Restriction map for pF1KB5352
##################################
# NO CUT ENZYMES (  147 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AcyI        
AflII       AgeI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp718I     AspHI       AspI        AvaI        
AviII       AvrII       BamHI       BanI        BbeI        
BbrPI       BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BlnI        BlpI        Bpu1102I    
BsaAI       BsaHI       BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmFI       
BsmI        BsoBI       Bsp1407I    BspDI       BspEI       
BspHI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       BstAPI      BstEII      BstPI       
BstXI       Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DraI        DraII       DraIII      DrdI        
EagI        EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FauI        FbaI        FseI        FspI        
HaeII       HapII       HgaI        Hin1I       HindIII     
HpaII       KasI        KpnI        KspI        MluI        
MroI        MspA1I      MspI        MwoI        NaeI        
NarI        NciI        NdeI        NgoMIV      NheI        
NotI        NsiI        PacI        PaeR7I      PflFI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsrII       SacI        SacII       
SalI        SapI        SbfI        ScaI        SexAI       
SfiI        SfoI        SgrAI       SmaI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       StuI        
SwaI        Tsp45I      Tth111I     XbaI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AflIII       1061  ACATGT               A^C[GA][CT]GT
Asp700I       960  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaII         126  GGACC                G^G[AT]CC
BalI         1253  TGGCCA               TGG^CCA
BanII         920  GGGCTC               G[GA]GC[CT]^C
BbsI          559  GAAGAC               GAAGAC(2/6)
BbvCI         933  GCTGAGG              CCTCAGC(-5/-2)
BglII         825  AGATCT               A^GATCT
BmyI          920  GGGCTC               G[AGT]GC[ACT]^C
BpmI         1088  CTCCAG               CTGGAG(16/14)
BpuAI         559  GAAGAC               GAAGAC(2/6)
BsaBI          43  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiYI        1245  CCAGATGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI         1245  CCAGATGTTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI        1009  CGTCTC               CGTCTC(1/5)
Bsp1286I      920  GGGCTC               G[AGT]GC[ACT]^C
BspLU11I     1061  ACATGT               A^CATGT
Bst1107I      169  GTATAC               GTA^TAC
BstBI         800  TTCGAA               TT^CGAA
BstZ17I       169  GTATAC               GTA^TAC
Cac8I         516  GCTGGC               GC[ACGT]^[ACGT]GC
CfoI           31  GCGC                 GCG^C
Cfr13I        126  GGACC                G^G[ACGT]CC
DsaI          554  CCATGG               C^C[GA][CT]GG
EaeI         1253  TGGCCA               [CT]^GGCC[GA]
EcoT14I       554  CCATGG               C^C[AT][AT]GG
HhaI           31  GCGC                 GCG^C
HinP1I         31  GCGC                 G^CGC
HpaI          901  GTTAAC               GTT^AAC
MaeII         807  ACGT                 A^CGT
MaeIII       1181  GTAAC                ^GT[ACGT]AC
MamI           43  GATCGGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI          269  CAATTG               C^AATTG
MluNI        1253  TGGCCA               TGG^CCA
MscI         1253  TGGCCA               TGG^CCA
MslI         1056  CATTAACATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI          269  CAATTG               C^AATTG
NcoI          554  CCATGG               C^CATGG
NruI          684  TCGCGA               TCG^CGA
NspI         1061  ACATGT               [GA]CATG^[CT]
NspV          800  TTCGAA               TT^CGAA
PflMI        1245  CCAGATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau96I        126  GGACC                G^G[ACGT]CC
SfuI          800  TTCGAA               TT^CGAA
StyI          554  CCATGG               C^C[AT][AT]GG
TaqI          801  TCGA                 T^CGA
TthHB8I       801  TCGA                 T^CGA
Van91I       1245  CCAGATGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI          411  CCAGTACGAATGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI          960  GAATGACTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   35 enzymes) #
###################################

AccI          151  GTAGAC               GT^[AC][GT]AC
              169  GTATAC              
AccII          30  CGCG                 CG^CG
              685  CGCG                
AfaI            9  GTAC                 GT^AC
              414  GTAC                
AhdI          741  GACAATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1026  GACTTTCTGTC         
AlwI          289  GATCC                GGATC(4/5)
               42  GGATC               
AspEI         741  GACAATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1026  GACTTTCTGTC         
BbvI          439  GCAGC                GCAGC(8/12)
              550  GCAGC               
BfaI           52  CTAG                 C^TAG
              829  CTAG                
BsaI          580  GAGACC               GGTCTC(1/5)
              650  GGTCTC              
BsaJI         320  CCAGGG               C^C[ACGT][ACGT]GG
              554  CCATGG              
BspMI         813  GCAGGT               ACCTGC(4/8)
             1289  GCAGGT              
BssKI         320  CCAGG                ^CC[ACGT]GG
              348  CCTGG               
BstNI         320  CCAGG                CC^[AT]GG
              348  CCTGG               
BstUI          30  CGCG                 CG^CG
              685  CGCG                
BstYI         288  AGATCC               [GA]^GATC[CT]
              825  AGATCT              
Eam1105I      741  GACAATGTGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
             1026  GACTTTCTGTC         
EcoRII        320  CCAGG                ^CC[AT]GG
              348  CCTGG               
Fnu4HI        439  GCAGC                GC^[ACGT]GC
              550  GCAGC               
HaeIII        519  GGCC                 GG^CC
             1254  GGCC                
HincII        315  GTCAAC               GT[CT]^[GA]AC
              901  GTTAAC              
HindII        315  GTCAAC               GT[CT]^[GA]AC
              901  GTTAAC              
HphI          672  TCACC                GGTGA(8/7)
             1195  TCACC               
ItaI          439  GCAGC                GC^[ACGT]GC
              550  GCAGC               
MaeI           52  CTAG                 C^TAG
              829  CTAG                
MflI          288  AGATCC               [GA]^GATC[CT]
              825  AGATCT              
MvaI          320  CCAGG                CC^[AT]GG
              348  CCTGG               
MvnI           30  CGCG                 CG^CG
              685  CGCG                
NlaIV         471  GGTTCC               GG[ACGT]^[ACGT]CC
             1299  GGCTCC              
PleI         1086  GACTC                GAGTC(4/5)
              850  GAGTC               
RsaI            9  GTAC                 GT^AC
              414  GTAC                
ScrFI         320  CCAGG                CC^[ACGT]GG
              348  CCTGG               
SfcI          393  CTACAG               C^T[GA][CT]AG
              773  CTACAG              
SmlI          985  CTTGAG               C^T[CT][GA]AG
             1316  CTCAAG              
TseI          439  GCAGC                G^C[AT]GC
              550  GCAGC               
XhoII         288  AGATCC               [GA]^GATC[CT]
              825  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   26 enzymes) #
#########################################

AciI        AcsI        AluI        ApoI        BsmAI       
BsrI        BstF5I      DdeI        DpnII       EarI        
FokI        HinfI       Ksp632I     MboI        MboII       
MnlI        MseI        NdeII       NlaIII      Sau3AI      
SfaNI       SspI        TfiI        Tru9I       Tsp509I     
TspRI       
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