Restriction map for pF1KB6110
 (Linear) (Six-base) MAPSORT of: KIBB6110.seq  Check: 3204  from: 1  to: 1884



KIBB6110.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:19  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0    1033    1043    1560    1884 
   Size:       1033      10     517     324

  Fragments arranged by size: 

                1033     517     324      10
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     154    1588    1884 
   Size:        154    1434     296

  Fragments arranged by size: 

                1434     296     154
 
AflIII A'CryG_T

Cuts at:      0    1093    1884 
   Size:       1093     791
 
AhdI GACnn_n'nnGTC

Cuts at:      0    1613    1856    1884 
   Size:       1613     243      28

  Fragments arranged by size: 

                1613     243      28
 
AleI CACnn'nnGTG

Cuts at:      0     772     975    1884 
   Size:        772     203     909

  Fragments arranged by size: 

                 909     772     203
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     857    1884 
   Size:        857    1027
 
AlwNI CAG_nnn'CTG

Cuts at:      0     757    1884 
   Size:        757    1127
 
ApaI G_GGCC'C

Cuts at:      0     215     325    1714    1884 
   Size:        215     110    1389     170

  Fragments arranged by size: 

                1389     215     170     110
 
ApaLI G'TGCA_C

Cuts at:      0     367    1884 
   Size:        367    1517
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:      0    1528    1561    1884 
   Size:       1528      33     323

  Fragments arranged by size: 

                1528     323      33
 
BamHI G'GATC_C

Cuts at:      0    1207    1884 
   Size:       1207     677
 
BanI G'GyrC_C

Cuts at:      0     247     310    1214    1758    1884 
   Size:        247      63     904     544     126

  Fragments arranged by size: 

                 904     544     247     126      63
 
BanII G_rGCy'C

Cuts at:      0      56     110     215     325     422     704    1492    1714 
   Size:         56      54     105     110      97     282     788     222

Cuts at:   1714    1738    1884 
   Size:         24     146

  Fragments arranged by size: 

                 788     282     222     146     110     105      97      56
                  54      24
 
BbeI G_GCGC'C

Cuts at:      0    1762    1884 
   Size:       1762     122
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     208    1884 
   Size:        208    1676
 
BbvCI CC'TCA_GC

Cuts at:      0    1413    1884 
   Size:       1413     471
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     889    1884 
   Size:        889     995
 
BclI T'GATC_A

Cuts at:      0     908    1884 
   Size:        908     976
 
BglI GCCn_nnn'nGGC

Cuts at:      0     708    1426    1756    1884 
   Size:        708     718     330     128

  Fragments arranged by size: 

                 718     708     330     128
 
Bme1580I G_kGCm'C

Cuts at:      0     150     215     325     371     661     906     982    1020 
   Size:        150      65     110      46     290     245      76      38

Cuts at:   1020    1219    1504    1714    1884 
   Size:        199     285     210     170

  Fragments arranged by size: 

                 290     285     245     210     199     170     150     110
                  76      65      46      38
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1288    1573    1884 
   Size:       1288     285     311

  Fragments arranged by size: 

                1288     311     285
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40    1267    1884 
   Size:         40    1227     617

  Fragments arranged by size: 

                1227     617      40
 
Bpu10I CC'TnA_GC

Cuts at:      0     574    1413    1483    1884 
   Size:        574     839      70     401

  Fragments arranged by size: 

                 839     574     401      70
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1827    1884 
   Size:       1827      57
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     586    1884 
   Size:        586    1298
 
BsaBI GATnn'nnATC

Cuts at:      0     166    1884 
   Size:        166    1718
 
BsaHI Gr'CG_yC

Cuts at:      0    1759    1884 
   Size:       1759     125
 
BsaWI w'CCGG_w

Cuts at:      0     881    1884 
   Size:        881    1003
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0    1387    1884 
   Size:       1387     497
 
BseYI C'CCAG_C

Cuts at:      0       9     318     359     497     712    1728    1884 
   Size:          9     309      41     138     215    1016     156

  Fragments arranged by size: 

                1016     309     215     156     138      41       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     109     526     589    1884 
   Size:        109     417      63    1295

  Fragments arranged by size: 

                1295     417     109      63
 
BsiHKAI G_wGCw'C

Cuts at:      0      56     110     371     422    1738    1884 
   Size:         56      54     261      51    1316     146

  Fragments arranged by size: 

                1316     261     146      56      54      51
 
Bsp1286I G_dGCh'C

Cuts at:      0      56     110     150     215     325     371     422     661 
   Size:         56      54      40      65     110      46      51     239

Cuts at:    661     704     906     982    1020    1219    1492    1504    1714 
   Size:         43     202      76      38     199     273      12     210

Cuts at:   1714    1738    1884 
   Size:         24     146

  Fragments arranged by size: 

                 273     239     210     202     199     146     110      76
                  65      56      54      51      46      43      40      38
                  24      12
 
BspEI T'CCGG_A

Cuts at:      0     881    1884 
   Size:        881    1003
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1033    1043    1560    1884 
   Size:       1033      10     517     324

  Fragments arranged by size: 

                1033     517     324      10
 
BsrDI GCAATG_nn'

Cuts at:      0     391    1884 
   Size:        391    1493
 
BssSI C'ACGA_G

Cuts at:      0     103    1884 
   Size:        103    1781
 
BstEII G'GTnAC_C

Cuts at:      0     670    1884 
   Size:        670    1214
 
BstYI r'GATC_y

Cuts at:      0    1187    1207    1308    1377    1884 
   Size:       1187      20     101      69     507

  Fragments arranged by size: 

                1187     507     101      69      20
 
Bsu36I CC'TnA_GG

Cuts at:      0    1836    1884 
   Size:       1836      48
 
BtsI GCAGTG_nn'

Cuts at:      0    1740    1884 
   Size:       1740     144
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     420     455    1884 
   Size:        420      35    1429

  Fragments arranged by size: 

                1429     420      35
 
DraIII CAC_nnn'GTG

Cuts at:      0      30    1884 
   Size:         30    1854
 
EaeI y'GGCC_r

Cuts at:      0      78     113     947    1461    1668    1770    1884 
   Size:         78      35     834     514     207     102     114

  Fragments arranged by size: 

                 834     514     207     114     102      78      35
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      19    1884 
   Size:         19    1865
 
EcoICRI GAG'CTC

Cuts at:      0      54     108     420    1736    1884 
   Size:         54      54     312    1316     148

  Fragments arranged by size: 

                1316     312     148      54      54
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      40     154    1267    1588    1884 
   Size:         40     114    1113     321     296

  Fragments arranged by size: 

                1113     321     296     114      40
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     115    1884 
   Size:        115    1769
 
EcoO109I rG'GnC_Cy

Cuts at:      0     211     212     322    1691    1802    1825    1840    1884 
   Size:        211       1     110    1369     111      23      15      44

  Fragments arranged by size: 

                1369     211     111     110      44      23      15       1
            
 
HaeII r_GCGC'y

Cuts at:      0    1751    1762    1884 
   Size:       1751      11     122

  Fragments arranged by size: 

                1751     122      11
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0    1507    1539    1559    1591    1599    1631    1683    1715 
   Size:       1507      32      20      32       8      32      52      32

Cuts at:   1715    1842    1874    1884 
   Size:        127      32      10

  Fragments arranged by size: 

                1507     127      52      32      32      32      32      32
                  20      10       8
 
HincII GTy'rAC

Cuts at:      0     839    1884 
   Size:        839    1045
 
KasI G'GCGC_C

Cuts at:      0    1758    1884 
   Size:       1758     126
 
MfeI C'AATT_G

Cuts at:      0     780    1884 
   Size:        780    1104
 
MscI TGG'CCA

Cuts at:      0     115     949    1463    1670    1884 
   Size:        115     834     514     207     214

  Fragments arranged by size: 

                 834     514     214     207     115
 
MslI CAynn'nnrTG

Cuts at:      0      31     374     565     772     975    1092    1884 
   Size:         31     343     191     207     203     117     792

  Fragments arranged by size: 

                 792     343     207     203     191     117      31
 
MspA1I CmG'CkG

Cuts at:      0     143     459     599    1417    1884 
   Size:        143     316     140     818     467

  Fragments arranged by size: 

                 818     467     316     143     140
 
NarI GG'CG_CC

Cuts at:      0    1759    1884 
   Size:       1759     125
 
NspI r_CATG'y

Cuts at:      0     345    1097    1884 
   Size:        345     752     787

  Fragments arranged by size: 

                 787     752     345
 
PasI CC'CwG_GG

Cuts at:      0     207    1795    1884 
   Size:        207    1588      89

  Fragments arranged by size: 

                1588     207      89
 
PciI A'CATG_T

Cuts at:      0    1093    1884 
   Size:       1093     791
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1197    1267    1884 
   Size:       1197      70     617

  Fragments arranged by size: 

                1197     617      70
 
PpuMI rG'GwC_Cy

Cuts at:      0    1691    1825    1840    1884 
   Size:       1691     134      15      44

  Fragments arranged by size: 

                1691     134      44      15
 
PspOMI G'GGCC_C

Cuts at:      0     211     321    1710    1884 
   Size:        211     110    1389     174

  Fragments arranged by size: 

                1389     211     174     110
 
PstI C_TGCA'G

Cuts at:      0     846    1040    1747    1884 
   Size:        846     194     707     137

  Fragments arranged by size: 

                 846     707     194     137
 
PvuII CAG'CTG

Cuts at:      0     143     599    1417    1884 
   Size:        143     456     818     467

  Fragments arranged by size: 

                 818     467     456     143
 
SacI G_AGCT'C

Cuts at:      0      56     110     422    1738    1884 
   Size:         56      54     312    1316     146

  Fragments arranged by size: 

                1316     312     146      56      54
 
SanDI GG'GwC_CC

Cuts at:      0    1691    1884 
   Size:       1691     193
 
SexAI A'CCwGG_T

Cuts at:      0     824    1884 
   Size:        824    1060
 
SfcI C'TryA_G

Cuts at:      0     257     842    1036    1063    1743    1884 
   Size:        257     585     194      27     680     141

  Fragments arranged by size: 

                 680     585     257     194     141      27
 
SfoI GGC'GCC

Cuts at:      0    1760    1884 
   Size:       1760     124
 
SmlI C'TyrA_G

Cuts at:      0    1806    1884 
   Size:       1806      78
 
StyI C'CwwG_G

Cuts at:      0     116    1578    1665    1884 
   Size:        116    1462      87     219

  Fragments arranged by size: 

                1462     219     116      87
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1498    1850    1884 
   Size:       1498     352      34

  Fragments arranged by size: 

                1498     352      34
 
TatI w'GTAC_w

Cuts at:      0    1286    1884 
   Size:       1286     598
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      75     107    1456    1488    1541    1573    1884 
   Size:         75      32    1349      32      53      32     311

  Fragments arranged by size: 

                1349     311      75      53      32      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      64     333     771    1884 
   Size:         64     269     438    1113

  Fragments arranged by size: 

                1113     438     269      64
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AleI     AlfI    AlwNI     ApaI
    ApaLI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
    BciVI     BclI     BglI Bme1580I     BmrI     BpmI   Bpu10I    BpuEI
     BsaI    BsaBI    BsaHI    BsaWI    BseRI    BseYI     BsgI  BsiHKAI
 Bsp1286I    BspEI    BspMI    BsrDI    BssSI   BstEII    BstYI   Bsu36I
     BtsI    CspCI   DraIII     EaeI     EciI  EcoICRI  Eco57MI    EcoNI
 EcoO109I    HaeII    Hin4I   HincII     KasI     MfeI     MscI     MslI
   MspA1I     NarI     NspI     PasI     PciI    PflMI    PpuMI   PspOMI
     PstI    PvuII     SacI    SanDI    SexAI     SfcI     SfoI     SmlI
     StyI    TaqII     TatI     TstI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AloI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BcgI    BfrBI
    BglII     BlpI    BmgBI     BmtI     BplI    BsaAI    BsaXI    BsiEI
    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrFI    BsrGI   BssHII
   BstAPI    BstBI    BstXI  BstZ17I     BtgI    BtgZI     ClaI     DraI
     DrdI     EagI     EarI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI  HindIII     HpaI     KpnI     MluI     MmeI     NaeI     NcoI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI    PspXI     PsrI     PvuI
    RsrII    SacII     SalI     SapI     SbfI     ScaI     SfiI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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