Restriction map for pF1KB5907
 (Linear) (Six-base) MAPSORT of: KIBB5907.seq  Check: 6321  from: 1  to: 1512



KIBB5907.orf

 With 182 enzymes: SgfI * 

                        October 7, 2009 14:18  ..

AccI GT'mk_AC

Cuts at:      0     145    1512 
   Size:        145    1367
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     135     664     680    1512 
   Size:        135     529      16     832

  Fragments arranged by size: 

                 832     529     135      16
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      39    1512 
   Size:         39    1473
 
AlwNI CAG_nnn'CTG

Cuts at:      0      60     642    1512 
   Size:         60     582     870

  Fragments arranged by size: 

                 870     582      60
 
ApaLI G'TGCA_C

Cuts at:      0      14    1512 
   Size:         14    1498
 
ApoI r'AATT_y

Cuts at:      0     615     677    1512 
   Size:        615      62     835

  Fragments arranged by size: 

                 835     615      62
 
AvaI C'yCGr_G

Cuts at:      0     563     575     990    1512 
   Size:        563      12     415     522

  Fragments arranged by size: 

                 563     522     415      12
 
AvrII C'CTAG_G

Cuts at:      0     955    1512 
   Size:        955     557
 
BanI G'GyrC_C

Cuts at:      0     364    1195    1300    1512 
   Size:        364     831     105     212

  Fragments arranged by size: 

                 831     364     212     105
 
BanII G_rGCy'C

Cuts at:      0     694    1512 
   Size:        694     818
 
BbvCI CC'TCA_GC

Cuts at:      0    1329    1512 
   Size:       1329     183
 
BglI GCCn_nnn'nGGC

Cuts at:      0     698    1512 
   Size:        698     814
 
BlpI GC'TnA_GC

Cuts at:      0     104    1512 
   Size:        104    1408
 
Bme1580I G_kGCm'C

Cuts at:      0      18     367    1512 
   Size:         18     349    1145

  Fragments arranged by size: 

                1145     349      18
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     778    1512 
   Size:        778     734
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     274     304     475     579    1103    1465    1512 
   Size:        274      30     171     104     524     362      47

  Fragments arranged by size: 

                 524     362     274     171     104      47      30
 
Bpu10I CC'TnA_GC

Cuts at:      0     109    1329    1512 
   Size:        109    1220     183

  Fragments arranged by size: 

                1220     183     109
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     328     809     841    1512 
   Size:        328     481      32     671

  Fragments arranged by size: 

                 671     481     328      32
 
BsaAI yAC'GTr

Cuts at:      0     611     854    1512 
   Size:        611     243     658

  Fragments arranged by size: 

                 658     611     243
 
BsaWI w'CCGG_w

Cuts at:      0     727    1512 
   Size:        727     785
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     728     758     838     868    1512 
   Size:        728      30      80      30     644

  Fragments arranged by size: 

                 728     644      80      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     283     424    1512 
   Size:        283     141    1088

  Fragments arranged by size: 

                1088     283     141
 
BseYI C'CCAG_C

Cuts at:      0     360    1512 
   Size:        360    1152
 
BsiHKAI G_wGCw'C

Cuts at:      0      18    1512 
   Size:         18    1494
 
Bsp1286I G_dGCh'C

Cuts at:      0      18     367     694    1512 
   Size:         18     349     327     818

  Fragments arranged by size: 

                 818     349     327      18
 
BsrBI CCG'CTC

Cuts at:      0     381    1512 
   Size:        381    1131
 
BsrGI T'GTAC_A

Cuts at:      0    1307    1512 
   Size:       1307     205
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     411     507    1512 
   Size:        411      96    1005

  Fragments arranged by size: 

                1005     411      96
 
BstZ17I GTA'TAC

Cuts at:      0     146    1512 
   Size:        146    1366
 
Bsu36I CC'TnA_GG

Cuts at:      0     370    1512 
   Size:        370    1142
 
BtgI C'CryG_G

Cuts at:      0    1095    1512 
   Size:       1095     417
 
BtsI GCAGTG_nn'

Cuts at:      0     569    1512 
   Size:        569     943
 
EaeI y'GGCC_r

Cuts at:      0     132     158     497    1512 
   Size:        132      26     339    1015

  Fragments arranged by size: 

                1015     339     132      26
 
EarI CTCTTCn'nnn_

Cuts at:      0     455    1512 
   Size:        455    1057
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     874    1512 
   Size:        874     638
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     135     274     304     475     579     664     680    1103 
   Size:        135     139      30     171     104      85      16     423

Cuts at:   1103    1465    1512 
   Size:        362      47

  Fragments arranged by size: 

                 423     362     171     139     135     104      85      47
                  30      16
 
EcoO109I rG'GnC_Cy

Cuts at:      0     326    1512 
   Size:        326    1186
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1473    1512 
   Size:       1473      39
 
HaeII r_GCGC'y

Cuts at:      0    1016    1512 
   Size:       1016     496
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     679     711    1512 
   Size:        679      32     801

  Fragments arranged by size: 

                 801     679      32
 
HincII GTy'rAC

Cuts at:      0    1347    1512 
   Size:       1347     165
 
MfeI C'AATT_G

Cuts at:      0     301    1512 
   Size:        301    1211
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     392     488    1512 
   Size:        392      96    1024

  Fragments arranged by size: 

                1024     392      96
 
MscI TGG'CCA

Cuts at:      0     160     499    1512 
   Size:        160     339    1013

  Fragments arranged by size: 

                1013     339     160
 
MspA1I CmG'CkG

Cuts at:      0      49      57     179     390    1512 
   Size:         49       8     122     211    1122

  Fragments arranged by size: 

                1122     211     122      49       8
 
PfoI T'CCnGG_A

Cuts at:      0     635    1512 
   Size:        635     877
 
PmlI CAC'GTG

Cuts at:      0     854    1512 
   Size:        854     658
 
PstI C_TGCA'G

Cuts at:      0      28     353    1512 
   Size:         28     325    1159

  Fragments arranged by size: 

                1159     325      28
 
PvuII CAG'CTG

Cuts at:      0      49      57     179     390    1512 
   Size:         49       8     122     211    1122

  Fragments arranged by size: 

                1122     211     122      49       8
 
SfcI C'TryA_G

Cuts at:      0      24     349     423    1512 
   Size:         24     325      74    1089

  Fragments arranged by size: 

                1089     325      74      24
 
SpeI A'CTAG_T

Cuts at:      0     741    1512 
   Size:        741     771
 
StuI AGG'CCT

Cuts at:      0    1160    1512 
   Size:       1160     352
 
StyI C'CwwG_G

Cuts at:      0     308     955    1512 
   Size:        308     647     557

  Fragments arranged by size: 

                 647     557     308
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     253     856    1512 
   Size:        253     603     656

  Fragments arranged by size: 

                 656     603     253
 
TatI w'GTAC_w

Cuts at:      0     986    1307    1415    1512 
   Size:        986     321     108      97

  Fragments arranged by size: 

                 986     321     108      97
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     838     870    1512 
   Size:        838      32     642

  Fragments arranged by size: 

                 838     642      32
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI    ApaLI     ApoI     AvaI    AvrII
     BanI    BanII    BbvCI     BglI     BlpI Bme1580I     BplI     BpmI
   Bpu10I     BsaI    BsaAI    BsaWI    BsaXI    BseRI    BseYI  BsiHKAI
 Bsp1286I    BsrBI    BsrGI    BstXI  BstZ17I   Bsu36I     BtgI     BtsI
     EaeI     EarI     EciI  Eco57MI EcoO109I     FalI    HaeII    Hin4I
   HincII     MfeI     MmeI     MscI   MspA1I     PfoI     PmlI     PstI
    PvuII     SfcI     SpeI     StuI     StyI    TaqII     TatI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI     AscI     AseI    AsiSI     BaeI
    BamHI     BbeI     BbsI     BcgI    BciVI     BclI    BfrBI    BglII
    BmgBI     BmrI     BmtI    BpuEI    BsaBI    BsaHI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrDI    BsrFI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MluI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SphI     SrfI     SspI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI
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