Restriction map for pF1KB5307
##################################
# NO CUT ENZYMES (  108 enzymes) #
##################################

AclI        AcsI        AflII       AflIII      AgeI        
AhdI        Alw44I      AocI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     AspEI       
AviII       AvrII       BamHI       BbsI        BcgI        
BciVI       BfrI        BglI        BlnI        BlpI        
BpmI        Bpu1102I    BpuAI       BsiEI       BsiWI       
Bsp1407I    BspHI       BspLU11I    BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstBI       BstZ17I     
Bsu36I      CelII       Cfr10I      CpoI        DraI        
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco52I      Eco81I      EcoNI       EcoRI       EcoRV       
EcoT14I     FseI        FspI        HincII      HindII      
HpaI        Ksp632I     KspI        MfeI        MluI        
MseI        MunI        NaeI        NcoI        NdeI        
NgoMIV      NheI        NotI        NruI        NspV        
PacI        PaeR7I      PflMI       PinAI       PmeI        
PshAI       PshBI       Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
SexAI       SfiI        SfuI        SgrAI       SnaBI       
SpeI        SphI        SspBI       SspI        StuI        
StyI        SwaI        Tru9I       Tsp509I     Van91I      
XbaI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        275  GGTACC               G^GTACC
AccI         1186  GTCTAC               GT^[AC][GT]AC
AccIII       1229  TCCGGA               T^CCGGA
Aor51HI       134  AGCGCT               AGC^GCT
ApaI           83  GGGCCC               GGGCC^C
Asp718I       275  GGTACC               G^GTACC
BanII          83  GGGCCC               G[GA]GC[CT]^C
BbeI         1069  GGCGCC               GGCGC^C
BclI          926  TGATCA               T^GATCA
BfaI          865  CTAG                 C^TAG
BglII         969  AGATCT               A^GATCT
BsaBI         916  GATGTCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI         1145  GGTCTC               GGTCTC(1/5)
BsaWI        1229  TCCGGA               [AT]^CCGG[AT]
BseAI        1229  TCCGGA               T^CCGGA
BseRI          21  GAGGAG               GAGGAG(10/8)
BsgI          367  CTGCAC               GTGCAG(16/14)
BsmBI         670  CGTCTC               CGTCTC(1/5)
BsmI          834  GAATGC               GAATGC(1/-1)
BspDI        1207  ATCGAT               AT^CGAT
BspEI        1229  TCCGGA               T^CCGGA
BsrDI         740  GCAATG               GCAATG(2/0)
BstAPI       1158  GCAGTCCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII        495  GGTGACC              G^GT[ACGT]ACC
BstPI         495  GGTGACC              G^GT[ACGT]ACC
BstXI        1203  CCACATCGATGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI         1207  ATCGAT               AT^CGAT
DraIII        112  CACCACGTG            CAC[ACGT][ACGT][ACGT]^GTG
DsaI          945  CCGTGG               C^C[GA][CT]GG
Eco47III      134  AGCGCT               AGC^GCT
EcoO65I       495  GGTGACC              G^GT[ACGT]ACC
EcoT22I      1060  ATGCAT               ATGCA^T
FbaI          926  TGATCA               T^GATCA
HindIII      1224  AAGCTT               A^AGCTT
KasI         1069  GGCGCC               G^GCGCC
KpnI          275  GGTACC               GGTAC^C
MaeI          865  CTAG                 C^TAG
MamI          916  GATGTCGATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboII         684  GAAGA                GAAGA(8/7)
MroI         1229  TCCGGA               T^CCGGA
NarI         1069  GGCGCC               GG^CGCC
NsiI         1060  ATGCAT               ATGCA^T
PleI          858  GAGTC                GAGTC(4/5)
SbfI         1164  CCTGCAGG             CCTGCA^GG
ScaI          449  AGTACT               AGT^ACT
SfoI         1069  GGCGCC               GGC^GCC
SmaI           31  CCCGGG               CCC^GGG
SmlI          829  CTCAAG               C^T[CT][GA]AG
Sse8387I     1164  CCTGCAGG             CCTGCA^GG
XcmI          611  CCACGCATCACCTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI          31  CCCGGG               C^CCGGG
XmaI           31  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AatII          93  GACGTC               GACGT^C
             1183  GACGTC              
AspHI         269  GTGCTC               G[AT]GC[AT]^C
              727  GAGCAC              
AspI          646  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              976  GACCGGGTC           
AvaI           31  CCCGGG               C^[CT]CG[GA]G
              128  CTCGGG              
BanI          275  GGTACC               G^G[CT][GA]CC
             1069  GGCGCC              
BbrPI         115  CACGTG               CAC^GTG
              370  CACGTG              
BbvCI          18  GCTGAGG              CCTCAGC(-5/-2)
             1006  GCTGAGG             
BsiHKAI       269  GTGCTC               G[AT]GC[AT]^C
              727  GAGCAC              
BsmAI         671  GTCTC                GTCTC(1/5)
             1146  GTCTC               
BsoBI          31  CCCGGG               C^[CT]CG[GA]G
              128  CTCGGG              
BspMI         278  ACCTGC               ACCTGC(4/8)
             1190  ACCTGC              
BstYI         935  GGATCT               [GA]^GATC[CT]
              969  AGATCT              
MflI          935  GGATCT               [GA]^GATC[CT]
              969  AGATCT              
NspI          602  GCATGT               [GA]CATG^[CT]
             1058  ACATGC              
PflFI         646  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              976  GACCGGGTC           
PmaCI         115  CACGTG               CAC^GTG
              370  CACGTG              
PmlI          115  CACGTG               CAC^GTG
              370  CACGTG              
TfiI          596  GAATC                G^A[AT]TC
              956  GAATC               
TspRI         296  CAGTG                CA[GC]TG[ACGT][ACGT]^
              803  CACTG               
Tth111I       646  GACTTTGTC            GAC[ACGT]^[ACGT][ACGT]GTC
              976  GACCGGGTC           
XhoII         935  GGATCT               [GA]^GATC[CT]
              969  AGATCT              


#########################################
# 3 OR MORE CUT ENZYMES (   77 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwI        AlwNI       AvaII       BalI        BbvI        
BcnI        BmyI        BsaAI       BsaHI       BsaJI       
BsiYI       BslI        BsmFI       Bsp1286I    BsrBI       
BsrI        BssKI       BstF5I      BstNI       BstUI       
Cac8I       CfoI        Cfr13I      DdeI        DpnII       
DraII       EaeI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        MaeII       MaeIII      
MboI        MluNI       MnlI        MscI        MslI        
MspA1I      MspI        MvaI        MvnI        MwoI        
NciI        NdeII       NlaIII      NlaIV       PpuMI       
PstI        PvuII       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        TaqI        TseI        
Tsp45I      TthHB8I     
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