Restriction map for pF1KB3289
 (Linear) (Six-base) MAPSORT of: KIBB3289.seq  Check: 1313  from: 1  to: 2409



KIBB3289.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     765    1098    1117    1182    1369    1518    1602 
   Size:        449     316     333      19      65     187     149      84

Cuts at:   1602    1705    1854    2106    2190    2274    2409 
   Size:        103     149     252      84      84     135

  Fragments arranged by size: 

                 449     333     316     252     187     149     149     135
                 103      84      84      84      65      19
 
AflII C'TTAA_G

Cuts at:      0     919    2409 
   Size:        919    1490
 
AflIII A'CryG_T

Cuts at:      0     841     903    2409 
   Size:        841      62    1506

  Fragments arranged by size: 

                1506     841      62
 
AleI CACnn'nnGTG

Cuts at:      0     656    2409 
   Size:        656    1753
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     335    2409 
   Size:        335    2074
 
ApaI G_GGCC'C

Cuts at:      0      79    2409 
   Size:         79    2330
 
ApaLI G'TGCA_C

Cuts at:      0    1964    2074    2409 
   Size:       1964     110     335

  Fragments arranged by size: 

                1964     335     110
 
ApoI r'AATT_y

Cuts at:      0     228     422     566     943    1206    1542    1808    2409 
   Size:        228     194     144     377     263     336     266     601

  Fragments arranged by size: 

                 601     377     336     266     263     228     194     144
            
 
BamHI G'GATC_C

Cuts at:      0    1820    2409 
   Size:       1820     589
 
BanII G_rGCy'C

Cuts at:      0      79    1714    2409 
   Size:         79    1635     695

  Fragments arranged by size: 

                1635     695      79
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     225    1375    1860    2131    2196    2207    2409 
   Size:        225    1150     485     271      65      11     202

  Fragments arranged by size: 

                1150     485     271     225     202      65      11
 
BclI T'GATC_A

Cuts at:      0     353     717    2409 
   Size:        353     364    1692

  Fragments arranged by size: 

                1692     364     353
 
BfrBI ATG'CAT

Cuts at:      0    1183    2064    2409 
   Size:       1183     881     345

  Fragments arranged by size: 

                1183     881     345
 
BglII A'GATC_T

Cuts at:      0    1800    2409 
   Size:       1800     609
 
Bme1580I G_kGCm'C

Cuts at:      0      79    1968    2078    2409 
   Size:         79    1889     110     331

  Fragments arranged by size: 

                1889     331     110      79
 
BmgBI CAC'GTC

Cuts at:      0      36    2409 
   Size:         36    2373
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     485     871    1166    2258    2409 
   Size:        485     386     295    1092     151

  Fragments arranged by size: 

                1092     485     386     295     151
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     777    1430    1514    2018    2409 
   Size:        777     653      84     504     391

  Fragments arranged by size: 

                 777     653     504     391      84
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     519    2118    2409 
   Size:        519    1599     291

  Fragments arranged by size: 

                1599     519     291
 
BsaAI yAC'GTr

Cuts at:      0     987    2409 
   Size:        987    1422
 
BsaBI GATnn'nnATC

Cuts at:      0     806    2409 
   Size:        806    1603
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      70    1111    1279    1699    1867    2409 
   Size:         70    1041     168     420     168     542

  Fragments arranged by size: 

                1041     542     420     168     168      70
 
BseYI C'CCAG_C

Cuts at:      0      72    2409 
   Size:         72    2337
 
BsiEI CG_ry'CG

Cuts at:      0    1959    2409 
   Size:       1959     450
 
BsiHKAI G_wGCw'C

Cuts at:      0    1714    1968    2078    2409 
   Size:       1714     254     110     331

  Fragments arranged by size: 

                1714     331     254     110
 
Bsp1286I G_dGCh'C

Cuts at:      0      79    1714    1968    2078    2409 
   Size:         79    1635     254     110     331

  Fragments arranged by size: 

                1635     331     254     110      79
 
BspHI T'CATG_A

Cuts at:      0     633    2409 
   Size:        633    1776
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     137    2409 
   Size:        137    2272
 
BsrDI GCAATG_nn'

Cuts at:      0    1021    1056    1521    2409 
   Size:       1021      35     465     888

  Fragments arranged by size: 

                1021     888     465      35
 
BstYI r'GATC_y

Cuts at:      0    1800    1820    2409 
   Size:       1800      20     589

  Fragments arranged by size: 

                1800     589      20
 
BtsI GCAGTG_nn'

Cuts at:      0      26    2409 
   Size:         26    2383
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0    1503    1538    2409 
   Size:       1503      35     871

  Fragments arranged by size: 

                1503     871      35
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     411    2409 
   Size:        411    1998
 
EarI CTCTTCn'nnn_

Cuts at:      0     281    1522    1763    1942    2409 
   Size:        281    1241     241     179     467

  Fragments arranged by size: 

                1241     467     281     241     179
 
EcoICRI GAG'CTC

Cuts at:      0    1712    2409 
   Size:       1712     697
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     765     777    1098    1117    1182    1369    1430 
   Size:        449     316      12     321      19      65     187      61

Cuts at:   1430    1514    1518    1602    1705    1854    2018    2106    2190 
   Size:         84       4      84     103     149     164      88      84

Cuts at:   2190    2274    2409 
   Size:         84     135

  Fragments arranged by size: 

                 449     321     316     187     164     149     135     103
                  88      84      84      84      84      65      61      19
                  12       4
 
EcoO109I rG'GnC_Cy

Cuts at:      0      76    1109    2409 
   Size:         76    1033    1300

  Fragments arranged by size: 

                1300    1033      76
 
EcoRI G'AATT_C

Cuts at:      0     943    1206    1542    2409 
   Size:        943     263     336     867

  Fragments arranged by size: 

                 943     867     336     263
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     307    2064    2409 
   Size:        307    1757     345

  Fragments arranged by size: 

                1757     345     307
 
HaeII r_GCGC'y

Cuts at:      0     959    2409 
   Size:        959    1450
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     262     294     615     647     702     734    1185    1217 
   Size:        262      32     321      32      55      32     451      32

Cuts at:   1217    1449    1481    1941    1953    1973    1985    2409 
   Size:        232      32     460      12      20      12     424

  Fragments arranged by size: 

                 460     451     424     321     262     232      55      32
                  32      32      32      32      20      12      12
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     601    1183    1939    2409 
   Size:        601     582     756     470

  Fragments arranged by size: 

                 756     601     582     470
 
MslI CAynn'nnrTG

Cuts at:      0     635     656    1005    1173    1257    1425    1509    1745 
   Size:        635      21     349     168      84     168      84     236

Cuts at:   1745    1761    1929    2013    2097    2153    2181    2333    2409 
   Size:         16     168      84      84      56      28     152      76

  Fragments arranged by size: 

                 635     349     236     168     168     168     152      84
                  84      84      84      76      56      28      21      16
            
 
MspA1I CmG'CkG

Cuts at:      0    1734    2409 
   Size:       1734     675
 
NdeI CA'TA_TG

Cuts at:      0    1426    2409 
   Size:       1426     983
 
NsiI A_TGCA'T

Cuts at:      0    1185    2066    2409 
   Size:       1185     881     343

  Fragments arranged by size: 

                1185     881     343
 
NspI r_CATG'y

Cuts at:      0     845     907    1187    2064    2409 
   Size:        845      62     280     877     345

  Fragments arranged by size: 

                 877     845     345     280      62
 
PciI A'CATG_T

Cuts at:      0     841     903    2409 
   Size:        841      62    1506

  Fragments arranged by size: 

                1506     841      62
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     620    2409 
   Size:        620    1789
 
PmlI CAC'GTG

Cuts at:      0     987    2409 
   Size:        987    1422
 
PsiI TTA'TAA

Cuts at:      0    1088    2264    2409 
   Size:       1088    1176     145

  Fragments arranged by size: 

                1176    1088     145
 
PspOMI G'GGCC_C

Cuts at:      0      75    2409 
   Size:         75    2334
 
PvuII CAG'CTG

Cuts at:      0    1734    2409 
   Size:       1734     675
 
SacI G_AGCT'C

Cuts at:      0    1714    2409 
   Size:       1714     695
 
SapI GCTCTTCn'nnn_

Cuts at:      0     281    1522    2409 
   Size:        281    1241     887

  Fragments arranged by size: 

                1241     887     281
 
ScaI AGT'ACT

Cuts at:      0    2119    2409 
   Size:       2119     290
 
SexAI A'CCwGG_T

Cuts at:      0     334     395    2409 
   Size:        334      61    2014

  Fragments arranged by size: 

                2014     334      61
 
SfcI C'TryA_G

Cuts at:      0     173     964    1815    2224    2409 
   Size:        173     791     851     409     185

  Fragments arranged by size: 

                 851     791     409     185     173
 
SmlI C'TyrA_G

Cuts at:      0     498     919    2133    2409 
   Size:        498     421    1214     276

  Fragments arranged by size: 

                1214     498     421     276
 
StuI AGG'CCT

Cuts at:      0    1123    1879    2131    2409 
   Size:       1123     756     252     278

  Fragments arranged by size: 

                1123     756     278     252
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1945    2201    2409 
   Size:       1945     256     208

  Fragments arranged by size: 

                1945     256     208
 
TatI w'GTAC_w

Cuts at:      0     648     900    1429    2117    2409 
   Size:        648     252     529     688     292

  Fragments arranged by size: 

                 688     648     529     292     252
 
Tth111I GACn'n_nGTC

Cuts at:      0     537    2409 
   Size:        537    1872
 
XmnI GAAnn'nnTTC

Cuts at:      0       7     526    1027    1168    1531    1615    1867    1951 
   Size:          7     519     501     141     363      84     252      84

Cuts at:   1951    2035    2409 
   Size:         84     374

  Fragments arranged by size: 

                 519     501     374     363     252     141      84      84
                  84       7
 

 Enzymes that do cut:

     AcuI    AflII   AflIII     AleI     AlfI     ApaI    ApaLI     ApoI
    BamHI    BanII     BbsI     BclI    BfrBI    BglII Bme1580I    BmgBI
     BmrI     BpmI    BpuEI    BsaAI    BsaBI    BseRI    BseYI    BsiEI
  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BstYI     BtsI    CspCI
     DrdI     EarI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI    HaeII
    Hin4I     MmeI     MslI   MspA1I     NdeI     NsiI     NspI     PciI
    PflMI     PmlI     PsiI   PspOMI    PvuII     SacI     SapI     ScaI
    SexAI     SfcI     SmlI     StuI    TaqII     TatI  Tth111I     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI     AgeI     AhdI
     AloI    AlwNI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI
     BanI     BbeI    BbvCI     BcgI    BciVI     BglI     BlpI     BmtI
     BplI   Bpu10I     BsaI    BsaHI    BsaWI    BsaXI     BsgI    BsiWI
     BsmI    BsmBI    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI
     ClaI     DraI   DraIII     EaeI     EagI     EciI    EcoNI    EcoRV
     FseI     FspI    FspAI   HincII  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI   NgoMIV     NheI
     NotI     NruI     PacI     PasI     PfoI     PmeI     PpiI    PpuMI
    PshAI    PspXI     PsrI     PstI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI
     SpeI     SphI     SrfI     SspI     StyI     SwaI     TstI     XbaI
     XcmI     XhoI     XmaI     ZraI
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