Restriction map for pF1KB0498
##################################
# NO CUT ENZYMES (  132 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      ApaLI       ApoI        
AscI        Asp718I     AspEI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BbvCI       BciVI       BclI        BfrI        
BglI        BlnI        BlpI        Bpu1102I    BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BsgI        
BsiWI       BsmBI       BsoBI       Bsp1407I    BspDI       
BspEI       BspLU11I    BsrDI       BsrGI       BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstUI       
BstXI       BstZ17I     CelII       ClaI        CpoI        
DraI        DraIII      DrdI        EagI        Eam1105I    
EclXI       Eco52I      EcoNI       EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
Hin1I       HpaI        KasI        KpnI        KspI        
MamI        MfeI        MluI        MroI        MunI        
MvnI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PmaCI       PmeI        PmlI        
PshAI       Psp1406I    PvuI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        SwaI        Tth111I     Van91I      XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   44 enzymes) #
###################################

AlwNI         133  CAGAGGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI      1174  AGCGCT               AGC^GCT
ApaI          141  GGGCCC               GGGCC^C
AspHI         732  GAGCTC               G[AT]GC[AT]^C
BalI          221  TGGCCA               TGG^CCA
BbsI          976  GAAGAC               GAAGAC(2/6)
BcgI          822  GCAGCAGGTTCG         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI          804  CCGGG                CC^[GC]GG
BfaI         1040  CTAG                 C^TAG
BglII         582  AGATCT               A^GATCT
BpmI          925  CTGGAG               CTGGAG(16/14)
BpuAI         976  GAAGAC               GAAGAC(2/6)
BsaAI         310  TACGTG               [CT]AC^GT[GA]
BseRI         601  GAGGAG               GAGGAG(10/8)
BsiEI         625  CGACCG               CG[GA][CT]^CG
BsiHKAI       732  GAGCTC               G[AT]GC[AT]^C
BsmI          477  GAATGC               GAATGC(1/-1)
BspHI         360  TCATGA               T^CATGA
BsrBI         808  GAGCGG               CCGCTC(-3/-3)
BsrFI         699  ACCGGC               [GA]^CCGG[CT]
Cfr10I        699  ACCGGC               [GA]^CCGG[CT]
DsaI          743  CCGTGG               C^C[GA][CT]GG
EaeI          221  TGGCCA               [CT]^GGCC[GA]
Eco47III     1174  AGCGCT               AGC^GCT
EcoT14I       401  CCAAGG               C^C[AT][AT]GG
HgaI          888  GACGC                GACGC(5/10)
HincII        286  GTTGAC               GT[CT]^[GA]AC
HindII        286  GTTGAC               GT[CT]^[GA]AC
HindIII       529  AAGCTT               A^AGCTT
MaeI         1040  CTAG                 C^TAG
MluNI         221  TGGCCA               TGG^CCA
MscI          221  TGGCCA               TGG^CCA
MslI          361  CATGAAGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI          804  CCGGG                CC^[GC]GG
PpuMI        1157  GGGACCT              [GA]G^G[AT]CC[CT]
RcaI          360  TCATGA               T^CATGA
SacI          732  GAGCTC               GAGCT^C
SapI         1215  GAAGAGC              GCTCTTC(1/4)
SfaNI        1009  GATGC                GCATC(5/9)
StyI          401  CCAAGG               C^C[AT][AT]GG
TaqI          831  TCGA                 T^CGA
TfiI         1204  GAATC                G^A[AT]TC
TthHB8I       831  TCGA                 T^CGA
XbaI         1039  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   26 enzymes) #
###################################

AfaI           90  GTAC                 GT^AC
              105  GTAC                
AlwI         1246  GATCC                GGATC(4/5)
             1150  GGATC               
AocI          126  CCTGAGG              CC^T[ACGT]AGG
              462  CCTCAGG             
AseI          214  ATTAAT               AT^TAAT
              960  ATTAAT              
AsnI          214  ATTAAT               AT^TAAT
              960  ATTAAT              
Asp700I        16  GAAGCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              930  GAAAGACTTC          
BspMI         660  GCAGGT               ACCTGC(4/8)
              825  GCAGGT              
BstEII        663  GGTGACC              G^GT[ACGT]ACC
             1092  GGTGACC             
BstPI         663  GGTGACC              G^GT[ACGT]ACC
             1092  GGTGACC             
BstYI         582  AGATCT               [GA]^GATC[CT]
             1150  GGATCT              
Bsu36I        126  CCTGAGG              CC^T[ACGT]AGG
              462  CCTCAGG             
CfoI          868  GCGC                 GCG^C
             1175  GCGC                
Eco81I        126  CCTGAGG              CC^T[ACGT]AGG
              462  CCTCAGG             
EcoO65I       663  GGTGACC              G^GT[ACGT]ACC
             1092  GGTGACC             
HaeII         867  AGCGCC               [GA]GCGC^[CT]
             1174  AGCGCT              
HhaI          868  GCGC                 GCG^C
             1175  GCGC                
HinP1I        868  GCGC                 G^CGC
             1175  GCGC                
MaeII          33  ACGT                 A^CGT
              311  ACGT                
MflI          582  AGATCT               [GA]^GATC[CT]
             1150  GGATCT              
PshBI         214  ATTAAT               AT^TAAT
              960  ATTAAT              
RsaI           90  GTAC                 GT^AC
              105  GTAC                
SmlI          394  CTCAAG               C^T[CT][GA]AG
              953  CTCAAG              
TspRI          65  CAGTG                CA[GC]TG[ACGT][ACGT]^
               82  CAGTG               
XcmI           42  CCATCCTGCTGCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              339  CCAGCTGAAGGATGG     
XhoII         582  AGATCT               [GA]^GATC[CT]
             1150  GGATCT              
XmnI           16  GAAGCAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              930  GAAAGACTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   56 enzymes) #
#########################################

AciI        AluI        AvaII       BanII       BbvI        
BmyI        BsaJI       BsiYI       BslI        BsmAI       
BsmFI       Bsp1286I    BsrI        BssKI       BstF5I      
BstNI       Cac8I       Cfr13I      DdeI        DpnII       
DraII       EarI        EcoO109I    EcoRII      Fnu4HI      
FokI        HaeIII      HapII       HinfI       HpaII       
HphI        ItaI        Ksp632I     MaeIII      MboI        
MboII       MnlI        MseI        MspA1I      MspI        
MvaI        MwoI        NdeII       NlaIII      NlaIV       
PleI        PstI        PvuII       Sau3AI      Sau96I      
ScrFI       SfcI        Tru9I       TseI        Tsp45I      
Tsp509I     
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