Restriction map for pF1KB8018
 (Circular) (Six-base) MAPSORT of: pF1KB8018.seq  Check: 774  from: 1  to: 4778



pF1KB8018  4778 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:08  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   1607    1607 
   Size:       4778
 
AccI GT'mk_AC

Cuts at:   1816    1816 
   Size:       4778
 
Acc65I G'GTAC_C

Cuts at:   1794    1794 
   Size:       4778
 
AclI AA'CG_TT

Cuts at:   4618    4618 
   Size:       4778
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1129    2603    3035    3782    4050    1129 
   Size:       1474     432     747     268    1857

  Fragments arranged by size: 

                1857    1474     747     432     268
 
AflII C'TTAA_G

Cuts at:    973     973 
   Size:       4778
 
AflIII A'CryG_T

Cuts at:    810    2130    3234    4211     810 
   Size:       1320    1104     977    1377

  Fragments arranged by size: 

                1377    1320    1104     977
 
AgeI A'CCGG_T

Cuts at:   1553    3166    1553 
   Size:       1613    3165
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1715    1747    1715 
   Size:         32    4746
 
AlwNI CAG_nnn'CTG

Cuts at:    228     249     709    2083    3650     228 
   Size:         21     460    1374    1567    1356

  Fragments arranged by size: 

                1567    1374    1356     460      21
 
ApaLI G'TGCA_C

Cuts at:    317    3548     317 
   Size:       3231    1547
 
ApoI r'AATT_y

Cuts at:    834    1260    1782    4131    4387     834 
   Size:        426     522    2349     256    1225

  Fragments arranged by size: 

                2349    1225     522     426     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4778
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4778
 
AvaI C'yCGr_G

Cuts at:   1175    1457    1798    1175 
   Size:        282     341    4155

  Fragments arranged by size: 

                4155     341     282
 
BamHI G'GATC_C

Cuts at:   1803    1803 
   Size:       4778
 
BanI G'GyrC_C

Cuts at:    493    1794    2208    2456    2491     493 
   Size:       1301     414     248      35    2780

  Fragments arranged by size: 

                2780    1301     414     248      35
 
BanII G_rGCy'C

Cuts at:    133     488     531    1540    1792    2822     133 
   Size:        355      43    1009     252    1030    2089

  Fragments arranged by size: 

                2089    1030    1009     355     252      43
 
BbeI G_GCGC'C

Cuts at:    497    2212    2460     497 
   Size:       1715     248    2815

  Fragments arranged by size: 

                2815    1715     248
 
BbvCI CC'TCA_GC

Cuts at:   1698    1698 
   Size:       4778
 
BciVI GTATCCnnnnn_n'

Cuts at:   1092    2676    3437    4778    1092 
   Size:       1584     761    1341    1092

  Fragments arranged by size: 

                1584    1341    1092     761
 
BclI T'GATC_A

Cuts at:   2298    2298 
   Size:       4778
 
BglI GCCn_nnn'nGGC

Cuts at:    502     511     502 
   Size:          9    4769
 
BglII A'GATC_T

Cuts at:    850    1635    2293    4330     850 
   Size:        785     658    2037    1298

  Fragments arranged by size: 

                2037    1298     785     658
 
BlpI GC'TnA_GC

Cuts at:   1890    1890 
   Size:       4778
 
Bme1580I G_kGCm'C

Cuts at:    321    2403    2496    3552     321 
   Size:       2082      93    1056    1547

  Fragments arranged by size: 

                2082    1547    1056      93
 
BmrI ACTGGGnnnn_n'

Cuts at:   1071    1990    2071    2406    4550    1071 
   Size:        919      81     335    2144    1299

  Fragments arranged by size: 

                2144    1299     919     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1557    1559    3145    1557 
   Size:          2    1586    3190

  Fragments arranged by size: 

                3190    1586       2
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    271     754    1545     271 
   Size:        483     791    3504

  Fragments arranged by size: 

                3504     791     483
 
Bpu10I CC'TnA_GC

Cuts at:   1698    1698 
   Size:       4778
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1954    2833    3325    3623    1954 
   Size:        879     492     298    3109

  Fragments arranged by size: 

                3109     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4482    4482 
   Size:       4778
 
BsaAI yAC'GTr

Cuts at:    813    2131     813 
   Size:       1318    3460
 
BsaBI GATnn'nnATC

Cuts at:      6    2292    4329       6 
   Size:       2286    2037     455

  Fragments arranged by size: 

                2286    2037     455
 
BsaHI Gr'CG_yC

Cuts at:    494    2209    2457    3159    4655     494 
   Size:       1715     248     702    1496     617

  Fragments arranged by size: 

                1715    1496     702     617     248
 
BsaWI w'CCGG_w

Cuts at:    955    1553    1967    2488    3166    3440    3587    3998     955 
   Size:        598     414     521     678     274     147     411    1735

  Fragments arranged by size: 

                1735     678     598     521     414     411     274     147
            
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    277     307     996    1026    1044    1074    1543    1573    1715 
   Size:         30     689      30      18      30     469      30     142

Cuts at:   1715    1745     277 
   Size:         30    3310

  Fragments arranged by size: 

                3310     689     469     142      30      30      30      30
                  30      18
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    268    1139    1530    1686     268 
   Size:        871     391     156    3360

  Fragments arranged by size: 

                3360     871     391     156
 
BseYI C'CCAG_C

Cuts at:    258    2203    2961    3538    4034     258 
   Size:       1945     758     577     496    1002

  Fragments arranged by size: 

                1945    1002     758     577     496
 
BsiEI CG_ry'CG

Cuts at:    104    1574    2320    2366    3574     104 
   Size:       1470     746      46    1208    1308

  Fragments arranged by size: 

                1470    1308    1208     746      46
 
BsiHKAI G_wGCw'C

Cuts at:    321     488    1540    1792    2570    2760    3552     321 
   Size:        167    1052     252     778     190     792    1547

  Fragments arranged by size: 

                1547    1052     792     778     252     190     167
 
BsmBI CGTCTCn'nnnn_

Cuts at:   1281    1281 
   Size:       4778
 
Bsp1286I G_dGCh'C

Cuts at:    133     321     488     531    1540    1792    2403    2496    2570 
   Size:        188     167      43    1009     252     611      93      74

Cuts at:   2570    2760    2822    3552     133 
   Size:        190      62     730    1359

  Fragments arranged by size: 

                1359    1009     730     611     252     190     188     167
                  93      74      62      43
 
BspEI T'CCGG_A

Cuts at:   3998    3998 
   Size:       4778
 
BspHI T'CATG_A

Cuts at:   3954    3954 
   Size:       4778
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:   1607    1828    2042    2344    2725    1607 
   Size:        221     214     302     381    3660

  Fragments arranged by size: 

                3660     381     302     221     214
 
BsrBI CCG'CTC

Cuts at:     50    1512    1578    3070    3124    4608    4773      50 
   Size:       1462      66    1492      54    1484     165      55

  Fragments arranged by size: 

                1492    1484    1462     165      66      55      54
 
BsrDI GCAATG_nn'

Cuts at:   1228    1301    2690    1228 
   Size:         73    1389    3316

  Fragments arranged by size: 

                3316    1389      73
 
BsrFI r'CCGG_y

Cuts at:   1493    1553    2776    2957    3166    1493 
   Size:         60    1223     181     209    3105

  Fragments arranged by size: 

                3105    1223     209     181      60
 
BsrGI T'GTAC_A

Cuts at:    782    1047     782 
   Size:        265    4513
 
BssSI C'ACGA_G

Cuts at:   3049    3407    3049 
   Size:        358    4420
 
BstAPI GCAn_nnn'nTGC

Cuts at:    228    2155     228 
   Size:       1927    2851
 
BstBI TT'CG_AA

Cuts at:   3139    3139 
   Size:       4778
 
BstXI CCAn_nnnn'nTGG

Cuts at:    141     141 
   Size:       4778
 
BstYI r'GATC_y

Cuts at:      1     705     850    1134    1635    1803    2270    2293    2628 
   Size:        704     145     284     501     168     467      23     335

Cuts at:   2628    2874    3886    3972    3984    4330       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     704     501     467     449     346     335     284
                 246     168     145      86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:   1241    1241 
   Size:       4778
 
BtgI C'CryG_G

Cuts at:    107    1393     107 
   Size:       1286    3492
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2728    2909    3072    2728 
   Size:        181     163    4434

  Fragments arranged by size: 

                4434     181     163
 
BtsI GCAGTG_nn'

Cuts at:   2021    2021 
   Size:       4778
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    942     977    1382    1417     942 
   Size:         35     405      35    4303

  Fragments arranged by size: 

                4303     405      35      35
 
DraI TTT'AAA

Cuts at:    668    1777     668 
   Size:       1109    3669
 
DrdI GACnn_nn'nnGTC

Cuts at:    419    1077    1460    2484    3342     419 
   Size:        658     383    1024     858    1855

  Fragments arranged by size: 

                1855    1024     858     658     383
 
EaeI y'GGCC_r

Cuts at:   2363    2537    2928    2955    4300    2363 
   Size:        174     391      27    1345    2841

  Fragments arranged by size: 

                2841    1345     391     174      27
 
EagI C'GGCC_G

Cuts at:   2363    2363 
   Size:       4778
 
EarI CTCTTCn'nnn_

Cuts at:   1526    2801    3011    1526 
   Size:       1275     210    3293

  Fragments arranged by size: 

                3293    1275     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   1142    2986    3296    3442    4588    1142 
   Size:       1844     310     146    1146    1332

  Fragments arranged by size: 

                1844    1332    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    486    1538    1790     486 
   Size:       1052     252    3474

  Fragments arranged by size: 

                3474    1052     252
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    271     754    1129    1545    2603    3035    3782    4050     271 
   Size:        483     375     416    1058     432     747     268     999

  Fragments arranged by size: 

                1058     999     747     483     432     416     375     268
            
 
EcoNI CCTnn'n_nnAGG

Cuts at:    767     767 
   Size:       4778
 
EcoO109I rG'GnC_Cy

Cuts at:    246     453     504    1917     246 
   Size:        207      51    1413    3107

  Fragments arranged by size: 

                3107    1413     207      51
 
EcoRI G'AATT_C

Cuts at:   1260    1782    1260 
   Size:        522    4256
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    443     636    2797     443 
   Size:        193    2161    2424

  Fragments arranged by size: 

                2424    2161     193
 
FspI TGC'GCA

Cuts at:   2559    2559 
   Size:       4778
 
HaeII r_GCGC'y

Cuts at:    497     984    1652    2212    2460    3482    4461     497 
   Size:        487     668     560     248    1022     979     814

  Fragments arranged by size: 

                1022     979     814     668     560     487     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    194     225     226     257     933     965    1044    1076    2620 
   Size:         31       1      31     676      32      79      32    1544

Cuts at:   2620    2650    2652    2682     194 
   Size:         30       2      30    2290

  Fragments arranged by size: 

                2290    1544     676      79      32      32      31      31
                  30      30       2       1
 
HincII GTy'rAC

Cuts at:   1766    1817    1766 
   Size:         51    4727
 
KasI G'GCGC_C

Cuts at:    493    2208    2456     493 
   Size:       1715     248    2815

  Fragments arranged by size: 

                2815    1715     248
 
KpnI G_GTAC'C

Cuts at:   1798    1798 
   Size:       4778
 
MluI A'CGCG_T

Cuts at:   4211    4211 
   Size:       4778
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    143     275    3449    3633     143 
   Size:        132    3174     184    1288

  Fragments arranged by size: 

                3174    1288     184     132
 
MscI TGG'CCA

Cuts at:   2539    2539 
   Size:       4778
 
MslI CAynn'nnrTG

Cuts at:    869    1360    2767    2894     869 
   Size:        491    1407     127    2753

  Fragments arranged by size: 

                2753    1407     491     127
 
MspA1I CmG'CkG

Cuts at:    225     918    1742    1889    2203    2563    3576    3821     225 
   Size:        693     824     147     314     360    1013     245    1182

  Fragments arranged by size: 

                1182    1013     824     693     360     314     245     147
            
 
NaeI GCC'GGC

Cuts at:   2959    2959 
   Size:       4778
 
NarI GG'CG_CC

Cuts at:    494    2209    2457     494 
   Size:       1715     248    2815

  Fragments arranged by size: 

                2815    1715     248
 
NcoI C'CATG_G

Cuts at:    107     107 
   Size:       4778
 
NgoMIV G'CCGG_C

Cuts at:   2957    2957 
   Size:       4778
 
NspI r_CATG'y

Cuts at:    731     783    1831    2331    3238     731 
   Size:         52    1048     500     907    2271

  Fragments arranged by size: 

                2271    1048     907     500      52
 
PciI A'CATG_T

Cuts at:   3234    3234 
   Size:       4778
 
PflMI CCAn_nnn'nTGG

Cuts at:   1234    1384    1234 
   Size:        150    4628
 
PfoI T'CCnGG_A

Cuts at:   1130    1664    1130 
   Size:        534    4244
 
PmeI GTTT'AAAC

Cuts at:   1777    1777 
   Size:       4778
 
PmlI CAC'GTG

Cuts at:    813     813 
   Size:       4778
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1715    1747    3917    3949    1715 
   Size:         32    2170      32    2544

  Fragments arranged by size: 

                2544    2170      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    246     246 
   Size:       4778
 
PspXI vC'TCGA_Gb

Cuts at:   1457    1457 
   Size:       4778
 
PstI C_TGCA'G

Cuts at:    233     846    1825    2510     233 
   Size:        613     979     685    2501

  Fragments arranged by size: 

                2501     979     685     613
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4778
 
PvuII CAG'CTG

Cuts at:    225     918    1742    2203    2563     225 
   Size:        693     824     461     360    2440

  Fragments arranged by size: 

                2440     824     693     461     360
 
RsrII CG'GwC_CG

Cuts at:   2973    2973 
   Size:       4778
 
SacI G_AGCT'C

Cuts at:    488    1540    1792     488 
   Size:       1052     252    3474

  Fragments arranged by size: 

                3474    1052     252
 
SalI G'TCGA_C

Cuts at:   1815    1815 
   Size:       4778
 
SapI GCTCTTCn'nnn_

Cuts at:   2801    3011    2801 
   Size:        210    4568
 
SbfI CC_TGCA'GG

Cuts at:   1825    1825 
   Size:       4778
 
ScaI AGT'ACT

Cuts at:   4295    4295 
   Size:       4778
 
SfcI C'TryA_G

Cuts at:     33     229     602     842    1821    2506    3499    3690      33 
   Size:        196     373     240     979     685     993     191    1121

  Fragments arranged by size: 

                1121     993     979     685     373     240     196     191
            
 
SfoI GGC'GCC

Cuts at:    495    2210    2458     495 
   Size:       1715     248    2815

  Fragments arranged by size: 

                2815    1715     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4778
 
SmaI CCC'GGG

Cuts at:   1177    1800    1177 
   Size:        623    4155
 
SmlI C'TyrA_G

Cuts at:    973    1457    1933    2848    3340    3602     973 
   Size:        484     476     915     492     262    2149

  Fragments arranged by size: 

                2149     915     492     484     476     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4778
 
SphI G_CATG'C

Cuts at:   1831    2331    1831 
   Size:        500    4278
 
StuI AGG'CCT

Cuts at:    773     773 
   Size:       4778
 
StyI C'CwwG_G

Cuts at:    107     500    1912    2266     107 
   Size:        393    1412     354    2619

  Fragments arranged by size: 

                2619    1412     393     354
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1588    2361    2494    2953    3162    1588 
   Size:        773     133     459     209    3204

  Fragments arranged by size: 

                3204     773     459     209     133
 
TatI w'GTAC_w

Cuts at:    782    1047    4293     782 
   Size:        265    3246    1267

  Fragments arranged by size: 

                3246    1267     265
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4295    4327    4295 
   Size:         32    4746
 
Tth111I GACn'n_nGTC

Cuts at:    877    2575     877 
   Size:       1698    3080
 
XbaI T'CTAG_A

Cuts at:   1809    1809 
   Size:       4778
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    141     507    2273     141 
   Size:        366    1766    2646

  Fragments arranged by size: 

                2646    1766     366
 
XhoI C'TCGA_G

Cuts at:   1457    1457 
   Size:       4778
 
XmaI C'CCGG_G

Cuts at:   1175    1798    1175 
   Size:        623    4155
 
XmnI GAAnn'nnTTC

Cuts at:    881    1264     881 
   Size:        383    4395
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AloI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI    BbvCI    BciVI     BclI     BglI    BglII
     BlpI Bme1580I     BmrI     BplI     BpmI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI
  BsiHKAI    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI    CspCI     DraI     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI     FalI
     FspI    HaeII    Hin4I   HincII     KasI     KpnI     MluI     MmeI
     MscI     MslI   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PspXI
     PstI     PvuI    PvuII    RsrII     SacI     SalI     SapI     SbfI
     ScaI     SfcI     SfoI     SgfI     SmaI     SmlI     SpeI     SphI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI     AhdI     AleI     AlfI     ApaI     AscI    AvrII
     BaeI     BbsI     BcgI    BfrBI    BmgBI     BmtI     BsgI    BsiWI
     BsmI   BssHII   BstEII  BstZ17I     ClaI   DraIII    EcoRV     FseI
    FspAI  HindIII     HpaI     MfeI     NdeI     NheI     NotI     NruI
     NsiI     PacI     PasI    PshAI     PsiI   PspOMI     PsrI    SacII
    SanDI    SexAI     SfiI    SgrAI    SnaBI     SrfI     SspI     SwaI
     ZraI
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