Restriction map for pF1KB6698
 (Circular) (Six-base) MAPSORT of: pF1KB6698.seq  Check: 9570  from: 1  to: 4448



pF1KB6698  4448 bp

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:06  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    829     829 
   Size:       4448
 
AccI GT'mk_AC

Cuts at:    446    1486     446 
   Size:       1040    3408
 
Acc65I G'GTAC_C

Cuts at:   1464    1464 
   Size:       4448
 
AclI AA'CG_TT

Cuts at:   4288    4288 
   Size:       4448
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    345     591     772    1210    2273    2705    3452    3720     345 
   Size:        246     181     438    1063     432     747     268    1073

  Fragments arranged by size: 

                1073    1063     747     438     432     268     246     181
            
 
AflIII A'CryG_T

Cuts at:    251    1800    2904    3881     251 
   Size:       1549    1104     977     818

  Fragments arranged by size: 

                1549    1104     977     818
 
AgeI A'CCGG_T

Cuts at:   2836    2836 
   Size:       4448
 
AhdI GACnn_n'nnGTC

Cuts at:   1362    1362 
   Size:       4448
 
AleI CACnn'nnGTG

Cuts at:    643     643 
   Size:       4448
 
AlwNI CAG_nnn'CTG

Cuts at:   1236    1753    3320    1236 
   Size:        517    1567    2364

  Fragments arranged by size: 

                2364    1567     517
 
ApaLI G'TGCA_C

Cuts at:    121    3218     121 
   Size:       3097    1351
 
ApoI r'AATT_y

Cuts at:   1452    3801    4057    1452 
   Size:       2349     256    1843

  Fragments arranged by size: 

                2349    1843     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4448
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4448
 
AvaI C'yCGr_G

Cuts at:    290     834     967    1468     290 
   Size:        544     133     501    3270

  Fragments arranged by size: 

                3270     544     501     133
 
BamHI G'GATC_C

Cuts at:   1139    1473    1139 
   Size:        334    4114
 
BanI G'GyrC_C

Cuts at:    157     319     550     879     963    1464    1878    2126    2161 
   Size:        162     231     329      84     501     414     248      35

Cuts at:   2161     157 
   Size:       2444

  Fragments arranged by size: 

                2444     501     414     329     248     231     162      84
                  35
 
BanII G_rGCy'C

Cuts at:    653     974    1462    2492     653 
   Size:        321     488    1030    2609

  Fragments arranged by size: 

                2609    1030     488     321
 
BbeI G_GCGC'C

Cuts at:    161    1882    2130     161 
   Size:       1721     248    2479

  Fragments arranged by size: 

                2479    1721     248
 
BbsI GAAGACnn'nnnn_

Cuts at:   1162    1162 
   Size:       4448
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:     90     124     135     169    1110    1144      90 
   Size:         34      11      34     941      34    3394

  Fragments arranged by size: 

                3394     941      34      34      34      11
 
BciVI GTATCCnnnnn_n'

Cuts at:   2346    3107    4448    2346 
   Size:        761    1341    2346

  Fragments arranged by size: 

                2346    1341     761
 
BclI T'GATC_A

Cuts at:   1968    1968 
   Size:       4448
 
BglI GCCn_nnn'nGGC

Cuts at:    143     143 
   Size:       4448
 
BglII A'GATC_T

Cuts at:    356    1963    4000     356 
   Size:       1607    2037     804

  Fragments arranged by size: 

                2037    1607     804
 
BlpI GC'TnA_GC

Cuts at:   1560    1560 
   Size:       4448
 
Bme1580I G_kGCm'C

Cuts at:    125     553     884     968    2073    2166    3222     125 
   Size:        428     331      84    1105      93    1056    1351

  Fragments arranged by size: 

                1351    1105    1056     428     331      93      84
 
BmrI ACTGGGnnnn_n'

Cuts at:    417    1660    1741    2076    4220     417 
   Size:       1243      81     335    2144     645

  Fragments arranged by size: 

                2144    1243     645     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2815    2815 
   Size:       4448
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    259     331     481     259 
   Size:         72     150    4226

  Fragments arranged by size: 

                4226     150      72
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1624    2503    2995    3293    1624 
   Size:        879     492     298    2779

  Fragments arranged by size: 

                2779     879     492     298
 
BsaI GGTCTCn'nnnn_

Cuts at:   4152    4152 
   Size:       4448
 
BsaAI yAC'GTr

Cuts at:   1801    1801 
   Size:       4448
 
BsaBI GATnn'nnATC

Cuts at:      6    1962    3999       6 
   Size:       1956    2037     455

  Fragments arranged by size: 

                2037    1956     455
 
BsaHI Gr'CG_yC

Cuts at:    158    1388    1879    2127    2829    4325     158 
   Size:       1230     491     248     702    1496     281

  Fragments arranged by size: 

                1496    1230     702     491     281     248
 
BsaWI w'CCGG_w

Cuts at:   1637    2158    2836    3110    3257    3668    1637 
   Size:        521     678     274     147     411    2417

  Fragments arranged by size: 

                2417     678     521     411     274     147
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1432    1435    1438    1432 
   Size:          3       3    4442

  Fragments arranged by size: 

                4442       3       3
 
BseYI C'CCAG_C

Cuts at:    977    1873    2631    3208    3704     977 
   Size:        896     758     577     496    1721

  Fragments arranged by size: 

                1721     896     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    880    1126    1276     880 
   Size:        246     150    4052

  Fragments arranged by size: 

                4052     246     150
 
BsiEI CG_ry'CG

Cuts at:    104    1032    1163    1397    1990    2036    3244     104 
   Size:        928     131     234     593      46    1208    1308

  Fragments arranged by size: 

                1308    1208     928     593     234     131      46
 
BsiHKAI G_wGCw'C

Cuts at:    125     974    1462    2240    2430    3222     125 
   Size:        849     488     778     190     792    1351

  Fragments arranged by size: 

                1351     849     792     778     488     190
 
Bsp1286I G_dGCh'C

Cuts at:    125     553     653     884     968     974    1462    2073    2166 
   Size:        428     100     231      84       6     488     611      93

Cuts at:   2166    2240    2430    2492    3222     125 
   Size:         74     190      62     730    1351

  Fragments arranged by size: 

                1351     730     611     488     428     231     190     100
                  93      84      74      62       6
 
BspEI T'CCGG_A

Cuts at:   3668    3668 
   Size:       4448
 
BspHI T'CATG_A

Cuts at:    596    3624     596 
   Size:       3028    1420
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    829     863    1498    1712    2014    2395     829 
   Size:         34     635     214     302     381    2882

  Fragments arranged by size: 

                2882     635     381     302     214      34
 
BsrBI CCG'CTC

Cuts at:     50     809    2740    2794    4278    4443      50 
   Size:        759    1931      54    1484     165      55

  Fragments arranged by size: 

                1931    1484     759     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2360    2360 
   Size:       4448
 
BsrFI r'CCGG_y

Cuts at:   2446    2627    2836    2446 
   Size:        181     209    4058

  Fragments arranged by size: 

                4058     209     181
 
BssSI C'ACGA_G

Cuts at:   2719    3077    2719 
   Size:        358    4090
 
BstAPI GCAn_nnn'nTGC

Cuts at:   1825    1825 
   Size:       4448
 
BstBI TT'CG_AA

Cuts at:   2809    2809 
   Size:       4448
 
BstEII G'GTnAC_C

Cuts at:    813     813 
   Size:       4448
 
BstXI CCAn_nnnn'nTGG

Cuts at:    303     303 
   Size:       4448
 
BstYI r'GATC_y

Cuts at:      1     306     356     569    1139    1473    1940    1963    2298 
   Size:        305      50     213     570     334     467      23     335

Cuts at:   2298    2544    3556    3642    3654    4000       1 
   Size:        246    1012      86      12     346     449

  Fragments arranged by size: 

                1012     570     467     449     346     335     334     305
                 246     213      86      50      23      12
 
BtgI C'CryG_G

Cuts at:    216     323    1026    1182    1391     216 
   Size:        107     703     156     209    3273

  Fragments arranged by size: 

                3273     703     209     156     107
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    197    2398    2579    2742     197 
   Size:       2201     181     163    1903

  Fragments arranged by size: 

                2201    1903     181     163
 
BtsI GCAGTG_nn'

Cuts at:   1691    1691 
   Size:       4448
 
DraI TTT'AAA

Cuts at:   1447    1447 
   Size:       4448
 
DraIII CAC_nnn'GTG

Cuts at:    792     792 
   Size:       4448
 
DrdI GACnn_nn'nnGTC

Cuts at:   2154    3012    2154 
   Size:        858    3590
 
EaeI y'GGCC_r

Cuts at:    351     384     797    1007    1029    1160    1199    1223    1265 
   Size:         33     413     210      22     131      39      24      42

Cuts at:   1265    1394    2033    2207    2598    2625    3970     351 
   Size:        129     639     174     391      27    1345     829

  Fragments arranged by size: 

                1345     829     639     413     391     210     174     131
                 129      42      39      33      27      24      22
 
EagI C'GGCC_G

Cuts at:   1029    1160    1394    2033    1029 
   Size:        131     234     639    3444

  Fragments arranged by size: 

                3444     639     234     131
 
EarI CTCTTCn'nnn_

Cuts at:    572    2471    2681     572 
   Size:       1899     210    2339

  Fragments arranged by size: 

                2339    1899     210
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2656    2966    3112    4258    2656 
   Size:        310     146    1146    2846

  Fragments arranged by size: 

                2846    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:    972    1460     972 
   Size:        488    3960
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    259     331     345     481     591     772    1210    2273    2705 
   Size:         72      14     136     110     181     438    1063     432

Cuts at:   2705    3452    3720     259 
   Size:        747     268     987

  Fragments arranged by size: 

                1063     987     747     438     432     268     181     136
                 110      72      14
 
EcoO109I rG'GnC_Cy

Cuts at:    723     777    1587     723 
   Size:         54     810    3584

  Fragments arranged by size: 

                3584     810      54
 
EcoRI G'AATT_C

Cuts at:   1452    1452 
   Size:       4448
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   2467    2467 
   Size:       4448
 
FspI TGC'GCA

Cuts at:    859    2229     859 
   Size:       1370    3078
 
HaeII r_GCGC'y

Cuts at:    161     958    1882    2130    3152    4131     161 
   Size:        797     924     248    1022     979     478

  Fragments arranged by size: 

                1022     979     924     797     478     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    504     536    1348    1380    2290    2320    2322    2352     504 
   Size:         32     812      32     910      30       2      30    2600

  Fragments arranged by size: 

                2600     910     812      32      32      30      30       2
            
 
HincII GTy'rAC

Cuts at:    447    1487     447 
   Size:       1040    3408
 
KasI G'GCGC_C

Cuts at:    157    1878    2126     157 
   Size:       1721     248    2479

  Fragments arranged by size: 

                2479    1721     248
 
KpnI G_GTAC'C

Cuts at:   1468    1468 
   Size:       4448
 
MluI A'CGCG_T

Cuts at:   3881    3881 
   Size:       4448
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   3119    3303    3119 
   Size:        184    4264
 
MscI TGG'CCA

Cuts at:   2209    2209 
   Size:       4448
 
MslI CAynn'nnrTG

Cuts at:    301     643    1072    1267    2437    2564     301 
   Size:        342     429     195    1170     127    2185

  Fragments arranged by size: 

                2185    1170     429     342     195     127
 
MspA1I CmG'CkG

Cuts at:    237     510     681     843    1184    1559    1873    2233    3246 
   Size:        273     171     162     341     375     314     360    1013

Cuts at:   3246    3491     237 
   Size:        245    1194

  Fragments arranged by size: 

                1194    1013     375     360     341     314     273     245
                 171     162
 
NaeI GCC'GGC

Cuts at:   2629    2629 
   Size:       4448
 
NarI GG'CG_CC

Cuts at:    158    1879    2127     158 
   Size:       1721     248    2479

  Fragments arranged by size: 

                2479    1721     248
 
NcoI C'CATG_G

Cuts at:    216     323     216 
   Size:        107    4341
 
NgoMIV G'CCGG_C

Cuts at:   2627    2627 
   Size:       4448
 
NspI r_CATG'y

Cuts at:   1071    1501    2001    2908    1071 
   Size:        430     500     907    2611

  Fragments arranged by size: 

                2611     907     500     430
 
PciI A'CATG_T

Cuts at:   2904    2904 
   Size:       4448
 
PflMI CCAn_nnn'nTGG

Cuts at:    167     522     681     684     167 
   Size:        355     159       3    3931

  Fragments arranged by size: 

                3931     355     159       3
 
PfoI T'CCnGG_A

Cuts at:    455     541     572    1229     455 
   Size:         86      31     657    3674

  Fragments arranged by size: 

                3674     657      86      31
 
PmeI GTTT'AAAC

Cuts at:   1447    1447 
   Size:       4448
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   1312    1344    3587    3619    1312 
   Size:         32    2243      32    2141

  Fragments arranged by size: 

                2243    2141      32      32
 
PpuMI rG'GwC_Cy

Cuts at:    777     777 
   Size:       4448
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:   1108    1140    1108 
   Size:         32    4416
 
PstI C_TGCA'G

Cuts at:   1495    2180    1495 
   Size:        685    3763
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4448
 
PvuII CAG'CTG

Cuts at:    237     510     681     843    1873    2233     237 
   Size:        273     171     162    1030     360    2452

  Fragments arranged by size: 

                2452    1030     360     273     171     162
 
RsrII CG'GwC_CG

Cuts at:   2643    2643 
   Size:       4448
 
SacI G_AGCT'C

Cuts at:    974    1462     974 
   Size:        488    3960
 
SacII CC_GC'GG

Cuts at:   1185    1185 
   Size:       4448
 
SalI G'TCGA_C

Cuts at:    445    1485     445 
   Size:       1040    3408
 
SapI GCTCTTCn'nnn_

Cuts at:   2471    2681    2471 
   Size:        210    4238
 
SbfI CC_TGCA'GG

Cuts at:   1495    1495 
   Size:       4448
 
ScaI AGT'ACT

Cuts at:   3965    3965 
   Size:       4448
 
SexAI A'CCwGG_T

Cuts at:    788    1358     788 
   Size:        570    3878
 
SfcI C'TryA_G

Cuts at:     33    1491    2176    3169    3360      33 
   Size:       1458     685     993     191    1121

  Fragments arranged by size: 

                1458    1121     993     685     191
 
SfoI GGC'GCC

Cuts at:    159    1880    2128     159 
   Size:       1721     248    2479

  Fragments arranged by size: 

                2479    1721     248
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4448
 
SmaI CCC'GGG

Cuts at:    836    1470     836 
   Size:        634    3814
 
SmlI C'TyrA_G

Cuts at:   1603    2518    3010    3272    1603 
   Size:        915     492     262    2779

  Fragments arranged by size: 

                2779     915     492     262
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4448
 
SphI G_CATG'C

Cuts at:   1501    2001    1501 
   Size:        500    3948
 
StuI AGG'CCT

Cuts at:   1285    1285 
   Size:       4448
 
StyI C'CwwG_G

Cuts at:    216     323     413     626    1582    1936     216 
   Size:        107      90     213     956     354    2728

  Fragments arranged by size: 

                2728     956     354     213     107      90
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    441     639     660     991    2031    2164    2623    2832     441 
   Size:        198      21     331    1040     133     459     209    2057

  Fragments arranged by size: 

                2057    1040     459     331     209     198     133      21
            
 
TatI w'GTAC_w

Cuts at:    254    3963     254 
   Size:       3709     739
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   3965    3997    3965 
   Size:         32    4416
 
Tth111I GACn'n_nGTC

Cuts at:    331     451    2245     331 
   Size:        120    1794    2534

  Fragments arranged by size: 

                2534    1794     120
 
XbaI T'CTAG_A

Cuts at:   1479    1479 
   Size:       4448
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   1002    1080    1943    1002 
   Size:         78     863    3507

  Fragments arranged by size: 

                3507     863      78
 
XmaI C'CCGG_G

Cuts at:    834    1468     834 
   Size:        634    3814
 
XmnI GAAnn'nnTTC

Cuts at:    604     604 
   Size:       4448
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI     AhdI
     AleI    AlwNI    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI
     BanI    BanII     BbeI     BbsI     BcgI    BciVI     BclI     BglI
    BglII     BlpI Bme1580I     BmrI     BplI     BpmI    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EagI     EarI     EciI
  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII    Hin4I
   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI     PfoI
     PmeI     PpiI    PpuMI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI     SapI     SbfI     ScaI    SexAI     SfcI
     SfoI     SgfI     SmaI     SmlI     SpeI     SphI     StuI     StyI
    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AlfI     AloI     ApaI     AscI    AvrII
     BaeI    BbvCI    BfrBI    BmgBI     BmtI   Bpu10I    BsaXI    BsiWI
     BsmI    BsmBI    BsrGI   BssHII  BstZ17I   Bsu36I     ClaI    CspCI
    EcoNI    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI
     NheI     NotI     NruI     NsiI     PacI     PasI     PmlI    PshAI
     PsiI   PspOMI    PspXI    SanDI     SfiI    SgrAI    SnaBI     SrfI
     SspI     SwaI     XhoI     ZraI
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