Restriction map for
pF1KB9407
(Circular) (Six-base) MAPSORT of: pF1KB9407.seq Check: 5911 from: 1 to: 6887
pF1KB9407 6887 bp
With 182 enzymes: SgfI *
April 24, 2009 13:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2436 2553 2436
Size: 117 6770
AccI GT'mk_AC
Cuts at: 757 1244 3925 757
Size: 487 2681 3719
Fragments arranged by size:
3719 2681 487
Acc65I G'GTAC_C
Cuts at: 3903 3903
Size: 6887
AclI AA'CG_TT
Cuts at: 6727 6727
Size: 6887
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 721 1456 3823 4712 5144 5891 6159 721
Size: 735 2367 889 432 747 268 1449
Fragments arranged by size:
2367 1449 889 747 735 432 268
AflIII A'CryG_T
Cuts at: 4239 5343 6320 4239
Size: 1104 977 4806
Fragments arranged by size:
4806 1104 977
AgeI A'CCGG_T
Cuts at: 5275 5275
Size: 6887
AleI CACnn'nnGTG
Cuts at: 2407 3091 2407
Size: 684 6203
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 2425 2861 3109 2425
Size: 436 248 6203
Fragments arranged by size:
6203 436 248
AlwNI CAG_nnn'CTG
Cuts at: 4192 5759 4192
Size: 1567 5320
ApaI G_GGCC'C
Cuts at: 292 2502 292
Size: 2210 4677
ApaLI G'TGCA_C
Cuts at: 5657 5657
Size: 6887
ApoI r'AATT_y
Cuts at: 2595 3707 3891 6240 6496 2595
Size: 1112 184 2349 256 2986
Fragments arranged by size:
2986 2349 1112 256 184
AseI AT'TA_AT
Cuts at: 20 2776 20
Size: 2756 4131
AsiSI GCG_AT'CGC
Cuts at: 104 104
Size: 6887
AvaI C'yCGr_G
Cuts at: 291 616 2416 3024 3752 3907 291
Size: 325 1800 608 728 155 3271
Fragments arranged by size:
3271 1800 728 608 325 155
BamHI G'GATC_C
Cuts at: 3912 3912
Size: 6887
BanI G'GyrC_C
Cuts at: 646 1106 1747 2141 2898 2919 3903 4317 4565
Size: 460 641 394 757 21 984 414 248
Cuts at: 4565 4600 646
Size: 35 2933
Fragments arranged by size:
2933 984 757 641 460 414 394 248
35 21
BanII G_rGCy'C
Cuts at: 292 494 2305 2502 3025 3250 3901 4931 292
Size: 202 1811 197 523 225 651 1030 2248
Fragments arranged by size:
2248 1811 1030 651 523 225 202 197
BbeI G_GCGC'C
Cuts at: 4321 4569 4321
Size: 248 6639
BbsI GAAGACnn'nnnn_
Cuts at: 1223 1267 1223
Size: 44 6843
BciVI GTATCCnnnnn_n'
Cuts at: 4785 5546 6887 4785
Size: 761 1341 4785
Fragments arranged by size:
4785 1341 761
BclI T'GATC_A
Cuts at: 483 710 4407 483
Size: 227 3697 2963
Fragments arranged by size:
3697 2963 227
BfrBI ATG'CAT
Cuts at: 3202 3202
Size: 6887
BglII A'GATC_T
Cuts at: 1724 4402 6439 1724
Size: 2678 2037 2172
Fragments arranged by size:
2678 2172 2037
BlpI GC'TnA_GC
Cuts at: 3654 3999 3654
Size: 345 6542
Bme1580I G_kGCm'C
Cuts at: 292 2502 2903 2924 4512 4605 5661 292
Size: 2210 401 21 1588 93 1056 1518
Fragments arranged by size:
2210 1588 1518 1056 401 93 21
BmgBI CAC'GTC
Cuts at: 363 1959 2862 3108 363
Size: 1596 903 246 4142
Fragments arranged by size:
4142 1596 903 246
BmrI ACTGGGnnnn_n'
Cuts at: 1035 2496 2740 4099 4180 4515 6659 1035
Size: 1461 244 1359 81 335 2144 1263
Fragments arranged by size:
2144 1461 1359 1263 335 244 81
BmtI G_CTAG'C
Cuts at: 1895 1895
Size: 6887
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 647 2940 5254 647
Size: 2293 2314 2280
Fragments arranged by size:
2314 2293 2280
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 478 1585 2429 2667 2786 3863 340
Size: 138 1107 844 238 119 1077 3364
Fragments arranged by size:
3364 1107 1077 844 238 138 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1330 1697 4063 4942 5434 5732 1330
Size: 367 2366 879 492 298 2485
Fragments arranged by size:
2485 2366 879 492 367 298
BsaI GGTCTCn'nnnn_
Cuts at: 482 1867 3275 3721 6591 482
Size: 1385 1408 446 2870 778
Fragments arranged by size:
2870 1408 1385 778 446
BsaAI yAC'GTr
Cuts at: 4240 4240
Size: 6887
BsaBI GATnn'nnATC
Cuts at: 6 4401 6438 6
Size: 4395 2037 455
Fragments arranged by size:
4395 2037 455
BsaHI Gr'CG_yC
Cuts at: 2794 4318 4566 5268 6764 2794
Size: 1524 248 702 1496 2917
Fragments arranged by size:
2917 1524 1496 702 248
BsaWI w'CCGG_w
Cuts at: 3284 4076 4597 5275 5549 5696 6107 3284
Size: 792 521 678 274 147 411 4064
Fragments arranged by size:
4064 792 678 521 411 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 217 247 534 564 2396 2426 217
Size: 30 287 30 1832 30 4678
Fragments arranged by size:
4678 1832 287 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 838 841 901 1183 2291 2925 3599 3629 838
Size: 3 60 282 1108 634 674 30 4096
Fragments arranged by size:
4096 1108 674 634 282 60 30 3
BseYI C'CCAG_C
Cuts at: 189 204 297 366 1950 4312 5070 5647 6143
Size: 15 93 69 1584 2362 758 577 496
Cuts at: 6143 189
Size: 933
Fragments arranged by size:
2362 1584 933 758 577 496 93 69
15
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 346 2215 2362 3115 346
Size: 1869 147 753 4118
Fragments arranged by size:
4118 1869 753 147
BsiEI CG_ry'CG
Cuts at: 104 257 2754 4429 4475 5683 104
Size: 153 2497 1675 46 1208 1308
Fragments arranged by size:
2497 1675 1308 1208 153 46
BsiHKAI G_wGCw'C
Cuts at: 221 494 893 2414 3025 3901 4679 4869 5661
Size: 273 399 1521 611 876 778 190 792
Cuts at: 5661 221
Size: 1447
Fragments arranged by size:
1521 1447 876 792 778 611 399 273
190
BsiWI C'GTAC_G
Cuts at: 233 233
Size: 6887
BsmI GAATG_Cn'
Cuts at: 163 3411 3747 163
Size: 3248 336 3303
Fragments arranged by size:
3303 3248 336
BsmBI CGTCTCn'nnnn_
Cuts at: 3806 3806
Size: 6887
Bsp1286I G_dGCh'C
Cuts at: 221 292 494 893 2305 2414 2502 2903 2924
Size: 71 202 399 1412 109 88 401 21
Cuts at: 2924 3025 3250 3901 4512 4605 4679 4869 4931
Size: 101 225 651 611 93 74 190 62
Cuts at: 4931 5661 221
Size: 730 1447
Fragments arranged by size:
1447 1412 730 651 611 401 399 225
202 190 109 101 93 88 74 71
62 21
BspEI T'CCGG_A
Cuts at: 6107 6107
Size: 6887
BspHI T'CATG_A
Cuts at: 486 6063 486
Size: 5577 1310
BspMI ACCTGCnnnn'nnnn_
Cuts at: 449 2436 2553 3937 4151 4453 4834 449
Size: 1987 117 1384 214 302 381 2502
Fragments arranged by size:
2502 1987 1384 381 302 214 117
BsrBI CCG'CTC
Cuts at: 50 244 5179 5233 6717 6882 50
Size: 194 4935 54 1484 165 55
Fragments arranged by size:
4935 1484 194 165 55 54
BsrDI GCAATG_nn'
Cuts at: 1783 3204 4799 1783
Size: 1421 1595 3871
Fragments arranged by size:
3871 1595 1421
BsrFI r'CCGG_y
Cuts at: 2958 4885 5066 5275 2958
Size: 1927 181 209 4570
Fragments arranged by size:
4570 1927 209 181
BssSI C'ACGA_G
Cuts at: 5158 5516 5158
Size: 358 6529
BstAPI GCAn_nnn'nTGC
Cuts at: 3199 4264 3199
Size: 1065 5822
BstBI TT'CG_AA
Cuts at: 5248 5248
Size: 6887
BstYI r'GATC_y
Cuts at: 1 1724 2163 2481 3113 3287 3912 4379 4402
Size: 1723 439 318 632 174 625 467 23
Cuts at: 4402 4737 4983 5995 6081 6093 6439 1
Size: 335 246 1012 86 12 346 449
Fragments arranged by size:
1723 1012 632 625 467 449 439 346
335 318 246 174 86 23 12
Bsu36I CC'TnA_GG
Cuts at: 2243 2758 2243
Size: 515 6372
BtgI C'CryG_G
Cuts at: 3089 3089
Size: 6887
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 921 4837 5018 5181 921
Size: 3916 181 163 2627
Fragments arranged by size:
3916 2627 181 163
BtsI GCAGTG_nn'
Cuts at: 3060 4130 3060
Size: 1070 5817
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 2580 2615 2580
Size: 35 6852
DraI TTT'AAA
Cuts at: 2490 3886 2490
Size: 1396 5491
DraIII CAC_nnn'GTG
Cuts at: 2734 2734
Size: 6887
DrdI GACnn_nn'nnGTC
Cuts at: 4593 5451 4593
Size: 858 6029
EaeI y'GGCC_r
Cuts at: 2106 2466 2751 3780 4472 4646 5037 5064 6409
Size: 360 285 1029 692 174 391 27 1345
Cuts at: 6409 2106
Size: 2584
Fragments arranged by size:
2584 1345 1029 692 391 360 285 174
27
EagI C'GGCC_G
Cuts at: 2751 4472 2751
Size: 1721 5166
EarI CTCTTCn'nnn_
Cuts at: 638 1130 2293 2969 4910 5120 638
Size: 492 1163 676 1941 210 2405
Fragments arranged by size:
2405 1941 1163 676 492 210
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 5095 5405 5551 6697 5095
Size: 310 146 1146 5285
Fragments arranged by size:
5285 1146 310 146
EcoICRI GAG'CTC
Cuts at: 492 3023 3899 492
Size: 2531 876 3480
Fragments arranged by size:
3480 2531 876
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 340 478 721 1456 1585 2429 2667 2786 3823
Size: 138 243 735 129 844 238 119 1037
Cuts at: 3823 3863 4712 5144 5891 6159 340
Size: 40 849 432 747 268 1068
Fragments arranged by size:
1068 1037 849 844 747 735 432 268
243 238 138 129 119 40
EcoNI CCTnn'n_nnAGG
Cuts at: 3349 3349
Size: 6887
EcoO109I rG'GnC_Cy
Cuts at: 175 288 438 1009 2199 3476 4026 175
Size: 113 150 571 1190 1277 550 3036
Fragments arranged by size:
3036 1277 1190 571 550 150 113
EcoRI G'AATT_C
Cuts at: 3891 3891
Size: 6887
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 624 1486 4906 624
Size: 862 3420 2605
Fragments arranged by size:
3420 2605 862
FspI TGC'GCA
Cuts at: 4668 4668
Size: 6887
HaeII r_GCGC'y
Cuts at: 4321 4569 5591 6570 4321
Size: 248 1022 979 4638
Fragments arranged by size:
4638 1022 979 248
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 437 469 1380 1412 3272 3296 3304 3328 3385
Size: 32 911 32 1860 24 8 24 57
Cuts at: 3385 3417 4729 4759 4761 4791 437
Size: 32 1312 30 2 30 2533
Fragments arranged by size:
2533 1860 1312 911 57 32 32 32
30 30 24 24 8 2
HincII GTy'rAC
Cuts at: 758 2130 3926 758
Size: 1372 1796 3719
Fragments arranged by size:
3719 1796 1372
KasI G'GCGC_C
Cuts at: 4317 4565 4317
Size: 248 6639
KpnI G_GTAC'C
Cuts at: 3907 3907
Size: 6887
MluI A'CGCG_T
Cuts at: 6320 6320
Size: 6887
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 219 1554 3211 5558 5742 219
Size: 1335 1657 2347 184 1364
Fragments arranged by size:
2347 1657 1364 1335 184
MscI TGG'CCA
Cuts at: 2108 2468 4648 2108
Size: 360 2180 4347
Fragments arranged by size:
4347 2180 360
MslI CAynn'nnrTG
Cuts at: 1706 2407 3091 4876 5003 1706
Size: 701 684 1785 127 3590
Fragments arranged by size:
3590 1785 701 684 127
MspA1I CmG'CkG
Cuts at: 265 383 1954 3032 3044 3074 3260 3998 4312
Size: 118 1571 1078 12 30 186 738 314
Cuts at: 4312 4672 5685 5930 265
Size: 360 1013 245 1222
Fragments arranged by size:
1571 1222 1078 1013 738 360 314 245
186 118 30 12
NaeI GCC'GGC
Cuts at: 2960 5068 2960
Size: 2108 4779
NarI GG'CG_CC
Cuts at: 4318 4566 4318
Size: 248 6639
NgoMIV G'CCGG_C
Cuts at: 2958 5066 2958
Size: 2108 4779
NheI G'CTAG_C
Cuts at: 1891 1891
Size: 6887
NsiI A_TGCA'T
Cuts at: 3204 3204
Size: 6887
NspI r_CATG'y
Cuts at: 1711 2517 3206 3940 4440 5347 1711
Size: 806 689 734 500 907 3251
Fragments arranged by size:
3251 907 806 734 689 500
PasI CC'CwG_GG
Cuts at: 180 180
Size: 6887
PciI A'CATG_T
Cuts at: 5343 5343
Size: 6887
PflMI CCAn_nnn'nTGG
Cuts at: 700 2220 2803 2823 3832 700
Size: 1520 583 20 1009 3755
Fragments arranged by size:
3755 1520 1009 583 20
PfoI T'CCnGG_A
Cuts at: 2506 2506
Size: 6887
PmeI GTTT'AAAC
Cuts at: 3886 3886
Size: 6887
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6026 6058 6026
Size: 32 6855
PpuMI rG'GwC_Cy
Cuts at: 175 438 1009 3476 175
Size: 263 571 2467 3586
Fragments arranged by size:
3586 2467 571 263
PshAI GACnn'nnGTC
Cuts at: 2532 2532
Size: 6887
PspOMI G'GGCC_C
Cuts at: 288 2498 288
Size: 2210 4677
PspXI vC'TCGA_Gb
Cuts at: 616 616
Size: 6887
PstI C_TGCA'G
Cuts at: 996 2433 3934 4619 996
Size: 1437 1501 685 3264
Fragments arranged by size:
3264 1501 1437 685
PvuI CG_AT'CG
Cuts at: 104 104
Size: 6887
PvuII CAG'CTG
Cuts at: 383 4312 4672 383
Size: 3929 360 2598
Fragments arranged by size:
3929 2598 360
RsrII CG'GwC_CG
Cuts at: 5082 5082
Size: 6887
SacI G_AGCT'C
Cuts at: 494 3025 3901 494
Size: 2531 876 3480
Fragments arranged by size:
3480 2531 876
SalI G'TCGA_C
Cuts at: 756 3924 756
Size: 3168 3719
SanDI GG'GwC_CC
Cuts at: 175 3476 175
Size: 3301 3586
SapI GCTCTTCn'nnn_
Cuts at: 2293 4910 5120 2293
Size: 2617 210 4060
Fragments arranged by size:
4060 2617 210
SbfI CC_TGCA'GG
Cuts at: 3934 3934
Size: 6887
ScaI AGT'ACT
Cuts at: 6404 6404
Size: 6887
SfcI C'TryA_G
Cuts at: 33 992 2429 3758 3930 4615 5608 5799 33
Size: 959 1437 1329 172 685 993 191 1121
Fragments arranged by size:
1437 1329 1121 993 959 685 191 172
SfoI GGC'GCC
Cuts at: 4319 4567 4319
Size: 248 6639
SgfI GCG_AT'CGC
Cuts at: 104 104
Size: 6887
SmaI CCC'GGG
Cuts at: 3909 3909
Size: 6887
SmlI C'TyrA_G
Cuts at: 616 1309 1712 3752 4042 4957 5449 5711 616
Size: 693 403 2040 290 915 492 262 1792
Fragments arranged by size:
2040 1792 915 693 492 403 290 262
SpeI A'CTAG_T
Cuts at: 67 67
Size: 6887
SphI G_CATG'C
Cuts at: 3940 4440 3940
Size: 500 6387
StuI AGG'CCT
Cuts at: 2150 2150
Size: 6887
StyI C'CwwG_G
Cuts at: 2137 3590 4021 4375 2137
Size: 1453 431 354 4649
Fragments arranged by size:
4649 1453 431 354
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 690 2671 2722 4470 4603 5062 5271 690
Size: 1981 51 1748 133 459 209 2306
Fragments arranged by size:
2306 1981 1748 459 209 133 51
TatI w'GTAC_w
Cuts at: 6402 6402
Size: 6887
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2344 2371 2376 2396 2403 2428 2667 2699 6404
Size: 27 5 20 7 25 239 32 3705
Cuts at: 6404 6436 2344
Size: 32 2795
Fragments arranged by size:
3705 2795 239 32 32 27 25 20
7 5
Tth111I GACn'n_nGTC
Cuts at: 1222 4684 1222
Size: 3462 3425
XbaI T'CTAG_A
Cuts at: 1284 3918 1284
Size: 2634 4253
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 697 4382 697
Size: 3685 3202
XhoI C'TCGA_G
Cuts at: 616 3752 616
Size: 3136 3751
XmaI C'CCGG_G
Cuts at: 3907 3907
Size: 6887
XmnI GAAnn'nnTTC
Cuts at: 604 1500 1726 3854 604
Size: 896 226 2128 3637
Fragments arranged by size:
3637 2128 896 226
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AflIII AgeI AleI
AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI
BamHI BanI BanII BbeI BbsI BciVI BclI BfrBI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BssSI BstAPI
BstBI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII
KasI KpnI MluI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NheI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PspOMI PspXI PstI
PvuI PvuII RsrII SacI SalI SanDI SapI SbfI
ScaI SfcI SfoI SgfI SmaI SmlI SpeI SphI
StuI StyI TaqII TatI TstI Tth111I XbaI XcmI
XhoI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AhdI AloI AscI AvrII BaeI
BbvCI BcgI BglI Bpu10I BsrGI BssHII BstEII BstXI
BstZ17I ClaI EcoRV FseI FspAI HindIII HpaI MfeI
NcoI NdeI NotI NruI PacI PmlI PsiI PsrI
SacII SexAI SfiI SgrAI SnaBI SrfI SspI SwaI
ZraI