Restriction map for pF1KB5427
 (Circular) (Six-base) MAPSORT of: pF1KB5427.seq  Check: 965  from: 1  to: 4247



pF1KB5427  4247 bp

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:    666     666 
   Size:       4247
 
AccI GT'mk_AC

Cuts at:   1285    1285 
   Size:       4247
 
Acc65I G'GTAC_C

Cuts at:   1263    1263 
   Size:       4247
 
AclI AA'CG_TT

Cuts at:   4087    4087 
   Size:       4247
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:    148     920    2072    2504    3251    3519     148 
   Size:        772    1152     432     747     268     876

  Fragments arranged by size: 

                1152     876     772     747     432     268
 
AflIII A'CryG_T

Cuts at:   1599    2703    3680    1599 
   Size:       1104     977    2166

  Fragments arranged by size: 

                2166    1104     977
 
AgeI A'CCGG_T

Cuts at:   2635    2635 
   Size:       4247
 
AlwNI CAG_nnn'CTG

Cuts at:    141    1083    1233    1552    3119     141 
   Size:        942     150     319    1567    1269

  Fragments arranged by size: 

                1567    1269     942     319     150
 
ApaLI G'TGCA_C

Cuts at:   3017    3017 
   Size:       4247
 
ApoI r'AATT_y

Cuts at:   1251    3600    3856    1251 
   Size:       2349     256    1642

  Fragments arranged by size: 

                2349    1642     256
 
AseI AT'TA_AT

Cuts at:     20      20 
   Size:       4247
 
AsiSI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4247
 
AvaI C'yCGr_G

Cuts at:    604     683    1267     604 
   Size:         79     584    3584

  Fragments arranged by size: 

                3584     584      79
 
BamHI G'GATC_C

Cuts at:    878    1272     878 
   Size:        394    3853
 
BanI G'GyrC_C

Cuts at:    982    1005    1263    1677    1925    1960     982 
   Size:         23     258     414     248      35    3269

  Fragments arranged by size: 

                3269     414     258     248      35      23
 
BanII G_rGCy'C

Cuts at:    460    1201    1261    2291     460 
   Size:        741      60    1030    2416

  Fragments arranged by size: 

                2416    1030     741      60
 
BbeI G_GCGC'C

Cuts at:   1681    1929    1681 
   Size:        248    3999
 
BbsI GAAGACnn'nnnn_

Cuts at:    147     525     896     908    1100    1131     147 
   Size:        378     371      12     192      31    3263

  Fragments arranged by size: 

                3263     378     371     192      31      12
 
BciVI GTATCCnnnnn_n'

Cuts at:   2145    2906    4247    2145 
   Size:        761    1341    2145

  Fragments arranged by size: 

                2145    1341     761
 
BclI T'GATC_A

Cuts at:   1094    1767    1094 
   Size:        673    3574
 
BglI GCCn_nnn'nGGC

Cuts at:   1014    1014 
   Size:       4247
 
BglII A'GATC_T

Cuts at:   1762    3799    1762 
   Size:       2037    2210
 
BlpI GC'TnA_GC

Cuts at:   1359    1359 
   Size:       4247
 
Bme1580I G_kGCm'C

Cuts at:    319     640     985    1010    1872    1965    3021     319 
   Size:        321     345      25     862      93    1056    1545

  Fragments arranged by size: 

                1545    1056     862     345     321      93      25
 
BmrI ACTGGGnnnn_n'

Cuts at:   1459    1540    1875    4019    1459 
   Size:         81     335    2144    1687

  Fragments arranged by size: 

                2144    1687     335      81
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2614    2614 
   Size:       4247
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:    322     444     322 
   Size:        122    4125
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:    264     340     691     780    1423    2302    2794    3092     264 
   Size:         76     351      89     643     879     492     298    1419

  Fragments arranged by size: 

                1419     879     643     492     351     298      89      76
            
 
BsaI GGTCTCn'nnnn_

Cuts at:   3951    3951 
   Size:       4247
 
BsaAI yAC'GTr

Cuts at:   1600    1600 
   Size:       4247
 
BsaBI GATnn'nnATC

Cuts at:      6    1761    3798       6 
   Size:       1755    2037     455

  Fragments arranged by size: 

                2037    1755     455
 
BsaHI Gr'CG_yC

Cuts at:    997    1678    1926    2628    4124     997 
   Size:        681     248     702    1496    1120

  Fragments arranged by size: 

                1496    1120     702     681     248
 
BsaWI w'CCGG_w

Cuts at:    245    1436    1957    2635    2909    3056    3467     245 
   Size:       1191     521     678     274     147     411    1025

  Fragments arranged by size: 

                1191    1025     678     521     411     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:    295     325     295 
   Size:         30    4217
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:    205     208     768     979     205 
   Size:          3     560     211    3473

  Fragments arranged by size: 

                3473     560     211       3
 
BseYI C'CCAG_C

Cuts at:    828    1672    2430    3007    3503     828 
   Size:        844     758     577     496    1572

  Fragments arranged by size: 

                1572     844     758     577     496
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:    913     913 
   Size:       4247
 
BsiEI CG_ry'CG

Cuts at:    104    1789    1835    3043     104 
   Size:       1685      46    1208    1308

  Fragments arranged by size: 

                1685    1308    1208      46
 
BsiHKAI G_wGCw'C

Cuts at:    919    1064    1261    2039    2229    3021     919 
   Size:        145     197     778     190     792    2145

  Fragments arranged by size: 

                2145     792     778     197     190     145
 
BsmI GAATG_Cn'

Cuts at:    328     328 
   Size:       4247
 
BsmBI CGTCTCn'nnnn_

Cuts at:    988     988 
   Size:       4247
 
Bsp1286I G_dGCh'C

Cuts at:    319     460     640     919     985    1010    1064    1201    1261 
   Size:        141     180     279      66      25      54     137      60

Cuts at:   1261    1872    1965    2039    2229    2291    3021     319 
   Size:        611      93      74     190      62     730    1545

  Fragments arranged by size: 

                1545     730     611     279     190     180     141     137
                  93      74      66      62      60      54      25
 
BspEI T'CCGG_A

Cuts at:    245    3467     245 
   Size:       3222    1025
 
BspHI T'CATG_A

Cuts at:   3423    3423 
   Size:       4247
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    666    1297    1511    1813    2194     666 
   Size:        631     214     302     381    2719

  Fragments arranged by size: 

                2719     631     381     302     214
 
BsrBI CCG'CTC

Cuts at:     50    1103    2539    2593    4077    4242      50 
   Size:       1053    1436      54    1484     165      55

  Fragments arranged by size: 

                1484    1436    1053     165      55      54
 
BsrDI GCAATG_nn'

Cuts at:   2159    2159 
   Size:       4247
 
BsrFI r'CCGG_y

Cuts at:   2245    2426    2635    2245 
   Size:        181     209    3857

  Fragments arranged by size: 

                3857     209     181
 
BsrGI T'GTAC_A

Cuts at:    790     790 
   Size:       4247
 
BssHII G'CGCG_C

Cuts at:    345     345 
   Size:       4247
 
BssSI C'ACGA_G

Cuts at:   1030    2518    2876    1030 
   Size:       1488     358    2401

  Fragments arranged by size: 

                2401    1488     358
 
BstAPI GCAn_nnn'nTGC

Cuts at:    323    1624     323 
   Size:       1301    2946
 
BstBI TT'CG_AA

Cuts at:   2608    2608 
   Size:       4247
 
BstXI CCAn_nnnn'nTGG

Cuts at:    468     468 
   Size:       4247
 
BstYI r'GATC_y

Cuts at:      1     878    1272    1739    1762    2097    2343    3355    3441 
   Size:        877     394     467      23     335     246    1012      86

Cuts at:   3441    3453    3799       1 
   Size:         12     346     449

  Fragments arranged by size: 

                1012     877     467     449     394     346     335     246
                  86      23      12
 
Bsu36I CC'TnA_GG

Cuts at:    177     177 
   Size:       4247
 
BtgI C'CryG_G

Cuts at:    107    1001     107 
   Size:        894    3353
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:   2197    2378    2541    2197 
   Size:        181     163    3903

  Fragments arranged by size: 

                3903     181     163
 
BtsI GCAGTG_nn'

Cuts at:    823    1490     823 
   Size:        667    3580
 
DraI TTT'AAA

Cuts at:   1246    1246 
   Size:       4247
 
DrdI GACnn_nn'nnGTC

Cuts at:   1953    2811    1953 
   Size:        858    3389
 
EaeI y'GGCC_r

Cuts at:    470    1121    1832    2006    2397    2424    3769     470 
   Size:        651     711     174     391      27    1345     948

  Fragments arranged by size: 

                1345     948     711     651     391     174      27
 
EagI C'GGCC_G

Cuts at:   1832    1832 
   Size:       4247
 
EarI CTCTTCn'nnn_

Cuts at:    211     247    2270    2480     211 
   Size:         36    2023     210    1978

  Fragments arranged by size: 

                2023    1978     210      36
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:   2455    2765    2911    4057    2455 
   Size:        310     146    1146    2645

  Fragments arranged by size: 

                2645    1146     310     146
 
EcoICRI GAG'CTC

Cuts at:   1259    1259 
   Size:       4247
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:    148     322     444     920    2072    2504    3251    3519     148 
   Size:        174     122     476    1152     432     747     268     876

  Fragments arranged by size: 

                1152     876     747     476     432     268     174     122
            
 
EcoO109I rG'GnC_Cy

Cuts at:    465    1386     465 
   Size:        921    3326
 
EcoRI G'AATT_C

Cuts at:   1251    1251 
   Size:       4247
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:    258     972    2266     258 
   Size:        714    1294    2239

  Fragments arranged by size: 

                2239    1294     714
 
FspI TGC'GCA

Cuts at:   2028    2028 
   Size:       4247
 
HaeII r_GCGC'y

Cuts at:   1681    1929    2951    3930    1681 
   Size:        248    1022     979    1998

  Fragments arranged by size: 

                1998    1022     979     248
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:    295     327     901     933    1183    1215    2089    2119    2121 
   Size:         32     574      32     250      32     874      30       2

Cuts at:   2121    2151     295 
   Size:         30    2391

  Fragments arranged by size: 

                2391     874     574     250      32      32      32      30
                  30       2
 
HincII GTy'rAC

Cuts at:   1286    1286 
   Size:       4247
 
KasI G'GCGC_C

Cuts at:   1677    1925    1677 
   Size:        248    3999
 
KpnI G_GTAC'C

Cuts at:   1267    1267 
   Size:       4247
 
MluI A'CGCG_T

Cuts at:   3680    3680 
   Size:       4247
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:    844    2918    3102     844 
   Size:       2074     184    1989

  Fragments arranged by size: 

                2074    1989     184
 
MscI TGG'CCA

Cuts at:    472    1123    2008     472 
   Size:        651     885    2711

  Fragments arranged by size: 

                2711     885     651
 
MslI CAynn'nnrTG

Cuts at:    526     676     748    2236    2363     526 
   Size:        150      72    1488     127    2410

  Fragments arranged by size: 

                2410    1488     150     127      72
 
MspA1I CmG'CkG

Cuts at:    288     778    1358    1672    2032    3045    3290     288 
   Size:        490     580     314     360    1013     245    1245

  Fragments arranged by size: 

                1245    1013     580     490     360     314     245
 
NaeI GCC'GGC

Cuts at:   2428    2428 
   Size:       4247
 
NarI GG'CG_CC

Cuts at:   1678    1926    1678 
   Size:        248    3999
 
NcoI C'CATG_G

Cuts at:    107    1001     107 
   Size:        894    3353
 
NgoMIV G'CCGG_C

Cuts at:   2426    2426 
   Size:       4247
 
NspI r_CATG'y

Cuts at:    675    1300    1800    2707     675 
   Size:        625     500     907    2215

  Fragments arranged by size: 

                2215     907     625     500
 
PciI A'CATG_T

Cuts at:   2703    2703 
   Size:       4247
 
PflMI CCAn_nnn'nTGG

Cuts at:   1083    1083 
   Size:       4247
 
PfoI T'CCnGG_A

Cuts at:    593     593 
   Size:       4247
 
PmeI GTTT'AAAC

Cuts at:   1246    1246 
   Size:       4247
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3386    3418    3386 
   Size:         32    4215
 
PpuMI rG'GwC_Cy

Cuts at:    465     465 
   Size:       4247
 
PstI C_TGCA'G

Cuts at:    262    1294    1979     262 
   Size:       1032     685    2530

  Fragments arranged by size: 

                2530    1032     685
 
PvuI CG_AT'CG

Cuts at:    104     104 
   Size:       4247
 
PvuII CAG'CTG

Cuts at:    288     778    1672    2032     288 
   Size:        490     894     360    2503

  Fragments arranged by size: 

                2503     894     490     360
 
RsrII CG'GwC_CG

Cuts at:   2442    2442 
   Size:       4247
 
SacI G_AGCT'C

Cuts at:   1261    1261 
   Size:       4247
 
SalI G'TCGA_C

Cuts at:   1284    1284 
   Size:       4247
 
SapI GCTCTTCn'nnn_

Cuts at:    211     247    2270    2480     211 
   Size:         36    2023     210    1978

  Fragments arranged by size: 

                2023    1978     210      36
 
SbfI CC_TGCA'GG

Cuts at:   1294    1294 
   Size:       4247
 
ScaI AGT'ACT

Cuts at:    970    3764     970 
   Size:       2794    1453
 
SexAI A'CCwGG_T

Cuts at:    149     149 
   Size:       4247
 
SfcI C'TryA_G

Cuts at:     33     258     972    1290    1975    2968    3159      33 
   Size:        225     714     318     685     993     191    1121

  Fragments arranged by size: 

                1121     993     714     685     318     225     191
 
SfoI GGC'GCC

Cuts at:   1679    1927    1679 
   Size:        248    3999
 
SgfI GCG_AT'CGC

Cuts at:    104     104 
   Size:       4247
 
SmaI CCC'GGG

Cuts at:    685    1269     685 
   Size:        584    3663
 
SmlI C'TyrA_G

Cuts at:    279     355     706     759    1402    2317    2809    3071     279 
   Size:         76     351      53     643     915     492     262    1455

  Fragments arranged by size: 

                1455     915     643     492     351     262      76      53
            
 
SpeI A'CTAG_T

Cuts at:     67      67 
   Size:       4247
 
SphI G_CATG'C

Cuts at:    675    1300    1800     675 
   Size:        625     500    3122

  Fragments arranged by size: 

                3122     625     500
 
SrfI GCCC'GGGC

Cuts at:    685     685 
   Size:       4247
 
StyI C'CwwG_G

Cuts at:    107     461     689     731    1001    1381    1735     107 
   Size:        354     228      42     270     380     354    2619

  Fragments arranged by size: 

                2619     380     354     354     270     228      42
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:   1830    1963    2422    2631    1830 
   Size:        133     459     209    3446

  Fragments arranged by size: 

                3446     459     209     133
 
TatI w'GTAC_w

Cuts at:    790     968    3762     790 
   Size:        178    2794    1275

  Fragments arranged by size: 

                2794    1275     178
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   1161    1193    3764    3796    1161 
   Size:         32    2571      32    1612

  Fragments arranged by size: 

                2571    1612      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   2044    2044 
   Size:       4247
 
XbaI T'CTAG_A

Cuts at:   1278    1278 
   Size:       4247
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:    480     503    1742     480 
   Size:         23    1239    2985

  Fragments arranged by size: 

                2985    1239      23
 
XmaI C'CCGG_G

Cuts at:    683    1267     683 
   Size:        584    3663
 

 Enzymes that do cut:

     AarI     AccI   Acc65I     AclI     AcuI   AflIII     AgeI    AlwNI
    ApaLI     ApoI     AseI    AsiSI     AvaI    BamHI     BanI    BanII
     BbeI     BbsI    BciVI     BclI     BglI    BglII     BlpI Bme1580I
     BmrI     BplI     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseRI    BseYI     BsgI    BsiEI  BsiHKAI     BsmI
    BsmBI Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI    BstYI   Bsu36I
     BtgI    BtgZI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI EcoO109I    EcoRI     FalI     FspI    HaeII
    Hin4I   HincII     KasI     KpnI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NarI     NcoI   NgoMIV     NspI     PciI    PflMI
     PfoI     PmeI     PpiI    PpuMI     PstI     PvuI    PvuII    RsrII
     SacI     SalI     SapI     SbfI     ScaI    SexAI     SfcI     SfoI
     SgfI     SmaI     SmlI     SpeI     SphI     SrfI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XmaI

 Enzymes that do not cut: 

    AatII     AfeI    AflII     AhdI     AleI     AlfI     AloI     ApaI
     AscI    AvrII     BaeI    BbvCI     BcgI    BfrBI    BmgBI     BmtI
   Bpu10I    BsiWI   BstEII  BstZ17I     ClaI    CspCI   DraIII    EcoNI
    EcoRV     FseI    FspAI  HindIII     HpaI     MfeI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PasI     PmlI    PshAI     PsiI
   PspOMI    PspXI     PsrI    SacII    SanDI     SfiI    SgrAI    SnaBI
     SspI     StuI     SwaI     XhoI     XmnI     ZraI
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