Restriction map for pF1KE1394
 (Linear) (Six-base) MAPSORT of: KIEE1394.seq  Check: 3056  from: 1  to: 1566



KIEE1394.orf

 With 182 enzymes: SgfI * 

                        January 28, 2010 13:22  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     672    1152    1566 
   Size:        672     480     414

  Fragments arranged by size: 

                 672     480     414
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1064    1071    1256    1471    1566 
   Size:       1064       7     185     215      95

  Fragments arranged by size: 

                1064     215     185      95       7
 
AflII C'TTAA_G

Cuts at:      0     516    1566 
   Size:        516    1050
 
AleI CACnn'nnGTG

Cuts at:      0     707    1566 
   Size:        707     859
 
AlwNI CAG_nnn'CTG

Cuts at:      0     673    1566 
   Size:        673     893
 
ApoI r'AATT_y

Cuts at:      0      46     152    1302    1566 
   Size:         46     106    1150     264

  Fragments arranged by size: 

                1150     264     106      46
 
BamHI G'GATC_C

Cuts at:      0     197     717    1566 
   Size:        197     520     849

  Fragments arranged by size: 

                 849     520     197
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     599    1010    1566 
   Size:        599     411     556

  Fragments arranged by size: 

                 599     556     411
 
BfrBI ATG'CAT

Cuts at:      0      99    1566 
   Size:         99    1467
 
BglI GCCn_nnn'nGGC

Cuts at:      0     247    1566 
   Size:        247    1319
 
BglII A'GATC_T

Cuts at:      0     484    1566 
   Size:        484    1082
 
BmgBI CAC'GTC

Cuts at:      0     921    1566 
   Size:        921     645
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     754    1566 
   Size:        754     812
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167    1255    1566 
   Size:        167    1088     311

  Fragments arranged by size: 

                1088     311     167
 
BsaAI yAC'GTr

Cuts at:      0     684    1566 
   Size:        684     882
 
BsaBI GATnn'nnATC

Cuts at:      0     196    1566 
   Size:        196    1370
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     165     195     287     317    1566 
   Size:        165      30      92      30    1249

  Fragments arranged by size: 

                1249     165      92      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     170     898    1252    1566 
   Size:        170     728     354     314

  Fragments arranged by size: 

                 728     354     314     170
 
BsiHKAI G_wGCw'C

Cuts at:      0    1143    1566 
   Size:       1143     423
 
Bsp1286I G_dGCh'C

Cuts at:      0    1143    1566 
   Size:       1143     423
 
BspHI T'CATG_A

Cuts at:      0     752    1566 
   Size:        752     814
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     672    1152    1566 
   Size:        672     480     414

  Fragments arranged by size: 

                 672     480     414
 
BsrDI GCAATG_nn'

Cuts at:      0      81     227     446     731    1494    1566 
   Size:         81     146     219     285     763      72

  Fragments arranged by size: 

                 763     285     219     146      81      72
 
BsrFI r'CCGG_y

Cuts at:      0    1035    1566 
   Size:       1035     531
 
BsrGI T'GTAC_A

Cuts at:      0    1292    1566 
   Size:       1292     274
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     744    1566 
   Size:        744     822
 
BstYI r'GATC_y

Cuts at:      0     197     484     613     717    1465    1566 
   Size:        197     287     129     104     748     101

  Fragments arranged by size: 

                 748     287     197     129     104     101
 
BtgI C'CryG_G

Cuts at:      0     428     965    1566 
   Size:        428     537     601

  Fragments arranged by size: 

                 601     537     428
 
EaeI y'GGCC_r

Cuts at:      0     425    1566 
   Size:        425    1141
 
EarI CTCTTCn'nnn_

Cuts at:      0     981    1149    1409    1566 
   Size:        981     168     260     157

  Fragments arranged by size: 

                 981     260     168     157
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     167    1064    1071    1255    1256    1471    1566 
   Size:        167     897       7     184       1     215      95

  Fragments arranged by size: 

                 897     215     184     167      95       7       1
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     317    1566 
   Size:        317    1249
 
EcoRI G'AATT_C

Cuts at:      0      46    1566 
   Size:         46    1520
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      98     130     165     197    1566 
   Size:         98      32      35      32    1369

  Fragments arranged by size: 

                1369      98      35      32      32
 
HincII GTy'rAC

Cuts at:      0     957    1566 
   Size:        957     609
 
MfeI C'AATT_G

Cuts at:      0     569    1566 
   Size:        569     997
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0    1521    1566 
   Size:       1521      45
 
MscI TGG'CCA

Cuts at:      0     427    1566 
   Size:        427    1139
 
MslI CAynn'nnrTG

Cuts at:      0     217     553     707     857    1566 
   Size:        217     336     154     150     709

  Fragments arranged by size: 

                 709     336     217     154     150
 
MspA1I CmG'CkG

Cuts at:      0    1109    1566 
   Size:       1109     457
 
NcoI C'CATG_G

Cuts at:      0     428     965    1566 
   Size:        428     537     601

  Fragments arranged by size: 

                 601     537     428
 
NsiI A_TGCA'T

Cuts at:      0     101    1566 
   Size:        101    1465
 
NspI r_CATG'y

Cuts at:      0     103    1566 
   Size:        103    1463
 
PfoI T'CCnGG_A

Cuts at:      0     183    1566 
   Size:        183    1383
 
PsiI TTA'TAA

Cuts at:      0     481    1566 
   Size:        481    1085
 
PstI C_TGCA'G

Cuts at:      0     669     909    1473    1566 
   Size:        669     240     564      93

  Fragments arranged by size: 

                 669     564     240      93
 
SapI GCTCTTCn'nnn_

Cuts at:      0    1149    1566 
   Size:       1149     417
 
SfcI C'TryA_G

Cuts at:      0     665     905    1318    1398    1469    1566 
   Size:        665     240     413      80      71      97

  Fragments arranged by size: 

                 665     413     240      97      80      71
 
SmlI C'TyrA_G

Cuts at:      0     516    1566 
   Size:        516    1050
 
SspI AAT'ATT

Cuts at:      0     834    1566 
   Size:        834     732
 
StuI AGG'CCT

Cuts at:      0      12      39    1566 
   Size:         12      27    1527

  Fragments arranged by size: 

                1527      27      12
 
StyI C'CwwG_G

Cuts at:      0     428     965    1566 
   Size:        428     537     601

  Fragments arranged by size: 

                 601     537     428
 
TatI w'GTAC_w

Cuts at:      0     741    1292    1418    1566 
   Size:        741     551     126     148

  Fragments arranged by size: 

                 741     551     148     126
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     668     700    1023    1055    1566 
   Size:        668      32     323      32     511

  Fragments arranged by size: 

                 668     511     323      32      32
 
XmnI GAAnn'nnTTC

Cuts at:      0     155    1049    1566 
   Size:        155     894     517

  Fragments arranged by size: 

                 894     517     155
 

 Enzymes that do cut:

     AarI     AcuI    AflII     AleI    AlwNI     ApoI    BamHI     BbsI
    BfrBI     BglI    BglII    BmgBI     BmrI     BpmI    BsaAI    BsaBI
    BsaXI    BseRI  BsiHKAI Bsp1286I    BspHI    BspMI    BsrDI    BsrFI
    BsrGI    BstXI    BstYI     BtgI     EaeI     EarI  Eco57MI    EcoNI
    EcoRI    Hin4I   HincII     MfeI     MmeI     MscI     MslI   MspA1I
     NcoI     NsiI     NspI     PfoI     PsiI     PstI     SapI     SfcI
     SmlI     SspI     StuI     StyI     TatI     TstI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI     AhdI
     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI     BanI    BanII     BbeI    BbvCI     BcgI    BciVI
     BclI     BlpI Bme1580I     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI
    BspEI    BsrBI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EagI     EciI  EcoICRI EcoO109I    EcoRV     FalI     FseI     FspI
    FspAI    HaeII  HindIII     HpaI     KasI     KpnI     MluI     NaeI
     NarI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI   PspOMI
    PspXI     PsrI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SwaI    TaqII  Tth111I
     XbaI     XcmI     XhoI     XmaI     ZraI
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