Restriction map for
pF1KB8018
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AhdI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BalI BamHI BbsI BcgI BclI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BseAI BsgI BsiWI BsmI
BspDI BspEI BspHI BspLU11I BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HgaI HindIII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PmeI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI XbaI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AcyI 385 GGCGCC G[GA]^CG[CT]C
AflII 865 CTTAAG C^TTAAG
AflIII 702 ACACGT A^C[GA][CT]GT
AgeI 1445 ACCGGT A^CCGGT
Alw44I 209 GTGCAC G^TGCAC
AocI 1132 CCTAAGG CC^T[ACGT]AGG
ApaLI 209 GTGCAC G^TGCAC
AspI 766 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 385 GGCGCC G^G[CT][GA]CC
BbeI 385 GGCGCC GGCGC^C
BbrPI 703 CACGTG CAC^GTG
BbvCI 1589 CCTCAGC CCTCAGC(-5/-2)
BciVI 990 GGATAC GTATCC(6/5)
BfaI 47 CTAG C^TAG
BfrI 865 CTTAAG C^TTAAG
BsaAI 703 CACGTG [CT]AC^GT[GA]
BsaHI 385 GGCGCC G[GA]^CG[CT]C
BsiEI 1463 CGACCG CG[GA][CT]^CG
BsmAI 1179 GAGAC GTCTC(1/5)
BsmBI 1179 GAGACG CGTCTC(1/5)
BspMI 1508 GCAGGT ACCTGC(4/8)
BstAPI 114 GCAGCTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 26 CCAAACAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1132 CCTAAGG CC^T[ACGT]AGG
DraI 558 TTTAAA TTT^AAA
DsaI 1285 CCACGG C^C[GA][CT]GG
EarI 1423 GAAGAG CTCTTC(1/4)
Eco81I 1132 CCTAAGG CC^T[ACGT]AGG
EcoNI 655 CCTGGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1152 GAATTC G^AATTC
EcoT14I 392 CCAAGG C^C[AT][AT]GG
Hin1I 385 GGCGCC G[GA]^CG[CT]C
HincII 1656 GTTGAC GT[CT]^[GA]AC
HindII 1656 GTTGAC GT[CT]^[GA]AC
KasI 385 GGCGCC G^GCGCC
Ksp632I 1423 GAAGAG CTCTTC(1/4)
MaeI 47 CTAG C^TAG
MaeII 704 ACGT A^CGT
NarI 385 GGCGCC GG^CGCC
PaeR7I 1349 CTCGAG C^TCGAG
PflFI 766 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1445 ACCGGT A^CCGGT
PmaCI 703 CACGTG CAC^GTG
PmlI 703 CACGTG CAC^GTG
PpuMI 137 AGGACCT [GA]G^G[AT]CC[CT]
SfoI 385 GGCGCC GGC^GCC
SmaI 1067 CCCGGG CCC^GGG
StuI 663 AGGCCT AGG^CCT
StyI 392 CCAAGG C^C[AT][AT]GG
Tth111I 766 GACGAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1349 CTCGAG C^TCGAG
XmaCI 1067 CCCGGG C^CCGGG
XmaI 1067 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 960 CGCG CG^CG
1643 CGCG
AcsI 726 AAATTC [GA]^AATT[CT]
1152 GAATTC
ApoI 726 AAATTC [GA]^AATT[CT]
1152 GAATTC
Asp700I 769 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1152 GAATTCCTTC
AvaI 1067 CCCGGG C^[CT]CG[GA]G
1349 CTCGAG
BglI 388 GCCGCCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
397 GCCCTGCTGGC
BglII 742 AGATCT A^GATCT
1527 AGATCT
BsaWI 847 ACCGGA [AT]^CCGG[AT]
1445 ACCGGT
BsoBI 1067 CCCGGG C^[CT]CG[GA]G
1349 CTCGAG
Bsp1407I 674 TGTACA T^GTACA
939 TGTACA
BsrBI 1402 GAGCGG CCGCTC(-3/-3)
1468 GAGCGG
BsrDI 1121 CATTGC GCAATG(2/0)
1194 CATTGC
BsrFI 1385 GCCGGT [GA]^CCGG[CT]
1445 ACCGGT
BsrGI 674 TGTACA T^GTACA
939 TGTACA
BstUI 960 CGCG CG^CG
1643 CGCG
Cfr10I 1385 GCCGGT [GA]^CCGG[CT]
1445 ACCGGT
MslI 757 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1248 CACAAAAATG
MvnI 960 CGCG CG^CG
1643 CGCG
NspI 619 GCATGT [GA]CATG^[CT]
671 GCATGT
PflMI 1120 CCATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1270 CCATCTTATGG
PstI 121 CTGCAG CTGCA^G
734 CTGCAG
SacI 376 GAGCTC GAGCT^C
1428 GAGCTC
SmlI 865 CTTAAG C^T[CT][GA]AG
1349 CTCGAG
SspBI 674 TGTACA T^GTACA
939 TGTACA
TaqI 227 TCGA T^CGA
1350 TCGA
TthHB8I 227 TCGA T^CGA
1350 TCGA
Van91I 1120 CCATTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1270 CCATCTTATGG
XcmI 26 CCAAACAAGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
392 CCAAGGCCCTGCTGG
XmnI 769 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1152 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI EcoO109I EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII