Restriction map for pF1KB0498
 (Linear) (Six-base) MAPSORT of: KIBB0498.seq  Check: 7266  from: 1  to: 1254



KIBB0498.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     651    1254 
   Size:        651     603
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     472    1254 
   Size:        364     108     782

  Fragments arranged by size: 

                 782     364     108
 
AfeI AGC'GCT

Cuts at:      0    1176    1254 
   Size:       1176      78
 
AlwNI CAG_nnn'CTG

Cuts at:      0     138    1254 
   Size:        138    1116
 
ApaI G_GGCC'C

Cuts at:      0     145    1254 
   Size:        145    1109
 
AseI AT'TA_AT

Cuts at:      0     215     961    1254 
   Size:        215     746     293

  Fragments arranged by size: 

                 746     293     215
 
BanII G_rGCy'C

Cuts at:      0     145     462     736    1254 
   Size:        145     317     274     518

  Fragments arranged by size: 

                 518     317     274     145
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     983    1254 
   Size:        983     271
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     811     845    1254 
   Size:        811      34     409

  Fragments arranged by size: 

                 811     409      34
 
BglII A'GATC_T

Cuts at:      0     582    1254 
   Size:        582     672
 
Bme1580I G_kGCm'C

Cuts at:      0     145     255    1254 
   Size:        145     110     999

  Fragments arranged by size: 

                 999     145     110
 
BmgBI CAC'GTC

Cuts at:      0      34    1254 
   Size:         34    1220
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1209    1254 
   Size:       1209      45
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     946    1254 
   Size:        946     308
 
Bpu10I CC'TnA_GC

Cuts at:      0     730    1254 
   Size:        730     524
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     379     938    1254 
   Size:        379     559     316

  Fragments arranged by size: 

                 559     379     316
 
BsaAI yAC'GTr

Cuts at:      0     312    1254 
   Size:        312     942
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     703     733    1254 
   Size:        703      30     521

  Fragments arranged by size: 

                 703     521      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     616    1254 
   Size:        616     638
 
BseYI C'CCAG_C

Cuts at:      0     138     248     682    1050    1254 
   Size:        138     110     434     368     204

  Fragments arranged by size: 

                 434     368     204     138     110
 
BsiEI CG_ry'CG

Cuts at:      0     628    1254 
   Size:        628     626
 
BsiHKAI G_wGCw'C

Cuts at:      0     736    1254 
   Size:        736     518
 
BsmI GAATG_Cn'

Cuts at:      0     483    1254 
   Size:        483     771
 
Bsp1286I G_dGCh'C

Cuts at:      0     145     255     462     736    1254 
   Size:        145     110     207     274     518

  Fragments arranged by size: 

                 518     274     207     145     110
 
BspHI T'CATG_A

Cuts at:      0     360    1254 
   Size:        360     894
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     651     816    1254 
   Size:        651     165     438

  Fragments arranged by size: 

                 651     438     165
 
BsrBI CCG'CTC

Cuts at:      0     810    1254 
   Size:        810     444
 
BsrFI r'CCGG_y

Cuts at:      0     699    1254 
   Size:        699     555
 
BstEII G'GTnAC_C

Cuts at:      0     663    1092    1254 
   Size:        663     429     162

  Fragments arranged by size: 

                 663     429     162
 
BstYI r'GATC_y

Cuts at:      0     582    1150    1254 
   Size:        582     568     104

  Fragments arranged by size: 

                 582     568     104
 
Bsu36I CC'TnA_GG

Cuts at:      0     127     463    1254 
   Size:        127     336     791

  Fragments arranged by size: 

                 791     336     127
 
BtgI C'CryG_G

Cuts at:      0     743    1254 
   Size:        743     511
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     524     559    1254 
   Size:        524      35     695

  Fragments arranged by size: 

                 695     524      35
 
EaeI y'GGCC_r

Cuts at:      0     221    1254 
   Size:        221    1033
 
EarI CTCTTCn'nnn_

Cuts at:      0      95     568     850    1210    1254 
   Size:         95     473     282     360      44

  Fragments arranged by size: 

                 473     360     282      95      44
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     188     718     729    1254 
   Size:        188     530      11     525

  Fragments arranged by size: 

                 530     525     188      11
 
EcoICRI GAG'CTC

Cuts at:      0     734    1254 
   Size:        734     520
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     364     472     946    1254 
   Size:        364     108     474     308

  Fragments arranged by size: 

                 474     364     308     108
 
EcoO109I rG'GnC_Cy

Cuts at:      0     123     390    1158    1254 
   Size:        123     267     768      96

  Fragments arranged by size: 

                 768     267     123      96
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      40     955    1254 
   Size:         40     915     299

  Fragments arranged by size: 

                 915     299      40
 
HaeII r_GCGC'y

Cuts at:      0     871    1178    1254 
   Size:        871     307      76

  Fragments arranged by size: 

                 871     307      76
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     567     599     703     735    1254 
   Size:        567      32     104      32     519

  Fragments arranged by size: 

                 567     519     104      32      32
 
HincII GTy'rAC

Cuts at:      0     288    1254 
   Size:        288     966
 
HindIII A'AGCT_T

Cuts at:      0     529    1254 
   Size:        529     725
 
MscI TGG'CCA

Cuts at:      0     223    1254 
   Size:        223    1031
 
MslI CAynn'nnrTG

Cuts at:      0     365    1254 
   Size:        365     889
 
MspA1I CmG'CkG

Cuts at:      0     342     620     654     768     789     850    1254 
   Size:        342     278      34     114      21      61     404

  Fragments arranged by size: 

                 404     342     278     114      61      34      21
 
PasI CC'CwG_GG

Cuts at:      0     639    1254 
   Size:        639     615
 
PfoI T'CCnGG_A

Cuts at:      0     803     923    1254 
   Size:        803     120     331

  Fragments arranged by size: 

                 803     331     120
 
PpuMI rG'GwC_Cy

Cuts at:      0    1158    1254 
   Size:       1158      96
 
PspOMI G'GGCC_C

Cuts at:      0     141    1254 
   Size:        141    1113
 
PstI C_TGCA'G

Cuts at:      0     659     718     794    1254 
   Size:        659      59      76     460

  Fragments arranged by size: 

                 659     460      76      59
 
PvuII CAG'CTG

Cuts at:      0     342     620     654     789     850    1254 
   Size:        342     278      34     135      61     404

  Fragments arranged by size: 

                 404     342     278     135      61      34
 
SacI G_AGCT'C

Cuts at:      0     736    1254 
   Size:        736     518
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1210    1254 
   Size:       1210      44
 
SfcI C'TryA_G

Cuts at:      0     655     714     790    1254 
   Size:        655      59      76     464

  Fragments arranged by size: 

                 655     464      76      59
 
SmlI C'TyrA_G

Cuts at:      0     394     953    1254 
   Size:        394     559     301

  Fragments arranged by size: 

                 559     394     301
 
StyI C'CwwG_G

Cuts at:      0     401    1254 
   Size:        401     853
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     642     662    1254 
   Size:        642      20     592

  Fragments arranged by size: 

                 642     592      20
 
TatI w'GTAC_w

Cuts at:      0      89    1254 
   Size:         89    1165
 
XbaI T'CTAG_A

Cuts at:      0    1039    1254 
   Size:       1039     215
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      49     346    1254 
   Size:         49     297     908

  Fragments arranged by size: 

                 908     297      49
 
XmnI GAAnn'nnTTC

Cuts at:      0      20     934    1254 
   Size:         20     914     320

  Fragments arranged by size: 

                 914     320      20
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AlwNI     ApaI     AseI    BanII     BbsI
     BcgI    BglII Bme1580I    BmgBI     BmrI     BpmI   Bpu10I    BpuEI
    BsaAI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI     BsmI Bsp1286I
    BspHI    BspMI    BsrBI    BsrFI   BstEII    BstYI   Bsu36I     BtgI
    CspCI     EaeI     EarI     EciI  EcoICRI  Eco57MI EcoO109I     FalI
    HaeII    Hin4I   HincII  HindIII     MscI     MslI   MspA1I     PasI
     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI     SapI     SfcI
     SmlI     StyI    TaqII     TatI     XbaI     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     ApoI     AscI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI    BciVI     BclI
    BfrBI     BglI     BlpI     BmtI     BplI     BsaI    BsaBI    BsaHI
    BsaWI     BsgI    BsiWI    BsmBI    BspEI    BsrDI    BsrGI   BssHII
    BssSI   BstAPI    BstBI    BstXI  BstZ17I    BtgZI     BtsI     ClaI
     DraI   DraIII     DrdI     EagI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PciI    PflMI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
     TstI  Tth111I     XhoI     XmaI     ZraI
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