Restriction map for pF1KB3264
##################################
# NO CUT ENZYMES (  113 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        AhdI        Alw44I      Aor51HI     
ApaI        ApaLI       AscI        Asp700I     Asp718I     
AspEI       AspI        AvrII       BanII       BbeI        
BbrPI       BbvCI       BclI        BfrI        BglI        
BlnI        BlpI        Bpu1102I    BsaAI       BsaBI       
BsaHI       BsaWI       BseAI       BsgI        BsiEI       
BsiWI       BsmI        Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssSI       
Bst1107I    BstZ17I     CelII       Cfr10I      ClaI        
CpoI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoRI       EcoRV       FauI        
FbaI        FseI        HaeII       HgaI        Hin1I       
HincII      HindII      HindIII     HpaI        KasI        
KpnI        KspI        MamI        MluI        MroI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NspI        PacI        PflFI       
PinAI       PmaCI       PmeI        PmlI        PshAI       
Psp1406I    PvuI        RsrII       SacI        SacII       
SalI        SapI        SbfI        SexAI       SfiI        
SfoI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SwaI        Tth111I     
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccI         1675  GTAGAC               GT^[AC][GT]AC
AflIII        987  ACGTGT               A^C[GA][CT]GT
AlwNI        1015  CAGGAACTG            CAG[ACGT][ACGT][ACGT]^CTG
AspHI        1649  GAGCAC               G[AT]GC[AT]^C
AvaI         1565  CTCGAG               C^[CT]CG[GA]G
AviII          99  TGCGCA               TGC^GCA
BalI          365  TGGCCA               TGG^CCA
BamHI        1770  GGATCC               G^GATCC
BanI          657  GGCACC               G^G[CT][GA]CC
BbsI          412  GAAGAC               GAAGAC(2/6)
BcgI         2372  GCATTGCCATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1866  GGATAC               GTATCC(6/5)
BpuAI         412  GAAGAC               GAAGAC(2/6)
BsiHKAI      1649  GAGCAC               G[AT]GC[AT]^C
BsmBI         250  GAGACG               CGTCTC(1/5)
BsoBI        1565  CTCGAG               C^[CT]CG[GA]G
BspHI         756  TCATGA               T^CATGA
BspMI        1791  GCAGGT               ACCTGC(4/8)
BssHII         20  GCGCGC               G^CGCGC
BstAPI       1580  GCAAAGTCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI        1220  TTCGAA               TT^CGAA
BstEII         66  GGTGACC              G^GT[ACGT]ACC
BstPI          66  GGTGACC              G^GT[ACGT]ACC
BstXI        2519  CCACATATGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII         61  CACCGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DrdI         2097  GACCAAAGAGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI            7  CCGTGG               C^C[GA][CT]GG
EaeI          365  TGGCCA               [CT]^GGCC[GA]
EcoO65I        66  GGTGACC              G^GT[ACGT]ACC
EcoT14I      1422  CCAAGG               C^C[AT][AT]GG
EcoT22I      1552  ATGCAT               ATGCA^T
FspI           99  TGCGCA               TGC^GCA
MfeI         1680  CAATTG               C^AATTG
MluNI         365  TGGCCA               TGG^CCA
MscI          365  TGGCCA               TGG^CCA
MunI         1680  CAATTG               C^AATTG
NdeI         2522  CATATG               CA^TATG
NsiI         1552  ATGCAT               ATGCA^T
NspV         1220  TTCGAA               TT^CGAA
PaeR7I       1565  CTCGAG               C^TCGAG
PflMI        1014  CCAGGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI        1839  AGGACCC              [GA]G^G[AT]CC[CT]
RcaI          756  TCATGA               T^CATGA
SfuI         1220  TTCGAA               TT^CGAA
SspI          647  AATATT               AAT^ATT
StuI          426  AGGCCT               AGG^CCT
StyI         1422  CCAAGG               C^C[AT][AT]GG
Van91I       1014  CCAGGAACTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI         1233  TCTAGA               T^CTAGA
XhoI         1565  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccII          21  CGCG                 CG^CG
              112  CGCG                
AciI          111  CCGC                 CCGC(-3/-1)
              190  CCGC                
AseI          403  ATTAAT               AT^TAAT
             2059  ATTAAT              
AsnI          403  ATTAAT               AT^TAAT
             2059  ATTAAT              
BglII         471  AGATCT               A^GATCT
             1335  AGATCT              
BmyI         1649  GAGCAC               G[AGT]GC[ACT]^C
             1981  GTGCCC              
BsaI         1126  GAGACC               GGTCTC(1/5)
             2047  GAGACC              
BseRI        1009  CTCCTC               GAGGAG(10/8)
             2483  GAGGAG              
Bsp1286I     1649  GAGCAC               G[AGT]GC[ACT]^C
             1981  GTGCCC              
BstUI          21  CGCG                 CG^CG
              112  CGCG                
DraII         379  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1839  AGGACCC             
EcoO109I      379  AGGCCCT              [GA]G^G[ACGT]CC[CT]
             1839  AGGACCC             
MaeII         253  ACGT                 A^CGT
              987  ACGT                
MslI         2454  CACAAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
             2520  CACATATGTG          
MspA1I        133  CAGCTG               C[AC]G^C[GT]G
             2315  CAGCTG              
MvnI           21  CGCG                 CG^CG
              112  CGCG                
PshBI         403  ATTAAT               AT^TAAT
             2059  ATTAAT              
PstI          843  CTGCAG               CTGCA^G
             2284  CTGCAG              
PvuII         133  CAGCTG               CAG^CTG
             2315  CAGCTG              
SmlI         1565  CTCGAG               C^T[CT][GA]AG
             2251  CTCAAG              
XcmI         1079  CCATGAAGGACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             1422  CCAAGGACAAAATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   74 enzymes) #
#########################################

AcsI        AfaI        AluI        AlwI        AocI        
ApoI        AvaII       BbvI        BcnI        BfaI        
BpmI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       BsrDI       BsrI        BssKI       BstF5I      
BstNI       BstYI       Bsu36I      Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        EarI        
Eco81I      EcoRII      Fnu4HI      FokI        HaeIII      
HapII       HhaI        HinP1I      HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeI        MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MspI        MvaI        MwoI        NciI        NdeII       
NlaIII      NlaIV       PleI        RsaI        Sau3AI      
Sau96I      ScaI        ScrFI       SfaNI       SfcI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII       
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