Restriction map for pF1KB7022
##################################
# NO CUT ENZYMES (   86 enzymes) #
##################################

AccIII      AclI        AcsI        AflII       AflIII      
AgeI        Alw44I      AlwI        Aor51HI     ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
AviII       AvrII       BamHI       BbeI        BbrPI       
BclI        BfrI        BlnI        BsaAI       BsaWI       
BseAI       BsiEI       BsiWI       BsmI        BspDI       
BspEI       BspLU11I    BssHII      Bst1107I    BstBI       
BstZ17I     ClaI        CpoI        DraI        DrdI        
EagI        EclXI       Eco47III    Eco52I      EcoRI       
EcoT22I     FbaI        FspI        KasI        KspI        
MfeI        MluI        MroI        MunI        NarI        
NotI        NruI        NsiI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PvuI        RsrII       SacII       
SapI        SbfI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspI        SwaI        XbaI        XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AatII        2509  GACGTC               GACGT^C
Acc65I       1268  GGTACC               G^GTACC
AccII        2342  CGCG                 CG^CG
AlwNI        1405  CAGAGCCTG            CAG[ACGT][ACGT][ACGT]^CTG
AocI         1363  CCTGAGG              CC^T[ACGT]AGG
Asp718I      1268  GGTACC               G^GTACC
AspI         2485  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbsI          703  GTCTTC               GAAGAC(2/6)
BcgI          406  GCAGGCGTCTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        2614  GGATAC               GTATCC(6/5)
BlpI          671  GCTCAGC              GC^T[ACGT]AGC
Bpu1102I      671  GCTCAGC              GC^T[ACGT]AGC
BpuAI         703  GTCTTC               GAAGAC(2/6)
BsaBI         558  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI         411  CGTCTC               CGTCTC(1/5)
Bsp1407I     1610  TGTACA               T^GTACA
BsrBI        2758  GAGCGG               CCGCTC(-3/-3)
BsrGI        1610  TGTACA               T^GTACA
BssSI        1927  CACGAG               CACGAG(-5/-1)
BstAPI       1404  GCAGAGCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI        2342  CGCG                 CG^CG
BstXI        1156  CCAGGAGGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I       1363  CCTGAGG              CC^T[ACGT]AGG
CelII         671  GCTCAGC              GC^T[ACGT]AGC
DraIII       1390  CACAAGGTG            CAC[ACGT][ACGT][ACGT]^GTG
EarI         1540  CTCTTC               CTCTTC(1/4)
Eco81I       1363  CCTGAGG              CC^T[ACGT]AGG
EcoRV        2872  GATATC               GAT^ATC
FseI          433  GGCCGGCC             GGCCGG^CC
HaeII         280  AGCGCC               [GA]GCGC^[CT]
HindIII      1048  AAGCTT               A^AGCTT
HpaI          691  GTTAAC               GTT^AAC
KpnI         1268  GGTACC               GGTAC^C
Ksp632I      1540  CTCTTC               CTCTTC(1/4)
MamI          558  GATGTCCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI         2342  CGCG                 CG^CG
NcoI         1962  CCATGG               C^CATGG
NdeI         1218  CATATG               CA^TATG
NheI          330  GCTAGC               G^CTAGC
PaeR7I       2110  CTCGAG               C^TCGAG
PflFI        2485  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PvuII        1702  CAGCTG               CAG^CTG
SalI         2089  GTCGAC               G^TCGAC
ScaI         1991  AGTACT               AGT^ACT
SexAI        1637  ACCAGGT              A^CC[AT]GGT
SfiI         2670  GGCCTGCTGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI        1610  TGTACA               T^GTACA
Tsp509I      2317  AATT                 ^AATT
Tth111I      2485  GACTGTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XhoI         2110  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   28 enzymes) #
###################################

AccI         2089  GTCGAC               GT^[AC][GT]AC
             2491  GTCTAC              
AhdI         2358  GACCAATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2509  GACGTCTGGTC         
AspEI        2358  GACCAATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2509  GACGTCTGGTC         
AvaI         2077  CCCGAG               C^[CT]CG[GA]G
             2110  CTCGAG              
BbvCI         204  CCTCAGC              CCTCAGC(-5/-2)
             2035  GCTGAGG             
BglI         1913  GCCACCTGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2671  GCCTGCTGGGC         
BglII        2540  AGATCT               A^GATCT
             2714  AGATCT              
BsaI          263  GAGACC               GGTCTC(1/5)
             1423  GAGACC              
BsoBI        2077  CCCGAG               C^[CT]CG[GA]G
             2110  CTCGAG              
BspHI        1907  TCATGA               T^CATGA
             2408  TCATGA              
BsrDI         131  GCAATG               GCAATG(2/0)
             2438  GCAATG              
BsrFI         434  GCCGGC               [GA]^CCGG[CT]
              817  GCCGGC              
BstEII        111  GGTGACC              G^GT[ACGT]ACC
              936  GGTGACC             
BstPI         111  GGTGACC              G^GT[ACGT]ACC
              936  GGTGACC             
BstYI        2540  AGATCT               [GA]^GATC[CT]
             2714  AGATCT              
Cfr10I        434  GCCGGC               [GA]^CCGG[CT]
              817  GCCGGC              
Eam1105I     2358  GACCAATGGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             2509  GACGTCTGGTC         
EcoO65I       111  GGTGACC              G^GT[ACGT]ACC
              936  GGTGACC             
MflI         2540  AGATCT               [GA]^GATC[CT]
             2714  AGATCT              
MseI          692  TTAA                 T^TAA
             1001  TTAA                
NaeI          434  GCCGGC               GCC^GGC
              817  GCCGGC              
NgoMIV        434  GCCGGC               G^CCGGC
              817  GCCGGC              
NspI          813  ACATGC               [GA]CATG^[CT]
             1565  GCATGT              
PleI         2203  GACTC                GAGTC(4/5)
             2416  GACTC               
RcaI         1907  TCATGA               T^CATGA
             2408  TCATGA              
SmlI          906  CTTGAG               C^T[CT][GA]AG
             2110  CTCGAG              
Tru9I         692  TTAA                 T^TAA
             1001  TTAA                
XhoII        2540  AGATCT               [GA]^GATC[CT]
             2714  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   94 enzymes) #
#########################################

AciI        AcyI        AfaI        AluI        ApaI        
AspHI       AvaII       BalI        BanI        BanII       
BbvI        BcnI        BfaI        BmyI        BpmI        
BsaHI       BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       DsaI        EaeI        EcoNI       EcoO109I    
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HinfI       HpaII       
HphI        ItaI        MaeI        MaeII       MaeIII      
MboI        MboII       MluNI       MnlI        MscI        
MslI        MspA1I      MspI        MvaI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PpuMI       PstI        RsaI        SacI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        StuI        
StyI        TaqI        TfiI        TseI        Tsp45I      
TspRI       TthHB8I     Van91I      XcmI        
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