Restriction map for pF1KB6698
##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcsI        
AflII       AgeI        AocI        Aor51HI     ApaI        
ApoI        AscI        AseI        AsnI        Asp718I     
AvrII       BalI        BbrPI       BbvCI       BciVI       
BclI        BfaI        BfrI        BlnI        BlpI        
Bpu1102I    BsaAI       BsaBI       BsaI        BsaWI       
BseAI       BsiWI       BsmBI       BsmI        Bsp1407I    
BspDI       BspEI       BspLU11I    BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstZ17I     Bsu36I      CelII       Cfr10I      
ClaI        CpoI        DdeI        DraI        DrdI        
Eco47III    Eco81I      EcoNI       EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HindIII     HpaI        
KpnI        MaeI        MamI        MfeI        MluI        
MluNI       MroI        MscI        MseI        MunI        
NaeI        NdeI        NgoMIV      NheI        NotI        
NruI        NsiI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        PshAI       
PshBI       Psp1406I    PstI        PvuI        RsrII       
SapI        SbfI        ScaI        SfcI        SfiI        
SfuI        SgrAI       SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        SwaI        
Tru9I       Tsp509I     XbaI        XhoI        


###################################
# ONE CUT ENZYMES (   45 enzymes) #
###################################

AccI          337  GTCGAC               GT^[AC][GT]AC
AflIII        143  ACGTGT               A^C[GA][CT]GT
AhdI         1249  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I         13  GTGCAC               G^TGCAC
AlwNI        1123  CAGGAGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaLI          13  GTGCAC               G^TGCAC
Asp700I       492  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI        1249  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII         749  TGCGCA               TGC^GCA
BamHI        1031  GGATCC               G^GATCC
BbeI           49  GGCGCC               GGCGC^C
BbsI         1047  GAAGAC               GAAGAC(2/6)
BglI           29  GCCAGTGCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII         248  AGATCT               A^GATCT
BpuAI        1047  GAAGAC               GAAGAC(2/6)
BsmFI         668  GGGAC                GGGAC(10/14)
BspHI         488  TCATGA               T^CATGA
BsrBI         699  GAGCGG               CCGCTC(-3/-3)
BstEII        705  GGTCACC              G^GT[ACGT]ACC
BstPI         705  GGTCACC              G^GT[ACGT]ACC
BstXI         188  CCATCCTGGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII        679  CACCTGGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I     1249  GACCTGGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI          469  GAAGAG               CTCTTC(1/4)
EcoO65I       705  GGTCACC              G^GT[ACGT]ACC
FspI          749  TGCGCA               TGC^GCA
HincII        337  GTCGAC               GT[CT]^[GA]AC
HindII        337  GTCGAC               GT[CT]^[GA]AC
KasI           49  GGCGCC               G^GCGCC
Ksp632I       469  GAAGAG               CTCTTC(1/4)
KspI         1074  CCGCGG               CCGC^GG
NarI           49  GGCGCC               GG^CGCC
NspI          959  GCATGT               [GA]CATG^[CT]
PpuMI         668  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI          488  TCATGA               T^CATGA
SacI          862  GAGCTC               GAGCT^C
SacII        1074  CCGCGG               CCGC^GG
SalI          337  GTCGAC               G^TCGAC
SfoI           49  GGCGCC               GGC^GCC
SmaI          726  CCCGGG               CCC^GGG
StuI         1175  AGGCCT               AGG^CCT
TfiI          137  GAATC                G^A[AT]TC
XmaCI         726  CCCGGG               C^CCGGG
XmaI          726  CCCGGG               C^CCGGG
XmnI          492  GAACACCTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AcyI           49  GGCGCC               G[GA]^CG[CT]C
             1279  GACGCC              
AspHI          13  GTGCAC               G[AT]GC[AT]^C
              862  GAGCTC              
AspI          220  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              340  GACTCGGTC           
BanII         541  GAGCCC               G[GA]GC[CT]^C
              862  GAGCTC              
BcgI           38  GCAACCAGATCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             1013  GCAGCTACTTCG        
BsaHI          49  GGCGCC               G[GA]^CG[CT]C
             1279  GACGCC              
BsiHKAI        13  GTGCAC               G[AT]GC[AT]^C
              862  GAGCTC              
BsmAI         113  GAGAC                GTCTC(1/5)
              386  GTCTC               
BspMI         712  ACCTGC               ACCTGC(4/8)
              746  ACCTGC              
DraII         614  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              668  GGGACCT             
EcoO109I      614  AGGCCCT              [GA]G^G[ACGT]CC[CT]
              668  GGGACCT             
HaeII          49  GGCGCC               [GA]GCGC^[CT]
              846  GGCGCT              
HgaI         1279  GACGC                GACGC(5/10)
              503  GCGTC               
Hin1I          49  GGCGCC               G[GA]^CG[CT]C
             1279  GACGCC              
NcoI          108  CCATGG               C^CATGG
              215  CCATGG              
PflFI         220  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              340  GACTCGGTC           
SexAI         680  ACCTGGT              A^CC[AT]GGT
             1250  ACCTGGT             
Tth111I       220  GACTCTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              340  GACTCGGTC           
XcmI          887  CCAAGAACATGATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              965  CCATGAAGGAGGTGG     


#########################################
# 3 OR MORE CUT ENZYMES (   80 enzymes) #
#########################################

AccII       AciI        AfaI        AluI        AlwI        
AvaI        AvaII       BanI        BbvI        BcnI        
BmyI        BpmI        BsaJI       BseRI       BsgI        
BsiEI       BsiYI       BslI        BsoBI       Bsp1286I    
BsrI        BssKI       BstF5I      BstNI       BstUI       
BstYI       Cac8I       CfoI        Cfr13I      DpnII       
DsaI        EaeI        EagI        EclXI       Eco52I      
EcoRII      EcoT14I     FauI        Fnu4HI      FokI        
HaeIII      HapII       HhaI        HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MboII       MflI        MnlI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PflMI       
PleI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       StyI        TaqI        TseI        
Tsp45I      TspRI       TthHB8I     Van91I      XhoII       
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com