Restriction map for pF1KB5823
##################################
# NO CUT ENZYMES (  186 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        AlwNI       AocI        Aor51HI     
ApaI        AscI        AseI        AsnI        Asp718I     
AspEI       AspI        AvaI        AvaII       AviII       
AvrII       BalI        BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BbvI        BcgI        
BciVI       BcnI        BfrI        BglI        BglII       
BlnI        BlpI        Bpu1102I    BpuAI       BsaHI       
BsaI        BsaJI       BsaWI       BseAI       BseRI       
BsgI        BsiWI       BsmAI       BsmBI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspLU11I    BsrBI       BsrDI       BsrFI       BsrGI       
BsrI        BssHII      BssKI       Bst1107I    BstAPI      
BstEII      BstF5I      BstNI       BstPI       BstUI       
BstXI       BstZ17I     Bsu36I      CelII       Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        Eam1105I    Eco47III    
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
Fnu4HI      FokI        FseI        FspI        HaeII       
HgaI        Hin1I       HincII      HindII      HinfI       
HpaI        HphI        ItaI        KasI        KpnI        
KspI        MaeIII      MfeI        MluI        MluNI       
MroI        MscI        MspA1I      MunI        MvaI        
MvnI        MwoI        NaeI        NarI        NciI        
NcoI        NdeI        NgoMIV      NotI        NruI        
NsiI        NspI        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        ScrFI       SexAI       SfcI        SfiI        
SfoI        SgrAI       SmaI        SmlI        SpeI        
SphI        Sse8387I    SspBI       SspI        StyI        
SwaI        TfiI        TseI        Tsp45I      Tth111I     
Van91I      XbaI        XcmI        XhoI        XmaCI       
XmaI        


###################################
# ONE CUT ENZYMES (   53 enzymes) #
###################################

AcsI           79  GAATTT               [GA]^AATT[CT]
AfaI          382  GTAC                 GT^AC
Alw44I        160  GTGCAC               G^TGCAC
ApaLI         160  GTGCAC               G^TGCAC
ApoI           79  GAATTT               [GA]^AATT[CT]
Asp700I       355  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI         348  GGATCC               G^GATCC
BclI          140  TGATCA               T^GATCA
BfaI           11  CTAG                 C^TAG
BpmI           70  CTGGAG               CTGGAG(16/14)
BsaAI          34  TACGTA               [CT]AC^GT[GA]
BsaBI         331  GATGTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI          85  CGGCCG               CG[GA][CT]^CG
BspHI         137  TCATGA               T^CATGA
BspMI          23  ACCTGC               ACCTGC(4/8)
BssSI          61  CACGAG               CACGAG(-5/-1)
BstBI          76  TTCGAA               TT^CGAA
BstYI         348  GGATCC               [GA]^GATC[CT]
Cac8I          10  GCTAGC               GC[ACGT]^[ACGT]GC
CfoI           27  GCGC                 GCG^C
DdeI          100  CTTAG                C^T[ACGT]AG
EagI           85  CGGCCG               C^GGCCG
EarI          186  GAAGAG               CTCTTC(1/4)
EclXI          85  CGGCCG               C^GGCCG
Eco52I         85  CGGCCG               C^GGCCG
FbaI          140  TGATCA               T^GATCA
HapII          88  CCGG                 C^CGG
HhaI           27  GCGC                 GCG^C
HinP1I         27  GCGC                 G^CGC
HindIII        97  AAGCTT               A^AGCTT
HpaII          88  CCGG                 C^CGG
Ksp632I       186  GAAGAG               CTCTTC(1/4)
MaeI           11  CTAG                 C^TAG
MaeII          35  ACGT                 A^CGT
MamI          331  GATGTAAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI          348  GGATCC               [GA]^GATC[CT]
MseI          434  TTAA                 T^TAA
MslI          163  CACAAGAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           88  CCGG                 C^CGG
NheI           10  GCTAGC               G^CTAGC
NlaIII        138  CATG                 CATG^
NlaIV         348  GGATCC               GG[ACGT]^[ACGT]CC
NspV           76  TTCGAA               TT^CGAA
RcaI          137  TCATGA               T^CATGA
RsaI          382  GTAC                 GT^AC
SfaNI         226  GATGC                GCATC(5/9)
SfuI           76  TTCGAA               TT^CGAA
SnaBI          34  TACGTA               TAC^GTA
StuI          357  AGGCCT               AGG^CCT
Tru9I         434  TTAA                 T^TAA
TspRI         204  CAGTG                CA[GC]TG[ACGT][ACGT]^
XhoII         348  GGATCC               [GA]^GATC[CT]
XmnI          355  GAAGGCCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AluI           98  AGCT                 AG^CT
              266  AGCT                
AlwI          349  GATCC                GGATC(4/5)
              348  GGATC               
AspHI         160  GTGCAC               G[AT]GC[AT]^C
              289  GAGCAC              
BsiHKAI       160  GTGCAC               G[AT]GC[AT]^C
              289  GAGCAC              
BsiYI         242  CCCCTGATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              243  CCCTGATAGGG         
BslI          242  CCCCTGATAGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              243  CCCTGATAGGG         
DpnII         141  GATC                 ^GATC
              349  GATC                
EaeI           85  CGGCCG               [CT]^GGCC[GA]
              255  TGGCCG              
MboI          141  GATC                 ^GATC
              349  GATC                
MnlI          238  CCTC                 CCTC(7/6)
              151  GAGG                
NdeII         141  GATC                 ^GATC
              349  GATC                
Sau3AI        141  GATC                 ^GATC
              349  GATC                
TaqI           77  TCGA                 T^CGA
              363  TCGA                
TthHB8I        77  TCGA                 T^CGA
              363  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (    5 enzymes) #
#########################################

BmyI        Bsp1286I    HaeIII      MboII       Tsp509I     
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