     666      26     660      76

  Fragments arranged by size: 

                 666     660     195      76      26
 
PstI C_TGCA'G

Cuts at:      0      54     569    1623 
   Size:         54     515    1054

  Fragments arranged by size: 

                1054     515      54
 
PvuII CAG'CTG

Cuts at:      0     207     825    1613    1623 
   Size:        207     618     788      10

  Fragments arranged by size: 

                 788     618     207      10
 
SacI G_AGCT'C

Cuts at:      0     575    1562    1623 
   Size:        575     987      61

  Fragments arranged by size: 

                 987     575      61
 
SanDI GG'GwC_CC

Cuts at:      0     887    1623 
   Size:        887     736
 
SfcI C'TryA_G

Cuts at:      0      50     565    1623 
   Size:         50     515    1058

  Fragments arranged by size: 

                1058     515      50
 
SmlI C'TyrA_G

Cuts at:      0     604    1623 
   Size:        604    1019
 
SphI G_CATG'C

Cuts at:      0     785    1002    1623 
   Size:        785     217     621

  Fragments arranged by size: 

                 785     621     217
 
StuI AGG'CCT

Cuts at:      0     497     716    1623 
   Size:        497     219     907

  Fragments arranged by size: 

                 907     497     219
 
StyI C'CwwG_G

Cuts at:      0     113    1571    1623 
   Size:        113    1458      52

  Fragments arranged by size: 

                1458     113      52
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     409     499    1590    1605    1623 
   Size:        409      90    1091      15      18

  Fragments arranged by size: 

                1091     409      90      18      15
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     253     285    1623 
   Size:        253      32    1338

  Fragments arranged by size: 

                1338     253      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     333     825    1272    1623 
   Size:        333     492     447     351

  Fragments arranged by size: 

                 492     447     351     333
 
ZraI GAC'GTC

Cuts at:      0    1197    1623 
   Size:       1197     426
 

 Enzymes that do cut:

    AatII     AclI     AcuI   AflIII     AleI     AlfI     AloI    AlwNI
     AvaI    BamHI     BanI    BanII     BglI     BlpI Bme1580I     BmrI
     BplI     BpmI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I
    BspHI    BsrBI    BsrDI   BssHII   BstAPI    BstYI     BtgI     BtsI
    CspCI     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
     FspI    FspAI    HaeII    Hin4I   HincII     MscI     MslI   MspA1I
     NcoI     NspI    PflMI     PfoI    PpuMI     PstI    PvuII     SacI
    SanDI     SfcI     SmlI     SphI     StuI     StyI    TaqII     TstI
     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AfeI    AflII     AgeI     AhdI     ApaI
    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI     BbeI
     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII    BmgBI
     BmtI    BsaWI    BsaXI    BsiWI    BspEI    BspMI    BsrFI    BsrGI
    BssSI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     ClaI
     DraI   DraIII     DrdI     EarI    EcoRI    EcoRV     FalI     FseI
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI
     PasI     PciI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII    SacII     SalI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SrfI     SspI     SwaI     TatI  Tth111I     XbaI     XhoI
     XmaI     XmnI
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