Restriction map for pF1KB9407
##################################
# NO CUT ENZYMES (   95 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwNI       
Aor51HI     ApaLI       AscI        Asp718I     AspEI       
AviII       AvrII       BamHI       BbeI        BbrPI       
BbvCI       BcgI        BciVI       BfrI        BglI        
BlnI        BsaAI       BsaBI       BseAI       Bsp1407I    
BspDI       BspEI       BspLU11I    BsrGI       BssHII      
BssSI       Bst1107I    BstBI       BstEII      BstPI       
BstXI       BstZ17I     ClaI        CpoI        DrdI        
Eam1105I    Eco47III    EcoO65I     EcoRI       EcoRV       
FseI        FspI        HaeII       HindIII     HpaI        
KasI        KpnI        KspI        MamI        MfeI        
MluI        MroI        MunI        NarI        NcoI        
NdeI        NotI        NruI        NspV        PacI        
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PvuI        RsrII       SacII       SbfI        ScaI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       SpeI        SphI        Sse8387I    
SspBI       SspI        SwaI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcyI         2685  GACGCC               G[GA]^CG[CT]C
AseI         2667  ATTAAT               AT^TAAT
AsnI         2667  ATTAAT               AT^TAAT
AspI         1111  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BglII        1616  AGATCT               A^GATCT
BlpI         3545  GCTTAGC              GC^T[ACGT]AGC
Bpu1102I     3545  GCTTAGC              GC^T[ACGT]AGC
BsaHI        2685  GACGCC               G[GA]^CG[CT]C
BsaWI        3176  ACCGGA               [AT]^CCGG[AT]
BsiWI         125  CGTACG               C^GTACG
BsmBI        3704  GAGACG               CGTCTC(1/5)
BspHI         378  TCATGA               T^CATGA
BsrBI         134  GAGCGG               CCGCTC(-3/-3)
BsrFI        2850  GCCGGC               [GA]^CCGG[CT]
BstAPI       3085  GCAGGCAATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CelII        3545  GCTTAGC              GC^T[ACGT]AGC
Cfr10I       2850  GCCGGC               [GA]^CCGG[CT]
DraI         2380  TTTAAA               TTT^AAA
DraIII       2621  CACTGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI         2981  CCGTGG               C^C[GA][CT]GG
EagI         2643  CGGCCG               C^GGCCG
EclXI        2643  CGGCCG               C^GGCCG
Eco52I       2643  CGGCCG               C^GGCCG
EcoNI        3237  CCTAATCAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I      3092  ATGCAT               ATGCA^T
Hin1I        2685  GACGCC               G[GA]^CG[CT]C
NaeI         2850  GCCGGC               GCC^GGC
NgoMIV       2850  GCCGGC               G^CCGGC
NheI         1783  GCTAGC               G^CTAGC
NsiI         3092  ATGCAT               ATGCA^T
PflFI        1111  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        2420  GACCTATGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI        2667  ATTAAT               AT^TAAT
PvuII         273  CAGCTG               CAG^CTG
RcaI          378  TCATGA               T^CATGA
SalI          648  GTCGAC               G^TCGAC
SapI         2190  GAAGAGC              GCTCTTC(1/4)
StuI         2040  AGGCCT               AGG^CCT
Tth111I      1111  GACCCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XbaI         1176  TCTAGA               T^CTAGA
XcmI          582  CCAGCCAGTCAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   25 enzymes) #
###################################

AccI          648  GTCGAC               GT^[AC][GT]AC
             1135  GTAGAC              
AcsI         2487  GAATTT               [GA]^AATT[CT]
             3599  AAATTT              
AocI         2134  CCTAAGG              CC^T[ACGT]AGG
             2649  CCTGAGG             
ApaI          180  GGGCCC               GGGCC^C
             2390  GGGCCC              
ApoI         2487  GAATTT               [GA]^AATT[CT]
             3599  AAATTT              
BalI         1998  TGGCCA               TGG^CCA
             2358  TGGCCA              
BbsI         1108  GAAGAC               GAAGAC(2/6)
             1152  GAAGAC              
BclI          375  TGATCA               T^GATCA
              602  TGATCA              
BpuAI        1108  GAAGAC               GAAGAC(2/6)
             1152  GAAGAC              
BsiEI         146  CGGTCG               CG[GA][CT]^CG
             2643  CGGCCG              
BsrDI        1676  CATTGC               GCAATG(2/0)
             3089  GCAATG              
Bsu36I       2134  CCTAAGG              CC^T[ACGT]AGG
             2649  CCTGAGG             
Eco81I       2134  CCTAAGG              CC^T[ACGT]AGG
             2649  CCTGAGG             
EcoT14I      2029  CCAAGG               C^C[AT][AT]GG
             3482  CCAAGG              
FbaI          375  TGATCA               T^GATCA
              602  TGATCA              
HgaI         2685  GACGC                GACGC(5/10)
             2440  GCGTC               
HincII        648  GTCGAC               GT[CT]^[GA]AC
             2020  GTTGAC              
HindII        648  GTCGAC               GT[CT]^[GA]AC
             2020  GTTGAC              
MluNI        1998  TGGCCA               TGG^CCA
             2358  TGGCCA              
MscI         1998  TGGCCA               TGG^CCA
             2358  TGGCCA              
PaeR7I        508  CTCGAG               C^TCGAG
             3644  CTCGAG              
PstI          884  CTGCAG               CTGCA^G
             2321  CTGCAG              
SacI          382  GAGCTC               GAGCT^C
             2913  GAGCTC              
StyI         2029  CCAAGG               C^C[AT][AT]GG
             3482  CCAAGG              
XhoI          508  CTCGAG               C^TCGAG
             3644  CTCGAG              


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
Asp700I     AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BfaI        BmyI        
BpmI        BsaI        BsaJI       BseRI       BsgI        
BsiHKAI     BsiYI       BslI        BsmAI       BsmFI       
BsmI        BsoBI       Bsp1286I    BspMI       BsrI        
BssKI       BstF5I      BstNI       BstUI       BstYI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EarI        EcoO109I    EcoRII      
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MseI        
MslI        MspA1I      MspI        MvaI        MvnI        
MwoI        NciI        NdeII       NlaIII      NlaIV       
NspI        PflMI       PleI        PpuMI       RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        TaqI        TfiI        Tru9I       TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I     Van91I      
XhoII       XmnI        
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