Restriction map for pF1KB3641
 (Circular) (Six-base) MAPSORT of: pF1KB3641.seq  Check: 1763  from: 1  to: 4055



pF1KB3641  4055 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:    566     968    1093     566 
   Size:        402     125    3528

  Fragments arranged by size: 

                3528     402     125
 
Acc65I G'GTAC_C

Cuts at:   1071    1071 
   Size:       4055
 
AclI AA'CG_TT

Cuts at:   3895    3895 
   Size:       4055
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    317    1880    2312    2665    3327     317 
   Size:       1563     432     353     662    1045

  Fragments arranged by size: 

                1563    1045     662     432     353
 
AflIII A'CryG_T

Cuts at:   1407    3207    3488    1407 
   Size:       1800     281    1974

  Fragments arranged by size: 

                1974    1800     281
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:    141     141 
   Size:       4055
 
AlwNI CAG_nnn'CTG

Cuts at:    223     738    1360    2798     223 
   Size:        515     622    1438    1480

  Fragments arranged by size: 

                1480    1438     622     515
 
ApaLI G'TGCA_C

Cuts at:   2893    2893 
   Size:       4055
 
ApoI r'AATT_y

Cuts at:    922     937    1003    1059    3408    3664     922 
   Size:         15      66      56    2349     256    1313

  Fragments arranged by size: 

                2349    1313     256      66      56      15
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4055
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4055
 
AvaI C'yCGr_G

Cuts at:   1075    1075 
   Size:       4055
 
AvrII C'CTAG_G

Cuts at:    138     138 
   Size:       4055
 
BamHI G'GATC_C

Cuts at:   1080    1080 
   Size:       4055
 
BanI G'GyrC_C

Cuts at:   1071    1485    1733    1768    1071 
   Size:        414     248      35    3358

  Fragments arranged by size: 

                3358     414     248      35
 
BanII G_rGCy'C

Cuts at:   1069    2099    1069 
   Size:       1030    3025
 
BbeI G_GCGC'C

Cuts at:   1489    1737    1489 
   Size:        248    3807
 
BciVI GTATCCnnnnn_n'

Cuts at:   1953    3009    4055    1953 
   Size:       1056    1046    1953

  Fragments arranged by size: 

                1953    1056    1046
 
BclI T'GATC_A

Cuts at:   1575    1575 
   Size:       4055
 
BglI GCCn_nnn'nGGC

Cuts at:    140     140 
   Size:       4055
 
BglII A'GATC_T

Cuts at:   1570    3607    1570 
   Size:       2037    2018
 
BlpI GC'TnA_GC

Cuts at:   1167    1167 
   Size:       4055
 
Bme1580I G_kGCm'C

Cuts at:   1680    1773    2897    1680 
   Size:         93    1124    2838

  Fragments arranged by size: 

                2838    1124      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1267    1348    1683    3827    1267 
   Size:         81     335    2144    1495

  Fragments arranged by size: 

                2144    1495     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2422    2422 
   Size:       4055
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    533     533 
   Size:       4055
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1231    2110    2824    3122    1231 
   Size:        879     714     298    2164

  Fragments arranged by size: 

                2164     879     714     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    316    3759     316 
   Size:       3443     612
 
BsaAI yAC'GTr

Cuts at:   1408    1408 
   Size:       4055
 
BsaBI GATnn'nnATC

Cuts at:      6    1569    3606       6 
   Size:       1563    2037     455

  Fragments arranged by size: 

                2037    1563     455
 
BsaHI Gr'CG_yC

Cuts at:   1486    1734    2436    3932    1486 
   Size:        248     702    1496    1609

  Fragments arranged by size: 

                1609    1496     702     248
 
BsaWI w'CCGG_w

Cuts at:   1244    1765    2443    2854    3001    3275    1244 
   Size:        521     678     411     147     274    2024

  Fragments arranged by size: 

                2024     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    709     709 
   Size:       4055
 
BseYI C'CCAG_C

Cuts at:   1480    2238    2903    3311    1480 
   Size:        758     665     408    2224

  Fragments arranged by size: 

                2224     758     665     408
 
BsiEI CG_ry'CG

Cuts at:    104    1597    1643    2873     104 
   Size:       1493      46    1230    1286

  Fragments arranged by size: 

                1493    1286    1230      46
 
BsiHKAI G_wGCw'C

Cuts at:   1069    1847    2037    2897    1069 
   Size:        778     190     860    2227

  Fragments arranged by size: 

                2227     860     778     190
 
BsmI GAATG_Cn'

Cuts at:    288     876     288 
   Size:        588    3467
 
Bsp1286I G_dGCh'C

Cuts at:   1069    1680    1773    1847    2037    2099    2897    1069 
   Size:        611      93      74     190      62     798    2227

  Fragments arranged by size: 

                2227     798     611     190      93      74      62
 
BspHI T'CATG_A

Cuts at:   1017    2487    1017 
   Size:       1470    2585
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    533    1105    1319    1621    2002     533 
   Size:        572     214     302     381    2586

  Fragments arranged by size: 

                2586     572     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    2347    2401    3885    4050      50 
   Size:       2297      54    1484     165      55

  Fragments arranged by size: 

                2297    1484     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:    876    1967     876 
   Size:       1091    2964
 
BsrFI r'CCGG_y

Cuts at:   2053    2234    2053 
   Size:        181    3874
 
BssSI C'ACGA_G

Cuts at:   2326    3034    2326 
   Size:        708    3347
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1432    1432 
   Size:       4055
 
BstBI TT'CG_AA

Cuts at:   2416    2416 
   Size:       4055
 
BstYI r'GATC_y

Cuts at:      1    1080    1547    1570    1905    2151    2457    2469    2555 
   Size:       1079     467      23     335     246     306      12      86

Cuts at:   2555    3607       1 
   Size:       1052     449

  Fragments arranged by size: 

                1079    1052     467     449     335     306     246      86
                  23      12
 
BtgI C'CryG_G

Cuts at:    518     765     518 
   Size:        247    3808
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2005    2186    2349    2005 
   Size:        181     163    3711

  Fragments arranged by size: 

                3711     181     163
 
BtsI GCAGTG_nn'

Cuts at:    125    1298     125 
   Size:       1173    2882
 
DraI TTT'AAA

Cuts at:    315    1054     315 
   Size:        739    3316
 
DrdI GACnn_nn'nnGTC

Cuts at:   1761    3105    1761 
   Size:       1344    2711
 
EaeI y'GGCC_r

Cuts at:    227     579    1640    1814    2205    2232    3577     227 
   Size:        352    1061     174     391      27    1345     705

  Fragments arranged by size: 

                1345    1061     705     391     352     174      27
 
EagI C'GGCC_G

Cuts at:   1640    1640 
   Size:       4055
 
EarI CTCTTCn'nnn_

Cuts at:   2078    2288    2078 
   Size:        210    3845
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2263    3005    3151    3865    2263 
   Size:        742     146     714    2453

  Fragments arranged by size: 

                2453     742     714     146
 
EcoICRI GAG'CTC

Cuts at:   1067    1067 
   Size:       4055
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    317     533    1880    2312    2665    3327     317 
   Size:        216    1347     432     353     662    1045

  Fragments arranged by size: 

                1347    1045     662     432     353     216
 
EcoO109I rG'GnC_Cy

Cuts at:    735    1194     735 
   Size:        459    3596
 
EcoRI G'AATT_C

Cuts at:    922    1059     922 
   Size:        137    3918
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    343    2074     343 
   Size:       1731    2324
 
FspI TGC'GCA

Cuts at:   1836    1836 
   Size:       4055
 
HaeII r_GCGC'y

Cuts at:   1489    1737    2967    3738    1489 
   Size:        248    1230     771    1806

  Fragments arranged by size: 

                1806    1230     771     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    626     658     661     693     879     911    1018    1050    1897 
   Size:         32       3      32     186      32     107      32     847

Cuts at:   1897    1927    1929    1959     626 
   Size:         30       2      30    2722

  Fragments arranged by size: 

                2722     847     186     107      32      32      32      32
                  30      30       3       2
 
HincII GTy'rAC

Cuts at:   1094    1094 
   Size:       4055
 
KasI G'GCGC_C

Cuts at:   1485    1733    1485 
   Size:        248    3807
 
KpnI G_GTAC'C

Cuts at:   1075    1075 
   Size:       4055
 
MluI A'CGCG_T

Cuts at:   3488    3488 
   Size:       4055
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    365    2814    2998     365 
   Size:       2449     184    1422

  Fragments arranged by size: 

                2449    1422     184
 
MscI TGG'CCA

Cuts at:    229    1816     229 
   Size:       1587    2468
 
MslI CAynn'nnrTG

Cuts at:    362     485     881     932     947    2044    2171     362 
   Size:        123     396      51      15    1097     127    2246

  Fragments arranged by size: 

                2246    1097     396     127     123      51      15
 
MspA1I CmG'CkG

Cuts at:    795    1166    1480    1840    2624    2869     795 
   Size:        371     314     360     784     245    1981

  Fragments arranged by size: 

                1981     784     371     360     314     245
 
NaeI GCC'GGC

Cuts at:   2236    2236 
   Size:       4055
 
NarI GG'CG_CC

Cuts at:   1486    1734    1486 
   Size:        248    3807
 
NdeI CA'TA_TG

Cuts at:    573     573 
   Size:       4055
 
NgoMIV G'CCGG_C

Cuts at:   2234    2234 
   Size:       4055
 
NspI r_CATG'y

Cuts at:   1108    1608    3211    1108 
   Size:        500    1603    1952

  Fragments arranged by size: 

                1952    1603     500
 
PciI A'CATG_T

Cuts at:   3207    3207 
   Size:       4055
 
PmeI GTTT'AAAC

Cuts at:   1054    1054 
   Size:       4055
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2501    2533    2501 
   Size:         32    4023
 
PpuMI rG'GwC_Cy

Cuts at:    735     735 
   Size:       4055
 
PshAI GACnn'nnGTC

Cuts at:    609     609 
   Size:       4055
 
PstI C_TGCA'G

Cuts at:   1102    1787    1102 
   Size:        685    3370
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4055
 
PvuII CAG'CTG

Cuts at:    795    1480    1840     795 
   Size:        685     360    3010

  Fragments arranged by size: 

                3010     685     360
 
RsrII CG'GwC_CG

Cuts at:   2250    2250 
   Size:       4055
 
SacI G_AGCT'C

Cuts at:   1069    1069 
   Size:       4055
 
SalI G'TCGA_C

Cuts at:   1092    1092 
   Size:       4055
 
SapI GCTCTTCn'nnn_

Cuts at:   2078    2288    2078 
   Size:        210    3845
 
SbfI CC_TGCA'GG

Cuts at:   1102    1102 
   Size:       4055
 
ScaI AGT'ACT

Cuts at:   3572    3572 
   Size:       4055
 
SfcI C'TryA_G

Cuts at:     33     659    1098    1783    2751    2942      33 
   Size:        626     439     685     968     191    1146

  Fragments arranged by size: 

                1146     968     685     626     439     191
 
SfoI GGC'GCC

Cuts at:   1487    1735    1487 
   Size:        248    3807
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4055
 
SmaI CCC'GGG

Cuts at:   1077    1077 
   Size:       4055
 
SmlI C'TyrA_G

Cuts at:   1210    2125    2839    3101    1210 
   Size:        915     714     262    2164

  Fragments arranged by size: 

                2164     915     714     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4055
 
SphI G_CATG'C

Cuts at:   1108    1608    1108 
   Size:        500    3555
 
StyI C'CwwG_G

Cuts at:    138    1189    1543     138 
   Size:       1051     354    2650

  Fragments arranged by size: 

                2650    1051     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1638    1771    2230    2439    1638 
   Size:        133     459     209    3254

  Fragments arranged by size: 

                3254     459     209     133
 
TatI w'GTAC_w

Cuts at:   3570    3570 
   Size:       4055
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:    498     530     817     849    3572    3604     498 
   Size:         32     287      32    2723      32     949

  Fragments arranged by size: 

                2723     949     287      32      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1852    1852 
   Size:       4055
 
XbaI T'CTAG_A

Cuts at:   1086    1086 
   Size:       4055
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1550    1550 
   Size:       4055
 
XmaI C'CCGG_G

Cuts at:   1075    1075 
   Size:       4055
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AlfI    AlwNI    ApaLI
     ApoI     AseI    AsiSI     AvaI    AvrII    BamHI     BanI    BanII
     BbeI    BciVI     BclI     BglI    BglII     BlpI Bme1580I     BmrI
     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BssSI   BstAPI    BstBI    BstYI     BtgI
    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NdeI   NgoMIV     NspI     PciI     PmeI     PpiI
    PpuMI    PshAI     PstI     PvuI    PvuII    RsrII     SacI     SalI
     SapI     SbfI     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI
     SpeI     SphI     StyI    TaqII     TatI     TstI  Tth111I     XbaI
     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AhdI     AleI     AloI
     ApaI     AscI     BaeI     BbsI    BbvCI     BcgI    BfrBI    BmgBI
     BmtI   Bpu10I    BsaXI     BsgI    BsiWI    BsmBI    BspEI    BsrGI
   BssHII   BstEII    BstXI  BstZ17I   Bsu36I     ClaI    CspCI   DraIII
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NcoI
     NheI     NotI     NruI     NsiI     PacI     PasI    PflMI     PfoI
     PmlI     PsiI   PspOMI    PspXI     PsrI    SacII    SanDI    SexAI
     SfiI    SgrAI    SnaBI     SrfI     SspI     StuI     SwaI     XhoI
     XmnI     ZraI
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